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The map label for this gene is gatA [H]

Identifier: 145295386

GI number: 145295386

Start: 1454416

End: 1455909

Strand: Direct

Name: gatA [H]

Synonym: cgR_1324

Alternate gene names: 145295386

Gene position: 1454416-1455909 (Clockwise)

Preceding gene: 145295385

Following gene: 145295387

Centisome position: 43.88

GC content: 58.37

Gene sequence:

>1494_bases
ATGACCAACAAGTACCTGGTTGAAGGCTCTGAAAACGAGCTGACCACAAAGACCGCAGCAGAGCTGGCAGGTCTTATTCA
TTCCCGCGAGGTAACTTCCCGCGAGGTTACTCAAGCGCACCTAGATCGCATTGCTGCGGTTGACGGCGATATTCATGCAT
TTCTCCACGTTGGCCAGGAGGAGGCCCTGAACGCGGCGGATGACGTCGATAAGCGTCTAGACGCTGGAGAGGCACCTGCC
TCGGCTTTGGCTGGCGTGCCGCTTGCACTGAAGGATGTCTTTACCACCACTGATGCGCCGACCACGGCGGCATCGAAGAT
GCTTGAGGGCTACATGAGCCCTTATGACGCGACTGTGACCCGCAAGATCCGTGAGGCTGGCATCCCAATTTTGGGTAAGA
CCAACATGGATGAGTTTGCGATGGGTTCCTCCACTGAGAACTCCGCATACGGCCCAACCCACAATCCGTGGGATCTGGAG
CGCACCGCAGGTGGTTCTGGTGGTGGCTCTTCGGCTGCTCTTGCTGCAGGTCAGGCGCCACTTGCGATTGGTACTGACAC
TGGTGGATCCATCCGTCAGCCAGCTGCGCTGACCAACACTGTTGGTGTGAAGCCAACCTACGGAACCGTATCCCGTTACG
GTCTGATTGCGTGTGCGTCCTCCCTGGATCAGGGTGGCCCAACCGCTCGTACTGTTCTGGATACCGCGCTTTTGCACGAG
GTTATCGCAGGCCACGACGGTTTTGATGCGACCTCCGTGAATCGTCCGGTTGCTCCTGTTGTGCAGGCTGCCCGTGAAGG
CGCGAACGGTGATCTGAAGGGCATGAAGGTCGGTGTGGTCAAGCAGTTCGACCGCGACGGCTACCAGCCTGGCGTGCTTG
AGGCATTCCACGCTTCTGTTGAGCAGATGCGCTCCCAGGGTGCGGAAATCGTTGAGGTTGATTGCCCTCACTTTGATGAC
GCTCTTGGCGCGTACTACCTGATTCTTCCTTGTGAAGTTTCCTCCAACCTCGCGCGTTTTGACGGCATGCGTTACGGTTT
GCGCGCCGGTGATGACGGAACTCGTTCCGCCGATGAGGTCATGGCATACACCCGTGCGCAGGGATTCGGCCCTGAGGTTA
AGCGCCGTATCATCCTCGGCACCTACGCGTTGTCTGTTGGTTACTACGACGCGTACTACCTGCAGGCACAGCGCGTGCGC
ACCCTTATTGCGCAGGACTTCGCGAAGGCTTACGAGCAGGTCGATATCCTGGTGTCCCCAACCACTCCAACCACCGCGTT
CAAGCTGGGGGAGAAGGTCACCGATCCGCTGGAGATGTACAACTTCGACTTGTGCACCCTGCCACTGAACCTGGCTGGTC
TCGCGGGCATGTCCCTGCCTTCCGGCTTGGCATCAGATACTGGGCTGCCTGTTGGTTTGCAGCTGATGGCTCCTGCTTTC
CAGGACGATCGTCTGTACCGCGTCGGCGCTGCTTTTGAAGCTGGACGCAAGTAG

Upstream 100 bases:

>100_bases
ACAAGACCCTCGATGCTGCGGCTGCGTTGGACCAAGCGCCCGCTGTCGAGGATGGACGTTTTATGGTTCCGCAGATTCTG
GGTGAGGGCGACTAATAATT

Downstream 100 bases:

>100_bases
GTTCTAAACCCTTTTTAAGAAATTGGCTGCCCTCGCATGACTGTGCGCGGGCGGCCAATTTTTTCTTTTGTGGGGGTGGC
TCTTACGCATTTCCTGAATT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MTNKYLVEGSENELTTKTAAELAGLIHSREVTSREVTQAHLDRIAAVDGDIHAFLHVGQEEALNAADDVDKRLDAGEAPA
SALAGVPLALKDVFTTTDAPTTAASKMLEGYMSPYDATVTRKIREAGIPILGKTNMDEFAMGSSTENSAYGPTHNPWDLE
RTAGGSGGGSSAALAAGQAPLAIGTDTGGSIRQPAALTNTVGVKPTYGTVSRYGLIACASSLDQGGPTARTVLDTALLHE
VIAGHDGFDATSVNRPVAPVVQAAREGANGDLKGMKVGVVKQFDRDGYQPGVLEAFHASVEQMRSQGAEIVEVDCPHFDD
ALGAYYLILPCEVSSNLARFDGMRYGLRAGDDGTRSADEVMAYTRAQGFGPEVKRRIILGTYALSVGYYDAYYLQAQRVR
TLIAQDFAKAYEQVDILVSPTTPTTAFKLGEKVTDPLEMYNFDLCTLPLNLAGLAGMSLPSGLASDTGLPVGLQLMAPAF
QDDRLYRVGAAFEAGRK

Sequences:

>Translated_497_residues
MTNKYLVEGSENELTTKTAAELAGLIHSREVTSREVTQAHLDRIAAVDGDIHAFLHVGQEEALNAADDVDKRLDAGEAPA
SALAGVPLALKDVFTTTDAPTTAASKMLEGYMSPYDATVTRKIREAGIPILGKTNMDEFAMGSSTENSAYGPTHNPWDLE
RTAGGSGGGSSAALAAGQAPLAIGTDTGGSIRQPAALTNTVGVKPTYGTVSRYGLIACASSLDQGGPTARTVLDTALLHE
VIAGHDGFDATSVNRPVAPVVQAAREGANGDLKGMKVGVVKQFDRDGYQPGVLEAFHASVEQMRSQGAEIVEVDCPHFDD
ALGAYYLILPCEVSSNLARFDGMRYGLRAGDDGTRSADEVMAYTRAQGFGPEVKRRIILGTYALSVGYYDAYYLQAQRVR
TLIAQDFAKAYEQVDILVSPTTPTTAFKLGEKVTDPLEMYNFDLCTLPLNLAGLAGMSLPSGLASDTGLPVGLQLMAPAF
QDDRLYRVGAAFEAGRK
>Mature_496_residues
TNKYLVEGSENELTTKTAAELAGLIHSREVTSREVTQAHLDRIAAVDGDIHAFLHVGQEEALNAADDVDKRLDAGEAPAS
ALAGVPLALKDVFTTTDAPTTAASKMLEGYMSPYDATVTRKIREAGIPILGKTNMDEFAMGSSTENSAYGPTHNPWDLER
TAGGSGGGSSAALAAGQAPLAIGTDTGGSIRQPAALTNTVGVKPTYGTVSRYGLIACASSLDQGGPTARTVLDTALLHEV
IAGHDGFDATSVNRPVAPVVQAAREGANGDLKGMKVGVVKQFDRDGYQPGVLEAFHASVEQMRSQGAEIVEVDCPHFDDA
LGAYYLILPCEVSSNLARFDGMRYGLRAGDDGTRSADEVMAYTRAQGFGPEVKRRIILGTYALSVGYYDAYYLQAQRVRT
LIAQDFAKAYEQVDILVSPTTPTTAFKLGEKVTDPLEMYNFDLCTLPLNLAGLAGMSLPSGLASDTGLPVGLQLMAPAFQ
DDRLYRVGAAFEAGRK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=34.9301397205589, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI195972892, Length=512, Percent_Identity=25.9765625, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI166795287, Length=218, Percent_Identity=29.8165137614679, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=423, Percent_Identity=36.8794326241135, Blast_Score=242, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI17537465, Length=523, Percent_Identity=23.9005736137667, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=309, Percent_Identity=25.5663430420712, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=308, Percent_Identity=24.025974025974, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=331, Percent_Identity=26.5861027190332, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=315, Percent_Identity=27.6190476190476, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=315, Percent_Identity=27.6190476190476, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=414, Percent_Identity=34.5410628019324, Blast_Score=222, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6319685, Length=430, Percent_Identity=29.7674418604651, Blast_Score=116, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6320448, Length=207, Percent_Identity=29.4685990338164, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648113, Length=458, Percent_Identity=41.4847161572052, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24644968, Length=516, Percent_Identity=25.5813953488372, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI21356731, Length=244, Percent_Identity=32.7868852459016, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24652985, Length=498, Percent_Identity=24.8995983935743, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19922090, Length=498, Percent_Identity=24.8995983935743, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24652981, Length=498, Percent_Identity=24.8995983935743, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24652983, Length=498, Percent_Identity=24.8995983935743, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI45550774, Length=251, Percent_Identity=31.4741035856574, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24648435, Length=251, Percent_Identity=31.4741035856574, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24648437, Length=251, Percent_Identity=31.4741035856574, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI161078093, Length=524, Percent_Identity=25.9541984732824, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24648441, Length=183, Percent_Identity=31.6939890710383, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648439, Length=183, Percent_Identity=31.6939890710383, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52555; Mature: 52423

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKYLVEGSENELTTKTAAELAGLIHSREVTSREVTQAHLDRIAAVDGDIHAFLHVGQE
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHH
EALNAADDVDKRLDAGEAPASALAGVPLALKDVFTTTDAPTTAASKMLEGYMSPYDATVT
HHCCCHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHH
RKIREAGIPILGKTNMDEFAMGSSTENSAYGPTHNPWDLERTAGGSGGGSSAALAAGQAP
HHHHHCCCCEEECCCCHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCEEECCCCC
LAIGTDTGGSIRQPAALTNTVGVKPTYGTVSRYGLIACASSLDQGGPTARTVLDTALLHE
EEEECCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VIAGHDGFDATSVNRPVAPVVQAAREGANGDLKGMKVGVVKQFDRDGYQPGVLEAFHASV
HHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHCCCCCCCCHHHHHHHHH
EQMRSQGAEIVEVDCPHFDDALGAYYLILPCEVSSNLARFDGMRYGLRAGDDGTRSADEV
HHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCHHHH
MAYTRAQGFGPEVKRRIILGTYALSVGYYDAYYLQAQRVRTLIAQDFAKAYEQVDILVSP
HHHHHHCCCCHHHHHEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEC
TTPTTAFKLGEKVTDPLEMYNFDLCTLPLNLAGLAGMSLPSGLASDTGLPVGLQLMAPAF
CCCCHHHHHCHHCCCHHHHHCCCEEECCCHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCC
QDDRLYRVGAAFEAGRK
CCCCEEEECCHHHCCCC
>Mature Secondary Structure 
TNKYLVEGSENELTTKTAAELAGLIHSREVTSREVTQAHLDRIAAVDGDIHAFLHVGQE
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHH
EALNAADDVDKRLDAGEAPASALAGVPLALKDVFTTTDAPTTAASKMLEGYMSPYDATVT
HHCCCHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHH
RKIREAGIPILGKTNMDEFAMGSSTENSAYGPTHNPWDLERTAGGSGGGSSAALAAGQAP
HHHHHCCCCEEECCCCHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCEEECCCCC
LAIGTDTGGSIRQPAALTNTVGVKPTYGTVSRYGLIACASSLDQGGPTARTVLDTALLHE
EEEECCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VIAGHDGFDATSVNRPVAPVVQAAREGANGDLKGMKVGVVKQFDRDGYQPGVLEAFHASV
HHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHCCCCCCCCHHHHHHHHH
EQMRSQGAEIVEVDCPHFDDALGAYYLILPCEVSSNLARFDGMRYGLRAGDDGTRSADEV
HHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCHHHH
MAYTRAQGFGPEVKRRIILGTYALSVGYYDAYYLQAQRVRTLIAQDFAKAYEQVDILVSP
HHHHHHCCCCHHHHHEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEC
TTPTTAFKLGEKVTDPLEMYNFDLCTLPLNLAGLAGMSLPSGLASDTGLPVGLQLMAPAF
CCCCHHHHHCHHCCCHHHHHCCCEEECCCHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCC
QDDRLYRVGAAFEAGRK
CCCCEEEECCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]