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The map label for this gene is ykuT [H]

Identifier: 138897059

GI number: 138897059

Start: 3538394

End: 3539305

Strand: Reverse

Name: ykuT [H]

Synonym: GTNG_3432

Alternate gene names: 138897059

Gene position: 3539305-3538394 (Counterclockwise)

Preceding gene: 138897061

Following gene: 138897058

Centisome position: 99.69

GC content: 47.48

Gene sequence:

>912_bases
GTGTCGTCAACGTTGAAGACAATTGAAAAAACGGTGAATCCGTTCTTTGAGTTTTTGACAGATGAGAACTTGTGGACTGA
GATCGGAAAAAAGACGCTTTATATTATTCTTATCCTACTCATATGCGCCGTAGCGGTGAAAGTATTGAAAATGGCCGTCC
ATAATGCATTCAAAATGCGCCAGAAAGCACCGCTCCGATTTTCGGAACGTCGGGAGATGACGCTAGCGCGACTGCTCGAC
AACGTCATTACATACGCATTATATTTTATTGCGTTATTGATGATTTTAGATACGTTGGGTGTTCCCGTTAAAGGGCTGTT
GGCCGGGGCTGGGGTCGTCGGCTTGGCCGTTGGCTTCGGGGCGCAGAGTTTAGTGAAAGACATCATTAGCGGATTTTTCA
TCATTTTTGAAGACCAGTTTGCTGTCGGCGATTACGTGCGCATCGACAATTTTGAAGGGTATGTGGAAGAAATTGGGTTG
CGGGTAACGAAAATTAAAAGTTGGACTGGAGAAGTGCACATTTTACCGAACGGCAGTATCACGCAAGTGACAAACTATTC
GCTTCATAATAGCTTGGCGGTTGTAGACGTCAGCATCGCTTACGAAGAGGATATTGAAGAGGCAGAACAAGCAATTCGCG
AGCTTCTGCCGCAGCTGCCGGCAAAATATGAAGATATGGTCGCTCCGCCGGAACTGCTCGGCGTGCAAAATGTGACCGGT
CCGGAAGTCGTGCTCCGCATTGTATGTGAAACAAAACCGATGTGCCATATCGGTATAGGAAGAGCCATTCGCAAAGAAGT
AAAGATGCTTTTAGACGAACGAGGGATCGAAGTTGCGTATCCACGACTCGTGCTGCATCGCCGGGATGAAGACGAACAGT
CTGCGGTGAAGCGGCAAAGGGAACAATCGTGA

Upstream 100 bases:

>100_bases
ATACATCTTGACGTTTGTTTTTCTTTTTCATCTGGTGCATGACTACAAATCAACGATCGATCTTGTTAAAATAAAAAGCG
CAGAATGAAATAAGGTAGAG

Downstream 100 bases:

>100_bases
TGATATGGGGGTGAGTGGATGGAAGGGAAAGAATTTGGTTTGTATGATATCGTTGAAATGAAAAAACCGCATCCGTGCGG
TACGAACCGTTGGAAGGTGA

Product: mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSSTLKTIEKTVNPFFEFLTDENLWTEIGKKTLYIILILLICAVAVKVLKMAVHNAFKMRQKAPLRFSERREMTLARLLD
NVITYALYFIALLMILDTLGVPVKGLLAGAGVVGLAVGFGAQSLVKDIISGFFIIFEDQFAVGDYVRIDNFEGYVEEIGL
RVTKIKSWTGEVHILPNGSITQVTNYSLHNSLAVVDVSIAYEEDIEEAEQAIRELLPQLPAKYEDMVAPPELLGVQNVTG
PEVVLRIVCETKPMCHIGIGRAIRKEVKMLLDERGIEVAYPRLVLHRRDEDEQSAVKRQREQS

Sequences:

>Translated_303_residues
MSSTLKTIEKTVNPFFEFLTDENLWTEIGKKTLYIILILLICAVAVKVLKMAVHNAFKMRQKAPLRFSERREMTLARLLD
NVITYALYFIALLMILDTLGVPVKGLLAGAGVVGLAVGFGAQSLVKDIISGFFIIFEDQFAVGDYVRIDNFEGYVEEIGL
RVTKIKSWTGEVHILPNGSITQVTNYSLHNSLAVVDVSIAYEEDIEEAEQAIRELLPQLPAKYEDMVAPPELLGVQNVTG
PEVVLRIVCETKPMCHIGIGRAIRKEVKMLLDERGIEVAYPRLVLHRRDEDEQSAVKRQREQS
>Mature_302_residues
SSTLKTIEKTVNPFFEFLTDENLWTEIGKKTLYIILILLICAVAVKVLKMAVHNAFKMRQKAPLRFSERREMTLARLLDN
VITYALYFIALLMILDTLGVPVKGLLAGAGVVGLAVGFGAQSLVKDIISGFFIIFEDQFAVGDYVRIDNFEGYVEEIGLR
VTKIKSWTGEVHILPNGSITQVTNYSLHNSLAVVDVSIAYEEDIEEAEQAIRELLPQLPAKYEDMVAPPELLGVQNVTGP
EVVLRIVCETKPMCHIGIGRAIRKEVKMLLDERGIEVAYPRLVLHRRDEDEQSAVKRQREQS

Specific function: May play a role in resistance to osmotic downshock [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI87081787, Length=257, Percent_Identity=31.9066147859922, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787591, Length=158, Percent_Identity=28.4810126582279, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786670, Length=225, Percent_Identity=25.3333333333333, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI2367355, Length=133, Percent_Identity=30.0751879699248, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789291, Length=240, Percent_Identity=22.5, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 34048; Mature: 33917

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTLKTIEKTVNPFFEFLTDENLWTEIGKKTLYIILILLICAVAVKVLKMAVHNAFKMR
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKAPLRFSERREMTLARLLDNVITYALYFIALLMILDTLGVPVKGLLAGAGVVGLAVGFG
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHCC
AQSLVKDIISGFFIIFEDQFAVGDYVRIDNFEGYVEEIGLRVTKIKSWTGEVHILPNGSI
HHHHHHHHHCCEEEEEECCCCCCCEEEECCCHHHHHHHCCHHEEEECCCCEEEEECCCCE
TQVTNYSLHNSLAVVDVSIAYEEDIEEAEQAIRELLPQLPAKYEDMVAPPELLGVQNVTG
EEEECCHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHCCCCCCC
PEVVLRIVCETKPMCHIGIGRAIRKEVKMLLDERGIEVAYPRLVLHRRDEDEQSAVKRQR
HHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCHHHHHHHHHH
EQS
CCC
>Mature Secondary Structure 
SSTLKTIEKTVNPFFEFLTDENLWTEIGKKTLYIILILLICAVAVKVLKMAVHNAFKMR
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKAPLRFSERREMTLARLLDNVITYALYFIALLMILDTLGVPVKGLLAGAGVVGLAVGFG
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHCC
AQSLVKDIISGFFIIFEDQFAVGDYVRIDNFEGYVEEIGLRVTKIKSWTGEVHILPNGSI
HHHHHHHHHCCEEEEEECCCCCCCEEEECCCHHHHHHHCCHHEEEECCCCEEEEECCCCE
TQVTNYSLHNSLAVVDVSIAYEEDIEEAEQAIRELLPQLPAKYEDMVAPPELLGVQNVTG
EEEECCHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHCCCCCCC
PEVVLRIVCETKPMCHIGIGRAIRKEVKMLLDERGIEVAYPRLVLHRRDEDEQSAVKRQR
HHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCHHHHHHHHHH
EQS
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]