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The map label for this gene is speE [C]

Identifier: 145295376

GI number: 145295376

Start: 1443775

End: 1444719

Strand: Reverse

Name: speE [C]

Synonym: cgR_1314

Alternate gene names: 145295376

Gene position: 1444719-1443775 (Counterclockwise)

Preceding gene: 145295377

Following gene: 145295374

Centisome position: 43.59

GC content: 56.93

Gene sequence:

>945_bases
GTGGCCCGTAAGAAAAACACGTCCGATCAATCCCGCTCCCAAGCTGCCAACACTCCCATTGCTGGCACCTATGAGGGTGA
ATATTCCGTCATCGAGTTGGAGGCCGATTCCTACACCACCGATGGCTGGTTGATCAGCATTAATGGCGTGCCCAGTTCTC
ATATTGTCCTGGGTCAACCGCAGGCACTGGAATTTGAGTACATGCGGTGGATCGCTACCGGCGCTCGGGCGTTCATCGAT
GCGCATCAGGATGCATCCAAGCTGCGGATTACTCACCTCGGCGGCGGTGCGTGCACGATGGCCAGGTATTTCGCGGATGT
TTACCCGCAGTCACGCAACACTGTCGTGGAATTGGATGCAGAGCTTGCCCGCCTGTCACGTGAATGGTTCGACATTCCGC
GCGCGCCACGGGTAAAGATTCGTGTGGATGATGCCCGAATGGTTGCAGAATCTTTTACTCCCGCAAGCCGCGATGTGATC
ATCCGTGATGTATTCGCCGGAGCCGTCACTCCGCAAAACTTCACCACCGTGGAGTTCTTTGAGCACTGCCACCGAGGCCT
TGCTCCTGGCGGATTGTACATTGCCAACTGTGGCGACCATTCCGATCTGCGCGGAGCTAAATCTGAGCTCGCCGGCATGA
TGGAGGTGTTCGAGCACGTCGCAGTCATCGCCGACCCCCCGATGCTTAAGGGGCGCCGTTACGGCAACATCATTTTGATG
GGTTCAGACACCGAATTCTTTAGATCCAACAGCACGGAAGCGTCCGCGATTACCCGTGAGCTTCTTGGCGGCGGCGTTCC
AGCGCAGTACAAGGATGAATCCTGGGTGCGGAAATTCGCCTCGGGAGCCCAGGCCCGCCACGATGGGGTCTCTACCCTCC
AAATGCCGAGTGATACTTCACAACACCCTGCGGAAACGCCGGAGCATTCAAACACACAGCCATAA

Upstream 100 bases:

>100_bases
TTTGCCGTACATGCGCGAGCACCTCCTCAACAGCCCGCACCACCGACCAATCACATAAGACATAAGCACTAAAACAGCAT
TAAAGAAAGAAAGCTTTTTC

Downstream 100 bases:

>100_bases
AAAATTCCGCTGGCGCGTCCGGATAGACCAGCCCGGGCACGACCTCAAAACCGAAGCGTCGATAATAGCCCGGATCCCCC
AGCAACACGACGCCGCCAGC

Product: hypothetical protein

Products: 5-Methylthioadenosine; H+; Spermidine [C]

Alternate protein names: Spermidine Synthase-Like Protein; Spermine Synthase; Methyltransferase Type; Transferase; Sam Dependent Methyltransferase

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MARKKNTSDQSRSQAANTPIAGTYEGEYSVIELEADSYTTDGWLISINGVPSSHIVLGQPQALEFEYMRWIATGARAFID
AHQDASKLRITHLGGGACTMARYFADVYPQSRNTVVELDAELARLSREWFDIPRAPRVKIRVDDARMVAESFTPASRDVI
IRDVFAGAVTPQNFTTVEFFEHCHRGLAPGGLYIANCGDHSDLRGAKSELAGMMEVFEHVAVIADPPMLKGRRYGNIILM
GSDTEFFRSNSTEASAITRELLGGGVPAQYKDESWVRKFASGAQARHDGVSTLQMPSDTSQHPAETPEHSNTQP

Sequences:

>Translated_314_residues
MARKKNTSDQSRSQAANTPIAGTYEGEYSVIELEADSYTTDGWLISINGVPSSHIVLGQPQALEFEYMRWIATGARAFID
AHQDASKLRITHLGGGACTMARYFADVYPQSRNTVVELDAELARLSREWFDIPRAPRVKIRVDDARMVAESFTPASRDVI
IRDVFAGAVTPQNFTTVEFFEHCHRGLAPGGLYIANCGDHSDLRGAKSELAGMMEVFEHVAVIADPPMLKGRRYGNIILM
GSDTEFFRSNSTEASAITRELLGGGVPAQYKDESWVRKFASGAQARHDGVSTLQMPSDTSQHPAETPEHSNTQP
>Mature_313_residues
ARKKNTSDQSRSQAANTPIAGTYEGEYSVIELEADSYTTDGWLISINGVPSSHIVLGQPQALEFEYMRWIATGARAFIDA
HQDASKLRITHLGGGACTMARYFADVYPQSRNTVVELDAELARLSREWFDIPRAPRVKIRVDDARMVAESFTPASRDVII
RDVFAGAVTPQNFTTVEFFEHCHRGLAPGGLYIANCGDHSDLRGAKSELAGMMEVFEHVAVIADPPMLKGRRYGNIILMG
SDTEFFRSNSTEASAITRELLGGGVPAQYKDESWVRKFASGAQARHDGVSTLQMPSDTSQHPAETPEHSNTQP

Specific function: Spermidine biosynthesis from arginine; third (last) step. [C]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.5.1.16 [C]

Molecular weight: Translated: 34541; Mature: 34410

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKKNTSDQSRSQAANTPIAGTYEGEYSVIELEADSYTTDGWLISINGVPSSHIVLGQP
CCCCCCCCHHHHHHHCCCCCCEEECCCEEEEEEECCCCCCCCEEEEECCCCCCCEEECCC
QALEFEYMRWIATGARAFIDAHQDASKLRITHLGGGACTMARYFADVYPQSRNTVVELDA
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECH
ELARLSREWFDIPRAPRVKIRVDDARMVAESFTPASRDVIIRDVFAGAVTPQNFTTVEFF
HHHHHHHHHHCCCCCCEEEEEECHHHHHHHHCCCCCCCEEHHHHHHCCCCCCCCHHHHHH
EHCHRGLAPGGLYIANCGDHSDLRGAKSELAGMMEVFEHVAVIADPPMLKGRRYGNIILM
HHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
GSDTEFFRSNSTEASAITRELLGGGVPAQYKDESWVRKFASGAQARHDGVSTLQMPSDTS
CCCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHCCCCEEECCCCCC
QHPAETPEHSNTQP
CCCCCCCCCCCCCC
>Mature Secondary Structure 
ARKKNTSDQSRSQAANTPIAGTYEGEYSVIELEADSYTTDGWLISINGVPSSHIVLGQP
CCCCCCCHHHHHHHCCCCCCEEECCCEEEEEEECCCCCCCCEEEEECCCCCCCEEECCC
QALEFEYMRWIATGARAFIDAHQDASKLRITHLGGGACTMARYFADVYPQSRNTVVELDA
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECH
ELARLSREWFDIPRAPRVKIRVDDARMVAESFTPASRDVIIRDVFAGAVTPQNFTTVEFF
HHHHHHHHHHCCCCCCEEEEEECHHHHHHHHCCCCCCCEEHHHHHHCCCCCCCCHHHHHH
EHCHRGLAPGGLYIANCGDHSDLRGAKSELAGMMEVFEHVAVIADPPMLKGRRYGNIILM
HHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
GSDTEFFRSNSTEASAITRELLGGGVPAQYKDESWVRKFASGAQARHDGVSTLQMPSDTS
CCCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHCCCCEEECCCCCC
QHPAETPEHSNTQP
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.09 {1,4-diaminobutane}} 0.012 {1,4-diaminobutane}} 0.0022 {S-adenosyl-3-methylthio-1-propylamine}} [C]

Substrates: S-Adenosylmethioninamine; Putrescine [C]

Specific reaction: S-Adenosylmethioninamine + Putrescine --> 5-Methylthioadenosine + H+ + Spermidine [C]

General reaction: Aminopropyl group transfer [C]

Inhibitor: 5'-Ethyl thioadenosine; 5'-Methyl thioadenosine; Dicyclohexyl amine; carbonyl binding reagents, [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA