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The map label for this gene is ccpA [H]

Identifier: 145294304

GI number: 145294304

Start: 280092

End: 281084

Strand: Reverse

Name: ccpA [H]

Synonym: cgR_0260

Alternate gene names: 145294304

Gene position: 281084-280092 (Counterclockwise)

Preceding gene: 145294306

Following gene: 145294303

Centisome position: 8.48

GC content: 53.98

Gene sequence:

>993_bases
GTGAATGTGAAGTTAACTCATGTCGCCCGCGAAGCTGGAGTAGGTTACGGTACTGCTTCTCGCGCCATTTCCGGACGAGG
TTCCGTTGATGCAGCAACCCGTGACAAAGTGCTCGCCGCCGCCGAGAAACTTGGGAACCGAACCAACGCTATGGCACGTG
CTCTTCGGGAAAATAAGACCCGTACCGTTGGCTTGATCGTCCCCGACATTATCAATGAGTTCTACACCGAATCCGCCGCA
GTTCTTCAAAACGAACTAGACAAGTCTGGATACCAGCTGGTTGTTTCTACCATCGGAAATGACGCAGAAAAGGAACGTCG
AGCTATCGAATCCATGCTCAACCGCCAGGTAGATGCAGTGGTGCACGTTCCAGTTAATCCCCAAGCGAAGTTTCCAAAGG
GCTTCAAAGTGGTCGAGCTTAATCGTCGTAGCGACCTCAACCGACCCACTGTGACCAGCGATGATGCCACTGGTTTGAAG
GAACTTGCTCTTCATATTTTGGATCAGGGATACCGAGATATAGGTATCATTGTCGGTCCTGCTGAGCTCAGCACCGCCCG
AGACCGCAAAGCCGGATTCATCAACGCCCTCGAAACTGAAGCCACACAACGCGGAATCCGCGAAGAACTACGATTCCGGG
TAGTTCACTCCCGCTACTCCCCCACCGGCGGTTATGAAGCATTCGCAGAATTCCGCGACGATCTCCCTCAAATCGTGGTG
CCCCTGAGCACGCAATTAACTCTAGGAGTTCTCAAAGCAACCCAAGAAAACGGCATAAAAATACCGGATGACCTGGCACT
TGCTTGTTACGGCGTCGCCGAATGGCTCGCAGTGTGGGGCCCTGACATCACCGTTTTCGCACCAGACCTCCCAGCCATGG
GCGCCGCAGCTGCCACGCAGGTTTTAACGCTTCTCGACGCCGCCCCACTCCCCGAAAACCACTTAAGCATTCCGGGGCAG
CTCATTGTCCGTGGGACAACTCCAAAGGTTTAA

Upstream 100 bases:

>100_bases
ACCCATCGTGTTTAAATCTAGAAGTTTAAAGGGTGAAAACAGTCCATTACTTAAGCACCAATCTGCCATAATTTTTACCC
CAACGCATAGGCTTAACGGT

Downstream 100 bases:

>100_bases
AGGTAGAGGCGCACAACAATGAAAATTGAAATTTTCAGCCCAGAGGCCCTACAATCTGCTGAGTTCGCTCACCTTTTGTG
GCTTGCTTCCGGCTATTCTG

Product: hypothetical protein

Products: NA

Alternate protein names: Glucose-resistance amylase regulator [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MNVKLTHVAREAGVGYGTASRAISGRGSVDAATRDKVLAAAEKLGNRTNAMARALRENKTRTVGLIVPDIINEFYTESAA
VLQNELDKSGYQLVVSTIGNDAEKERRAIESMLNRQVDAVVHVPVNPQAKFPKGFKVVELNRRSDLNRPTVTSDDATGLK
ELALHILDQGYRDIGIIVGPAELSTARDRKAGFINALETEATQRGIREELRFRVVHSRYSPTGGYEAFAEFRDDLPQIVV
PLSTQLTLGVLKATQENGIKIPDDLALACYGVAEWLAVWGPDITVFAPDLPAMGAAAATQVLTLLDAAPLPENHLSIPGQ
LIVRGTTPKV

Sequences:

>Translated_330_residues
MNVKLTHVAREAGVGYGTASRAISGRGSVDAATRDKVLAAAEKLGNRTNAMARALRENKTRTVGLIVPDIINEFYTESAA
VLQNELDKSGYQLVVSTIGNDAEKERRAIESMLNRQVDAVVHVPVNPQAKFPKGFKVVELNRRSDLNRPTVTSDDATGLK
ELALHILDQGYRDIGIIVGPAELSTARDRKAGFINALETEATQRGIREELRFRVVHSRYSPTGGYEAFAEFRDDLPQIVV
PLSTQLTLGVLKATQENGIKIPDDLALACYGVAEWLAVWGPDITVFAPDLPAMGAAAATQVLTLLDAAPLPENHLSIPGQ
LIVRGTTPKV
>Mature_330_residues
MNVKLTHVAREAGVGYGTASRAISGRGSVDAATRDKVLAAAEKLGNRTNAMARALRENKTRTVGLIVPDIINEFYTESAA
VLQNELDKSGYQLVVSTIGNDAEKERRAIESMLNRQVDAVVHVPVNPQAKFPKGFKVVELNRRSDLNRPTVTSDDATGLK
ELALHILDQGYRDIGIIVGPAELSTARDRKAGFINALETEATQRGIREELRFRVVHSRYSPTGGYEAFAEFRDDLPQIVV
PLSTQLTLGVLKATQENGIKIPDDLALACYGVAEWLAVWGPDITVFAPDLPAMGAAAATQVLTLLDAAPLPENHLSIPGQ
LIVRGTTPKV

Specific function: Transcriptional regulator involved in both the repression of carbohydrate utilization genes such as the alpha- amylase (AmyE) and the acetyl-coenzyme A synthetase (AcsA); and in the positive regulation of genes involved in excretion of excess carbon such

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=25.4491017964072, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1786268, Length=295, Percent_Identity=31.864406779661, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789068, Length=298, Percent_Identity=27.1812080536913, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789202, Length=313, Percent_Identity=29.073482428115, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787948, Length=309, Percent_Identity=24.5954692556634, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790194, Length=328, Percent_Identity=24.0853658536585, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786540, Length=338, Percent_Identity=23.6686390532544, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI48994940, Length=306, Percent_Identity=24.8366013071895, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788474, Length=315, Percent_Identity=28.8888888888889, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787906, Length=203, Percent_Identity=30.0492610837438, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787580, Length=332, Percent_Identity=21.0843373493976, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006377
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35682; Mature: 35682

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVKLTHVAREAGVGYGTASRAISGRGSVDAATRDKVLAAAEKLGNRTNAMARALRENKT
CCEEHHHHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
RTVGLIVPDIINEFYTESAAVLQNELDKSGYQLVVSTIGNDAEKERRAIESMLNRQVDAV
CEEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCHHHHHHHHHHHHCCCCCEE
VHVPVNPQAKFPKGFKVVELNRRSDLNRPTVTSDDATGLKELALHILDQGYRDIGIIVGP
EECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECC
AELSTARDRKAGFINALETEATQRGIREELRFRVVHSRYSPTGGYEAFAEFRDDLPQIVV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEE
PLSTQLTLGVLKATQENGIKIPDDLALACYGVAEWLAVWGPDITVFAPDLPAMGAAAATQ
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHH
VLTLLDAAPLPENHLSIPGQLIVRGTTPKV
HHHHHHCCCCCCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MNVKLTHVAREAGVGYGTASRAISGRGSVDAATRDKVLAAAEKLGNRTNAMARALRENKT
CCEEHHHHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
RTVGLIVPDIINEFYTESAAVLQNELDKSGYQLVVSTIGNDAEKERRAIESMLNRQVDAV
CEEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCHHHHHHHHHHHHCCCCCEE
VHVPVNPQAKFPKGFKVVELNRRSDLNRPTVTSDDATGLKELALHILDQGYRDIGIIVGP
EECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECC
AELSTARDRKAGFINALETEATQRGIREELRFRVVHSRYSPTGGYEAFAEFRDDLPQIVV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEE
PLSTQLTLGVLKATQENGIKIPDDLALACYGVAEWLAVWGPDITVFAPDLPAMGAAAATQ
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHH
VLTLLDAAPLPENHLSIPGQLIVRGTTPKV
HHHHHHCCCCCCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1904524; 9387221; 9384377 [H]