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The map label for this gene is prfB

Identifier: 145294961

GI number: 145294961

Start: 1000122

End: 1001228

Strand: Direct

Name: prfB

Synonym: cgR_0906

Alternate gene names: 145294961

Gene position: 1000122-1001228 (Clockwise)

Preceding gene: 145294956

Following gene: 145294962

Centisome position: 30.18

GC content: 55.47

Gene sequence:

>1107_bases
ATGCGTCCCGAATTTTCTGCAGAACTCTCCGAGCTAGACAGCACGCTGACAACCATTGAAAAAGTGCTGAACCCGCAAGA
GATGTCTGACCGAGTCAGAGAACTTGAAGCTCAAGCAGCTGACCCTTCTCTGTGGGATGACCCTGACCATGCACAGCAAG
TCACCTCTGAGCTGTCCCACGTCCAGGCAGAGCTGCGCAAAATCACCGATCTGCGCCAGCGCATCGAAGATCTGCCCATC
ATGGTGGAACTCGCAGAGGAAGAAGACGGCGATACCTCCATCGCGGAAGAAGAACTCGCCGATCTGCGTTCTCTGATCGA
TGCGTTGGAAGTAAAGACCATGCTGTCGGGTGAATATGATGCTCGCGAGGCAGTGATCAATATTCGATCCGGTGCCGGTG
GTGTCGATGCTGCGGACTGGGCTGAAATGCTCATGCGCATGTACACCCGCTGGGCGGAAAAGAACGGCCACAAAGTAGAT
ATTTACGATATTTCCTACGCCGAAGAAGCCGGCATCAAATCCGCCACCTTCGTGGTCCACGGCGACTACATGTACGGCCA
GCTCTCCGTGGAGCAAGGCGCACACCGCCTCGTGCGCATCAGTCCTTTTGATAACCAGGGCAGGCGCCAAACCTCCTTCG
CCGAGGTAGAAGTTCTTCCCGTGGTGGAAAAAGTGGACTCCATCGACATCCCTGATGCCGATGTTCGCGTCGATGTCTAC
CGCTCCTCCGGCCCAGGTGGTCAGTCCGTGAACACCACCGACTCTGCCGTGCGCCTGACCCACATCCCAACCGGCATCGT
GGTGACCTGCCAAAACGAGAAATCACAGATCCAAAACAAGGCATCCGCGATGCGTGTTCTCCAGGCAAAACTGCTTGAGC
GTAAACGCCAGGAAGAACGCGCCGAAATGGATGCCCTCGGAGCTGGAGGCAATGCATCCTGGGGTAACCAAATGCGTTCC
TACGTGCTGCACCCTTATCAAATGGTGAAGGATCTGCGCACCAACTTTGAAGTCAACGATCCGCAAAAAGTCCTTGACGG
CGATATCGATGGCCTTTTGGAAGCAGGTATTCGCTGGCGAATGGCTGAGAGCCAGTCGGCGGAATAA

Upstream 100 bases:

>100_bases
CTGGATTAGTCATGTCTTCCATTGTGCATGCCGTCGGCCAAGTGTGCTGAATAAGGTGGCGTTCTAAGAATCACAATCGG
TGCGGGTAATATGTGCAATC

Downstream 100 bases:

>100_bases
AGGTTGGTTTTCTGGCAGAATCTGGCGATTTCTTAAGAATTACCGGTTATTTGTCGTTGAGTTCTCCTCTGAGCACTCAG
GGTAGTCACTTCCGTTTCTT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MRPEFSAELSELDSTLTTIEKVLNPQEMSDRVRELEAQAADPSLWDDPDHAQQVTSELSHVQAELRKITDLRQRIEDLPI
MVELAEEEDGDTSIAEEELADLRSLIDALEVKTMLSGEYDAREAVINIRSGAGGVDAADWAEMLMRMYTRWAEKNGHKVD
IYDISYAEEAGIKSATFVVHGDYMYGQLSVEQGAHRLVRISPFDNQGRRQTSFAEVEVLPVVEKVDSIDIPDADVRVDVY
RSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQIQNKASAMRVLQAKLLERKRQEERAEMDALGAGGNASWGNQMRS
YVLHPYQMVKDLRTNFEVNDPQKVLDGDIDGLLEAGIRWRMAESQSAE

Sequences:

