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The map label for this gene is purA [H]

Identifier: 138897045

GI number: 138897045

Start: 3523379

End: 3524698

Strand: Reverse

Name: purA [H]

Synonym: GTNG_3416

Alternate gene names: 138897045

Gene position: 3524698-3523379 (Counterclockwise)

Preceding gene: 138897046

Following gene: 138897044

Centisome position: 99.28

GC content: 52.35

Gene sequence:

>1320_bases
ATGTTTAGACCGTGGAAACTGGAGGTGCTCGCCATGTCGTCAGTTGTTGTTGTCGGCACACAATGGGGCGATGAAGGGAA
AGGAAAAATTACTGACTTTTTATCGGAAAATGCGGAAGTGATTGCCCGATACCAAGGAGGAAACAACGCCGGACACACGA
TCGTGTTTAACGGGGAAAAGTACAAGCTGCACTTAATCCCGTCGGGCATTTTTTATAAAGATAAAATTTGTGTCATCGGC
AACGGAATGGTTGTCGACCCGAAAGCGCTTGTCGCTGAACTCTCATATTTGCATGAACACGGCATTTCGACGGATAATTT
ACGTATTAGCAACCGCGCTCATGTTATTTTGCCATACCATTTGAAACTGGATGGGCTCGAGGAAGAGCGGAAAGGTGCGA
ACAAAATCGGCACGACGAAAAAAGGGATTGGCCCGGCATACATGGACAAAGCGGCGCGCATCGGCATTCGTATCGTTGAT
TTGCTTGACCGCGATGTGTTCGCGGAAAAGTTGGCCCGCAATTTAGAAGAGAAAAACATGTTGTTTGAAAAAGTGTACGG
GGTCGAAGGCTTCCGGCACGAGGACATTTTCGAAGAATACTACGAGTACGGTCAACAAATCGCTAAGTACGTTTGCGACA
CATCAGTCGTGTTGAACAATGCGCTCGATGAAGGGCGTCGCGTCTTGTTTGAAGGGGCGCAAGGTGTCATGCTCGATATC
GACCAAGGGACATATCCGTTTGTTACGTCATCCAACCCGGTAGCTGGTGGGGTGACGATCGGCGCTGGTGTCGGCCCAAC
GAAAATTAAACATGTCGTTGGGGTGGCGAAAGCATACACGACGCGCGTCGGCGATGGGCCGTTCCCGACCGAGCTGCACG
ATGAAATTGGCGACCGCATCCGCGAAGTCGGCCGCGAGTATGGGACGACAACCGGCCGTCCGCGCCGCGTCGGCTGGTTT
GACAGCGTCGTCGTCCGCCACGCCCGTCGGGTGAGCGGTATCACCGACTTGTCGCTCAATTCGATTGACGTCTTAACCGG
TATTGAAACATTGAAAATTTGCGTGGCCTACCGTTATCGTGGTGAAGTGATTGAAGAATTTCCAGCCAGCTTAAAAGTAT
TGGCCGAATGTGAGCCGATTTATGAAGAGCTGCCAGGCTGGACGGAAGACATTACTGGTGTGAGAAGCTTGGACGAGCTG
CCGGCGAACGCTCGCCACTATGTCGAGCGCATTTCCCAGCTCACCGGCATCCCGCTCTCGATTTTCTCCGTCGGCCCGGA
TCGCTCGCAAACGAATGTCGTCCGCAGCGTCTACGCGTAA

Upstream 100 bases:

>100_bases
TCTTTTCATGTCTCTGGGTAATAAACGAATAAAATAGTTTATAATTGTTTTTAATGTTCGTGTTTCATTGACTTTATGCC
TGAAAACTGTTACACTTACA

Downstream 100 bases:

>100_bases
GCGAGTGGCGGTCGACAGGAAGCAGGCATAGATGCTTGTGCGTCTATGCCTTTTTCATTGTTTTCGCGCCGGAGATCACA
TTTTCGAAGCCACTTGCCTT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MFRPWKLEVLAMSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGNNAGHTIVFNGEKYKLHLIPSGIFYKDKICVIG
NGMVVDPKALVAELSYLHEHGISTDNLRISNRAHVILPYHLKLDGLEEERKGANKIGTTKKGIGPAYMDKAARIGIRIVD
LLDRDVFAEKLARNLEEKNMLFEKVYGVEGFRHEDIFEEYYEYGQQIAKYVCDTSVVLNNALDEGRRVLFEGAQGVMLDI
DQGTYPFVTSSNPVAGGVTIGAGVGPTKIKHVVGVAKAYTTRVGDGPFPTELHDEIGDRIREVGREYGTTTGRPRRVGWF
DSVVVRHARRVSGITDLSLNSIDVLTGIETLKICVAYRYRGEVIEEFPASLKVLAECEPIYEELPGWTEDITGVRSLDEL
PANARHYVERISQLTGIPLSIFSVGPDRSQTNVVRSVYA

Sequences:

>Translated_439_residues
MFRPWKLEVLAMSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGNNAGHTIVFNGEKYKLHLIPSGIFYKDKICVIG
NGMVVDPKALVAELSYLHEHGISTDNLRISNRAHVILPYHLKLDGLEEERKGANKIGTTKKGIGPAYMDKAARIGIRIVD
LLDRDVFAEKLARNLEEKNMLFEKVYGVEGFRHEDIFEEYYEYGQQIAKYVCDTSVVLNNALDEGRRVLFEGAQGVMLDI
DQGTYPFVTSSNPVAGGVTIGAGVGPTKIKHVVGVAKAYTTRVGDGPFPTELHDEIGDRIREVGREYGTTTGRPRRVGWF
DSVVVRHARRVSGITDLSLNSIDVLTGIETLKICVAYRYRGEVIEEFPASLKVLAECEPIYEELPGWTEDITGVRSLDEL
PANARHYVERISQLTGIPLSIFSVGPDRSQTNVVRSVYA
>Mature_439_residues
MFRPWKLEVLAMSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGNNAGHTIVFNGEKYKLHLIPSGIFYKDKICVIG
NGMVVDPKALVAELSYLHEHGISTDNLRISNRAHVILPYHLKLDGLEEERKGANKIGTTKKGIGPAYMDKAARIGIRIVD
LLDRDVFAEKLARNLEEKNMLFEKVYGVEGFRHEDIFEEYYEYGQQIAKYVCDTSVVLNNALDEGRRVLFEGAQGVMLDI
DQGTYPFVTSSNPVAGGVTIGAGVGPTKIKHVVGVAKAYTTRVGDGPFPTELHDEIGDRIREVGREYGTTTGRPRRVGWF
DSVVVRHARRVSGITDLSLNSIDVLTGIETLKICVAYRYRGEVIEEFPASLKVLAECEPIYEELPGWTEDITGVRSLDEL
PANARHYVERISQLTGIPLSIFSVGPDRSQTNVVRSVYA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=422, Percent_Identity=42.4170616113744, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI22748717, Length=422, Percent_Identity=42.654028436019, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI40316944, Length=392, Percent_Identity=41.3265306122449, Blast_Score=288, Evalue=9e-78,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=47.6635514018692, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25146553, Length=422, Percent_Identity=42.8909952606635, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI25146555, Length=422, Percent_Identity=42.8909952606635, Blast_Score=335, Evalue=4e-92,
Organism=Saccharomyces cerevisiae, GI6324109, Length=425, Percent_Identity=40.2352941176471, Blast_Score=331, Evalue=1e-91,
Organism=Drosophila melanogaster, GI21356033, Length=417, Percent_Identity=43.1654676258993, Blast_Score=338, Evalue=4e-93,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 48693; Mature: 48693

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRPWKLEVLAMSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGNNAGHTIVFNGEK
CCCCEEEEEEEECEEEEEEECCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEECCCE
YKLHLIPSGIFYKDKICVIGNGMVVDPKALVAELSYLHEHGISTDNLRISNRAHVILPYH
EEEEEECCCCEEECEEEEEECCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEE
LKLDGLEEERKGANKIGTTKKGIGPAYMDKAARIGIRIVDLLDRDVFAEKLARNLEEKNM
EEECCCCHHHCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LFEKVYGVEGFRHEDIFEEYYEYGQQIAKYVCDTSVVLNNALDEGRRVLFEGAQGVMLDI
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEE
DQGTYPFVTSSNPVAGGVTIGAGVGPTKIKHVVGVAKAYTTRVGDGPFPTELHDEIGDRI
CCCCCCEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
REVGREYGTTTGRPRRVGWFDSVVVRHARRVSGITDLSLNSIDVLTGIETLKICVAYRYR
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GEVIEEFPASLKVLAECEPIYEELPGWTEDITGVRSLDELPANARHYVERISQLTGIPLS
HHHHHHCCHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEE
IFSVGPDRSQTNVVRSVYA
EEECCCCCCHHHHHHHHCC
>Mature Secondary Structure
MFRPWKLEVLAMSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGNNAGHTIVFNGEK
CCCCEEEEEEEECEEEEEEECCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEECCCE
YKLHLIPSGIFYKDKICVIGNGMVVDPKALVAELSYLHEHGISTDNLRISNRAHVILPYH
EEEEEECCCCEEECEEEEEECCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEE
LKLDGLEEERKGANKIGTTKKGIGPAYMDKAARIGIRIVDLLDRDVFAEKLARNLEEKNM
EEECCCCHHHCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LFEKVYGVEGFRHEDIFEEYYEYGQQIAKYVCDTSVVLNNALDEGRRVLFEGAQGVMLDI
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEE
DQGTYPFVTSSNPVAGGVTIGAGVGPTKIKHVVGVAKAYTTRVGDGPFPTELHDEIGDRI
CCCCCCEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
REVGREYGTTTGRPRRVGWFDSVVVRHARRVSGITDLSLNSIDVLTGIETLKICVAYRYR
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GEVIEEFPASLKVLAECEPIYEELPGWTEDITGVRSLDELPANARHYVERISQLTGIPLS
HHHHHHCCHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEE
IFSVGPDRSQTNVVRSVYA
EEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA