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The map label for this gene is glpX

Identifier: 145295161

GI number: 145295161

Start: 1214547

End: 1215554

Strand: Reverse

Name: glpX

Synonym: cgR_1103

Alternate gene names: 145295161

Gene position: 1215554-1214547 (Counterclockwise)

Preceding gene: 145295163

Following gene: 145295160

Centisome position: 36.68

GC content: 56.65

Gene sequence:

>1008_bases
ATGAACCTTAAGAACCCCGAAACGCCAGACCGTAACCTTGCTATGGAGCTGGTGCGAGTTACGGAAGCAGCTGCACTGGC
TTCTGGACGTTGGGTTGGACGTGGCATGAAGAATGAAGGCGACGGTGCTGCTGTTGACGCCATGCGCCAGCTCATCAACT
CAGTGACCATGAAGGGTGTCGTTGTTATCGGCGAGGGCGAAAAAGACGAAGCTCCAATGCTGTACAACGGCGAAGAAGTC
GGAACCGGCTTTGGACCTGAGGTAGATATCGCAGTTGACCCAGTTGACGGCACCACCCTGATGGCTGAGGGTCGCCCCAA
CGCAATTTCCATCCTTGCAGCTGCAGAGCGTGGCACCATGTACGATCCATCCTCCGTCTTCTACATGAAGAAGATTGCTG
TGGGACCTGAGGCCGCAGGCAAGATTGACATCGAAGCTCCAGTTGCCCACAACATCAACGCAGTGGCAAAGTCCAAGGGA
ATCAACCCTTCCGACGTCACCGTTGTCGTGCTTGACCGTCCTCGCCACATCGAACTCATCGCGGACATTCGTCGTGCAGG
CGCAAAAGTTCGGCTCATCTCCGACGGCGACGTTGCAGGTGCAGTTGCAGCAGCTCAGGACTCCAACTCCGTGGACATCA
TGATGGGTACCGGCGGAACCCCAGAAGGCATCATCACAGCTTGCGCCATGAAGTGCATGGGTGGCGAAATCCAGGGCATC
CTGGCCCCAATGAACGATTTCGAGCGCCAGAAGGCACACGACGCTGGACTGGTTCTTGATCAGGTTCTGCACACCAACGA
TCTGGTGAGCTCCGACAATTGCTACTTCGTGGCAACCGGTGTGACCAACGGTGACATGCTCCGCGGTGTTTCCTACCGTG
CAAACGGCGCAACCACCCGTTCCCTAGTTATGCGCGCAAAGTCCGGCACTATCCGCCACATCGAGTCCGTCCACCAGCTG
TCCAAGCTGCAGGAATACTCCGTGGTTGACTACACCACCGCAACCTAA

Upstream 100 bases:

>100_bases
GCGGGTTGATACAGCCCAAGCGCCGATACATTTATAATGCGCCTCGATACGTGCACCCCACGTAACCAGGTCAGATCAAG
TGCCCCAGGAGGCCCTGCAG

Downstream 100 bases:

>100_bases
GAGCTCTTAGATCAAAAACCGCCGTCCATTGTGGTCGGCGGTTTTTGCTTGTCGACGCCCCCACCTGGTGCTTTCTGAAA
ACTGAAGAATTTACTTGCGC

Product: fructose 1,6-bisphosphatase II

Products: NA

Alternate protein names: FBPase class 2; D-fructose-1,6-bisphosphate 1-phosphohydrolase class 2

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MNLKNPETPDRNLAMELVRVTEAAALASGRWVGRGMKNEGDGAAVDAMRQLINSVTMKGVVVIGEGEKDEAPMLYNGEEV
GTGFGPEVDIAVDPVDGTTLMAEGRPNAISILAAAERGTMYDPSSVFYMKKIAVGPEAAGKIDIEAPVAHNINAVAKSKG
INPSDVTVVVLDRPRHIELIADIRRAGAKVRLISDGDVAGAVAAAQDSNSVDIMMGTGGTPEGIITACAMKCMGGEIQGI
LAPMNDFERQKAHDAGLVLDQVLHTNDLVSSDNCYFVATGVTNGDMLRGVSYRANGATTRSLVMRAKSGTIRHIESVHQL
SKLQEYSVVDYTTAT

Sequences:

>Translated_335_residues
MNLKNPETPDRNLAMELVRVTEAAALASGRWVGRGMKNEGDGAAVDAMRQLINSVTMKGVVVIGEGEKDEAPMLYNGEEV
GTGFGPEVDIAVDPVDGTTLMAEGRPNAISILAAAERGTMYDPSSVFYMKKIAVGPEAAGKIDIEAPVAHNINAVAKSKG
INPSDVTVVVLDRPRHIELIADIRRAGAKVRLISDGDVAGAVAAAQDSNSVDIMMGTGGTPEGIITACAMKCMGGEIQGI
LAPMNDFERQKAHDAGLVLDQVLHTNDLVSSDNCYFVATGVTNGDMLRGVSYRANGATTRSLVMRAKSGTIRHIESVHQL
SKLQEYSVVDYTTAT
>Mature_335_residues
MNLKNPETPDRNLAMELVRVTEAAALASGRWVGRGMKNEGDGAAVDAMRQLINSVTMKGVVVIGEGEKDEAPMLYNGEEV
GTGFGPEVDIAVDPVDGTTLMAEGRPNAISILAAAERGTMYDPSSVFYMKKIAVGPEAAGKIDIEAPVAHNINAVAKSKG
INPSDVTVVVLDRPRHIELIADIRRAGAKVRLISDGDVAGAVAAAQDSNSVDIMMGTGGTPEGIITACAMKCMGGEIQGI
LAPMNDFERQKAHDAGLVLDQVLHTNDLVSSDNCYFVATGVTNGDMLRGVSYRANGATTRSLVMRAKSGTIRHIESVHQL
SKLQEYSVVDYTTAT

Specific function: Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate. Is essential for growth on gluconeogenic carbon sources. Also displays a low activity toward glucose 6- phosphate, and fructose 6-phosphate, glycerol 3-phosphate, ribulose 1,5-

COG id: COG1494

COG function: function code G; Fructose-1,6-bisphosphatase/sedoheptulose 1,7-bisphosphatase and related proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FBPase class 2 family

Homologues:

Organism=Escherichia coli, GI1790360, Length=325, Percent_Identity=44.3076923076923, Blast_Score=274, Evalue=7e-75,
Organism=Escherichia coli, GI1789298, Length=323, Percent_Identity=42.1052631578947, Blast_Score=234, Evalue=5e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLPX_CORGL (Q6M6E7)

Other databases:

- EMBL:   BA000036
- EMBL:   BX927151
- RefSeq:   NP_600242.1
- RefSeq:   YP_225307.1
- ProteinModelPortal:   Q6M6E7
- SMR:   Q6M6E7
- World-2DPAGE:   0001:Q6M6E7
- PRIDE:   Q6M6E7
- GeneID:   1019005
- GeneID:   3343264
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg1157
- OMA:   CVLDRPR
- PhylomeDB:   Q6M6E7
- ProtClustDB:   PRK09479
- BioCyc:   CGLU196627:CG1157-MONOMER
- GO:   GO:0006094
- InterPro:   IPR004464
- PIRSF:   PIRSF004532
- TIGRFAMs:   TIGR00330

Pfam domain/function: PF03320 FBPase_glpX

EC number: =3.1.3.11

Molecular weight: Translated: 35370; Mature: 35370

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: BINDING 128-128 BINDING 219-219

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKNPETPDRNLAMELVRVTEAAALASGRWVGRGMKNEGDGAAVDAMRQLINSVTMKGV
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCEE
VVIGEGEKDEAPMLYNGEEVGTGFGPEVDIAVDPVDGTTLMAEGRPNAISILAAAERGTM
EEEECCCCCCCCEEECCCCCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEEEECCCCC
YDPSSVFYMKKIAVGPEAAGKIDIEAPVAHNINAVAKSKGINPSDVTVVVLDRPRHIELI
CCCCCEEEEEEEECCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCHHH
ADIRRAGAKVRLISDGDVAGAVAAAQDSNSVDIMMGTGGTPEGIITACAMKCMGGEIQGI
HHHHHCCCEEEEEECCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEE
LAPMNDFERQKAHDAGLVLDQVLHTNDLVSSDNCYFVATGVTNGDMLRGVSYRANGATTR
ECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHCCEEEECCCCCHH
SLVMRAKSGTIRHIESVHQLSKLQEYSVVDYTTAT
HHEEECCCCCHHHHHHHHHHHHHHHCEEEEEEECC
>Mature Secondary Structure
MNLKNPETPDRNLAMELVRVTEAAALASGRWVGRGMKNEGDGAAVDAMRQLINSVTMKGV
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCEE
VVIGEGEKDEAPMLYNGEEVGTGFGPEVDIAVDPVDGTTLMAEGRPNAISILAAAERGTM
EEEECCCCCCCCEEECCCCCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEEEECCCCC
YDPSSVFYMKKIAVGPEAAGKIDIEAPVAHNINAVAKSKGINPSDVTVVVLDRPRHIELI
CCCCCEEEEEEEECCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCHHH
ADIRRAGAKVRLISDGDVAGAVAAAQDSNSVDIMMGTGGTPEGIITACAMKCMGGEIQGI
HHHHHCCCEEEEEECCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEE
LAPMNDFERQKAHDAGLVLDQVLHTNDLVSSDNCYFVATGVTNGDMLRGVSYRANGATTR
ECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHCCEEEECCCCCHH
SLVMRAKSGTIRHIESVHQLSKLQEYSVVDYTTAT
HHEEECCCCCHHHHHHHHHHHHHHHCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626