>Translated_368_residues
MRPEFSAELSELDSTLTTIEKVLNPQEMSDRVRELEAQAADPSLWDDPDHAQQVTSELSHVQAELRKITDLRQRIEDLPI
MVELAEEEDGDTSIAEEELADLRSLIDALEVKTMLSGEYDAREAVINIRSGAGGVDAADWAEMLMRMYTRWAEKNGHKVD
IYDISYAEEAGIKSATFVVHGDYMYGQLSVEQGAHRLVRISPFDNQGRRQTSFAEVEVLPVVEKVDSIDIPDADVRVDVY
RSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQIQNKASAMRVLQAKLLERKRQEERAEMDALGAGGNASWGNQMRS
YVLHPYQMVKDLRTNFEVNDPQKVLDGDIDGLLEAGIRWRMAESQSAE
>Mature_368_residues
MRPEFSAELSELDSTLTTIEKVLNPQEMSDRVRELEAQAADPSLWDDPDHAQQVTSELSHVQAELRKITDLRQRIEDLPI
MVELAEEEDGDTSIAEEELADLRSLIDALEVKTMLSGEYDAREAVINIRSGAGGVDAADWAEMLMRMYTRWAEKNGHKVD
IYDISYAEEAGIKSATFVVHGDYMYGQLSVEQGAHRLVRISPFDNQGRRQTSFAEVEVLPVVEKVDSIDIPDADVRVDVY
RSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQIQNKASAMRVLQAKLLERKRQEERAEMDALGAGGNASWGNQMRS
YVLHPYQMVKDLRTNFEVNDPQKVLDGDIDGLLEAGIRWRMAESQSAE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=271, Percent_Identity=32.8413284132841, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI34577120, Length=308, Percent_Identity=31.1688311688312, Blast_Score=131, Evalue=8e-31,
Organism=Homo sapiens, GI166795305, Length=182, Percent_Identity=32.4175824175824, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI2367172, Length=332, Percent_Identity=46.0843373493976, Blast_Score=291, Evalue=3e-80,
Organism=Escherichia coli, GI1787462, Length=253, Percent_Identity=40.3162055335968, Blast_Score=180, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17542784, Length=234, Percent_Identity=32.0512820512821, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321295, Length=320, Percent_Identity=32.5, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19921226, Length=239, Percent_Identity=32.6359832635983, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_CORGB (A4QCD3)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001137782.1
- ProteinModelPortal:   A4QCD3
- SMR:   A4QCD3
- STRING:   A4QCD3
- GeneID:   4992259
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_0906
- eggNOG:   COG1186
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- ProtClustDB:   PRK00578
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41137; Mature: 41137

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPEFSAELSELDSTLTTIEKVLNPQEMSDRVRELEAQAADPSLWDDPDHAQQVTSELSH
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
VQAELRKITDLRQRIEDLPIMVELAEEEDGDTSIAEEELADLRSLIDALEVKTMLSGEYD
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AREAVINIRSGAGGVDAADWAEMLMRMYTRWAEKNGHKVDIYDISYAEEAGIKSATFVVH
CHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCCCEEEEEEE
GDYMYGQLSVEQGAHRLVRISPFDNQGRRQTSFAEVEVLPVVEKVDSIDIPDADVRVDVY
CCEEEEEEEHHHCCCEEEEECCCCCCCCCCCCHHHEEEHHHHHHHCCCCCCCCCEEEEEE
RSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQIQNKASAMRVLQAKLLERKRQEER
ECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEMDALGAGGNASWGNQMRSYVLHPYQMVKDLRTNFEVNDPQKVLDGDIDGLLEAGIRWR
HHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHCCCEE
MAESQSAE
ECCCCCCC
>Mature Secondary Structure
MRPEFSAELSELDSTLTTIEKVLNPQEMSDRVRELEAQAADPSLWDDPDHAQQVTSELSH
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
VQAELRKITDLRQRIEDLPIMVELAEEEDGDTSIAEEELADLRSLIDALEVKTMLSGEYD
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AREAVINIRSGAGGVDAADWAEMLMRMYTRWAEKNGHKVDIYDISYAEEAGIKSATFVVH
CHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCCCEEEEEEE
GDYMYGQLSVEQGAHRLVRISPFDNQGRRQTSFAEVEVLPVVEKVDSIDIPDADVRVDVY
CCEEEEEEEHHHCCCEEEEECCCCCCCCCCCCHHHEEEHHHHHHHCCCCCCCCCEEEEEE
RSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQIQNKASAMRVLQAKLLERKRQEER
ECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEMDALGAGGNASWGNQMRSYVLHPYQMVKDLRTNFEVNDPQKVLDGDIDGLLEAGIRWR
HHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHCCCEE
MAESQSAE
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA