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The map label for this gene is chaA [H]

Identifier: 145295224

GI number: 145295224

Start: 1272122

End: 1273174

Strand: Direct

Name: chaA [H]

Synonym: cgR_1165

Alternate gene names: 145295224

Gene position: 1272122-1273174 (Clockwise)

Preceding gene: 145295223

Following gene: 145295225

Centisome position: 38.38

GC content: 51.09

Gene sequence:

>1053_bases
ATGCCGTTTTCTTGGCTAAAACCAATTGATTATGCCCGCATCTTTGTCGGCTGGGCATCGATTTTTATCATCCCCCTCAT
CACACTGCCATCAATTATTGAGTTGGCGCTGATCGTGGCAGTCATCCTATTCTGCGCATTTGGCGTGGTGAAGATGGCGG
AGCGTTTGGCTCATATTTTGGGTGATCCTTTTGGATCGTTGATCCTTACCTTGTCGATCGTGATCATTGAAGTGATTTTG
ATCTGTGCGGTGATGCTGGGGCCTGCTGATTCAACCACTGCTGGTCGGGATTCCGTGATGGCAGTGTCCATGATCATCAT
GGGTTTGGTCGTGGGATTGTGCCTACTCATTGGTGGTTTAAGGCATGGAAGCATGCCACACAATGGGGTGGGAACTCCGA
CCTACTTGGTGCTGATCGCAACTTTTTCCGTAATCGCTTTTGCGGTTCCAGCTTTCATGGGAAAATACTCCGCTGGGCAG
GCACTTGTTATTTCAACACTGACAGCAGTGGTGTACGGGTTCTTCCTGTTTCGCCAAATGGGTGCCCAAGCTGGTGAATT
TCAAGAGGTCGAGGTCGCAGAAAAGGCAGACGACGCAGCAAGATGGGAAGTCCCATTTAGAGGCTTAATCTTGATTATCA
CTGTGCTCCCCATCGTGTTGCTATCCCATGACATGGCCACCGTGATGGATGAAGTCCTGGCAAACCTCGGAGCACCAGTG
GCTATGGCCGGATTGATCATTGCCACCATCGTCTTCTTGCCAGAGACCATCACCTCCTTAAAAGCCGCGTGGACAGGAGA
GATTCAACGAGTAAGCAACCTCGCGCATGGAGCCCAAGTATCAACGGTGGGGCTGACAATCCCAGCTGTTCTAGTGATCG
GCGTGATCACAGGTCAAGATGTAGTTTTGGGGGAGACCCCGATCAACTTGTTGCTGCTGGGGACCACCATTGCGGTGACA
GCCATTGCGTTTAGCTCCAAGAAAGTCAGTGCTGTGCATGGCTCGGTGCTGCTCATGCTTTTCGGTGTTTACATGATGAG
CATGTTCGCCTGA

Upstream 100 bases:

>100_bases
GAGATTGTGCTAGGTTCTGATGAGGCTTCGGGACGACCCGAAGAAATCTATGACAGCCTGGGAACGGCCCAGAGTTCTTA
AGAAAGTTTGACTAGAGAAC

Downstream 100 bases:

>100_bases
TTTAGGTAGCCTGGTGGGAATGAGTGCACTTGAGACATTGCAATGGCAGGACTGGTCAAGCGTTTTAGTTGTGGTAGCTC
ACCCAGATGATCCGGAGTAT

Product: Ca2+/H+ antiporter

Products: Na (I) [Periplasm]; Ca (II) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MPFSWLKPIDYARIFVGWASIFIIPLITLPSIIELALIVAVILFCAFGVVKMAERLAHILGDPFGSLILTLSIVIIEVIL
ICAVMLGPADSTTAGRDSVMAVSMIIMGLVVGLCLLIGGLRHGSMPHNGVGTPTYLVLIATFSVIAFAVPAFMGKYSAGQ
ALVISTLTAVVYGFFLFRQMGAQAGEFQEVEVAEKADDAARWEVPFRGLILIITVLPIVLLSHDMATVMDEVLANLGAPV
AMAGLIIATIVFLPETITSLKAAWTGEIQRVSNLAHGAQVSTVGLTIPAVLVIGVITGQDVVLGETPINLLLLGTTIAVT
AIAFSSKKVSAVHGSVLLMLFGVYMMSMFA

Sequences:

>Translated_350_residues
MPFSWLKPIDYARIFVGWASIFIIPLITLPSIIELALIVAVILFCAFGVVKMAERLAHILGDPFGSLILTLSIVIIEVIL
ICAVMLGPADSTTAGRDSVMAVSMIIMGLVVGLCLLIGGLRHGSMPHNGVGTPTYLVLIATFSVIAFAVPAFMGKYSAGQ
ALVISTLTAVVYGFFLFRQMGAQAGEFQEVEVAEKADDAARWEVPFRGLILIITVLPIVLLSHDMATVMDEVLANLGAPV
AMAGLIIATIVFLPETITSLKAAWTGEIQRVSNLAHGAQVSTVGLTIPAVLVIGVITGQDVVLGETPINLLLLGTTIAVT
AIAFSSKKVSAVHGSVLLMLFGVYMMSMFA
>Mature_349_residues
PFSWLKPIDYARIFVGWASIFIIPLITLPSIIELALIVAVILFCAFGVVKMAERLAHILGDPFGSLILTLSIVIIEVILI
CAVMLGPADSTTAGRDSVMAVSMIIMGLVVGLCLLIGGLRHGSMPHNGVGTPTYLVLIATFSVIAFAVPAFMGKYSAGQA
LVISTLTAVVYGFFLFRQMGAQAGEFQEVEVAEKADDAARWEVPFRGLILIITVLPIVLLSHDMATVMDEVLANLGAPVA
MAGLIIATIVFLPETITSLKAAWTGEIQRVSNLAHGAQVSTVGLTIPAVLVIGVITGQDVVLGETPINLLLLGTTIAVTA
IAFSSKKVSAVHGSVLLMLFGVYMMSMFA

Specific function: According to PubMed:8496184 the natural activity of this protein is as a calcium/proton antiporter, but it can act as a weak sodium/proton antiporter. The presence of a preferred substrate and normal pH cytoplasmic values might prevent significant sodium/

COG id: COG0387

COG function: function code P; Ca2+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium/potassium/calcium exchanger family [H]

Homologues:

Organism=Escherichia coli, GI1787468, Length=336, Percent_Identity=31.547619047619, Blast_Score=164, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004713
- InterPro:   IPR004837 [H]

Pfam domain/function: PF01699 Na_Ca_ex [H]

EC number: NA

Molecular weight: Translated: 36953; Mature: 36822

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFSWLKPIDYARIFVGWASIFIIPLITLPSIIELALIVAVILFCAFGVVKMAERLAHIL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDPFGSLILTLSIVIIEVILICAVMLGPADSTTAGRDSVMAVSMIIMGLVVGLCLLIGGL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RHGSMPHNGVGTPTYLVLIATFSVIAFAVPAFMGKYSAGQALVISTLTAVVYGFFLFRQM
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
GAQAGEFQEVEVAEKADDAARWEVPFRGLILIITVLPIVLLSHDMATVMDEVLANLGAPV
CCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHH
AMAGLIIATIVFLPETITSLKAAWTGEIQRVSNLAHGAQVSTVGLTIPAVLVIGVITGQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
VVLGETPINLLLLGTTIAVTAIAFSSKKVSAVHGSVLLMLFGVYMMSMFA
EEECCCCCHHEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PFSWLKPIDYARIFVGWASIFIIPLITLPSIIELALIVAVILFCAFGVVKMAERLAHIL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDPFGSLILTLSIVIIEVILICAVMLGPADSTTAGRDSVMAVSMIIMGLVVGLCLLIGGL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RHGSMPHNGVGTPTYLVLIATFSVIAFAVPAFMGKYSAGQALVISTLTAVVYGFFLFRQM
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
GAQAGEFQEVEVAEKADDAARWEVPFRGLILIITVLPIVLLSHDMATVMDEVLANLGAPV
CCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHH
AMAGLIIATIVFLPETITSLKAAWTGEIQRVSNLAHGAQVSTVGLTIPAVLVIGVITGQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
VVLGETPINLLLLGTTIAVTAIAFSSKKVSAVHGSVLLMLFGVYMMSMFA
EEECCCCCHHEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm]; Ca (II) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + Na (I) [Periplasm] Ca (II) [Cytoplasm] + Proton [Periplasm] = Ca (II) [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8496184; 8905232; 9278503 [H]