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The map label for this gene is rffG [C]

Identifier: 145294730

GI number: 145294730

Start: 737501

End: 738454

Strand: Reverse

Name: rffG [C]

Synonym: cgR_0678

Alternate gene names: 145294730

Gene position: 738454-737501 (Counterclockwise)

Preceding gene: 145294739

Following gene: 145294729

Centisome position: 22.28

GC content: 53.35

Gene sequence:

>954_bases
ATGCGTTGCGTTATCACCGGTGGTGCTGGATTCCTAGGCAGCCATCTGACTGACCTGATTCTCAATCAAGGGCACGAAGT
TATCGTTCTGGACGACCTCAGCACAGGATCACTCAGCAACCTCTTCCACCAAATTAGTAACCCTCGCCTGCAGATCAAGA
CTGTCGATGTGAGGAAAAAGTTCGAAATCGATGGACCAGTCGATATCGTATTCAACCTCGCCAGCCCAGCGTCTCCCCCT
GTTTACACTCAGCGTCGTGTTGAATGCCTCCTTATTAACTCAGAGGCAGTACTCCAAGTCGCAGAGTTCGCCTTGGAAAA
GGGCGCTCGTCTCGTACAGGCCTCAACAAGTGAGGTATACGGAGATCCACTGAGCCACCCCCAACTCGAACACCACTGGG
GCAATGTCAACCCCATCGGTGAGCGCAGCTGCTATGACGAAGGCAAGCGATTCGCCGAAGCACTCCTCTCTGCAATGCGA
CTGGAGCAAGGACTCAACGCGGGCATCATTCGAATCTTCAACACCTATGGGCCTCGCATGCACCCCTTTGATGGTCGAGT
GATTTCCGGGTTTGTGCGCCAGGCCTTGGCCAATGAGCCACTCACCGTTTTCGGAGATGGATCTCAAACACGCAGCTTCT
GCTATGTCTCAGACCTCGTTCGAGGCCTGTGGCTCATGGGTAATTCAAACCAGCCCGGCCCCATCAACCTGGGTAATCCA
ATTGAACAAACGGTTCTGAGCATGGCCCACTTGATTAAGGAATCCACTAATTCCGAATCCTCGATCACTTTTGAGCCTCT
ACCTTCGGATGATCCAGTACGTCGTCGTCCCGACATTTCCAAAGCCAAGGAGCTCCTCGGTTGGGAGCCTCTTGTTGGCA
TCGACGTGGGCCTTCGCGAAGTCATCAATTGGCAGATCGAGACGCAAAGGACGTACGCGGAGAAGATATCGTGA

Upstream 100 bases:

>100_bases
AAACTGCATATCCATACCCAAAAACCACATTTCTTAAAGGAATCAAAACTGTTCCTTAAGTATTGATGAGAAGTTGATTA
TTTTTCTGGAAGGATTCAAA

Downstream 100 bases:

>100_bases
CTGTCCGACGAGCTGGGACTCGCCAAGCAAGAGTGAGGCACCAGCCTCTCCATAAAAAGTCCCAGAATCTCATCCCTTCG
GATCTATCTGCAGAAATTCA

Product: hypothetical protein

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MRCVITGGAGFLGSHLTDLILNQGHEVIVLDDLSTGSLSNLFHQISNPRLQIKTVDVRKKFEIDGPVDIVFNLASPASPP
VYTQRRVECLLINSEAVLQVAEFALEKGARLVQASTSEVYGDPLSHPQLEHHWGNVNPIGERSCYDEGKRFAEALLSAMR
LEQGLNAGIIRIFNTYGPRMHPFDGRVISGFVRQALANEPLTVFGDGSQTRSFCYVSDLVRGLWLMGNSNQPGPINLGNP
IEQTVLSMAHLIKESTNSESSITFEPLPSDDPVRRRPDISKAKELLGWEPLVGIDVGLREVINWQIETQRTYAEKIS

Sequences:

>Translated_317_residues
MRCVITGGAGFLGSHLTDLILNQGHEVIVLDDLSTGSLSNLFHQISNPRLQIKTVDVRKKFEIDGPVDIVFNLASPASPP
VYTQRRVECLLINSEAVLQVAEFALEKGARLVQASTSEVYGDPLSHPQLEHHWGNVNPIGERSCYDEGKRFAEALLSAMR
LEQGLNAGIIRIFNTYGPRMHPFDGRVISGFVRQALANEPLTVFGDGSQTRSFCYVSDLVRGLWLMGNSNQPGPINLGNP
IEQTVLSMAHLIKESTNSESSITFEPLPSDDPVRRRPDISKAKELLGWEPLVGIDVGLREVINWQIETQRTYAEKIS
>Mature_317_residues
MRCVITGGAGFLGSHLTDLILNQGHEVIVLDDLSTGSLSNLFHQISNPRLQIKTVDVRKKFEIDGPVDIVFNLASPASPP
VYTQRRVECLLINSEAVLQVAEFALEKGARLVQASTSEVYGDPLSHPQLEHHWGNVNPIGERSCYDEGKRFAEALLSAMR
LEQGLNAGIIRIFNTYGPRMHPFDGRVISGFVRQALANEPLTVFGDGSQTRSFCYVSDLVRGLWLMGNSNQPGPINLGNP
IEQTVLSMAHLIKESTNSESSITFEPLPSDDPVRRRPDISKAKELLGWEPLVGIDVGLREVINWQIETQRTYAEKIS

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=303, Percent_Identity=52.1452145214521, Blast_Score=313, Evalue=1e-85,
Organism=Homo sapiens, GI7657641, Length=325, Percent_Identity=29.8461538461538, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI48994969, Length=343, Percent_Identity=26.2390670553936, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788589, Length=340, Percent_Identity=23.8235294117647, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788353, Length=351, Percent_Identity=25.0712250712251, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786974, Length=349, Percent_Identity=23.4957020057307, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17539532, Length=304, Percent_Identity=46.3815789473684, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI115532424, Length=328, Percent_Identity=28.6585365853659, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17568069, Length=329, Percent_Identity=26.4437689969605, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71982035, Length=352, Percent_Identity=23.5795454545455, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21356223, Length=303, Percent_Identity=48.1848184818482, Blast_Score=293, Evalue=7e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 35070; Mature: 35070

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCVITGGAGFLGSHLTDLILNQGHEVIVLDDLSTGSLSNLFHQISNPRLQIKTVDVRKK
CEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCEEE
FEIDGPVDIVFNLASPASPPVYTQRRVECLLINSEAVLQVAEFALEKGARLVQASTSEVY
ECCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCEEECCCHHHC
GDPLSHPQLEHHWGNVNPIGERSCYDEGKRFAEALLSAMRLEQGLNAGIIRIFNTYGPRM
CCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
HPFDGRVISGFVRQALANEPLTVFGDGSQTRSFCYVSDLVRGLWLMGNSNQPGPINLGNP
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCH
IEQTVLSMAHLIKESTNSESSITFEPLPSDDPVRRRPDISKAKELLGWEPLVGIDVGLRE
HHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCEECHHHHHH
VINWQIETQRTYAEKIS
HHCCEEEHHHHHHHHCC
>Mature Secondary Structure
MRCVITGGAGFLGSHLTDLILNQGHEVIVLDDLSTGSLSNLFHQISNPRLQIKTVDVRKK
CEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCEEE
FEIDGPVDIVFNLASPASPPVYTQRRVECLLINSEAVLQVAEFALEKGARLVQASTSEVY
ECCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCEEECCCHHHC
GDPLSHPQLEHHWGNVNPIGERSCYDEGKRFAEALLSAMRLEQGLNAGIIRIFNTYGPRM
CCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
HPFDGRVISGFVRQALANEPLTVFGDGSQTRSFCYVSDLVRGLWLMGNSNQPGPINLGNP
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCH
IEQTVLSMAHLIKESTNSESSITFEPLPSDDPVRRRPDISKAKELLGWEPLVGIDVGLRE
HHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCEECHHHHHH
VINWQIETQRTYAEKIS
HHCCEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]