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The map label for this gene is dapE [H]

Identifier: 145295251

GI number: 145295251

Start: 1298219

End: 1299328

Strand: Direct

Name: dapE [H]

Synonym: cgR_1192

Alternate gene names: 145295251

Gene position: 1298219-1299328 (Clockwise)

Preceding gene: 145295247

Following gene: 145295252

Centisome position: 39.17

GC content: 54.14

Gene sequence:

>1110_bases
GTGAACTCTGAACTCAAACCAGGATTAGATCTCCTCGGCGACCCAATTGTCCTTACTCAACGTTTGGTAGATATACCGAG
TCCGTCGGGTCAGGAAAAGCAGATTGCTGATGAAATTGAAGATGCCCTTCGGAACCTTAATCTACCTGGTGTAGAGGTCT
TCCGCTTCAACAACAACGTTCTTGCTCGCACGAACAGGGGATTGGCCTCGAGGGTCATGCTTGCTGGTCATATCGATACA
GTGCCGATCGCGGACAATCTGCCAAGCCGTGTGGAAGACGGCATCATGTATGGCTGTGGCACCGTCGATATGAAATCTGG
GTTGGCGGTGTATTTGCATACTTTTGCCACCTTGGCCACGTCGACTGAGCTTAAGCATGATCTGACGCTGATTGCGTATG
AGTGCGAGGAAGTTGCTGATCACCTCAATGGTTTGGGCCACATTCGCGATGAGCATCCGGAGTGGTTGGCGGCTGATTTG
GCGTTGTTGGGTGAGCCTACTGGCGGCTGGATTGAGGCGGGCTGCCAGGGCAATCTGCGCATCAAGGTGACGGCGCATGG
TGTGCGTGCCCATTCGGCGAGAAGCTGGTTGGGTGATAATGCGATGCATAAGTTGTCGCCGATCATTTCGAAGGTTGCTG
CGTATAAGGCCGCAGAAGTCAACATTGATGGCTTGACCTACCGTGAAGGCCTCAACATCGTTTTCTGCGAATCGGGCGTG
GCAAACAACGTCATTCCAGACCTCGCGTGGATGAACCTCAACTTCCGTTTCGCGCCGAATCGCGATATCAACGAGGCGAT
CGAGCATGTCGTCGAAACGCTTGAGCTTGACGGGCAAGACGGTATCGAATGGGCCGTAGAAGACGGGGCAGGCGGTGCCC
TTCCAGGCTTGGGGCAGCAGGTTACAAGCGGGCTTATCGACGCCGTCGGCCGCGAAAAAATCCGCGCAAAATTCGGCTGG
ACCGATGTCTCACGTTTTTCAGCCATGGGAATTCCAGCCCTAAACTTTGGCGCTGGTGATCCAAGCTTCGCGCACAAACG
CGACGAACAGTGCCCAGTGGAGCAAATCACGGATGTGGCAGCAATTTTGAAGCAGTACCTGAGCGAGTAA

Upstream 100 bases:

>100_bases
TATTTGCGATTCCAACTGCTTGGGCTCCGCGAATGTTTTCACTCATTTTTTAATCGACCGCTTCCATCATGTTTTAACTA
AGGTTTGTAGGCTTAAACCT

Downstream 100 bases:

>100_bases
CCGCATTCGGGGCTATCGTGGGACTTCCGAAATGAAACTAGAGACTTGAGGAGGAAACACGATGGCTCCTAAACAAACTC
CCAGCCCAGAGAAGAATCGA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MNSELKPGLDLLGDPIVLTQRLVDIPSPSGQEKQIADEIEDALRNLNLPGVEVFRFNNNVLARTNRGLASRVMLAGHIDT
VPIADNLPSRVEDGIMYGCGTVDMKSGLAVYLHTFATLATSTELKHDLTLIAYECEEVADHLNGLGHIRDEHPEWLAADL
ALLGEPTGGWIEAGCQGNLRIKVTAHGVRAHSARSWLGDNAMHKLSPIISKVAAYKAAEVNIDGLTYREGLNIVFCESGV
ANNVIPDLAWMNLNFRFAPNRDINEAIEHVVETLELDGQDGIEWAVEDGAGGALPGLGQQVTSGLIDAVGREKIRAKFGW
TDVSRFSAMGIPALNFGAGDPSFAHKRDEQCPVEQITDVAAILKQYLSE

Sequences:

>Translated_369_residues
MNSELKPGLDLLGDPIVLTQRLVDIPSPSGQEKQIADEIEDALRNLNLPGVEVFRFNNNVLARTNRGLASRVMLAGHIDT
VPIADNLPSRVEDGIMYGCGTVDMKSGLAVYLHTFATLATSTELKHDLTLIAYECEEVADHLNGLGHIRDEHPEWLAADL
ALLGEPTGGWIEAGCQGNLRIKVTAHGVRAHSARSWLGDNAMHKLSPIISKVAAYKAAEVNIDGLTYREGLNIVFCESGV
ANNVIPDLAWMNLNFRFAPNRDINEAIEHVVETLELDGQDGIEWAVEDGAGGALPGLGQQVTSGLIDAVGREKIRAKFGW
TDVSRFSAMGIPALNFGAGDPSFAHKRDEQCPVEQITDVAAILKQYLSE
>Mature_369_residues
MNSELKPGLDLLGDPIVLTQRLVDIPSPSGQEKQIADEIEDALRNLNLPGVEVFRFNNNVLARTNRGLASRVMLAGHIDT
VPIADNLPSRVEDGIMYGCGTVDMKSGLAVYLHTFATLATSTELKHDLTLIAYECEEVADHLNGLGHIRDEHPEWLAADL
ALLGEPTGGWIEAGCQGNLRIKVTAHGVRAHSARSWLGDNAMHKLSPIISKVAAYKAAEVNIDGLTYREGLNIVFCESGV
ANNVIPDLAWMNLNFRFAPNRDINEAIEHVVETLELDGQDGIEWAVEDGAGGALPGLGQQVTSGLIDAVGREKIRAKFGW
TDVSRFSAMGIPALNFGAGDPSFAHKRDEQCPVEQITDVAAILKQYLSE

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; fifth step. [C]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=258, Percent_Identity=27.5193798449612, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1790395, Length=377, Percent_Identity=24.1379310344828, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010174 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 39975; Mature: 39975

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSELKPGLDLLGDPIVLTQRLVDIPSPSGQEKQIADEIEDALRNLNLPGVEVFRFNNNV
CCCCCCCCHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCE
LARTNRGLASRVMLAGHIDTVPIADNLPSRVEDGIMYGCGTVDMKSGLAVYLHTFATLAT
EEECCCCHHHHHHEECCCCCCCCCCCCCHHHHCCEEECCCCCCHHCCHHHHHHHHHHHHH
STELKHDLTLIAYECEEVADHLNGLGHIRDEHPEWLAADLALLGEPTGGWIEAGCQGNLR
HHHHHCCEEEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCEE
IKVTAHGVRAHSARSWLGDNAMHKLSPIISKVAAYKAAEVNIDGLTYREGLNIVFCESGV
EEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCEEEECCCC
ANNVIPDLAWMNLNFRFAPNRDINEAIEHVVETLELDGQDGIEWAVEDGAGGALPGLGQQ
CCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHH
VTSGLIDAVGREKIRAKFGWTDVSRFSAMGIPALNFGAGDPSFAHKRDEQCPVEQITDVA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEECCCCCCCHHHCCCCCCCHHHHHHHH
AILKQYLSE
HHHHHHHCC
>Mature Secondary Structure
MNSELKPGLDLLGDPIVLTQRLVDIPSPSGQEKQIADEIEDALRNLNLPGVEVFRFNNNV
CCCCCCCCHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCE
LARTNRGLASRVMLAGHIDTVPIADNLPSRVEDGIMYGCGTVDMKSGLAVYLHTFATLAT
EEECCCCHHHHHHEECCCCCCCCCCCCCHHHHCCEEECCCCCCHHCCHHHHHHHHHHHHH
STELKHDLTLIAYECEEVADHLNGLGHIRDEHPEWLAADLALLGEPTGGWIEAGCQGNLR
HHHHHCCEEEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCEE
IKVTAHGVRAHSARSWLGDNAMHKLSPIISKVAAYKAAEVNIDGLTYREGLNIVFCESGV
EEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCEEEECCCC
ANNVIPDLAWMNLNFRFAPNRDINEAIEHVVETLELDGQDGIEWAVEDGAGGALPGLGQQ
CCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHH
VTSGLIDAVGREKIRAKFGWTDVSRFSAMGIPALNFGAGDPSFAHKRDEQCPVEQITDVA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEECCCCCCCHHHCCCCCCCHHHHHHHH
AILKQYLSE
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7881553; 12948626; 7592354 [H]