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The map label for this gene is ilvB [H]

Identifier: 145295410

GI number: 145295410

Start: 1480917

End: 1482776

Strand: Direct

Name: ilvB [H]

Synonym: cgR_1347

Alternate gene names: 145295410

Gene position: 1480917-1482776 (Clockwise)

Preceding gene: 145295405

Following gene: 145295411

Centisome position: 44.68

GC content: 57.26

Gene sequence:

>1860_bases
GTGAATGTGGCAGCTTCTCAACAGCCCACTCCCGCCACGGTTGCAAGCCGTGGTCGATCCGCCGCCCCTGAGCGGATGAC
AGGTGCACAGGCAATTGTTCGATCGCTCGAGGAGCTTAACGCCGACATCGTGTTCGGTATTCCTGGTGGTGCGGTGCTAC
CGGTGTATGACCCGCTCTATTCCTCCACAAAGGTGCGCCACGTCTTGGTGCGCCACGAGCAGGGCGCAGGCCACGCAGCA
ACCGGCTACGCGCAGGTTACTGGACGCGTTGGCGTCTGCATTGCAACCTCTGGCCCAGGAGCAACCAACTTGGTTACCCC
AATCGCTGATGCAAACTTGGACTCCGTTCCCATGGTTGCCATCACCGGCCAGGTCGGAAGTGGCCTGCTGGGTACCGACG
CTTTCCAGGAAGCCGATATCCGCGGCATCACCATGCCAGTGACCAAGCACAACTTCATGGTCACCGACCCCAACGACATT
CCACAGGCATTGGCTGAGGCATTCCACCTCGCGATTACTGGTCGCCCTGGCCCTGTTCTGGTGGATATTCCTAAGGATGT
CCAGAACGCTGAATTGGATTTCGTCTGGCCACCAAAGATCGACCTGCCAGGCTACCGCCCAGTTTCAACACCACATGCTC
GCCAGATCGAGCAGGCAGTCAAGCTGATCGGTGAGGCCAAGAAGCCCGTCCTTTACGTTGGAGGCGGCGTTATCAAGGCT
GACGCACACGAAGAGCTTCGTGCGTTCGCTGAGTACACCGGCATCCCAGTTGTCACCACCTTGATGGCTTTGGGTACTTT
CCCAGAGTCTCACGAGCTGCACATGGGTATGCCAGGCATGCATGGCACTGTGTCCGCTGTTGGTGCACTGCAGCGCAGCG
ACCTGCTGATTGCTATCGGCTCCCGCTTTGATGACCGCGTCACCGGTGACGTTGACACCTTCGCGCCTGACGCCAAGATC
ATTCACGCCGATATTGATCCTGCCGAAATCGGAAAGATCAAGCAGGTTGAGGTTCCAATCGTGGGCGATGCCCGCGAAGT
TCTTGCTCGTCTGCTGGAAACCACCAAGGCAAGCAAGGCAGAGACCGAGGACATCTCCGAGTGGGTTGACTACCTCAAGG
GCCTCAAGGCACGTTTCCCACGTGGCTACGACGAGCAGCCAGGCGATCTGCTGGCACCACAGTTTGTCATTGAAACCCTG
TCCAAGGAAGTTGGCCCCGACGCAATTTACTGCGCCGGCGTCGGACAGCACCAAATGTGGGCAGCTCAGTTCGTTGACTT
TGAAAAGCCACGCACCTGGCTCAACTCCGGTGGACTGGGCACCATGGGCTACGCAGTTCCTGCGGCCCTTGGAGCAAAGG
CTGGCGCACCTGACAAGGAAGTCTGGGCTATCGACGGCGACGGCTGTTTCCAGATGACCAACCAGGAACTCACCACCGCC
GCAGTTGAAGGTTTCCCCATTAAGATCGCACTAATCAACAACGGAAACCTGGGCATGGTTCGCCAATGGCAGACCCTATT
CTATGAAGGACGGTACTCAAATACTAAACTTCGTAACCAGGGCGAGTACATGCCCGACTTTGTTGCCCTTTCTGAGGGAC
TTGGCTGTGTTGCCATCCGCGTCACCAAAGCGGAGGAAGTACTGCCAGCCATCCAAAAGGCTCGAGAAATCAACGACCGC
CCAGTAGTCATCGACTTCATCGTCGGTGAAGACGCACAGGTATGGCCAATGGTGTCTGCTGGATCATCCAACTCCGATAT
CCAGTACGCACTCGGATTGCGCCCATTCTTTGACGGCGACGAATCAGCTGCAGAAGACCCTGCAGACATTCATGCTTCCG
TTGATTCGACCGAGGCATAA

Upstream 100 bases:

>100_bases
ACAATTTTAAATGAGGGCTTTGTCTTTAGGCTGAGTTGAAATCGGCTTGGCTTGGACGGGTCCTGTGAAAATCCTTATTT
AGTAAAGGAGCCAGAAAGTC

Downstream 100 bases:

>100_bases
GGAGAGACCCAAGATGGCTAATTCTGACGTCACCCGCCACATCCTGTCCGTACTCGTTCAGGACGTAGACGGAATCATTT
CCCGCGTATCAGGTATGTTC

Product: acetolactate synthase 1 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MNVAASQQPTPATVASRGRSAAPERMTGAQAIVRSLEELNADIVFGIPGGAVLPVYDPLYSSTKVRHVLVRHEQGAGHAA
TGYAQVTGRVGVCIATSGPGATNLVTPIADANLDSVPMVAITGQVGSGLLGTDAFQEADIRGITMPVTKHNFMVTDPNDI
PQALAEAFHLAITGRPGPVLVDIPKDVQNAELDFVWPPKIDLPGYRPVSTPHARQIEQAVKLIGEAKKPVLYVGGGVIKA
DAHEELRAFAEYTGIPVVTTLMALGTFPESHELHMGMPGMHGTVSAVGALQRSDLLIAIGSRFDDRVTGDVDTFAPDAKI
IHADIDPAEIGKIKQVEVPIVGDAREVLARLLETTKASKAETEDISEWVDYLKGLKARFPRGYDEQPGDLLAPQFVIETL
SKEVGPDAIYCAGVGQHQMWAAQFVDFEKPRTWLNSGGLGTMGYAVPAALGAKAGAPDKEVWAIDGDGCFQMTNQELTTA
AVEGFPIKIALINNGNLGMVRQWQTLFYEGRYSNTKLRNQGEYMPDFVALSEGLGCVAIRVTKAEEVLPAIQKAREINDR
PVVIDFIVGEDAQVWPMVSAGSSNSDIQYALGLRPFFDGDESAAEDPADIHASVDSTEA

Sequences:

>Translated_619_residues
MNVAASQQPTPATVASRGRSAAPERMTGAQAIVRSLEELNADIVFGIPGGAVLPVYDPLYSSTKVRHVLVRHEQGAGHAA
TGYAQVTGRVGVCIATSGPGATNLVTPIADANLDSVPMVAITGQVGSGLLGTDAFQEADIRGITMPVTKHNFMVTDPNDI
PQALAEAFHLAITGRPGPVLVDIPKDVQNAELDFVWPPKIDLPGYRPVSTPHARQIEQAVKLIGEAKKPVLYVGGGVIKA
DAHEELRAFAEYTGIPVVTTLMALGTFPESHELHMGMPGMHGTVSAVGALQRSDLLIAIGSRFDDRVTGDVDTFAPDAKI
IHADIDPAEIGKIKQVEVPIVGDAREVLARLLETTKASKAETEDISEWVDYLKGLKARFPRGYDEQPGDLLAPQFVIETL
SKEVGPDAIYCAGVGQHQMWAAQFVDFEKPRTWLNSGGLGTMGYAVPAALGAKAGAPDKEVWAIDGDGCFQMTNQELTTA
AVEGFPIKIALINNGNLGMVRQWQTLFYEGRYSNTKLRNQGEYMPDFVALSEGLGCVAIRVTKAEEVLPAIQKAREINDR
PVVIDFIVGEDAQVWPMVSAGSSNSDIQYALGLRPFFDGDESAAEDPADIHASVDSTEA
>Mature_619_residues
MNVAASQQPTPATVASRGRSAAPERMTGAQAIVRSLEELNADIVFGIPGGAVLPVYDPLYSSTKVRHVLVRHEQGAGHAA
TGYAQVTGRVGVCIATSGPGATNLVTPIADANLDSVPMVAITGQVGSGLLGTDAFQEADIRGITMPVTKHNFMVTDPNDI
PQALAEAFHLAITGRPGPVLVDIPKDVQNAELDFVWPPKIDLPGYRPVSTPHARQIEQAVKLIGEAKKPVLYVGGGVIKA
DAHEELRAFAEYTGIPVVTTLMALGTFPESHELHMGMPGMHGTVSAVGALQRSDLLIAIGSRFDDRVTGDVDTFAPDAKI
IHADIDPAEIGKIKQVEVPIVGDAREVLARLLETTKASKAETEDISEWVDYLKGLKARFPRGYDEQPGDLLAPQFVIETL
SKEVGPDAIYCAGVGQHQMWAAQFVDFEKPRTWLNSGGLGTMGYAVPAALGAKAGAPDKEVWAIDGDGCFQMTNQELTTA
AVEGFPIKIALINNGNLGMVRQWQTLFYEGRYSNTKLRNQGEYMPDFVALSEGLGCVAIRVTKAEEVLPAIQKAREINDR
PVVIDFIVGEDAQVWPMVSAGSSNSDIQYALGLRPFFDGDESAAEDPADIHASVDSTEA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=484, Percent_Identity=26.2396694214876, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI21361361, Length=591, Percent_Identity=24.5346869712352, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI87081685, Length=560, Percent_Identity=45.7142857142857, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1790104, Length=577, Percent_Identity=44.5407279029463, Blast_Score=463, Evalue=1e-131,
Organism=Escherichia coli, GI1786717, Length=564, Percent_Identity=38.6524822695035, Blast_Score=367, Evalue=1e-102,
Organism=Escherichia coli, GI1787096, Length=565, Percent_Identity=29.9115044247788, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1788716, Length=555, Percent_Identity=27.7477477477477, Blast_Score=178, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17531299, Length=577, Percent_Identity=26.3431542461005, Blast_Score=148, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=577, Percent_Identity=26.3431542461005, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=511, Percent_Identity=26.8101761252446, Blast_Score=135, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=609, Percent_Identity=44.0065681444992, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6320816, Length=576, Percent_Identity=23.9583333333333, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6323163, Length=494, Percent_Identity=24.8987854251012, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19922626, Length=554, Percent_Identity=25.6317689530686, Blast_Score=166, Evalue=7e-41,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 66090; Mature: 66090

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVAASQQPTPATVASRGRSAAPERMTGAQAIVRSLEELNADIVFGIPGGAVLPVYDPLY
CCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCC
SSTKVRHVLVRHEQGAGHAATGYAQVTGRVGVCIATSGPGATNLVTPIADANLDSVPMVA
CCCCEEEEEEEECCCCCCCCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEE
ITGQVGSGLLGTDAFQEADIRGITMPVTKHNFMVTDPNDIPQALAEAFHLAITGRPGPVL
EECCCCCCCCCCCCHHCCCCCEEEEEEECCCEEEECCCHHHHHHHHHHEEEEECCCCCEE
VDIPKDVQNAELDFVWPPKIDLPGYRPVSTPHARQIEQAVKLIGEAKKPVLYVGGGVIKA
EECCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCEEEC
DAHEELRAFAEYTGIPVVTTLMALGTFPESHELHMGMPGMHGTVSAVGALQRSDLLIAIG
CCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHHHCCEEEEEC
SRFDDRVTGDVDTFAPDAKIIHADIDPAEIGKIKQVEVPIVGDAREVLARLLETTKASKA
CCCCCCCCCCHHHCCCCCEEEEECCCHHHCCCEEEEEEEECCCHHHHHHHHHHHHCCCCC
ETEDISEWVDYLKGLKARFPRGYDEQPGDLLAPQFVIETLSKEVGPDAIYCAGVGQHQMW
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHH
AAQFVDFEKPRTWLNSGGLGTMGYAVPAALGAKAGAPDKEVWAIDGDGCFQMTNQELTTA
HHHHHCCCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEECCCHHHHH
AVEGFPIKIALINNGNLGMVRQWQTLFYEGRYSNTKLRNQGEYMPDFVALSEGLGCVAIR
EECCCEEEEEEEECCCCCHHHHEEHHEECCCCCCCEECCCCCCCCHHHHHHCCCCEEEEE
VTKAEEVLPAIQKAREINDRPVVIDFIVGEDAQVWPMVSAGSSNSDIQYALGLRPFFDGD
EECHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCEEEEECCCCCCCCC
ESAAEDPADIHASVDSTEA
CCCCCCCCCEEECCCCCCC
>Mature Secondary Structure
MNVAASQQPTPATVASRGRSAAPERMTGAQAIVRSLEELNADIVFGIPGGAVLPVYDPLY
CCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCC
SSTKVRHVLVRHEQGAGHAATGYAQVTGRVGVCIATSGPGATNLVTPIADANLDSVPMVA
CCCCEEEEEEEECCCCCCCCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEE
ITGQVGSGLLGTDAFQEADIRGITMPVTKHNFMVTDPNDIPQALAEAFHLAITGRPGPVL
EECCCCCCCCCCCCHHCCCCCEEEEEEECCCEEEECCCHHHHHHHHHHEEEEECCCCCEE
VDIPKDVQNAELDFVWPPKIDLPGYRPVSTPHARQIEQAVKLIGEAKKPVLYVGGGVIKA
EECCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCEEEC
DAHEELRAFAEYTGIPVVTTLMALGTFPESHELHMGMPGMHGTVSAVGALQRSDLLIAIG
CCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHHHCCEEEEEC
SRFDDRVTGDVDTFAPDAKIIHADIDPAEIGKIKQVEVPIVGDAREVLARLLETTKASKA
CCCCCCCCCCHHHCCCCCEEEEECCCHHHCCCEEEEEEEECCCHHHHHHHHHHHHCCCCC
ETEDISEWVDYLKGLKARFPRGYDEQPGDLLAPQFVIETLSKEVGPDAIYCAGVGQHQMW
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHH
AAQFVDFEKPRTWLNSGGLGTMGYAVPAALGAKAGAPDKEVWAIDGDGCFQMTNQELTTA
HHHHHCCCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEECCCHHHHH
AVEGFPIKIALINNGNLGMVRQWQTLFYEGRYSNTKLRNQGEYMPDFVALSEGLGCVAIR
EECCCEEEEEEEECCCCCHHHHEEHHEECCCCCCCEECCCCCCCCHHHHHHCCCCEEEEE
VTKAEEVLPAIQKAREINDRPVVIDFIVGEDAQVWPMVSAGSSNSDIQYALGLRPFFDGD
EECHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCEEEEECCCCCCCCC
ESAAEDPADIHASVDSTEA
CCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8366043; 12948626 [H]