Click here to switch to the map view.

The map label for this gene is eno [H]

Identifier: 145295128

GI number: 145295128

Start: 1184923

End: 1186200

Strand: Direct

Name: eno [H]

Synonym: cgR_1071

Alternate gene names: 145295128

Gene position: 1184923-1186200 (Clockwise)

Preceding gene: 145295127

Following gene: 145295129

Centisome position: 35.75

GC content: 57.82

Gene sequence:

>1278_bases
GTGGCTGAAATCATGCACGTATTCGCTCGCGAAATTCTCGACTCCCGCGGTAACCCAACCGTCGAGGCAGAGGTTTTCCT
GGATGACGGTTCCCACGGTGTCGCAGGTGTTCCATCCGGCGCATCCACCGGCGTCCACGAGGCTCACGAGCTGCGTGACG
GTGGCGATCGCTACCTGGGCAAGGGCGTTTTGAAGGCAGTTGAAAACGTCAACGAAGAAATCGGCGACGAGCTCGCTGGC
CTAGAGGCTGACGATCAGCGCCTCATCGACGAAGCAATGATCAAGCTTGATGGCACCGCCAACAAGTCCCGCCTGGGTGC
AAACGCAATCCTTGGTGTTTCCATGGCTGTTGCAAAGGCTGCTGCTGATTCCGCAGGCCTCCCACTGTTCCGCTACATCG
GTGGACCAAACGCACACGTTCTTCCAGTTCCAATGATGAACATCATCAACGGTGGCGCTCACGCTGACTCCGGTGTTGAC
GTTCAGGAATTCATGATCGCTCCAATCGGTGCAGAGACCTTCTCTGAGGCTCTCCGCAACGGCGCAGAGGTCTACCACGC
ACTGAAGTCTGTCATCAAGGAAAAGGGCCTGTCCACCGGACTTGGCGATGAGGGCGGCTTCGCTCCTTCCGTCGGCTCCA
CCCGTGAGGCTCTTGACCTTATCGTTGAGGCAATCGAGAAGGCTGGCTTCACCCCAGGCAAGGACATCGCTCTTGCTCTG
GACGTTGCTTCCTCTGAGTTCTTCAAGGACGGCACCTACCACTTCGAAGGTGGCCAGCACTCCGCAGCTGAGATGGCAAA
CGTTTACGCTGAGCTCGTTGACGCGTACCCAATCGTCTCCATCGAGGACCCACTGCAGGAAGATGACTGGGAGGGTTACA
CCAACCTCACCGCAACCATCGGTGACAAGGTTCAGATCGTTGGCGACGACTTCTTCGTCACCAACCCTGAGCGCCTGAAG
GAGGGCATTGCTAAGAAGGCTGCCAACTCCATCCTGGTTAAGGTGAACCAGATCGGTACCCTGACTGAGACCTTCGACGC
TGTCGACATGGCTCACCGCGCAGGCTACACCTCCATGATGTCTCACCGTTCCGGTGAGACCGAGGACACCACTATTGCTG
ACCTCGCAGTTGCACTTAACTGTGGCCAGATCAAGACTGGTGCTCCAGCACGTTCCGACCGTGTCGCAAAGTACAACCAG
CTTCTCCGCATCGAGCAGCTGCTTGGCGACGCCGGCGTCTACGCAGGTCGCAGCGCATTCCCACGCTTTCAGGGCTAA

Upstream 100 bases:

>100_bases
CGAAACAAGATTCGTGCAACAATTGGGTGTAGACGTGATTGAACACATTTGATCACGTGAATAATTCTAGTTAGCTCCCA
AGTTGGCATAGGAGGCCACA

Downstream 100 bases:

>100_bases
ATAAAAGCGCTTTTCGACGCCCGGTTACCTCTTGGTTGCCGGGCGTCGTTGCCTTACTACTGTTACTGGTGTGACTATGA
TCGAGGATTATGGCAAAGCA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MAEIMHVFAREILDSRGNPTVEAEVFLDDGSHGVAGVPSGASTGVHEAHELRDGGDRYLGKGVLKAVENVNEEIGDELAG
LEADDQRLIDEAMIKLDGTANKSRLGANAILGVSMAVAKAAADSAGLPLFRYIGGPNAHVLPVPMMNIINGGAHADSGVD
VQEFMIAPIGAETFSEALRNGAEVYHALKSVIKEKGLSTGLGDEGGFAPSVGSTREALDLIVEAIEKAGFTPGKDIALAL
DVASSEFFKDGTYHFEGGQHSAAEMANVYAELVDAYPIVSIEDPLQEDDWEGYTNLTATIGDKVQIVGDDFFVTNPERLK
EGIAKKAANSILVKVNQIGTLTETFDAVDMAHRAGYTSMMSHRSGETEDTTIADLAVALNCGQIKTGAPARSDRVAKYNQ
LLRIEQLLGDAGVYAGRSAFPRFQG

Sequences:

>Translated_425_residues
MAEIMHVFAREILDSRGNPTVEAEVFLDDGSHGVAGVPSGASTGVHEAHELRDGGDRYLGKGVLKAVENVNEEIGDELAG
LEADDQRLIDEAMIKLDGTANKSRLGANAILGVSMAVAKAAADSAGLPLFRYIGGPNAHVLPVPMMNIINGGAHADSGVD
VQEFMIAPIGAETFSEALRNGAEVYHALKSVIKEKGLSTGLGDEGGFAPSVGSTREALDLIVEAIEKAGFTPGKDIALAL
DVASSEFFKDGTYHFEGGQHSAAEMANVYAELVDAYPIVSIEDPLQEDDWEGYTNLTATIGDKVQIVGDDFFVTNPERLK
EGIAKKAANSILVKVNQIGTLTETFDAVDMAHRAGYTSMMSHRSGETEDTTIADLAVALNCGQIKTGAPARSDRVAKYNQ
LLRIEQLLGDAGVYAGRSAFPRFQG
>Mature_424_residues
AEIMHVFAREILDSRGNPTVEAEVFLDDGSHGVAGVPSGASTGVHEAHELRDGGDRYLGKGVLKAVENVNEEIGDELAGL
EADDQRLIDEAMIKLDGTANKSRLGANAILGVSMAVAKAAADSAGLPLFRYIGGPNAHVLPVPMMNIINGGAHADSGVDV
QEFMIAPIGAETFSEALRNGAEVYHALKSVIKEKGLSTGLGDEGGFAPSVGSTREALDLIVEAIEKAGFTPGKDIALALD
VASSEFFKDGTYHFEGGQHSAAEMANVYAELVDAYPIVSIEDPLQEDDWEGYTNLTATIGDKVQIVGDDFFVTNPERLKE
GIAKKAANSILVKVNQIGTLTETFDAVDMAHRAGYTSMMSHRSGETEDTTIADLAVALNCGQIKTGAPARSDRVAKYNQL
LRIEQLLGDAGVYAGRSAFPRFQG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=53.3642691415313, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=53.3642691415313, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=52.7906976744186, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=51.8604651162791, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI301897479, Length=429, Percent_Identity=48.018648018648, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI169201331, Length=339, Percent_Identity=24.188790560472, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI169201757, Length=339, Percent_Identity=24.188790560472, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI239744207, Length=339, Percent_Identity=24.188790560472, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=59.2941176470588, Blast_Score=476, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=53.1322505800464, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=53.1322505800464, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32563855, Length=215, Percent_Identity=46.9767441860465, Blast_Score=191, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=53.5796766743649, Blast_Score=420, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6324974, Length=431, Percent_Identity=51.7401392111369, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324969, Length=431, Percent_Identity=51.7401392111369, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6323985, Length=431, Percent_Identity=51.508120649652, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=53.3487297921478, Blast_Score=399, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=54.5243619489559, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=54.5243619489559, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=54.5243619489559, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=54.5243619489559, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=54.5243619489559, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=54.5243619489559, Blast_Score=414, Evalue=1e-116,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 44950; Mature: 44819

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIMHVFAREILDSRGNPTVEAEVFLDDGSHGVAGVPSGASTGVHEAHELRDGGDRYLG
CHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHH
KGVLKAVENVNEEIGDELAGLEADDQRLIDEAMIKLDGTANKSRLGANAILGVSMAVAKA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHH
AADSAGLPLFRYIGGPNAHVLPVPMMNIINGGAHADSGVDVQEFMIAPIGAETFSEALRN
HCCCCCCCHHHHCCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHH
GAEVYHALKSVIKEKGLSTGLGDEGGFAPSVGSTREALDLIVEAIEKAGFTPGKDIALAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
DVASSEFFKDGTYHFEGGQHSAAEMANVYAELVDAYPIVSIEDPLQEDDWEGYTNLTATI
EEHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCEEEEC
GDKVQIVGDDFFVTNPERLKEGIAKKAANSILVKVNQIGTLTETFDAVDMAHRAGYTSMM
CCEEEEEECCEEECCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHH
SHRSGETEDTTIADLAVALNCGQIKTGAPARSDRVAKYNQLLRIEQLLGDAGVYAGRSAF
HCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PRFQG
CCCCC
>Mature Secondary Structure 
AEIMHVFAREILDSRGNPTVEAEVFLDDGSHGVAGVPSGASTGVHEAHELRDGGDRYLG
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHH
KGVLKAVENVNEEIGDELAGLEADDQRLIDEAMIKLDGTANKSRLGANAILGVSMAVAKA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHH
AADSAGLPLFRYIGGPNAHVLPVPMMNIINGGAHADSGVDVQEFMIAPIGAETFSEALRN
HCCCCCCCHHHHCCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHH
GAEVYHALKSVIKEKGLSTGLGDEGGFAPSVGSTREALDLIVEAIEKAGFTPGKDIALAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
DVASSEFFKDGTYHFEGGQHSAAEMANVYAELVDAYPIVSIEDPLQEDDWEGYTNLTATI
EEHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCEEEEC
GDKVQIVGDDFFVTNPERLKEGIAKKAANSILVKVNQIGTLTETFDAVDMAHRAGYTSMM
CCEEEEEECCEEECCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHH
SHRSGETEDTTIADLAVALNCGQIKTGAPARSDRVAKYNQLLRIEQLLGDAGVYAGRSAF
HCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PRFQG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]