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The map label for this gene is metE

Identifier: 145295282

GI number: 145295282

Start: 1330920

End: 1333157

Strand: Reverse

Name: metE

Synonym: cgR_1223

Alternate gene names: 145295282

Gene position: 1333157-1330920 (Counterclockwise)

Preceding gene: 145295287

Following gene: 145295279

Centisome position: 40.23

GC content: 57.02

Gene sequence:

>2238_bases
ATGACTTCCAACTTTTCTTCCACTGTCGCTGGTCTTCCTCGCATCGGAGCGAAGCGTGAACTGAAGTTCGCGCTCGAAGG
CTACTGGAATGGATCAATTGAAGGTCGCGAACTAGCGCAGACCGCCCGCCAATTGGTTAACACTGCATCGGATTCTTTGT
CTGGATTGGATTCCGTTCCGTTTGCAGGACGTTCCTACTACGACGCAATGCTCGATACCGCCGCTATTTTGGGTGTGCTG
CCGGAGCGTTTTGATGACATCGCTGATCATGAAAACGATGGTCTCCCACTGTGGATTGACCGCTACTTCGGCGCTGCTCG
CGGTACTGAGACCCTGCCTGCACAGGCAATGACCAAGTGGTTTGATACCAACTACCACTACCTCGTGCCGGAGTTGTCTG
CGGATACACGTTTCGTTTTGGATGCGTCTGCGCTGATTGAGGATCTCCGCTGCCAGCAGGTTCGTGGCGTTAATGCCCGC
CCTGTTCTGGTTGGTCCACTGACTTTTCTTTCCCTTGCTCGCACCACTGATGGTTCCAATCCTTTGGATCACCTGCCTGC
ACTGTTTGAGGTCTACGAGCGCCTCATCAAGTCTTTCGATACTGAGTGGGTTCAGATCGATGAGCCTGCGTTGGTCACCG
ATGTTGCTCCTGAGGTTTTGGAGCAGGTCCGCGCTGGTTACACCACTTTGGCTAAGCGCGGTGGCGTGTTTGTCAATACT
TACTTCGGCTCTGGCGATCAGGCGCTGAACACTCTTGCGGGTATTGGCCTTGGCGCGATTGGCGTTGACTTGGTCACCCA
TGGCGTCACTGAGCTTGCGGCGTGGAAGGGTGAGGAGCTGCTAGTTGCGGGCATCGTTGATGGTCGTAACATTTGGCGCA
CCGACCTGTGTGCTGCTCTTGCTTCCCTGAAGCGCCTGGCAGCTCGCGGCCCTATCGCAGTGTCCACTTCTTGCTCCCTG
CTGCACGTTCCTTACACCCTCGAGGCTGAGAACATTGAGCCTGAGGTCCGCGACTGGCTCGCCTTCGGCTCGGAGAAGAT
CACCGAGGTCAAGCTGCTTGCCGACGCCCTAGCCGGCAACATCGACGCGGCTGCGTTCGATGCGGCGTCCGCAGCAATTG
CTTCTCGACGCACCTCCCCACGCACCGCACCAATCACGCAGGAACTCCCTGGCCGTAGCCGTGGATCCTTCAACACTCGT
GTTACGCTGCAGGAGAAGTCACTGGAGCTTCCAGCTCTGCCAACCACCACCATTGGTTCTTTCCCACAGACCCCATCCAT
TCGTTCTGCTCGCGCTCGTCTGCGCAAGGAATCCATCACTTTGGAGCAGTACGAAGAGGCAATGCGCGAAGAAATCGATC
TGGTCATCGCCAAGCAGGAAGAACTTGGTCTTGATGTGTTGGTTCACGGTGAGCCAGAGCGCAACGACATGGTTCAGTAC
TTCTCTGAACTTCTCGACGGTTTCCTCTCAACCGCCAACGGCTGGGTCCAAAGCTACGGCTCCCGCTGTGTTCGTCCTCC
AGTGTTGTTCGGAAACGTTTCCCGACCAGCGCCAATGACTGTCAAGTGGTTCCAGTACGCACAGAGCCTGACCCAGAAAC
ATGTCAAGGGAATGCTCACCGGTCCAGTCACCATCCTTGCATGGTCCTTCGTTCGCGATGATCAGCCGCTGGCTACCACT
GCTGACCAGGTTGCACTGGCACTGCGCGATGAAATTAACGATCTCATCGAGGCTGGCGCGAAGATCATCCAGGTGGATGA
GCCTGCGATTCGTGAACTGTTGCCGCTACGAGACGTCGATAAGCCTGCCTACCTGCAGTGGTCCGTGGACTCCTTCCGCC
TGGCGACTGCCGGCGCACCCGACGACGTCCAAATCCACACCCACATGTGCTACTCCGAGTTCAACGAAGTGATCTCCTCG
GTCATCGCGTTGGATGCCGATGTCACCACCATCGAAGCAGCACGTTCCGACATGCAGGTCCTCGCTGCTCTGAAATCTTC
CGGCTTCGAGCTCGGCGTCGGACCTGGTGTGTGGGATATCCACTCCCCGCGCGTTCCTTCCGCGCAGGAAGTGGACGGTC
TCCTCGAGGCTGCACTGCAGTCCGTGGATCCTCGCCAGCTGTGGGTCAACCCAGACTGTGGTCTGAAGACCCGTGGATGG
CCAGAAGTGGAAGCTTCCCTAAAGGTTCTCGTTGAGTCCGCTAAGCAGGCTCGCGAGAAAATCGGAGCAACTATCTAA

Upstream 100 bases:

>100_bases
CACCCGGTGATTTCGCGAACCTTGAAACATCGTCAGAAGATTGCCGTGCGTCCTAGCCGGGATCCGCACGTTCGGCTCAA
GCAGAAAGTCTTTAACTCAC

Downstream 100 bases:

>100_bases
ATTGGGTTACCGCTAGGAACCCAAAGATTAAGGGCACGAGTGTCACCAGGATTGCCGCACCCATGGCAACACCGAAGGAC
ACCGTGCCCACTCCTATTTG

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 745; Mature: 744

Protein sequence:

>745_residues
MTSNFSSTVAGLPRIGAKRELKFALEGYWNGSIEGRELAQTARQLVNTASDSLSGLDSVPFAGRSYYDAMLDTAAILGVL
PERFDDIADHENDGLPLWIDRYFGAARGTETLPAQAMTKWFDTNYHYLVPELSADTRFVLDASALIEDLRCQQVRGVNAR
PVLVGPLTFLSLARTTDGSNPLDHLPALFEVYERLIKSFDTEWVQIDEPALVTDVAPEVLEQVRAGYTTLAKRGGVFVNT
YFGSGDQALNTLAGIGLGAIGVDLVTHGVTELAAWKGEELLVAGIVDGRNIWRTDLCAALASLKRLAARGPIAVSTSCSL
LHVPYTLEAENIEPEVRDWLAFGSEKITEVKLLADALAGNIDAAAFDAASAAIASRRTSPRTAPITQELPGRSRGSFNTR
VTLQEKSLELPALPTTTIGSFPQTPSIRSARARLRKESITLEQYEEAMREEIDLVIAKQEELGLDVLVHGEPERNDMVQY
FSELLDGFLSTANGWVQSYGSRCVRPPVLFGNVSRPAPMTVKWFQYAQSLTQKHVKGMLTGPVTILAWSFVRDDQPLATT
ADQVALALRDEINDLIEAGAKIIQVDEPAIRELLPLRDVDKPAYLQWSVDSFRLATAGAPDDVQIHTHMCYSEFNEVISS
VIALDADVTTIEAARSDMQVLAALKSSGFELGVGPGVWDIHSPRVPSAQEVDGLLEAALQSVDPRQLWVNPDCGLKTRGW
PEVEASLKVLVESAKQAREKIGATI

Sequences:

>Translated_745_residues
MTSNFSSTVAGLPRIGAKRELKFALEGYWNGSIEGRELAQTARQLVNTASDSLSGLDSVPFAGRSYYDAMLDTAAILGVL
PERFDDIADHENDGLPLWIDRYFGAARGTETLPAQAMTKWFDTNYHYLVPELSADTRFVLDASALIEDLRCQQVRGVNAR
PVLVGPLTFLSLARTTDGSNPLDHLPALFEVYERLIKSFDTEWVQIDEPALVTDVAPEVLEQVRAGYTTLAKRGGVFVNT
YFGSGDQALNTLAGIGLGAIGVDLVTHGVTELAAWKGEELLVAGIVDGRNIWRTDLCAALASLKRLAARGPIAVSTSCSL
LHVPYTLEAENIEPEVRDWLAFGSEKITEVKLLADALAGNIDAAAFDAASAAIASRRTSPRTAPITQELPGRSRGSFNTR
VTLQEKSLELPALPTTTIGSFPQTPSIRSARARLRKESITLEQYEEAMREEIDLVIAKQEELGLDVLVHGEPERNDMVQY
FSELLDGFLSTANGWVQSYGSRCVRPPVLFGNVSRPAPMTVKWFQYAQSLTQKHVKGMLTGPVTILAWSFVRDDQPLATT
ADQVALALRDEINDLIEAGAKIIQVDEPAIRELLPLRDVDKPAYLQWSVDSFRLATAGAPDDVQIHTHMCYSEFNEVISS
VIALDADVTTIEAARSDMQVLAALKSSGFELGVGPGVWDIHSPRVPSAQEVDGLLEAALQSVDPRQLWVNPDCGLKTRGW
PEVEASLKVLVESAKQAREKIGATI
>Mature_744_residues
TSNFSSTVAGLPRIGAKRELKFALEGYWNGSIEGRELAQTARQLVNTASDSLSGLDSVPFAGRSYYDAMLDTAAILGVLP
ERFDDIADHENDGLPLWIDRYFGAARGTETLPAQAMTKWFDTNYHYLVPELSADTRFVLDASALIEDLRCQQVRGVNARP
VLVGPLTFLSLARTTDGSNPLDHLPALFEVYERLIKSFDTEWVQIDEPALVTDVAPEVLEQVRAGYTTLAKRGGVFVNTY
FGSGDQALNTLAGIGLGAIGVDLVTHGVTELAAWKGEELLVAGIVDGRNIWRTDLCAALASLKRLAARGPIAVSTSCSLL
HVPYTLEAENIEPEVRDWLAFGSEKITEVKLLADALAGNIDAAAFDAASAAIASRRTSPRTAPITQELPGRSRGSFNTRV
TLQEKSLELPALPTTTIGSFPQTPSIRSARARLRKESITLEQYEEAMREEIDLVIAKQEELGLDVLVHGEPERNDMVQYF
SELLDGFLSTANGWVQSYGSRCVRPPVLFGNVSRPAPMTVKWFQYAQSLTQKHVKGMLTGPVTILAWSFVRDDQPLATTA
DQVALALRDEINDLIEAGAKIIQVDEPAIRELLPLRDVDKPAYLQWSVDSFRLATAGAPDDVQIHTHMCYSEFNEVISSV
IALDADVTTIEAARSDMQVLAALKSSGFELGVGPGVWDIHSPRVPSAQEVDGLLEAALQSVDPRQLWVNPDCGLKTRGWP
EVEASLKVLVESAKQAREKIGATI

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=752, Percent_Identity=45.3457446808511, Blast_Score=621, Evalue=1e-179,
Organism=Saccharomyces cerevisiae, GI6320936, Length=777, Percent_Identity=42.2136422136422, Blast_Score=562, Evalue=1e-161,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_CORGB (A4QDA4)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138103.1
- ProteinModelPortal:   A4QDA4
- SMR:   A4QDA4
- STRING:   A4QDA4
- GeneID:   4993780
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1223
- eggNOG:   COG0620
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 81255; Mature: 81124

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSNFSSTVAGLPRIGAKRELKFALEGYWNGSIEGRELAQTARQLVNTASDSLSGLDSVP
CCCCCCHHHHCCCCCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
FAGRSYYDAMLDTAAILGVLPERFDDIADHENDGLPLWIDRYFGAARGTETLPAQAMTKW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCCCCCCCCCCHHHHHHH
FDTNYHYLVPELSADTRFVLDASALIEDLRCQQVRGVNARPVLVGPLTFLSLARTTDGSN
HCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHCCCCCC
PLDHLPALFEVYERLIKSFDTEWVQIDEPALVTDVAPEVLEQVRAGYTTLAKRGGVFVNT
CHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
YFGSGDQALNTLAGIGLGAIGVDLVTHGVTELAAWKGEELLVAGIVDGRNIWRTDLCAAL
EECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
ASLKRLAARGPIAVSTSCSLLHVPYTLEAENIEPEVRDWLAFGSEKITEVKLLADALAGN
HHHHHHHHCCCEEEECCCCEEECCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC
IDAAAFDAASAAIASRRTSPRTAPITQELPGRSRGSFNTRVTLQEKSLELPALPTTTIGS
CCHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
FPQTPSIRSARARLRKESITLEQYEEAMREEIDLVIAKQEELGLDVLVHGEPERNDMVQY
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECHHHCCCEEEEECCCCHHHHHHH
FSELLDGFLSTANGWVQSYGSRCVRPPVLFGNVSRPAPMTVKWFQYAQSLTQKHVKGMLT
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GPVTILAWSFVRDDQPLATTADQVALALRDEINDLIEAGAKIIQVDEPAIRELLPLRDVD
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCCC
KPAYLQWSVDSFRLATAGAPDDVQIHTHMCYSEFNEVISSVIALDADVTTIEAARSDMQV
CCCEEEECCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LAALKSSGFELGVGPGVWDIHSPRVPSAQEVDGLLEAALQSVDPRQLWVNPDCGLKTRGW
HHHHHHCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
PEVEASLKVLVESAKQAREKIGATI
CHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSNFSSTVAGLPRIGAKRELKFALEGYWNGSIEGRELAQTARQLVNTASDSLSGLDSVP
CCCCCHHHHCCCCCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
FAGRSYYDAMLDTAAILGVLPERFDDIADHENDGLPLWIDRYFGAARGTETLPAQAMTKW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCCCCCCCCCCHHHHHHH
FDTNYHYLVPELSADTRFVLDASALIEDLRCQQVRGVNARPVLVGPLTFLSLARTTDGSN
HCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHCCCCCC
PLDHLPALFEVYERLIKSFDTEWVQIDEPALVTDVAPEVLEQVRAGYTTLAKRGGVFVNT
CHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
YFGSGDQALNTLAGIGLGAIGVDLVTHGVTELAAWKGEELLVAGIVDGRNIWRTDLCAAL
EECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
ASLKRLAARGPIAVSTSCSLLHVPYTLEAENIEPEVRDWLAFGSEKITEVKLLADALAGN
HHHHHHHHCCCEEEECCCCEEECCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC
IDAAAFDAASAAIASRRTSPRTAPITQELPGRSRGSFNTRVTLQEKSLELPALPTTTIGS
CCHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
FPQTPSIRSARARLRKESITLEQYEEAMREEIDLVIAKQEELGLDVLVHGEPERNDMVQY
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECHHHCCCEEEEECCCCHHHHHHH
FSELLDGFLSTANGWVQSYGSRCVRPPVLFGNVSRPAPMTVKWFQYAQSLTQKHVKGMLT
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GPVTILAWSFVRDDQPLATTADQVALALRDEINDLIEAGAKIIQVDEPAIRELLPLRDVD
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCCC
KPAYLQWSVDSFRLATAGAPDDVQIHTHMCYSEFNEVISSVIALDADVTTIEAARSDMQV
CCCEEEECCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LAALKSSGFELGVGPGVWDIHSPRVPSAQEVDGLLEAALQSVDPRQLWVNPDCGLKTRGW
HHHHHHCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
PEVEASLKVLVESAKQAREKIGATI
CHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA