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The map label for this gene is malE [H]

Identifier: 145294970

GI number: 145294970

Start: 1007982

End: 1009244

Strand: Reverse

Name: malE [H]

Synonym: cgR_0915

Alternate gene names: 145294970

Gene position: 1009244-1007982 (Counterclockwise)

Preceding gene: 145294971

Following gene: 145294969

Centisome position: 30.45

GC content: 66.35

Gene sequence:

>1263_bases
GTGACCTCCCCCCGATTCTCCCGCCGCCAGTTCCTCGGCGCGGCCGGACTGCTCGTCGCGGGTGGAGCACTCACCGCCTG
CGGCAGCGGTGGCGGTCAGGCCGCCGGCTCCGGTGCTCTCCTCGGCAGACCCCGCCCCCTGACCATCTGGGCATCCCAGT
ACGAGGTCCCCGGCCTGGAGAAGGTCGCCGAGCGTTTCCAGGAGGACACGGGTGTGAGCATCCGCGTCATCCAGCGCAAC
TACAACGGCCAGATGCTCTCCGACTTCCTCACCCAGGTCCCCACCGGGGAAGGCCCCGACATCATCGTGGCCCCGCACGA
CGTGCTCGGCCAGGTGGTCAACAACGGTGCCGTATCCCCCGTCGACCTCATCGACCCGGAGGACAACTTCGTCGACATCG
CCCTGCAGGCCGTCACGTACGACGGCCGCTACTACGGCGTGCCCTTCATCATCGAGAACGTCGCCCTCCTGCGGAACAAC
GCGATGACCGATCACACCCCGGAAACCTTCGACGATCTCCTCGCCGAAGGCCACCGGCTCATGGACGCCGGCGTCGCGAA
GTATCCCTTCACCACCAGCCAGTCCGAGGCCTCCGGTGATCCGTACCACCTCTACCCCATCCAGTCCTCCTTCGGCGCCG
AGGTCTTCAAGCGCGACGCCGACGGCGCCTACACCGCCGAACTGGGAATGGGCGGCGACGGGGGCCACGCCTTCGCCAAT
TACCTCGCCGAGATGGGGGCCAACCGCGACCTCATCGTCACGATGACCCCGGACATCTCCAAGCAGGCCTTCATCGACGG
CGAATCCCCCTACTTCATCGCCGGCCCCTGGAACCTGCCGGACATTCAGGCCGCTGACATGGACATCGAGGTTCTCTCCG
TGCCCTCAGCCGGTGGGCAGCCCGCGGTCCCGTTCGTCGGCGTGTCCTCCTTCATGATCAACGCCAACTCCTCCTCCCCG
CTCGCGGCGAGGGACCTGGCGCTCAACTACCTGTCGCAGCCCGAGGTCCAGCTCGAGCTGTTCTCCAACAACCAACGTCC
CCCCGCCAATAAGGAGGCACTCGAGAGCATGGGGGACCCTGTGCTCGCCGGGTACGCCCGCATCGCCCAGGAGGACGGCG
CCCCCATGCCGTCCATTCCGCAGATGGGCGCAGTGTGGAACTTCTGGGGCATCACACAGAACGGCATCGTCAACGGTGCC
GACGACCCCGAGCAACTATGGGACACCATGATCACCAACATCGAGAGGGCGATTGAATCGTGA

Upstream 100 bases:

>100_bases
CCGGCGGCGACACCATGGACCTGTGGTTCACGCCCGAGTCCCTGTACCTCTTCGACGGCGAGACCGGCGAGTCGCTGCGC
ACCGATGAGGTGCGTGCCCA

Downstream 100 bases:

>100_bases
CCACCACACTGGACTCCCGCCCCGTGGCGGATGAGGCGCCGGCCCCGCGGCGTCGTCAAGCAGGAGCCGGTCTGGCGATC
AAGCTCCTCATCATGGCGGC

Product: hypothetical protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: MMBP; Maltodextrin-binding protein [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MTSPRFSRRQFLGAAGLLVAGGALTACGSGGGQAAGSGALLGRPRPLTIWASQYEVPGLEKVAERFQEDTGVSIRVIQRN
YNGQMLSDFLTQVPTGEGPDIIVAPHDVLGQVVNNGAVSPVDLIDPEDNFVDIALQAVTYDGRYYGVPFIIENVALLRNN
AMTDHTPETFDDLLAEGHRLMDAGVAKYPFTTSQSEASGDPYHLYPIQSSFGAEVFKRDADGAYTAELGMGGDGGHAFAN
YLAEMGANRDLIVTMTPDISKQAFIDGESPYFIAGPWNLPDIQAADMDIEVLSVPSAGGQPAVPFVGVSSFMINANSSSP
LAARDLALNYLSQPEVQLELFSNNQRPPANKEALESMGDPVLAGYARIAQEDGAPMPSIPQMGAVWNFWGITQNGIVNGA
DDPEQLWDTMITNIERAIES

Sequences:

>Translated_420_residues
MTSPRFSRRQFLGAAGLLVAGGALTACGSGGGQAAGSGALLGRPRPLTIWASQYEVPGLEKVAERFQEDTGVSIRVIQRN
YNGQMLSDFLTQVPTGEGPDIIVAPHDVLGQVVNNGAVSPVDLIDPEDNFVDIALQAVTYDGRYYGVPFIIENVALLRNN
AMTDHTPETFDDLLAEGHRLMDAGVAKYPFTTSQSEASGDPYHLYPIQSSFGAEVFKRDADGAYTAELGMGGDGGHAFAN
YLAEMGANRDLIVTMTPDISKQAFIDGESPYFIAGPWNLPDIQAADMDIEVLSVPSAGGQPAVPFVGVSSFMINANSSSP
LAARDLALNYLSQPEVQLELFSNNQRPPANKEALESMGDPVLAGYARIAQEDGAPMPSIPQMGAVWNFWGITQNGIVNGA
DDPEQLWDTMITNIERAIES
>Mature_419_residues
TSPRFSRRQFLGAAGLLVAGGALTACGSGGGQAAGSGALLGRPRPLTIWASQYEVPGLEKVAERFQEDTGVSIRVIQRNY
NGQMLSDFLTQVPTGEGPDIIVAPHDVLGQVVNNGAVSPVDLIDPEDNFVDIALQAVTYDGRYYGVPFIIENVALLRNNA
MTDHTPETFDDLLAEGHRLMDAGVAKYPFTTSQSEASGDPYHLYPIQSSFGAEVFKRDADGAYTAELGMGGDGGHAFANY
LAEMGANRDLIVTMTPDISKQAFIDGESPYFIAGPWNLPDIQAADMDIEVLSVPSAGGQPAVPFVGVSSFMINANSSSPL
AARDLALNYLSQPEVQLELFSNNQRPPANKEALESMGDPVLAGYARIAQEDGAPMPSIPQMGAVWNFWGITQNGIVNGAD
DPEQLWDTMITNIERAIES

Specific function: Involved in the high-affinity maltose membrane transport system malEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=353, Percent_Identity=29.4617563739377, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 44862; Mature: 44730

Theoretical pI: Translated: 4.03; Mature: 4.03

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPRFSRRQFLGAAGLLVAGGALTACGSGGGQAAGSGALLGRPRPLTIWASQYEVPGLE
CCCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHH
KVAERFQEDTGVSIRVIQRNYNGQMLSDFLTQVPTGEGPDIIVAPHDVLGQVVNNGAVSP
HHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCCC
VDLIDPEDNFVDIALQAVTYDGRYYGVPFIIENVALLRNNAMTDHTPETFDDLLAEGHRL
EECCCCCCCEEEEEEEEEEECCEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHH
MDAGVAKYPFTTSQSEASGDPYHLYPIQSSFGAEVFKRDADGAYTAELGMGGDGGHAFAN
HHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHCCCCCCEEEECCCCCCCHHHHHH
YLAEMGANRDLIVTMTPDISKQAFIDGESPYFIAGPWNLPDIQAADMDIEVLSVPSAGGQ
HHHHHCCCCCEEEEECCCCCCCEEECCCCCEEEECCCCCCCCEECCCCEEEEECCCCCCC
PAVPFVGVSSFMINANSSSPLAARDLALNYLSQPEVQLELFSNNQRPPANKEALESMGDP
CCCCEEECEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCCH
VLAGYARIAQEDGAPMPSIPQMGAVWNFWGITQNGIVNGADDPEQLWDTMITNIERAIES
HHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSPRFSRRQFLGAAGLLVAGGALTACGSGGGQAAGSGALLGRPRPLTIWASQYEVPGLE
CCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHH
KVAERFQEDTGVSIRVIQRNYNGQMLSDFLTQVPTGEGPDIIVAPHDVLGQVVNNGAVSP
HHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCCC
VDLIDPEDNFVDIALQAVTYDGRYYGVPFIIENVALLRNNAMTDHTPETFDDLLAEGHRL
EECCCCCCCEEEEEEEEEEECCEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHH
MDAGVAKYPFTTSQSEASGDPYHLYPIQSSFGAEVFKRDADGAYTAELGMGGDGGHAFAN
HHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHCCCCCCEEEECCCCCCCHHHHHH
YLAEMGANRDLIVTMTPDISKQAFIDGESPYFIAGPWNLPDIQAADMDIEVLSVPSAGGQ
HHHHHCCCCCEEEEECCCCCCCEEECCCCCEEEECCCCCCCCEECCCCEEEEECCCCCCC
PAVPFVGVSSFMINANSSSPLAARDLALNYLSQPEVQLELFSNNQRPPANKEALESMGDP
CCCCEEECEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCCH
VLAGYARIAQEDGAPMPSIPQMGAVWNFWGITQNGIVNGADDPEQLWDTMITNIERAIES
HHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]