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The map label for this gene is recF

Identifier: 145294046

GI number: 145294046

Start: 3585

End: 4769

Strand: Direct

Name: recF

Synonym: cgR_0004

Alternate gene names: 145294046

Gene position: 3585-4769 (Clockwise)

Preceding gene: 145294045

Following gene: 145294047

Centisome position: 0.11

GC content: 55.44

Gene sequence:

>1185_bases
ATGCACATCCGTTCTTTGGAATTACGTGATTACCGTTCCTGGCCTGAACTCAAAGTGGATTTGGAACCTGGAATTACAGT
TTTTATCGGCCGCAACGGTTTTGGTAAAACCAACATCGTCGAGGCCATCGGCTATCTTGCGCATTTGTCATCGCATCGGG
TGTCCTCTGATGCGCCATTGGTGCGGGCGCACGCTGAAAACGCCCGAGTTTCGGCGGTTGCTGTTAATCAAGGCCGAGAA
TTGGCAGCTCACTTGCTGATCAAACCCCATGCTGCGAATCAGGCAAGTTTGAATCGCACAAAAGTCAGGACTCCCCGGGA
GCTGCTTGGTGTGGTGAAGACTGTGCTGTTTGCGCCGGAAGATTTGGCATTAGTCAAAGGCGAGCCAGCAGAACGTCGCC
GCTATTTAGATGACATTATTGCCACTCGCCAGCCTCGGATGGCGGGAGTCAAGGCCGACTATGACAAGGTGCTGAAACAA
AGGAACGCCCTGCTCAAGACCGCCACCATTGCGCTTCGGCGAGGTTACGGCACCGAGGAAGGCGCAGCTGCGCTGAGTAC
TTTGGATACATGGGATGGCCAGTTGGCTCGCCTGGGTGCTGAAGTGATGGCAGCCAGATTTGCCCTGCTCAATGAGTTGG
GGCCGAAAATCTATGAGGCTTACACCACGATCGCCCCGGAATCCCGGCCAGCTGCGGTGAATTACAAAACCACCATCGAT
CAAGGCCTGTCGCAGTTTTCCGAATTCGATGCCGGCATCATCGAAGCCACGCTGCTGACAGAATTGGCAGCGAAACGTCA
ACGAGAAATCGAACGCGGCTCAAGCCTGGTCGGCCCCCACCGCGATGATGTCGATTTAATGCTCGGCGATCAGCCCGCCA
AAGGCTTTGCCAGCCACGGCGAGACCTGGTCTTTCGCGCTTTCACTGCGAATTGCAGAATTTAACCTGCTGAAATCCGAT
GACACCGACCCGATCCTCATCTTGGATGATGTGTTTTCCGAGCTCGACGCCGGCCGTCGCCAAAAACTCGTGGGCATAGC
GCAAGAGGTGGAACAGGTGCTCATCACCGCTGCGGTCCACGACGATCTGCCGGAGAATCTCAAGAAAGTGCTCACTGCGC
AGCACACCGTCACCGTCCAAGACACCGACACCGGGCGGATTTCACTCCTGGATGTGCAACCATGA

Upstream 100 bases:

>100_bases
GTTTCACCCTTTTCGCGCCTGACTTTGTACACTTTTCAACCGACAAAGTCAGGCGCGTTATCCAATCCACTTCCCACCGA
CTAAAAGGAGGTCAGACCCG

Downstream 100 bases:

>100_bases
CAGATCCAATTGAGCAGGCATTTGAACGCATCCGCGCCGAAGCCATGCGCAGAAATGGATCCGTTCCCGACCTCAATAAA
AACGATGCTTTTCGACGCCC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MHIRSLELRDYRSWPELKVDLEPGITVFIGRNGFGKTNIVEAIGYLAHLSSHRVSSDAPLVRAHAENARVSAVAVNQGRE
LAAHLLIKPHAANQASLNRTKVRTPRELLGVVKTVLFAPEDLALVKGEPAERRRYLDDIIATRQPRMAGVKADYDKVLKQ
RNALLKTATIALRRGYGTEEGAAALSTLDTWDGQLARLGAEVMAARFALLNELGPKIYEAYTTIAPESRPAAVNYKTTID
QGLSQFSEFDAGIIEATLLTELAAKRQREIERGSSLVGPHRDDVDLMLGDQPAKGFASHGETWSFALSLRIAEFNLLKSD
DTDPILILDDVFSELDAGRRQKLVGIAQEVEQVLITAAVHDDLPENLKKVLTAQHTVTVQDTDTGRISLLDVQP

Sequences:

>Translated_394_residues
MHIRSLELRDYRSWPELKVDLEPGITVFIGRNGFGKTNIVEAIGYLAHLSSHRVSSDAPLVRAHAENARVSAVAVNQGRE
LAAHLLIKPHAANQASLNRTKVRTPRELLGVVKTVLFAPEDLALVKGEPAERRRYLDDIIATRQPRMAGVKADYDKVLKQ
RNALLKTATIALRRGYGTEEGAAALSTLDTWDGQLARLGAEVMAARFALLNELGPKIYEAYTTIAPESRPAAVNYKTTID
QGLSQFSEFDAGIIEATLLTELAAKRQREIERGSSLVGPHRDDVDLMLGDQPAKGFASHGETWSFALSLRIAEFNLLKSD
DTDPILILDDVFSELDAGRRQKLVGIAQEVEQVLITAAVHDDLPENLKKVLTAQHTVTVQDTDTGRISLLDVQP
>Mature_394_residues
MHIRSLELRDYRSWPELKVDLEPGITVFIGRNGFGKTNIVEAIGYLAHLSSHRVSSDAPLVRAHAENARVSAVAVNQGRE
LAAHLLIKPHAANQASLNRTKVRTPRELLGVVKTVLFAPEDLALVKGEPAERRRYLDDIIATRQPRMAGVKADYDKVLKQ
RNALLKTATIALRRGYGTEEGAAALSTLDTWDGQLARLGAEVMAARFALLNELGPKIYEAYTTIAPESRPAAVNYKTTID
QGLSQFSEFDAGIIEATLLTELAAKRQREIERGSSLVGPHRDDVDLMLGDQPAKGFASHGETWSFALSLRIAEFNLLKSD
DTDPILILDDVFSELDAGRRQKLVGIAQEVEQVLITAAVHDDLPENLKKVLTAQHTVTVQDTDTGRISLLDVQP

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=393, Percent_Identity=25.1908396946565, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_CORGB (A4Q9S2)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001136867.1
- ProteinModelPortal:   A4Q9S2
- SMR:   A4Q9S2
- STRING:   A4Q9S2
- GeneID:   4993327
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_0004
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 43301; Mature: 43301

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIRSLELRDYRSWPELKVDLEPGITVFIGRNGFGKTNIVEAIGYLAHLSSHRVSSDAPL
CCCCCCCHHHCCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCE
VRAHAENARVSAVAVNQGRELAAHLLIKPHAANQASLNRTKVRTPRELLGVVKTVLFAPE
EEECCCCCEEEEEEECCCHHHHHHHEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCC
DLALVKGEPAERRRYLDDIIATRQPRMAGVKADYDKVLKQRNALLKTATIALRRGYGTEE
CCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
GAAALSTLDTWDGQLARLGAEVMAARFALLNELGPKIYEAYTTIAPESRPAAVNYKTTID
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEEHHHHH
QGLSQFSEFDAGIIEATLLTELAAKRQREIERGSSLVGPHRDDVDLMLGDQPAKGFASHG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHCCC
ETWSFALSLRIAEFNLLKSDDTDPILILDDVFSELDAGRRQKLVGIAQEVEQVLITAAVH
CCEEEEEEEEEEEEEEECCCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
DDLPENLKKVLTAQHTVTVQDTDTGRISLLDVQP
HCCHHHHHHHHHHCEEEEEEECCCCEEEEEECCC
>Mature Secondary Structure
MHIRSLELRDYRSWPELKVDLEPGITVFIGRNGFGKTNIVEAIGYLAHLSSHRVSSDAPL
CCCCCCCHHHCCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCE
VRAHAENARVSAVAVNQGRELAAHLLIKPHAANQASLNRTKVRTPRELLGVVKTVLFAPE
EEECCCCCEEEEEEECCCHHHHHHHEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCC
DLALVKGEPAERRRYLDDIIATRQPRMAGVKADYDKVLKQRNALLKTATIALRRGYGTEE
CCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
GAAALSTLDTWDGQLARLGAEVMAARFALLNELGPKIYEAYTTIAPESRPAAVNYKTTID
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEEHHHHH
QGLSQFSEFDAGIIEATLLTELAAKRQREIERGSSLVGPHRDDVDLMLGDQPAKGFASHG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHCCC
ETWSFALSLRIAEFNLLKSDDTDPILILDDVFSELDAGRRQKLVGIAQEVEQVLITAAVH
CCEEEEEEEEEEEEEEECCCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
DDLPENLKKVLTAQHTVTVQDTDTGRISLLDVQP
HCCHHHHHHHHHHCEEEEEEECCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA