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The map label for this gene is pdxT

Identifier: 145294952

GI number: 145294952

Start: 992222

End: 992824

Strand: Direct

Name: pdxT

Synonym: cgR_0898

Alternate gene names: 145294952

Gene position: 992222-992824 (Clockwise)

Preceding gene: 145294951

Following gene: 145294954

Centisome position: 29.94

GC content: 56.05

Gene sequence:

>603_bases
GTGATCGTTGGAGTTTTAGCTCTCCAAGGCGGGGTGGAAGAACACCTCACCGCCTTGGAAGCTCTCGGAGCGACAACCCG
AAAAGTACGTGTGCCAAAGGACCTTGATGGTCTCGAAGGCATCGTCATTCCCGGCGGGGAATCCACCGTGTTGGACAAAC
TAGCTCGGACATTCGACGTGGCAGAACCTCTAGCGAATCTCATTCGCGACGGCCTACCCGTTTTCGCTACCTGCGCTGGC
CTGATCTATCTGGCGAAACACCTCGACAACCCAGCAAGGGGACAGCAGACGTTGGAATTGCTGGACGTGGTGGTGCGTCG
AAACGCATTCGGCACCCAACGCGAATCCTTCGACACCACCGTGGATGTTTCCTTCGACGGTGCAACATTCCCCGGAGTGC
AGGCCTCGTTTATCCGAGCTCCCATCGTCACTGCTTTTGGTCCTACGGTAGAAGCGATCGCTGCTCTCAACGGTGGGGAG
GTGGTTGGTGTACGCCAAGGCAACATCATCGCGCTGTCTTTCCATCCCGAAGAAACCGGCGATTACCGCATTCACCAAGC
TTGGCTGAACCTGATTGGAAAACGTACTGAACTGGCGATTTGA

Upstream 100 bases:

>100_bases
CCTCCGTCATCGCCGACGTATCCCGCGGCCTCGGTGAAGCCATGGTGGGCATCAACGTATCCGACGTCCCAGCACCACAC
CGACTCGCCGAGCGCGGCTG

Downstream 100 bases:

>100_bases
TGTTTGTAGTAGCGCTCTGTATAGTTTCTACTCGTTGCACAGAGCGCGAAAGCAATCATTGCAATAGATTAGATGGTTTC
ACAATCATCGCGTCGCGGGG

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MIVGVLALQGGVEEHLTALEALGATTRKVRVPKDLDGLEGIVIPGGESTVLDKLARTFDVAEPLANLIRDGLPVFATCAG
LIYLAKHLDNPARGQQTLELLDVVVRRNAFGTQRESFDTTVDVSFDGATFPGVQASFIRAPIVTAFGPTVEAIAALNGGE
VVGVRQGNIIALSFHPEETGDYRIHQAWLNLIGKRTELAI

Sequences:

>Translated_200_residues
MIVGVLALQGGVEEHLTALEALGATTRKVRVPKDLDGLEGIVIPGGESTVLDKLARTFDVAEPLANLIRDGLPVFATCAG
LIYLAKHLDNPARGQQTLELLDVVVRRNAFGTQRESFDTTVDVSFDGATFPGVQASFIRAPIVTAFGPTVEAIAALNGGE
VVGVRQGNIIALSFHPEETGDYRIHQAWLNLIGKRTELAI
>Mature_200_residues
MIVGVLALQGGVEEHLTALEALGATTRKVRVPKDLDGLEGIVIPGGESTVLDKLARTFDVAEPLANLIRDGLPVFATCAG
LIYLAKHLDNPARGQQTLELLDVVVRRNAFGTQRESFDTTVDVSFDGATFPGVQASFIRAPIVTAFGPTVEAIAALNGGE
VVGVRQGNIIALSFHPEETGDYRIHQAWLNLIGKRTELAI

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=207, Percent_Identity=36.7149758454106, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321048, Length=210, Percent_Identity=35.7142857142857, Blast_Score=101, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6323995, Length=210, Percent_Identity=34.7619047619048, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_CORGB (A4QCC4)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001137773.1
- ProteinModelPortal:   A4QCC4
- SMR:   A4QCC4
- STRING:   A4QCC4
- GeneID:   4994331
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_0898
- eggNOG:   COG0311
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- ProtClustDB:   PRK13525
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 21324; Mature: 21324

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 78-78 ACT_SITE 175-175 ACT_SITE 177-177 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVGVLALQGGVEEHLTALEALGATTRKVRVPKDLDGLEGIVIPGGESTVLDKLARTFDV
CEEEEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHH
AEPLANLIRDGLPVFATCAGLIYLAKHLDNPARGQQTLELLDVVVRRNAFGTQRESFDTT
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCE
VDVSFDGATFPGVQASFIRAPIVTAFGPTVEAIAALNGGEVVGVRQGNIIALSFHPEETG
EEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEECCCEEEEEECCCCCC
DYRIHQAWLNLIGKRTELAI
CCHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MIVGVLALQGGVEEHLTALEALGATTRKVRVPKDLDGLEGIVIPGGESTVLDKLARTFDV
CEEEEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHH
AEPLANLIRDGLPVFATCAGLIYLAKHLDNPARGQQTLELLDVVVRRNAFGTQRESFDTT
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCE
VDVSFDGATFPGVQASFIRAPIVTAFGPTVEAIAALNGGEVVGVRQGNIIALSFHPEETG
EEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEECCCEEEEEECCCCCC
DYRIHQAWLNLIGKRTELAI
CCHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA