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The map label for this gene is leuA

Identifier: 145294368

GI number: 145294368

Start: 357357

End: 359207

Strand: Reverse

Name: leuA

Synonym: cgR_0323

Alternate gene names: 145294368

Gene position: 359207-357357 (Counterclockwise)

Preceding gene: 145294371

Following gene: 145294367

Centisome position: 10.84

GC content: 58.18

Gene sequence:

>1851_bases
ATGTCTCCTAACGATGCATTCATCTCCGCACCTGCCAAGATCGAAACCCCAGTTGGGCCTCGCAATGAAGGCCAGCCAGC
ATGGAATAAGCAGCGTGGCTCCTCAATGCCAGTTAACCGCTACATGCCTTTCGAGGTTGAGGTAGAAGATATTTCTCTGC
CGGACCGCACTTGGCCAGATAAAAAAATCACCGTTGCACCTCAGTGGTGTGCTGTTGACCTGCGTGACGGCAACCAGGCT
CTGATTGATCCGATGTCTCCTGAGCGTAAGCGCCGCATGTTTGAGCTGCTGGTTCAGATGGGCTTCAAGGAAATCGAAGT
CGGTTTCCCTTCAGCTTCCCAGACTGATTTTGATTTCGTTCGTGAGATCATCGAAAAGGACATGATCCCTGACGATGTCA
CCATTCAGGTTCTGGTTCAGGCTCGTGAGCACCTGATTCGCCGCACTTTTGAGGCATGTGAAGGCGCCAAGAACGTTATT
GTGCACTTCTACAACTCCACGTCCATCCTGCAGCGCAACGTTGTGTTCCGCATGGACAAGGACCAGGTAAAGAAGCTGGC
TACCGATGCCGCTGAACTGATCAAGACCATCGCGCAGGACTACCCAGACACCAACTGGCGCTGGCAGTACTCCCCTGAGT
CCTTCACCGGCACTGAGGTTGAGTACGCCAAGGAAGTTGTGGACGCCGTTGTTGAGGTCATGGATCCAACTCCTGAGAAC
CCAATGATCATCAACCTGCCTTCCACCGTTGAGATGATCACCCCTAACGTTTACGCAGACTCCATTGAATGGATGCACCG
CAATGTAAACCGTCGTGATTCCATTATCCTGTCCCTGCACCCGCACAATGACCGTGGCACCGGCGTTGGCGCAGCTGAAC
TGGGCTACATGGCTGGCGCTGACCGCATCGAAGGCTGCCTGTTCGGCAACGGCGAGCGCACCGGCAACGTCTGCCTGGTC
ACCCTGGCACTGAACATGCTGACCCAGGGCGTTGACCCTCAGCTGGACTTCACCGATATACGCCAGATCCGCAGCACCGT
TGAATACTGCAACCAGCTGCGCGTTCCTGAGCGCCACCCTTACGCCGGCGACCTGGTCTTCACCGCGTTCTCCGGCTCCC
ACCAGGATGCTGTTAACAAGGGTCTGGATGCGATGGCTGCCAAGGTCAAGCCAGGCGCGAACTCCACGGATGTGTCGTGG
GAGGAGCTGCGCGACACCGAATGGGAGGTTCCTTACCTGCCTATCGATCCAAAGGATGTCGGCCGCGACTACGAGGCCGT
TATTCGCGTGAACTCCCAGTCCGGCAAGGGCGGCGTTGCCTACATCATGAAGACCGATCATGGCCTGCAGATCCCTCGCT
CCATGCAGGTTGAGTTCTCCGCCGTTGTCCAGAACGTCACCGACGCTGAGGGCGGCGAGGTCAACTCCAAGGCAATGTGG
GATATCTTCGCCACCGAGTACCTGGAGCGCACCGCACCAGTTGAGCAGATCGCGCTGCGCGTCGAGAACGCTCAGACCGA
AAACGAGGATGCATCCATCACCGCCGAGCTCATCCACAACGGCAAGGACGTCACCGTCGATGGCCACGGCAACGGCCCAC
TGGCCGCTTACGCCAACGCGCTGGAGAAGCTGGGCATCGACGTTGAGATCCAGGAATACAACCAGCACGCCCGCACCTCG
GGCGACGATGCAGAAGCAGCCGCCTACGTCCTGGCTGAGGTCAACGGCCGCAAGGTCTGGGGCGTCGGCATCGCTGGCTC
CATCACCTACGCTTCGCTGAAGGCAGTGACCTCCGCCGTAAACCGCGCGCTGGACGTCAACCACGAGGCAGTCCTGGCTG
GCGGCGTTTAA

Upstream 100 bases:

>100_bases
CGGCGGGTCCCAGAGGTCTTAACACGACCGGCATCCCGTCGCGGAGTTTGGTGTTGCCGGTCGTGGACCCACCCAAAACT
TTTTAAGAAGGTTGAACACA

Downstream 100 bases:

>100_bases
GCTTTACGACGTCTCCCTCTAGGCCCTACAAGCCGGTGGCAAAATTTTCACGTTGTTGAAAATTCTTGCCACCGGCTTCG
TGTGTGATAGGCATTTAGAG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 616; Mature: 615

Protein sequence:

>616_residues
MSPNDAFISAPAKIETPVGPRNEGQPAWNKQRGSSMPVNRYMPFEVEVEDISLPDRTWPDKKITVAPQWCAVDLRDGNQA
LIDPMSPERKRRMFELLVQMGFKEIEVGFPSASQTDFDFVREIIEKDMIPDDVTIQVLVQAREHLIRRTFEACEGAKNVI
VHFYNSTSILQRNVVFRMDKDQVKKLATDAAELIKTIAQDYPDTNWRWQYSPESFTGTEVEYAKEVVDAVVEVMDPTPEN
PMIINLPSTVEMITPNVYADSIEWMHRNVNRRDSIILSLHPHNDRGTGVGAAELGYMAGADRIEGCLFGNGERTGNVCLV
TLALNMLTQGVDPQLDFTDIRQIRSTVEYCNQLRVPERHPYAGDLVFTAFSGSHQDAVNKGLDAMAAKVKPGANSTDVSW
EELRDTEWEVPYLPIDPKDVGRDYEAVIRVNSQSGKGGVAYIMKTDHGLQIPRSMQVEFSAVVQNVTDAEGGEVNSKAMW
DIFATEYLERTAPVEQIALRVENAQTENEDASITAELIHNGKDVTVDGHGNGPLAAYANALEKLGIDVEIQEYNQHARTS
GDDAEAAAYVLAEVNGRKVWGVGIAGSITYASLKAVTSAVNRALDVNHEAVLAGGV

Sequences:

>Translated_616_residues
MSPNDAFISAPAKIETPVGPRNEGQPAWNKQRGSSMPVNRYMPFEVEVEDISLPDRTWPDKKITVAPQWCAVDLRDGNQA
LIDPMSPERKRRMFELLVQMGFKEIEVGFPSASQTDFDFVREIIEKDMIPDDVTIQVLVQAREHLIRRTFEACEGAKNVI
VHFYNSTSILQRNVVFRMDKDQVKKLATDAAELIKTIAQDYPDTNWRWQYSPESFTGTEVEYAKEVVDAVVEVMDPTPEN
PMIINLPSTVEMITPNVYADSIEWMHRNVNRRDSIILSLHPHNDRGTGVGAAELGYMAGADRIEGCLFGNGERTGNVCLV
TLALNMLTQGVDPQLDFTDIRQIRSTVEYCNQLRVPERHPYAGDLVFTAFSGSHQDAVNKGLDAMAAKVKPGANSTDVSW
EELRDTEWEVPYLPIDPKDVGRDYEAVIRVNSQSGKGGVAYIMKTDHGLQIPRSMQVEFSAVVQNVTDAEGGEVNSKAMW
DIFATEYLERTAPVEQIALRVENAQTENEDASITAELIHNGKDVTVDGHGNGPLAAYANALEKLGIDVEIQEYNQHARTS
GDDAEAAAYVLAEVNGRKVWGVGIAGSITYASLKAVTSAVNRALDVNHEAVLAGGV
>Mature_615_residues
SPNDAFISAPAKIETPVGPRNEGQPAWNKQRGSSMPVNRYMPFEVEVEDISLPDRTWPDKKITVAPQWCAVDLRDGNQAL
IDPMSPERKRRMFELLVQMGFKEIEVGFPSASQTDFDFVREIIEKDMIPDDVTIQVLVQAREHLIRRTFEACEGAKNVIV
HFYNSTSILQRNVVFRMDKDQVKKLATDAAELIKTIAQDYPDTNWRWQYSPESFTGTEVEYAKEVVDAVVEVMDPTPENP
MIINLPSTVEMITPNVYADSIEWMHRNVNRRDSIILSLHPHNDRGTGVGAAELGYMAGADRIEGCLFGNGERTGNVCLVT
LALNMLTQGVDPQLDFTDIRQIRSTVEYCNQLRVPERHPYAGDLVFTAFSGSHQDAVNKGLDAMAAKVKPGANSTDVSWE
ELRDTEWEVPYLPIDPKDVGRDYEAVIRVNSQSGKGGVAYIMKTDHGLQIPRSMQVEFSAVVQNVTDAEGGEVNSKAMWD
IFATEYLERTAPVEQIALRVENAQTENEDASITAELIHNGKDVTVDGHGNGPLAAYANALEKLGIDVEIQEYNQHARTSG
DDAEAAAYVLAEVNGRKVWGVGIAGSITYASLKAVTSAVNRALDVNHEAVLAGGV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=546, Percent_Identity=25.8241758241758, Blast_Score=126, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324225, Length=578, Percent_Identity=46.1937716262976, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6324682, Length=558, Percent_Identity=46.7741935483871, Blast_Score=524, Evalue=1e-149,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_CORGB (A4QAP0)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001137189.1
- ProteinModelPortal:   A4QAP0
- SMR:   A4QAP0
- STRING:   A4QAP0
- GeneID:   4991673
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_0323
- eggNOG:   COG0119
- HOGENOM:   HBG289752
- OMA:   APRWCAV
- ProtClustDB:   PRK03739
- HAMAP:   MF_00572
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR10277:SF2
- SMART:   SM00917
- TIGRFAMs:   TIGR00970

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 68227; Mature: 68096

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPNDAFISAPAKIETPVGPRNEGQPAWNKQRGSSMPVNRYMPFEVEVEDISLPDRTWPD
CCCCCCEEECCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
KKITVAPQWCAVDLRDGNQALIDPMSPERKRRMFELLVQMGFKEIEVGFPSASQTDFDFV
CEEEECCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHH
REIIEKDMIPDDVTIQVLVQAREHLIRRTFEACEGAKNVIVHFYNSTSILQRNVVFRMDK
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHCCEEEEECH
DQVKKLATDAAELIKTIAQDYPDTNWRWQYSPESFTGTEVEYAKEVVDAVVEVMDPTPEN
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
PMIINLPSTVEMITPNVYADSIEWMHRNVNRRDSIILSLHPHNDRGTGVGAAELGYMAGA
CEEEECCCCEEEECCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCC
DRIEGCLFGNGERTGNVCLVTLALNMLTQGVDPQLDFTDIRQIRSTVEYCNQLRVPERHP
HHHCEEEECCCCCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
YAGDLVFTAFSGSHQDAVNKGLDAMAAKVKPGANSTDVSWEELRDTEWEVPYLPIDPKDV
CCCCEEEEEECCCCHHHHHHCHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHH
GRDYEAVIRVNSQSGKGGVAYIMKTDHGLQIPRSMQVEFSAVVQNVTDAEGGEVNSKAMW
CCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHH
DIFATEYLERTAPVEQIALRVENAQTENEDASITAELIHNGKDVTVDGHGNGPLAAYANA
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEHHHHHCCCEEEEECCCCCCHHHHHHH
LEKLGIDVEIQEYNQHARTSGDDAEAAAYVLAEVNGRKVWGVGIAGSITYASLKAVTSAV
HHHCCCCEEEHHHHHHHCCCCCCHHHHEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHH
NRALDVNHEAVLAGGV
HHHHCCCCCEEEECCC
>Mature Secondary Structure 
SPNDAFISAPAKIETPVGPRNEGQPAWNKQRGSSMPVNRYMPFEVEVEDISLPDRTWPD
CCCCCEEECCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
KKITVAPQWCAVDLRDGNQALIDPMSPERKRRMFELLVQMGFKEIEVGFPSASQTDFDFV
CEEEECCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHH
REIIEKDMIPDDVTIQVLVQAREHLIRRTFEACEGAKNVIVHFYNSTSILQRNVVFRMDK
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHCCEEEEECH
DQVKKLATDAAELIKTIAQDYPDTNWRWQYSPESFTGTEVEYAKEVVDAVVEVMDPTPEN
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
PMIINLPSTVEMITPNVYADSIEWMHRNVNRRDSIILSLHPHNDRGTGVGAAELGYMAGA
CEEEECCCCEEEECCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCC
DRIEGCLFGNGERTGNVCLVTLALNMLTQGVDPQLDFTDIRQIRSTVEYCNQLRVPERHP
HHHCEEEECCCCCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
YAGDLVFTAFSGSHQDAVNKGLDAMAAKVKPGANSTDVSWEELRDTEWEVPYLPIDPKDV
CCCCEEEEEECCCCHHHHHHCHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHH
GRDYEAVIRVNSQSGKGGVAYIMKTDHGLQIPRSMQVEFSAVVQNVTDAEGGEVNSKAMW
CCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHH
DIFATEYLERTAPVEQIALRVENAQTENEDASITAELIHNGKDVTVDGHGNGPLAAYANA
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEHHHHHCCCEEEEECCCCCCHHHHHHH
LEKLGIDVEIQEYNQHARTSGDDAEAAAYVLAEVNGRKVWGVGIAGSITYASLKAVTSAV
HHHCCCCEEEHHHHHHHCCCCCCHHHHEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHH
NRALDVNHEAVLAGGV
HHHHCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA