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The map label for this gene is wcaJ [H]

Identifier: 145294077

GI number: 145294077

Start: 40149

End: 41435

Strand: Reverse

Name: wcaJ [H]

Synonym: cgR_0035

Alternate gene names: 145294077

Gene position: 41435-40149 (Counterclockwise)

Preceding gene: 145294081

Following gene: 145294076

Centisome position: 1.25

GC content: 49.34

Gene sequence:

>1287_bases
TTGGATACCGTAGCTATTTTTCTGTCTGCCTGGATTGTCGATATCCCATCGCCGTGGATCACAGCATTGGTGATGGTCAT
CATTTTGCAGGTTCTTCCAGATTTCTACCGGCATCGGCTTAATCTGTCTCTACTCAACGCGTTACCCACAGTCGTGCCCC
GAGCCTTAATTGTCGGGTTCTTCGCATACATTGTGGTCAATCCGCACGTCGATGCGAGGGATTCGTTTGATTTCATTGTG
GTCAGCACAATTGGCCTGATAATTTCTTGGGCACTGAGCTTTGCAGCTATTCGTTCCTGGCGGCGCCACGCACCTATTTC
TTTGCATCGCACGCTGGTGTTGGGTTCCGCCGACGATTCCATAGAGATCGTGACAGCGTTGAAGGAAAACCCGCAGTTTG
GGTTGTCACCTGTTGCTGTTATTGATTTGGAGTCGCAAGCAGCATCAGACGAGTTTCCAGAGGTAGAAACAGAATATTTC
TCGGATAACCTCACGGAATTATTGCAACGTCATGAGGCTGATGTTGTAATCGTGGCACCGAATCAGCATTCTGAAGAGTT
TCTTCTCAGTGCGTTCCGCGCGTCCCTTCGTGATCCAACTACCTTTTATGTCGTGCCCGCGCTTCCTCTTCAGGGGCAAA
GACATGACACTGAAGAATTAGGGCTCATCTCGCTTCGGCCTGTGAAGCGTTCGGCTTATCGATCGTTCTGGTGGCTGTTA
AAACGGCCGATTGATGTTACGTTGTCGCTGATAGCGATGCTTTTGTTGTCGCCTTTGATGGCAGTGCTCGCGATACTGGT
GAAATTGGATTCTCCACGTAATCCGTTGTTGTTCCGGCAGACTCGCATCGGCTTGGATGGAAAACCTTTTGAGCTGCTAA
AGTTTCGCACTTTAACGCCAGCAACGGTGAATGAGTCTGATGTGACATGGAATGTATCGCACGATGATCGGTTAACGCCG
TTGAATCGCATCATGCGGAAGTTCTCTCTTGATGAGCTTCCGCAGATTTTGAATGTTTTGCGCGGGGATATGACCTTGGT
GGGTCCTCGTCCAGAGCGCCCTCACTTTGTTGAGAAATTCGGTGCTGATTATCCGATTTATCATGATCGTCACCGTGTTC
CAGTTGGTTTGACGGGTTGGGCTGCCATCAATGGTCTGAGGGGCGATACCAGCATCAAAACTCGTGCTGAGTATGACAAT
TGGTACATCGAGCATTGGTCTATTTGGTTGGATGTGAAGATTTTGCTTCTGACTGTGCGTGCTGTTTTGAAGGGCACGGG
AGGATGA

Upstream 100 bases:

>100_bases
TGCTCCAAATATCCGCCCTCTACACCGGCAGGCCATACCCCGCACCGCTAGGCACGTAGCAGCGAAGTACACCCGCTTTT
CTAAATTTATACATCGAGTG

Downstream 100 bases:

>100_bases
CATCAATCGTCTTTGGTGCTCTGGCGCTGCGCCCTCATGGCAGTGGTGTGCAGACCTATCAGCGTGAGCTCATCCATGCG
CTCACCAAGGTTGAGGGGCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MDTVAIFLSAWIVDIPSPWITALVMVIILQVLPDFYRHRLNLSLLNALPTVVPRALIVGFFAYIVVNPHVDARDSFDFIV
VSTIGLIISWALSFAAIRSWRRHAPISLHRTLVLGSADDSIEIVTALKENPQFGLSPVAVIDLESQAASDEFPEVETEYF
SDNLTELLQRHEADVVIVAPNQHSEEFLLSAFRASLRDPTTFYVVPALPLQGQRHDTEELGLISLRPVKRSAYRSFWWLL
KRPIDVTLSLIAMLLLSPLMAVLAILVKLDSPRNPLLFRQTRIGLDGKPFELLKFRTLTPATVNESDVTWNVSHDDRLTP
LNRIMRKFSLDELPQILNVLRGDMTLVGPRPERPHFVEKFGADYPIYHDRHRVPVGLTGWAAINGLRGDTSIKTRAEYDN
WYIEHWSIWLDVKILLLTVRAVLKGTGG

Sequences:

>Translated_428_residues
MDTVAIFLSAWIVDIPSPWITALVMVIILQVLPDFYRHRLNLSLLNALPTVVPRALIVGFFAYIVVNPHVDARDSFDFIV
VSTIGLIISWALSFAAIRSWRRHAPISLHRTLVLGSADDSIEIVTALKENPQFGLSPVAVIDLESQAASDEFPEVETEYF
SDNLTELLQRHEADVVIVAPNQHSEEFLLSAFRASLRDPTTFYVVPALPLQGQRHDTEELGLISLRPVKRSAYRSFWWLL
KRPIDVTLSLIAMLLLSPLMAVLAILVKLDSPRNPLLFRQTRIGLDGKPFELLKFRTLTPATVNESDVTWNVSHDDRLTP
LNRIMRKFSLDELPQILNVLRGDMTLVGPRPERPHFVEKFGADYPIYHDRHRVPVGLTGWAAINGLRGDTSIKTRAEYDN
WYIEHWSIWLDVKILLLTVRAVLKGTGG
>Mature_428_residues
MDTVAIFLSAWIVDIPSPWITALVMVIILQVLPDFYRHRLNLSLLNALPTVVPRALIVGFFAYIVVNPHVDARDSFDFIV
VSTIGLIISWALSFAAIRSWRRHAPISLHRTLVLGSADDSIEIVTALKENPQFGLSPVAVIDLESQAASDEFPEVETEYF
SDNLTELLQRHEADVVIVAPNQHSEEFLLSAFRASLRDPTTFYVVPALPLQGQRHDTEELGLISLRPVKRSAYRSFWWLL
KRPIDVTLSLIAMLLLSPLMAVLAILVKLDSPRNPLLFRQTRIGLDGKPFELLKFRTLTPATVNESDVTWNVSHDDRLTP
LNRIMRKFSLDELPQILNVLRGDMTLVGPRPERPHFVEKFGADYPIYHDRHRVPVGLTGWAAINGLRGDTSIKTRAEYDN
WYIEHWSIWLDVKILLLTVRAVLKGTGG

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=424, Percent_Identity=30.4245283018868, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 48456; Mature: 48456

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTVAIFLSAWIVDIPSPWITALVMVIILQVLPDFYRHRLNLSLLNALPTVVPRALIVGF
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
FAYIVVNPHVDARDSFDFIVVSTIGLIISWALSFAAIRSWRRHAPISLHRTLVLGSADDS
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCC
IEIVTALKENPQFGLSPVAVIDLESQAASDEFPEVETEYFSDNLTELLQRHEADVVIVAP
EEEEEEHHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECC
NQHSEEFLLSAFRASLRDPTTFYVVPALPLQGQRHDTEELGLISLRPVKRSAYRSFWWLL
CCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHCCEEEECHHHHHHHHHHHHHH
KRPIDVTLSLIAMLLLSPLMAVLAILVKLDSPRNPLLFRQTRIGLDGKPFELLKFRTLTP
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHEEEEECCC
ATVNESDVTWNVSHDDRLTPLNRIMRKFSLDELPQILNVLRGDMTLVGPRPERPHFVEKF
CCCCCCCCEEECCCCCCCCHHHHHHHHHCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHH
GADYPIYHDRHRVPVGLTGWAAINGLRGDTSIKTRAEYDNWYIEHWSIWLDVKILLLTVR
CCCCCCCCCCCCCCCCEEHHHHHCCCCCCCCCEEECCCCCEEEEEEEHHHHHHHHHHHHH
AVLKGTGG
HHHHCCCC
>Mature Secondary Structure
MDTVAIFLSAWIVDIPSPWITALVMVIILQVLPDFYRHRLNLSLLNALPTVVPRALIVGF
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
FAYIVVNPHVDARDSFDFIVVSTIGLIISWALSFAAIRSWRRHAPISLHRTLVLGSADDS
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCC
IEIVTALKENPQFGLSPVAVIDLESQAASDEFPEVETEYFSDNLTELLQRHEADVVIVAP
EEEEEEHHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECC
NQHSEEFLLSAFRASLRDPTTFYVVPALPLQGQRHDTEELGLISLRPVKRSAYRSFWWLL
CCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHCCEEEECHHHHHHHHHHHHHH
KRPIDVTLSLIAMLLLSPLMAVLAILVKLDSPRNPLLFRQTRIGLDGKPFELLKFRTLTP
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHEEEEECCC
ATVNESDVTWNVSHDDRLTPLNRIMRKFSLDELPQILNVLRGDMTLVGPRPERPHFVEKF
CCCCCCCCEEECCCCCCCCHHHHHHHHHCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHH
GADYPIYHDRHRVPVGLTGWAAINGLRGDTSIKTRAEYDNWYIEHWSIWLDVKILLLTVR
CCCCCCCCCCCCCCCCEEHHHHHCCCCCCCCCEEECCCCCEEEEEEEHHHHHHHHHHHHH
AVLKGTGG
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]