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The map label for this gene is amiC [H]

Identifier: 145295088

GI number: 145295088

Start: 1139942

End: 1141213

Strand: Direct

Name: amiC [H]

Synonym: cgR_1031

Alternate gene names: 145295088

Gene position: 1139942-1141213 (Clockwise)

Preceding gene: 145295086

Following gene: 145295089

Centisome position: 34.4

GC content: 55.58

Gene sequence:

>1272_bases
ATGTCCCGTCCAATCGTTAAACAAGCATTCACCGTCACCGCAGTCACCGCGATGGCTTTGGCCCTGGCATCATGCACCCG
CGCAGTGGATGCAACCTCCGCAGATGGAACAGTGAGCAACACCGCCGCTTCCTGTGTGGATACATCCGGCGACTCCATCA
AAATCGGCTTCATCAACTCCCTGTCTGGCACCATGGCAATCTCTGAAACCACGGTGAACCAATCCCTGCACATGGCAGCC
GATGAAATCAACGCAGCCGGCGGCGTTTTGGGCAAGCAGTTGGAGATCTCTGAAGAAGACGGCGCCAGCGAACCCGCCAC
CTTCGCCGAGCGCTCCCAACGCCTCATCCAGCAGGAATGCGTTGCAGCAGTGTTTGGTGGATGGACCTCCGCCTCCCGCA
AAGCAATGCTCCCTGTTTTTGAGGGCAATAACTCCCTGCTGTTCTACCCGGTGCAGTACGAGGGCATGGAATCCTCGCCG
AATATTTTCTACACCGGCGCCACCACCAACCAGCAGATCATCCCGGCTCTTGATTACCTGCGTGAAAACGGCCTGAACCG
CCTTTTCCTTGTCGGTTCCGATTATGTTTTCCCACGCACTGCAAATTCCATCATCAAGGACTACGCCGAAGCCAATGGTA
TGGAAATCGTCGGCGAAGACTACGCGCCGTTGGGATCCACCGACTTCACCACCATCGCCAACCGCATGCGTGACTCCAAC
GCAGATGCTGTGTTCAACACTTTGAATGGCGATTCCAACGTGGCGTTCTTCCGCCAGTACAACAGCCTCGGCTTCAATGC
AGACACCCTTCCGGTGATGTCAGTATCCATTGCGGAAGAAGAAGTCGGAGGCATCGGCACCGCAAATATTGAGGGCCAGC
TGGTGGCGTGGGACTACTACCAAACCATCGACACCCCAGAAAACGAGACCTTCGTGGAGAATTTCAAGGACCTCTACGGC
CAGGACAAAGTGACCTCCGACCCGATGGAAGCTGCTTACACTAGCCTCTACCTCTGGAAAGAAATGGTAGAGAAGGCCGA
TTCCTTTGATGTCGCCGCAATTCAAGCAGCCGCCGACGGAACCACTTTTGATGCACCAGAAGGAACCGTGGTGGTTGACG
GCGATAACCACCACATCTCCAAAACACCGCGCATCGGTCGAATCCGCCCGGATGGATTGATCGACACCATTTGGGAAACC
GATTCCCCAGTTGATCCGGACCCATACCTGTCTTCCTATGACTGGGCCAAGACCACCGCTGCGACTTCCTAA

Upstream 100 bases:

>100_bases
AATACACGTCACTGCATTTAAGCTCAAAAACAATTACCTATAGGCTGACAGAAACTCTAAAAACTATAGAGCTATAGAAA
CCTTAACTTCGGAGGTATTC

Downstream 100 bases:

>100_bases
GAGATAAAAATCATGGACATTTTACTCAATCAGCTCGTAGCCGGGCTTTCAGTTGGATCGGTCCTTCTATTGGTCGCAGT
GGGATTGTCACTGACCTTTG

Product: hypothetical protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSRPIVKQAFTVTAVTAMALALASCTRAVDATSADGTVSNTAASCVDTSGDSIKIGFINSLSGTMAISETTVNQSLHMAA
DEINAAGGVLGKQLEISEEDGASEPATFAERSQRLIQQECVAAVFGGWTSASRKAMLPVFEGNNSLLFYPVQYEGMESSP
NIFYTGATTNQQIIPALDYLRENGLNRLFLVGSDYVFPRTANSIIKDYAEANGMEIVGEDYAPLGSTDFTTIANRMRDSN
ADAVFNTLNGDSNVAFFRQYNSLGFNADTLPVMSVSIAEEEVGGIGTANIEGQLVAWDYYQTIDTPENETFVENFKDLYG
QDKVTSDPMEAAYTSLYLWKEMVEKADSFDVAAIQAAADGTTFDAPEGTVVVDGDNHHISKTPRIGRIRPDGLIDTIWET
DSPVDPDPYLSSYDWAKTTAATS

Sequences:

>Translated_423_residues
MSRPIVKQAFTVTAVTAMALALASCTRAVDATSADGTVSNTAASCVDTSGDSIKIGFINSLSGTMAISETTVNQSLHMAA
DEINAAGGVLGKQLEISEEDGASEPATFAERSQRLIQQECVAAVFGGWTSASRKAMLPVFEGNNSLLFYPVQYEGMESSP
NIFYTGATTNQQIIPALDYLRENGLNRLFLVGSDYVFPRTANSIIKDYAEANGMEIVGEDYAPLGSTDFTTIANRMRDSN
ADAVFNTLNGDSNVAFFRQYNSLGFNADTLPVMSVSIAEEEVGGIGTANIEGQLVAWDYYQTIDTPENETFVENFKDLYG
QDKVTSDPMEAAYTSLYLWKEMVEKADSFDVAAIQAAADGTTFDAPEGTVVVDGDNHHISKTPRIGRIRPDGLIDTIWET
DSPVDPDPYLSSYDWAKTTAATS
>Mature_422_residues
SRPIVKQAFTVTAVTAMALALASCTRAVDATSADGTVSNTAASCVDTSGDSIKIGFINSLSGTMAISETTVNQSLHMAAD
EINAAGGVLGKQLEISEEDGASEPATFAERSQRLIQQECVAAVFGGWTSASRKAMLPVFEGNNSLLFYPVQYEGMESSPN
IFYTGATTNQQIIPALDYLRENGLNRLFLVGSDYVFPRTANSIIKDYAEANGMEIVGEDYAPLGSTDFTTIANRMRDSNA
DAVFNTLNGDSNVAFFRQYNSLGFNADTLPVMSVSIAEEEVGGIGTANIEGQLVAWDYYQTIDTPENETFVENFKDLYGQ
DKVTSDPMEAAYTSLYLWKEMVEKADSFDVAAIQAAADGTTFDAPEGTVVVDGDNHHISKTPRIGRIRPDGLIDTIWETD
SPVDPDPYLSSYDWAKTTAATS

Specific function: Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 45561; Mature: 45429

Theoretical pI: Translated: 3.96; Mature: 3.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPIVKQAFTVTAVTAMALALASCTRAVDATSADGTVSNTAASCVDTSGDSIKIGFINS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCEEEEEEEEC
LSGTMAISETTVNQSLHMAADEINAAGGVLGKQLEISEEDGASEPATFAERSQRLIQQEC
CCCCEEEEHHHHCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHH
VAAVFGGWTSASRKAMLPVFEGNNSLLFYPVQYEGMESSPNIFYTGATTNQQIIPALDYL
HHHHHCCCCCCCCCEEEEEEECCCEEEEEEEEECCCCCCCCEEEECCCCCCCHHHHHHHH
RENGLNRLFLVGSDYVFPRTANSIIKDYAEANGMEIVGEDYAPLGSTDFTTIANRMRDSN
HHCCCCEEEEEECCEECCCCHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHCCCC
ADAVFNTLNGDSNVAFFRQYNSLGFNADTLPVMSVSIAEEEVGGIGTANIEGQLVAWDYY
CCCEEEECCCCCCEEEEEECCCCCCCCCCCEEEEEEECHHHHCCCEECCCCCEEEEEEHH
QTIDTPENETFVENFKDLYGQDKVTSDPMEAAYTSLYLWKEMVEKADSFDVAAIQAAADG
ECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
TTFDAPEGTVVVDGDNHHISKTPRIGRIRPDGLIDTIWETDSPVDPDPYLSSYDWAKTTA
CCCCCCCCEEEEECCCCEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCC
ATS
CCC
>Mature Secondary Structure 
SRPIVKQAFTVTAVTAMALALASCTRAVDATSADGTVSNTAASCVDTSGDSIKIGFINS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCEEEEEEEEC
LSGTMAISETTVNQSLHMAADEINAAGGVLGKQLEISEEDGASEPATFAERSQRLIQQEC
CCCCEEEEHHHHCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHH
VAAVFGGWTSASRKAMLPVFEGNNSLLFYPVQYEGMESSPNIFYTGATTNQQIIPALDYL
HHHHHCCCCCCCCCEEEEEEECCCEEEEEEEEECCCCCCCCEEEECCCCCCCHHHHHHHH
RENGLNRLFLVGSDYVFPRTANSIIKDYAEANGMEIVGEDYAPLGSTDFTTIANRMRDSN
HHCCCCEEEEEECCEECCCCHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHCCCC
ADAVFNTLNGDSNVAFFRQYNSLGFNADTLPVMSVSIAEEEVGGIGTANIEGQLVAWDYY
CCCEEEECCCCCCEEEEEECCCCCCCCCCCEEEEEEECHHHHCCCEECCCCCEEEEEEHH
QTIDTPENETFVENFKDLYGQDKVTSDPMEAAYTSLYLWKEMVEKADSFDVAAIQAAADG
ECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
TTFDAPEGTVVVDGDNHHISKTPRIGRIRPDGLIDTIWETDSPVDPDPYLSSYDWAKTTA
CCCCCCCCEEEEECCCCEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCC
ATS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907262; 10984043; 1762155; 7813419; 10508151; 10708652 [H]