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The map label for this gene is ptrB [C]

Identifier: 145294468

GI number: 145294468

Start: 462124

End: 464139

Strand: Reverse

Name: ptrB [C]

Synonym: cgR_0423

Alternate gene names: 145294468

Gene position: 464139-462124 (Counterclockwise)

Preceding gene: 145294469

Following gene: 145294467

Centisome position: 14.0

GC content: 57.74

Gene sequence:

>2016_bases
ATGACTGATTACACGTTCCTCGAAGACATTGACACCCCGGAAGCGCTCGCGTGGGCGGAAAAATGGTCGGGGGAAAGCGT
CGAAAAGCTAAAAAGTCCTGATAAAGACGCACTCGAAGCCAGGCTGCTGGCTGCGTTGGACACCGATGACCGTATTGCGT
ACGTGAGCAGGCGCGGGGAGAAGCTGTATAACTTTTGGCGGGATGCGCAGCATCCGCGTGGTGTGTGGCGCACGACCACC
TTGGAGTCCTATTACAGTGCGCAGCCTAAGTGGGACGTGTTAATTGATGTGGATGCGTTGGCGGAGGCCGAGGGCGAAAA
CTGGGTATGGAAGGGCGCGGTGGTGCGTTCGCCGGAGCGGGATTTAGCGCTGGTTAAGTTGTCGCGGGGCGGCGCGGATG
CCACGGTTATTAGGGAGTTTGACCTGGCCACTCGTGAATTCGTGGGGGCTGACGCTTTTGAATTATCTGAGGCCAAGTCC
AGCGTGACGTGGATCGATGCGGACACGCTGCTTGTTGGAACCGACACCGGTGAAGGCTCATTGACGGATTCGGGTTACCC
GGCGCGTGCGGTGACGTGGAAGCGTGGCACCCCGATTGAGCAGGCTGACGTGTTTTTTGAAGGCGCGCGTGAGGATGTTG
CGACTCATGCGTGGCGGGATTCCACCCCTGGGTTTGAACGGATTTTCGCCTCACGTTCCTTAGATTTTTACAACTCGGAA
ACCTATTTAGAGACCGCGGATGGTTTGGTGCTTTTGGAGGTGCCGACTGATTGCGATGTGATCGTGAAGAATCAGTGGCT
GTTTGTAAATCCCCGTACTGATTATGCGGGTATTCTGGCGGGTGGTTTGGGTGTCCTGGAGTTGGATGCGTTCTTAGGTG
GTGCCCGGGATTTCGCTGCGGTGTTCACGCCTTCTGCGTCTACCTCGTTGCAGGGGCTTGCCACCACAAAGAATTTCTTG
GTGCTTACCTTATTAAATAATGTGTCGACAGAAATCGTCTCCGTGCCGCTCGCTGATCCGGTTGCGGAACCGACCCGCAT
GGAGCTTCCCGATCACGTCACCGCGCATGTGGTTGCTACCTCCCCGTTGGATGGCGATGAGATTTGGGTGCAGGCAGCGA
GTTTCACCGAGGCGCCAACGTTGCTGCGTACTGAGCTCCCTGGTGCGCTTGAGGCTGTGAAGAAGGCGCCGTTGCAGTTT
GAAAATGCTGGTCAGGAGACTCGTCAGCATTGGGCGACCTCGGCGGATGGAACGAAGATTCCGTACTTTATTACGGGTAA
CTTTGCCGGGACTTCGGCAGCCCAGCGGAAAACGTTGGTCCACGCCTACGGCGGCTTCGAGGTTTCCCTTACCCCAAGCC
ACTCCCCGACCCGCGGCATCGCATGGTTGGAAAAGGGCTACTACTTTGTGGAAGCCAATCTACGTGGTGGCGGCGAATTC
GGTCCGGAATGGCATTCGCAGGCAACCAAGCTGAACCGCATGAAGGTATGGGAGGATCACCGCGCGGTGCTCGCCGACCT
TGTGGAGCGCGGCTACGCAACCCCGGAGCAGATTGCGATTCGTGGCGGATCCAACGGTGGTTTGCTGACAAGTGGCGCGT
TAACCCAGTACCCAGAGGCCTTTGGTGCGGCAGTTGTACAGGTGCCATTGGCTGATATGTTGCGCTATCACAGCTGGTCA
GCGGGTGCTTCGTGGATGGCGGAGTACGGCAACCCCGACGATCCGGAGGAACGGGCGGTGATTGAGCAATACTCGCCGGT
GCAGGCGGTGGTGGGCGTCGAGAAGCGAATTTATCCACCCGCATTGGTGACAACCTCAACCCGAGACGACCGCGTCCACC
CCGCGCATGCCCGCCTTTTTGCTCAAGCTTTGCTTGATGCGGGCCAAGCCGTGGACTACTACGAAAACACCGAGGGCGGC
CATGCCGGCGCGGCGGATAACAAGCAGACCGCGTTTGTGGAATCGCTGATCTACACCTGGATCGAGAAGACTTTGGATCA
GCAGGGTAGCATTTAA

Upstream 100 bases:

>100_bases
TACTACTCGGTTCAAGTTTACGTCGGCTGATCCATTTGAAGGCGTCCGCAGAAGACGCCTTAAAAAACCTGCCAGGTAAA
AGATCACTACCCTGATCTTC

Downstream 100 bases:

>100_bases
TACCTATGATTATGCGAAGGCTGCGCTCCACCCCGGTCCCTGGTACACGCGATTCCTACACGGGAATTGATTTCAACTTA
GGCTTCCACATCCGACGCTA

Product: hypothetical protein

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 670

Protein sequence:

>671_residues
MTDYTFLEDIDTPEALAWAEKWSGESVEKLKSPDKDALEARLLAALDTDDRIAYVSRRGEKLYNFWRDAQHPRGVWRTTT
LESYYSAQPKWDVLIDVDALAEAEGENWVWKGAVVRSPERDLALVKLSRGGADATVIREFDLATREFVGADAFELSEAKS
SVTWIDADTLLVGTDTGEGSLTDSGYPARAVTWKRGTPIEQADVFFEGAREDVATHAWRDSTPGFERIFASRSLDFYNSE
TYLETADGLVLLEVPTDCDVIVKNQWLFVNPRTDYAGILAGGLGVLELDAFLGGARDFAAVFTPSASTSLQGLATTKNFL
VLTLLNNVSTEIVSVPLADPVAEPTRMELPDHVTAHVVATSPLDGDEIWVQAASFTEAPTLLRTELPGALEAVKKAPLQF
ENAGQETRQHWATSADGTKIPYFITGNFAGTSAAQRKTLVHAYGGFEVSLTPSHSPTRGIAWLEKGYYFVEANLRGGGEF
GPEWHSQATKLNRMKVWEDHRAVLADLVERGYATPEQIAIRGGSNGGLLTSGALTQYPEAFGAAVVQVPLADMLRYHSWS
AGASWMAEYGNPDDPEERAVIEQYSPVQAVVGVEKRIYPPALVTTSTRDDRVHPAHARLFAQALLDAGQAVDYYENTEGG
HAGAADNKQTAFVESLIYTWIEKTLDQQGSI

Sequences:

>Translated_671_residues
MTDYTFLEDIDTPEALAWAEKWSGESVEKLKSPDKDALEARLLAALDTDDRIAYVSRRGEKLYNFWRDAQHPRGVWRTTT
LESYYSAQPKWDVLIDVDALAEAEGENWVWKGAVVRSPERDLALVKLSRGGADATVIREFDLATREFVGADAFELSEAKS
SVTWIDADTLLVGTDTGEGSLTDSGYPARAVTWKRGTPIEQADVFFEGAREDVATHAWRDSTPGFERIFASRSLDFYNSE
TYLETADGLVLLEVPTDCDVIVKNQWLFVNPRTDYAGILAGGLGVLELDAFLGGARDFAAVFTPSASTSLQGLATTKNFL
VLTLLNNVSTEIVSVPLADPVAEPTRMELPDHVTAHVVATSPLDGDEIWVQAASFTEAPTLLRTELPGALEAVKKAPLQF
ENAGQETRQHWATSADGTKIPYFITGNFAGTSAAQRKTLVHAYGGFEVSLTPSHSPTRGIAWLEKGYYFVEANLRGGGEF
GPEWHSQATKLNRMKVWEDHRAVLADLVERGYATPEQIAIRGGSNGGLLTSGALTQYPEAFGAAVVQVPLADMLRYHSWS
AGASWMAEYGNPDDPEERAVIEQYSPVQAVVGVEKRIYPPALVTTSTRDDRVHPAHARLFAQALLDAGQAVDYYENTEGG
HAGAADNKQTAFVESLIYTWIEKTLDQQGSI
>Mature_670_residues
TDYTFLEDIDTPEALAWAEKWSGESVEKLKSPDKDALEARLLAALDTDDRIAYVSRRGEKLYNFWRDAQHPRGVWRTTTL
ESYYSAQPKWDVLIDVDALAEAEGENWVWKGAVVRSPERDLALVKLSRGGADATVIREFDLATREFVGADAFELSEAKSS
VTWIDADTLLVGTDTGEGSLTDSGYPARAVTWKRGTPIEQADVFFEGAREDVATHAWRDSTPGFERIFASRSLDFYNSET
YLETADGLVLLEVPTDCDVIVKNQWLFVNPRTDYAGILAGGLGVLELDAFLGGARDFAAVFTPSASTSLQGLATTKNFLV
LTLLNNVSTEIVSVPLADPVAEPTRMELPDHVTAHVVATSPLDGDEIWVQAASFTEAPTLLRTELPGALEAVKKAPLQFE
NAGQETRQHWATSADGTKIPYFITGNFAGTSAAQRKTLVHAYGGFEVSLTPSHSPTRGIAWLEKGYYFVEANLRGGGEFG
PEWHSQATKLNRMKVWEDHRAVLADLVERGYATPEQIAIRGGSNGGLLTSGALTQYPEAFGAAVVQVPLADMLRYHSWSA
GASWMAEYGNPDDPEERAVIEQYSPVQAVVGVEKRIYPPALVTTSTRDDRVHPAHARLFAQALLDAGQAVDYYENTEGGH
AGAADNKQTAFVESLIYTWIEKTLDQQGSI

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=331, Percent_Identity=30.5135951661631, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI284172438, Length=205, Percent_Identity=25.3658536585366, Blast_Score=81, Evalue=4e-15,
Organism=Homo sapiens, GI284172431, Length=205, Percent_Identity=25.3658536585366, Blast_Score=81, Evalue=4e-15,
Organism=Homo sapiens, GI284172420, Length=205, Percent_Identity=25.3658536585366, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI284172413, Length=205, Percent_Identity=25.3658536585366, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI70778815, Length=205, Percent_Identity=25.3658536585366, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI108860686, Length=285, Percent_Identity=24.2105263157895, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI108860692, Length=201, Percent_Identity=24.8756218905473, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI1788150, Length=327, Percent_Identity=25.3822629969419, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24583414, Length=317, Percent_Identity=32.807570977918, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI221510989, Length=303, Percent_Identity=35.3135313531353, Blast_Score=166, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 73511; Mature: 73380

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDYTFLEDIDTPEALAWAEKWSGESVEKLKSPDKDALEARLLAALDTDDRIAYVSRRGE
CCCCCCHHCCCCCHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCEEHHHHCCH
KLYNFWRDAQHPRGVWRTTTLESYYSAQPKWDVLIDVDALAEAEGENWVWKGAVVRSPER
HHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCEEEEEHHHHHCCCCCCEEEECCEEECCCC
DLALVKLSRGGADATVIREFDLATREFVGADAFELSEAKSSVTWIDADTLLVGTDTGEGS
CEEEEEEECCCCCEEEEEEHHHHHHHHCCCCHHHHHHCCCCEEEEECCEEEEECCCCCCC
LTDSGYPARAVTWKRGTPIEQADVFFEGAREDVATHAWRDSTPGFERIFASRSLDFYNSE
CCCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCC
TYLETADGLVLLEVPTDCDVIVKNQWLFVNPRTDYAGILAGGLGVLELDAFLGGARDFAA
CEEECCCCEEEEECCCCCCEEEECCEEEECCCCCCHHHHHCCCCCEEHHHHHCCCCCEEE
VFTPSASTSLQGLATTKNFLVLTLLNNVSTEIVSVPLADPVAEPTRMELPDHVTAHVVAT
EECCCCCCCCCCCCCCCCEEEEEEHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEE
SPLDGDEIWVQAASFTEAPTLLRTELPGALEAVKKAPLQFENAGQETRQHWATSADGTKI
CCCCCCCEEEEECCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCC
PYFITGNFAGTSAAQRKTLVHAYGGFEVSLTPSHSPTRGIAWLEKGYYFVEANLRGGGEF
CEEEECCCCCCCHHHHCEEEEEECCEEEEECCCCCCCCCHHEEECCEEEEEEEECCCCCC
GPEWHSQATKLNRMKVWEDHRAVLADLVERGYATPEQIAIRGGSNGGLLTSGALTQYPEA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEECCCCCEEECCCHHHCHHH
FGAAVVQVPLADMLRYHSWSAGASWMAEYGNPDDPEERAVIEQYSPVQAVVGVEKRIYPP
HCCCEEEECHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCC
ALVTTSTRDDRVHPAHARLFAQALLDAGQAVDYYENTEGGHAGAADNKQTAFVESLIYTW
EEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
IEKTLDQQGSI
HHHHHHHCCCC
>Mature Secondary Structure 
TDYTFLEDIDTPEALAWAEKWSGESVEKLKSPDKDALEARLLAALDTDDRIAYVSRRGE
CCCCCHHCCCCCHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCEEHHHHCCH
KLYNFWRDAQHPRGVWRTTTLESYYSAQPKWDVLIDVDALAEAEGENWVWKGAVVRSPER
HHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCEEEEEHHHHHCCCCCCEEEECCEEECCCC
DLALVKLSRGGADATVIREFDLATREFVGADAFELSEAKSSVTWIDADTLLVGTDTGEGS
CEEEEEEECCCCCEEEEEEHHHHHHHHCCCCHHHHHHCCCCEEEEECCEEEEECCCCCCC
LTDSGYPARAVTWKRGTPIEQADVFFEGAREDVATHAWRDSTPGFERIFASRSLDFYNSE
CCCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCC
TYLETADGLVLLEVPTDCDVIVKNQWLFVNPRTDYAGILAGGLGVLELDAFLGGARDFAA
CEEECCCCEEEEECCCCCCEEEECCEEEECCCCCCHHHHHCCCCCEEHHHHHCCCCCEEE
VFTPSASTSLQGLATTKNFLVLTLLNNVSTEIVSVPLADPVAEPTRMELPDHVTAHVVAT
EECCCCCCCCCCCCCCCCEEEEEEHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEE
SPLDGDEIWVQAASFTEAPTLLRTELPGALEAVKKAPLQFENAGQETRQHWATSADGTKI
CCCCCCCEEEEECCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCC
PYFITGNFAGTSAAQRKTLVHAYGGFEVSLTPSHSPTRGIAWLEKGYYFVEANLRGGGEF
CEEEECCCCCCCHHHHCEEEEEECCEEEEECCCCCCCCCHHEEECCEEEEEEEECCCCCC
GPEWHSQATKLNRMKVWEDHRAVLADLVERGYATPEQIAIRGGSNGGLLTSGALTQYPEA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEECCCCCEEECCCHHHCHHH
FGAAVVQVPLADMLRYHSWSAGASWMAEYGNPDDPEERAVIEQYSPVQAVVGVEKRIYPP
HCCCEEEECHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCC
ALVTTSTRDDRVHPAHARLFAQALLDAGQAVDYYENTEGGHAGAADNKQTAFVESLIYTW
EEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
IEKTLDQQGSI
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.47 {tosyl-Lys-methyl} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]