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The map label for this gene is ydiB [C]

Identifier: 145295275

GI number: 145295275

Start: 1323374

End: 1324180

Strand: Reverse

Name: ydiB [C]

Synonym: cgR_1216

Alternate gene names: 145295275

Gene position: 1324180-1323374 (Counterclockwise)

Preceding gene: 145295279

Following gene: 145295274

Centisome position: 39.95

GC content: 52.79

Gene sequence:

>807_bases
ATGGTCAACTACGTCGACAGGGAAACAACCCTGTGCATCTCTCTCGCTGCTCGTCCATCCAACCATGGTGTTCGTTTCCA
CAACTGGCTTTACGCTGAACTTGGATTGAACTACCTGTACAAGGCTGTTGCCCCAGCAGATATCACCGCTGCAGTCGCAG
GTATCCGTGGTCTGAACATTCGCGGCGCAGGTGTCTCCATGCCATACAAGAGCGATGTCATCCCACTCATCGATGAGTTG
GATCCTTCCGCAGAGCGCATCCGTTCTGTTAACACCATCGTCAACAATGACGGACACCTTGTCGGATACAACACCGACTA
CACTGCTGTGTACCACCTCCTTGAAGAACACCGCGTGGACCCCAATGCACGAGTAGCTATCAAGGGATCCGGCGGCATGG
CCAATGCTGTTGTTGCAGCTCTTGCTGATTATGGTCTGAGTGGCACCGTCGTTGCCCGCAACCACACCACCGGTTCTGCG
CTAGCTTCCCGTTACGGTTGGGAATACTCCGCAACTGTTCCGGAAGACGCAAAAATTTTGGTTAATGTCACCCCAATGGG
AATGAATGGACCTGACCAAGACGTTGTATCTTTTGGTGAGGATGAAGTAGACCGAGCCGACGTAATCTTTGACTGCGTAG
CATTCCCCGTCGAGACCCCACTGATTAAGTTGGCCAAGGAAAAGGGTAAGCAAACCATCGACGGCGGAGAAGTTGCCGCT
CTTCAGGCAGCAGAGCAGTTCCACCTCTATACCGGAGTTCTTCCAACCAACGAGCAGATCATTGCTGCGGAGGAGTTCTC
CAAGTAA

Upstream 100 bases:

>100_bases
GGCAGGAATTTCCCGAAAACTTCCACCAATAATCAAGCCATATCCCACACAATCAGGCATTTGCTCTTCCTTTGCTCCGC
ATGAGTATAAAATCACTGTC

Downstream 100 bases:

>100_bases
ATTTCTCTCCCCTATCCTATTTTTAGGAGGCACCACATGGCTGAACACAACGCCATCATCACGGATGCAGTACATTCCGA
CCCCGCTGTTTTAGAAGACA

Product: shikimate 5-dehydrogenase

Products: 3-dehydroshikimate; NADPH; H+

Alternate protein names: SDH-L [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MVNYVDRETTLCISLAARPSNHGVRFHNWLYAELGLNYLYKAVAPADITAAVAGIRGLNIRGAGVSMPYKSDVIPLIDEL
DPSAERIRSVNTIVNNDGHLVGYNTDYTAVYHLLEEHRVDPNARVAIKGSGGMANAVVAALADYGLSGTVVARNHTTGSA
LASRYGWEYSATVPEDAKILVNVTPMGMNGPDQDVVSFGEDEVDRADVIFDCVAFPVETPLIKLAKEKGKQTIDGGEVAA
LQAAEQFHLYTGVLPTNEQIIAAEEFSK

Sequences:

>Translated_268_residues
MVNYVDRETTLCISLAARPSNHGVRFHNWLYAELGLNYLYKAVAPADITAAVAGIRGLNIRGAGVSMPYKSDVIPLIDEL
DPSAERIRSVNTIVNNDGHLVGYNTDYTAVYHLLEEHRVDPNARVAIKGSGGMANAVVAALADYGLSGTVVARNHTTGSA
LASRYGWEYSATVPEDAKILVNVTPMGMNGPDQDVVSFGEDEVDRADVIFDCVAFPVETPLIKLAKEKGKQTIDGGEVAA
LQAAEQFHLYTGVLPTNEQIIAAEEFSK
>Mature_268_residues
MVNYVDRETTLCISLAARPSNHGVRFHNWLYAELGLNYLYKAVAPADITAAVAGIRGLNIRGAGVSMPYKSDVIPLIDEL
DPSAERIRSVNTIVNNDGHLVGYNTDYTAVYHLLEEHRVDPNARVAIKGSGGMANAVVAALADYGLSGTVVARNHTTGSA
LASRYGWEYSATVPEDAKILVNVTPMGMNGPDQDVVSFGEDEVDRADVIFDCVAFPVETPLIKLAKEKGKQTIDGGEVAA
LQAAEQFHLYTGVLPTNEQIIAAEEFSK

Specific function: The physiological substrate is not known. Has much lower activity towards shikimate than AroE [H]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787983, Length=263, Percent_Identity=31.5589353612167, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1789675, Length=219, Percent_Identity=27.3972602739726, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR013708
- InterPro:   IPR006151 [H]

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N [H]

EC number: 1.1.1.25

Molecular weight: Translated: 28850; Mature: 28850

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNYVDRETTLCISLAARPSNHGVRFHNWLYAELGLNYLYKAVAPADITAAVAGIRGLNI
CCCCCCCCEEEEEEEEECCCCCCEEEHHEEEEHHHHHHHHHHCCCCHHHHHHHHCCCCEE
RGAGVSMPYKSDVIPLIDELDPSAERIRSVNTIVNNDGHLVGYNTDYTAVYHLLEEHRVD
ECCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHCCC
PNARVAIKGSGGMANAVVAALADYGLSGTVVARNHTTGSALASRYGWEYSATVPEDAKIL
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCEEECCCCCCCEEE
VNVTPMGMNGPDQDVVSFGEDEVDRADVIFDCVAFPVETPLIKLAKEKGKQTIDGGEVAA
EEEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEE
LQAAEQFHLYTGVLPTNEQIIAAEEFSK
HHHHHHHEEEECCCCCCCCEEECCCCCC
>Mature Secondary Structure
MVNYVDRETTLCISLAARPSNHGVRFHNWLYAELGLNYLYKAVAPADITAAVAGIRGLNI
CCCCCCCCEEEEEEEEECCCCCCEEEHHEEEEHHHHHHHHHHCCCCHHHHHHHHCCCCEE
RGAGVSMPYKSDVIPLIDELDPSAERIRSVNTIVNNDGHLVGYNTDYTAVYHLLEEHRVD
ECCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHCCC
PNARVAIKGSGGMANAVVAALADYGLSGTVVARNHTTGSALASRYGWEYSATVPEDAKIL
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCEEECCCCCCCEEE
VNVTPMGMNGPDQDVVSFGEDEVDRADVIFDCVAFPVETPLIKLAKEKGKQTIDGGEVAA
EEEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEE
LQAAEQFHLYTGVLPTNEQIIAAEEFSK
HHHHHHHEEEECCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: shikimate; NADP+

Specific reaction: shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]