Click here to switch to the map view.

The map label for this gene is alkK [H]

Identifier: 145295337

GI number: 145295337

Start: 1405658

End: 1407415

Strand: Reverse

Name: alkK [H]

Synonym: cgR_1277

Alternate gene names: 145295337

Gene position: 1407415-1405658 (Counterclockwise)

Preceding gene: 145295344

Following gene: 145295336

Centisome position: 42.47

GC content: 55.46

Gene sequence:

>1758_bases
GTGTCGCATGCCCCGGCTATTGTTGATTGCATGCTCAGCACGATGCAGGACGTCCCACTGTCATTAACCAGAATCCTCGA
GTACGGGTCCACTGTTCACGGTGATACTTTGATCACCACGTGGGGCGGTGCCGATGACATTGAACAAGCACAGCAAACTT
TTAGTGCTGTGGGGGCTAGAGCTGCGGCTTTGGCTCATGCACTGCATGATTCTTTAGGTATTACTGGGGATCAGCGAGTG
GCGTCGATGCTCTATAACTGCGCGGAACACATGGAAACTATGTTTGCAGTCGCATGCATGGGTGCCGTGTTTAATCCGCT
GAACAAGCAGTTGATGAATGATCAGATTGTGTTCATTCTCAATCACTCTGAAGCTGAAGTGGTTATCGCTGATCCGCGCA
TGGCTGAACAATTGGGTGAGATCTTAAAAGAAACACCAAAAGTTCGTGCCGTGGTGTTTATTGGACCGAATGATTTCTCT
AGCGCGGCGGCCCACATGCCGGAGGGAATGAAGCTGTATTCCTATGAAGCGCTCCTTGACGGCCGTTCCACTGTTTACAA
CTGGCCCGAGCAGGATGAACGCACTGCTGCCGCGATTTGCTATTCCACCGGTACATCGGGACCGCCGAAGGGTGTGGTGT
ATTCCCACCGCTCGCTTTATTTACAGTCGTTGAGCTTGCGCACCACGGATTCCCTCGCAGTGGAACACGGTGAAACGTTC
CTGTGCTGTGTGCCGATTTACCACGTCCTCAGCTGGGGCGTGCCGATCGCAGCGTTTATGTCCGGCACTCCCCTGGTGCT
TCCTGGACCGGATCTTTCTGCGCCGACATTGGCAAAGATCATTTCCACCACGCTGCCTCGCGTGGCGCATGGCGTGCCTA
CTCTGTGGATTCAGCTGATGGTTCACTACCTGAAAAATCCCCCAGAGCGTATGTCTCTGCGTGAGCTATACGTGGGCGGT
TCTGCGGTGCCTCCAATCGTGATCACCATGTGGGAGCAGCGCTATGGCGTGGATGTTGTCCACGTGTGGGGCATGACGGA
AACCTCCACCGTGGGTACTGTGTCTCGCCCACCATCAGGTGTTTCTGGTGAAACCAGGTGGAATTATCGAGTCTCCCAGG
GCCGATTCCCCGCCTCCCTGCAGTACCGCATTGTCAACGACGGCCAGGTCATGGCGTCCACTGACCGCAACGAGGGCGAG
ATTCAGGTCCGCGGTCCGTGGGTGACTGCAAGTTACTTCCACCCCGATGTGGAAAAAGAAGGTGGCACCGCCTCAACATT
CCGCGACCATGACGTTGAAGAGGAAAACGATGAGCTCTTCACCGCCGACGGCTGGCTGCGCACCGGGGACGTTGGATCTG
TCACCAGCGATGGATTCCTCACCATCCAAGACCGCGCCCGCGATGTCATCCGTTCTGGCGGCGAGTGGATTTACTCCGCT
CAGCTGGAAAACCTCATCGTGGCTACGGAAGAGGTTGTCGAATGCGCCGTCATTGGCTTCCCCGATGACAAGTGGGTGGA
ACGTCCCCTCGCAGTCACCATGCTCTACCCCGGCATTGAACGCACCCGGGAAACCGCCGAGCGCCTCCGCGATCAACTTC
GCGACCGCCTACCCAACTGGATGCTGCCAGAATATTGGACATTTGTTGATGAGGTGGATAAAACCTCCGTCGGTAAATAT
GACAAGAAGGACCTCCGCAACCACCTGCGCAACGGCGATTTCGAAGTAATTAAGCTTAAAGGGCCAGGTGAGAAATAA

Upstream 100 bases:

>100_bases
ATAATTCTAGCGCATGGTATTGCCCTATGTCTCATTCCCCTCGCTAAAAGCTGCATAAAGTTTCGACGTTTTCAAAGTTG
ATTGCTTGCACTGTCGTTGC

Downstream 100 bases:

>100_bases
TTTCCCTATTTATTCCGGCTTTTACTGGCTTTATTCCGCAGCTCTTTCTAGGATGGGCAAGAAGATGACTACTCGCCTTT
ATCTTTCGCCAACGCCGCCC

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MSHAPAIVDCMLSTMQDVPLSLTRILEYGSTVHGDTLITTWGGADDIEQAQQTFSAVGARAAALAHALHDSLGITGDQRV
ASMLYNCAEHMETMFAVACMGAVFNPLNKQLMNDQIVFILNHSEAEVVIADPRMAEQLGEILKETPKVRAVVFIGPNDFS
SAAAHMPEGMKLYSYEALLDGRSTVYNWPEQDERTAAAICYSTGTSGPPKGVVYSHRSLYLQSLSLRTTDSLAVEHGETF
LCCVPIYHVLSWGVPIAAFMSGTPLVLPGPDLSAPTLAKIISTTLPRVAHGVPTLWIQLMVHYLKNPPERMSLRELYVGG
SAVPPIVITMWEQRYGVDVVHVWGMTETSTVGTVSRPPSGVSGETRWNYRVSQGRFPASLQYRIVNDGQVMASTDRNEGE
IQVRGPWVTASYFHPDVEKEGGTASTFRDHDVEEENDELFTADGWLRTGDVGSVTSDGFLTIQDRARDVIRSGGEWIYSA
QLENLIVATEEVVECAVIGFPDDKWVERPLAVTMLYPGIERTRETAERLRDQLRDRLPNWMLPEYWTFVDEVDKTSVGKY
DKKDLRNHLRNGDFEVIKLKGPGEK

Sequences:

>Translated_585_residues
MSHAPAIVDCMLSTMQDVPLSLTRILEYGSTVHGDTLITTWGGADDIEQAQQTFSAVGARAAALAHALHDSLGITGDQRV
ASMLYNCAEHMETMFAVACMGAVFNPLNKQLMNDQIVFILNHSEAEVVIADPRMAEQLGEILKETPKVRAVVFIGPNDFS
SAAAHMPEGMKLYSYEALLDGRSTVYNWPEQDERTAAAICYSTGTSGPPKGVVYSHRSLYLQSLSLRTTDSLAVEHGETF
LCCVPIYHVLSWGVPIAAFMSGTPLVLPGPDLSAPTLAKIISTTLPRVAHGVPTLWIQLMVHYLKNPPERMSLRELYVGG
SAVPPIVITMWEQRYGVDVVHVWGMTETSTVGTVSRPPSGVSGETRWNYRVSQGRFPASLQYRIVNDGQVMASTDRNEGE
IQVRGPWVTASYFHPDVEKEGGTASTFRDHDVEEENDELFTADGWLRTGDVGSVTSDGFLTIQDRARDVIRSGGEWIYSA
QLENLIVATEEVVECAVIGFPDDKWVERPLAVTMLYPGIERTRETAERLRDQLRDRLPNWMLPEYWTFVDEVDKTSVGKY
DKKDLRNHLRNGDFEVIKLKGPGEK
>Mature_584_residues
SHAPAIVDCMLSTMQDVPLSLTRILEYGSTVHGDTLITTWGGADDIEQAQQTFSAVGARAAALAHALHDSLGITGDQRVA
SMLYNCAEHMETMFAVACMGAVFNPLNKQLMNDQIVFILNHSEAEVVIADPRMAEQLGEILKETPKVRAVVFIGPNDFSS
AAAHMPEGMKLYSYEALLDGRSTVYNWPEQDERTAAAICYSTGTSGPPKGVVYSHRSLYLQSLSLRTTDSLAVEHGETFL
CCVPIYHVLSWGVPIAAFMSGTPLVLPGPDLSAPTLAKIISTTLPRVAHGVPTLWIQLMVHYLKNPPERMSLRELYVGGS
AVPPIVITMWEQRYGVDVVHVWGMTETSTVGTVSRPPSGVSGETRWNYRVSQGRFPASLQYRIVNDGQVMASTDRNEGEI
QVRGPWVTASYFHPDVEKEGGTASTFRDHDVEEENDELFTADGWLRTGDVGSVTSDGFLTIQDRARDVIRSGGEWIYSAQ
LENLIVATEEVVECAVIGFPDDKWVERPLAVTMLYPGIERTRETAERLRDQLRDRLPNWMLPEYWTFVDEVDKTSVGKYD
KKDLRNHLRNGDFEVIKLKGPGEK

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=562, Percent_Identity=25.4448398576512, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI187761343, Length=562, Percent_Identity=25.4448398576512, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI156151445, Length=375, Percent_Identity=26.1333333333333, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI157311624, Length=525, Percent_Identity=22.8571428571429, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI157311622, Length=525, Percent_Identity=22.8571428571429, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI58082049, Length=525, Percent_Identity=22.2857142857143, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI122937307, Length=535, Percent_Identity=24.2990654205607, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI38505220, Length=526, Percent_Identity=23.9543726235741, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI115511026, Length=543, Percent_Identity=22.0994475138122, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI1788107, Length=548, Percent_Identity=24.8175182481752, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI221142682, Length=512, Percent_Identity=23.6328125, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI145693145, Length=541, Percent_Identity=23.8447319778189, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1786810, Length=380, Percent_Identity=25.2631578947368, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1789201, Length=321, Percent_Identity=23.6760124610592, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1790505, Length=559, Percent_Identity=22.7191413237925, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=521, Percent_Identity=26.295585412668, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17559526, Length=547, Percent_Identity=25.7769652650823, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI32563687, Length=523, Percent_Identity=21.6061185468451, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71994703, Length=551, Percent_Identity=23.5934664246824, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=551, Percent_Identity=23.5934664246824, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=551, Percent_Identity=23.5934664246824, Blast_Score=105, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17531443, Length=484, Percent_Identity=22.3140495867769, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17560308, Length=502, Percent_Identity=22.7091633466135, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=494, Percent_Identity=21.2550607287449, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17538037, Length=534, Percent_Identity=21.9101123595506, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17557194, Length=541, Percent_Identity=22.365988909427, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17541856, Length=472, Percent_Identity=21.6101694915254, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71985884, Length=370, Percent_Identity=22.4324324324324, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=492, Percent_Identity=24.7967479674797, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319264, Length=555, Percent_Identity=23.2432432432432, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24581924, Length=605, Percent_Identity=24.9586776859504, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21355181, Length=513, Percent_Identity=23.3918128654971, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI18859661, Length=534, Percent_Identity=22.4719101123595, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI19922652, Length=547, Percent_Identity=22.1206581352834, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 64813; Mature: 64682

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHAPAIVDCMLSTMQDVPLSLTRILEYGSTVHGDTLITTWGGADDIEQAQQTFSAVGAR
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
AAALAHALHDSLGITGDQRVASMLYNCAEHMETMFAVACMGAVFNPLNKQLMNDQIVFIL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
NHSEAEVVIADPRMAEQLGEILKETPKVRAVVFIGPNDFSSAAAHMPEGMKLYSYEALLD
ECCCCEEEEECCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCCCCEEEEEHHHHC
GRSTVYNWPEQDERTAAAICYSTGTSGPPKGVVYSHRSLYLQSLSLRTTDSLAVEHGETF
CCHHCCCCCCCCCCCEEEEEEECCCCCCCCCEEECHHHHHHHHHCCCCCCHHEEECCCEE
LCCVPIYHVLSWGVPIAAFMSGTPLVLPGPDLSAPTLAKIISTTLPRVAHGVPTLWIQLM
EEHHHHHHHHHCCCCHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHH
VHYLKNPPERMSLRELYVGGSAVPPIVITMWEQRYGVDVVHVWGMTETSTVGTVSRPPSG
HHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEHHHCCCEEEEEECCCCCCCCCCCCCCCCC
VSGETRWNYRVSQGRFPASLQYRIVNDGQVMASTDRNEGEIQVRGPWVTASYFHPDVEKE
CCCCCEEEEEECCCCCCCEEEEEEECCCEEEEECCCCCCEEEEECCEEEEECCCCCCCCC
GGTASTFRDHDVEEENDELFTADGWLRTGDVGSVTSDGFLTIQDRARDVIRSGGEWIYSA
CCCCCCCCCCCCCCCCCCEEEECCCEEECCCCCCCCCCEEEEHHHHHHHHHCCCCEEEEE
QLENLIVATEEVVECAVIGFPDDKWVERPLAVTMLYPGIERTRETAERLRDQLRDRLPNW
HHHHHHHHHHHHHEEEEECCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCC
MLPEYWTFVDEVDKTSVGKYDKKDLRNHLRNGDFEVIKLKGPGEK
CCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCC
>Mature Secondary Structure 
SHAPAIVDCMLSTMQDVPLSLTRILEYGSTVHGDTLITTWGGADDIEQAQQTFSAVGAR
CCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
AAALAHALHDSLGITGDQRVASMLYNCAEHMETMFAVACMGAVFNPLNKQLMNDQIVFIL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
NHSEAEVVIADPRMAEQLGEILKETPKVRAVVFIGPNDFSSAAAHMPEGMKLYSYEALLD
ECCCCEEEEECCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCCCCEEEEEHHHHC
GRSTVYNWPEQDERTAAAICYSTGTSGPPKGVVYSHRSLYLQSLSLRTTDSLAVEHGETF
CCHHCCCCCCCCCCCEEEEEEECCCCCCCCCEEECHHHHHHHHHCCCCCCHHEEECCCEE
LCCVPIYHVLSWGVPIAAFMSGTPLVLPGPDLSAPTLAKIISTTLPRVAHGVPTLWIQLM
EEHHHHHHHHHCCCCHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHH
VHYLKNPPERMSLRELYVGGSAVPPIVITMWEQRYGVDVVHVWGMTETSTVGTVSRPPSG
HHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEHHHCCCEEEEEECCCCCCCCCCCCCCCCC
VSGETRWNYRVSQGRFPASLQYRIVNDGQVMASTDRNEGEIQVRGPWVTASYFHPDVEKE
CCCCCEEEEEECCCCCCCEEEEEEECCCEEEEECCCCCCEEEEECCEEEEECCCCCCCCC
GGTASTFRDHDVEEENDELFTADGWLRTGDVGSVTSDGFLTIQDRARDVIRSGGEWIYSA
CCCCCCCCCCCCCCCCCCEEEECCCEEECCCCCCCCCCEEEEHHHHHHHHHCCCCEEEEE
QLENLIVATEEVVECAVIGFPDDKWVERPLAVTMLYPGIERTRETAERLRDQLRDRLPNW
HHHHHHHHHHHHHEEEEECCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCC
MLPEYWTFVDEVDKTSVGKYDKKDLRNHLRNGDFEVIKLKGPGEK
CCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]