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The map label for this gene is narK [H]

Identifier: 145295329

GI number: 145295329

Start: 1395329

End: 1396681

Strand: Reverse

Name: narK [H]

Synonym: cgR_1269

Alternate gene names: 145295329

Gene position: 1396681-1395329 (Counterclockwise)

Preceding gene: 145295331

Following gene: 145295328

Centisome position: 42.14

GC content: 52.62

Gene sequence:

>1353_bases
ATGAAGAGGCATCACGTGACACAACTCAACACCAAAGGCGTTGTTCTGCAAGGGTGGGATCCAGAAGATCCTGAACATTG
GGACTCGAAAATTGCATGGCGAACCCTGTGGATTACCACCTTCTCCATGATTATTGGGTTCTGCGTGTGGTATTTGGTTT
CTGCCATCGCTCCCCTACTCAATCGAATTGGATTTGATCTCTCAGCAAGTCAGCTTTATTGGCTCGCATCTATCCCCGGT
TTGGCCGGCGGATTAATCCGATTGATTTACATGTTCCTTCCACCGATTCTTGGAACCCGCAAATTGGTCGGAATTTCCTC
CGCTCTATTTTTGATCCCCATGTTTGGGTGGTTCCTTGCTGTCCAAGATTCAAGCACTCCCTACTGGTGGCTTCTCATAC
TCGCTGCACTCACTGGCATTGGTGGTGGCGTGTTCTCTGGATATATGCCGTCCACGGGATACTTCTTCCCCAAGGCAAAA
TCCGGTACTGCGCTGGGCATTCAGGCAGGTATCGGCAACCTCGGCGTCTCGATAATTCAGTTCATGGGTCCCTGGGTCAT
GGGTTTTGGTCTGCTGGGCATCGGATTCCTCACCCCACAACGCACCATCGAAGGCTCCACAGTGTTTGTGCACAATGCTG
CGATTGTGTTGGTCCCGTGGACTATTCTCGCGGCCGTTTTATCCTTCCTGTTTCTTAAAGATGTCCCAGTCACCGCAAAT
TTCCGGCAACAGATCGATATCTTTGGCAACAAGAACACATGGATTTTGTCCATCATCTATCTGATGACATTCGGTGCCTT
CGCGGGCTTCGCCGCACAGTTTGGCCTGATCATCAACAACAACTTCGGCATCGCTTCCCCGATGGCAGAGACTTATCCAG
CTGAAATGCTCCACGCTGGTGCAACGTTCGCGTTTCTTGGACCTTTGATTGGTGCTTTGGTGCGTGCTGCATGGGGTCCG
CTGTGTGACAGATTCGGTGGAGCTATCTGGACCTTTGTCGGTGGCATCGGAATGACTATCGCCACTGCAGCTGCCGCAAT
CTTCCTAAGCAGAGCGGAGACACCTGATGATTTCTGGCCATTCCTGTGGTCCATGCTTGCCCTGTTCTTCTTCACCGGTC
TGGGCAACGCCGGCACATTCAAACAAATGCCCATGATTTTGCCTAAACGCCAAGCAGGTGGCGTGATCGGCTGGACCGGT
GCCATTGGTGCCTTCGGCCCATTCATCGTCGGTGTCTTGCTCTCCTTCACTCCAACTGTCGCGTTCTTCTGGGGCTGCGT
GGTGTTCTTCATCATCGCCACCGCTTTGACCTGGATCTACTACGCCCGCCCGAACGCTCCATTCCCGGGATAA

Upstream 100 bases:

>100_bases
AAAACCCCGTTGAAAAACATGCGGGCGCAGGTCAGAGCTGTTATCTTAGTACTTATCACAGCCATAGGGCGGGCTTGACG
GAAAGCCTTTCCGCGTAACC

Downstream 100 bases:

>100_bases
ACCGAAAGGCCAATCCATGACTACAACTACTTCTTCTGGGAAGTCTTCTGAACAGTCTTCTGAAAAGATCAACCCCCTCT
TCAAGCTCGGCAGTTTCCTA

Product: hypothetical protein

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKRHHVTQLNTKGVVLQGWDPEDPEHWDSKIAWRTLWITTFSMIIGFCVWYLVSAIAPLLNRIGFDLSASQLYWLASIPG
LAGGLIRLIYMFLPPILGTRKLVGISSALFLIPMFGWFLAVQDSSTPYWWLLILAALTGIGGGVFSGYMPSTGYFFPKAK
SGTALGIQAGIGNLGVSIIQFMGPWVMGFGLLGIGFLTPQRTIEGSTVFVHNAAIVLVPWTILAAVLSFLFLKDVPVTAN
FRQQIDIFGNKNTWILSIIYLMTFGAFAGFAAQFGLIINNNFGIASPMAETYPAEMLHAGATFAFLGPLIGALVRAAWGP
LCDRFGGAIWTFVGGIGMTIATAAAAIFLSRAETPDDFWPFLWSMLALFFFTGLGNAGTFKQMPMILPKRQAGGVIGWTG
AIGAFGPFIVGVLLSFTPTVAFFWGCVVFFIIATALTWIYYARPNAPFPG

Sequences:

>Translated_450_residues
MKRHHVTQLNTKGVVLQGWDPEDPEHWDSKIAWRTLWITTFSMIIGFCVWYLVSAIAPLLNRIGFDLSASQLYWLASIPG
LAGGLIRLIYMFLPPILGTRKLVGISSALFLIPMFGWFLAVQDSSTPYWWLLILAALTGIGGGVFSGYMPSTGYFFPKAK
SGTALGIQAGIGNLGVSIIQFMGPWVMGFGLLGIGFLTPQRTIEGSTVFVHNAAIVLVPWTILAAVLSFLFLKDVPVTAN
FRQQIDIFGNKNTWILSIIYLMTFGAFAGFAAQFGLIINNNFGIASPMAETYPAEMLHAGATFAFLGPLIGALVRAAWGP
LCDRFGGAIWTFVGGIGMTIATAAAAIFLSRAETPDDFWPFLWSMLALFFFTGLGNAGTFKQMPMILPKRQAGGVIGWTG
AIGAFGPFIVGVLLSFTPTVAFFWGCVVFFIIATALTWIYYARPNAPFPG
>Mature_450_residues
MKRHHVTQLNTKGVVLQGWDPEDPEHWDSKIAWRTLWITTFSMIIGFCVWYLVSAIAPLLNRIGFDLSASQLYWLASIPG
LAGGLIRLIYMFLPPILGTRKLVGISSALFLIPMFGWFLAVQDSSTPYWWLLILAALTGIGGGVFSGYMPSTGYFFPKAK
SGTALGIQAGIGNLGVSIIQFMGPWVMGFGLLGIGFLTPQRTIEGSTVFVHNAAIVLVPWTILAAVLSFLFLKDVPVTAN
FRQQIDIFGNKNTWILSIIYLMTFGAFAGFAAQFGLIINNNFGIASPMAETYPAEMLHAGATFAFLGPLIGALVRAAWGP
LCDRFGGAIWTFVGGIGMTIATAAAAIFLSRAETPDDFWPFLWSMLALFFFTGLGNAGTFKQMPMILPKRQAGGVIGWTG
AIGAFGPFIVGVLLSFTPTVAFFWGCVVFFIIATALTWIYYARPNAPFPG

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=472, Percent_Identity=31.9915254237288, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1787743, Length=463, Percent_Identity=33.4773218142549, Blast_Score=189, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48885; Mature: 48885

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRHHVTQLNTKGVVLQGWDPEDPEHWDSKIAWRTLWITTFSMIIGFCVWYLVSAIAPLL
CCCCCCCEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRIGFDLSASQLYWLASIPGLAGGLIRLIYMFLPPILGTRKLVGISSALFLIPMFGWFLA
HHHCCCCCHHHEEEEHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEE
VQDSSTPYWWLLILAALTGIGGGVFSGYMPSTGYFFPKAKSGTALGIQAGIGNLGVSIIQ
EECCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHH
FMGPWVMGFGLLGIGFLTPQRTIEGSTVFVHNAAIVLVPWTILAAVLSFLFLKDVPVTAN
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEECHHHHHHHHHHHHHHCCCCCCC
FRQQIDIFGNKNTWILSIIYLMTFGAFAGFAAQFGLIINNNFGIASPMAETYPAEMLHAG
HHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCC
ATFAFLGPLIGALVRAAWGPLCDRFGGAIWTFVGGIGMTIATAAAAIFLSRAETPDDFWP
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
FLWSMLALFFFTGLGNAGTFKQMPMILPKRQAGGVIGWTGAIGAFGPFIVGVLLSFTPTV
HHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHCCCEEEECCCHHHHHHHHHHHHHHHHHHH
AFFWGCVVFFIIATALTWIYYARPNAPFPG
HHHHHHHHHHHHHHHHHHHHEECCCCCCCC
>Mature Secondary Structure
MKRHHVTQLNTKGVVLQGWDPEDPEHWDSKIAWRTLWITTFSMIIGFCVWYLVSAIAPLL
CCCCCCCEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRIGFDLSASQLYWLASIPGLAGGLIRLIYMFLPPILGTRKLVGISSALFLIPMFGWFLA
HHHCCCCCHHHEEEEHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEE
VQDSSTPYWWLLILAALTGIGGGVFSGYMPSTGYFFPKAKSGTALGIQAGIGNLGVSIIQ
EECCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHH
FMGPWVMGFGLLGIGFLTPQRTIEGSTVFVHNAAIVLVPWTILAAVLSFLFLKDVPVTAN
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEECHHHHHHHHHHHHHHCCCCCCC
FRQQIDIFGNKNTWILSIIYLMTFGAFAGFAAQFGLIINNNFGIASPMAETYPAEMLHAG
HHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCC
ATFAFLGPLIGALVRAAWGPLCDRFGGAIWTFVGGIGMTIATAAAAIFLSRAETPDDFWP
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
FLWSMLALFFFTGLGNAGTFKQMPMILPKRQAGGVIGWTGAIGAFGPFIVGVLLSFTPTV
HHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHCCCEEEECCCHHHHHHHHHHHHHHHHHHH
AFFWGCVVFFIIATALTWIYYARPNAPFPG
HHHHHHHHHHHHHHHHHHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]