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The map label for this gene is prfA [H]

Identifier: 138896954

GI number: 138896954

Start: 3428930

End: 3430006

Strand: Reverse

Name: prfA [H]

Synonym: GTNG_3323

Alternate gene names: 138896954

Gene position: 3430006-3428930 (Counterclockwise)

Preceding gene: 138896955

Following gene: 138896953

Centisome position: 96.61

GC content: 51.07

Gene sequence:

>1077_bases
GTGTTTGATCGGTTAGAAGCTGTTGAACAACGATATGAAAAATTAAACGAGCTTTTGATGGATCCAGCCGTTATCAACGA
CCCGAAAAAGTTGCGCGATTATTCGAAAGAGCAGGCTGATTTGACTGAAACGGTGCAAACGTACCGTGAATACAAGTCCG
TTCGCAGTCAGCTCGCGGAAGCGAAGGCTATGCTGGAAGAAAAACTTGAGCCAGAGCTGCGCGAGATGGTGAAAGAGGAA
ATTGATGAGCTCGAAGAACGGGAAGAAGCGCTCGTTGAGAAGTTGAAAGTGTTGCTTTTGCCGAAAGATCCGAATGATGA
GAAAAACGTCATTATGGAAATTCGTGCCGCCGCCGGTGGCGAGGAAGCCGCGCTGTTTGCCGGCGACTTGTACCGGATGT
ATACGCGCTATGCGGAGTCGCAAGGGTGGAAAACGGAAGTAATCGAAGCAAGCCCAACAGGCCTTGGCGGCTATAAAGAA
ATCATCTTTATGGTCAATGGGAAAGGGGCGTATTCGAAGCTGAAGTTTGAAAACGGCGCTCATCGCGTCCAACGCGTTCC
GGAAACGGAATCAGGCGGACGCATCCATACATCGACGGCAACGGTCGCCTGCTTGCCGGAAATGGAAGAAGTCGAAGTCG
AAATTCATGAAAAAGACATTCGCGTCGATACGTACGCCTCGAGCGGGCCAGGGGGACAAAGCGTGAACACGACGATGTCA
GCCGTACGCCTCACCCATATTCCGACCGGCATTGTCGTTACTTGCCAAGACGAAAAATCGCAAATTAAAAACAAAGAAAA
AGCGATGAAAGTGTTGCGCGCCCGCATTTACGACAAATACCAGCAAGAAGCGCGCGCCGAGTATGACCAAACGCGTAAGC
AAGCGGTCGGCACCGGTGATCGCTCAGAGCGCATCCGCACGTACAACTTCCCGCAAAACCGCGTCACTGACCACCGTATC
GGGTTGACGATTCAAAAGCTTGACCTCGTGTTAGACGGGCAGCTCGATGAAATTATCGAGGCGCTCATTTTAGACGACCA
GTCGAAAAAACTGGAGCAAGCGAACGATGCGTCGTAA

Upstream 100 bases:

>100_bases
AATTAAACGCTCCCGCTTACGAAAAGCGGGGGTTTTTGTTTGGTTTTGTTTCATGATATAATTTAAAATTAGACTGTGCT
GAAAAACAGAGGTGAATGCT

Downstream 100 bases:

>100_bases
CGTACATGAGGTCCTCACCTGGGCTTCTTCTTTTTTGCATGCTCATGGAAAGGAAGAGCGGGCGGCCGAGTGGCTATTGT
GTCACCATTTGGGCACTGAC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MFDRLEAVEQRYEKLNELLMDPAVINDPKKLRDYSKEQADLTETVQTYREYKSVRSQLAEAKAMLEEKLEPELREMVKEE
IDELEEREEALVEKLKVLLLPKDPNDEKNVIMEIRAAAGGEEAALFAGDLYRMYTRYAESQGWKTEVIEASPTGLGGYKE
IIFMVNGKGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVACLPEMEEVEVEIHEKDIRVDTYASSGPGGQSVNTTMS
AVRLTHIPTGIVVTCQDEKSQIKNKEKAMKVLRARIYDKYQQEARAEYDQTRKQAVGTGDRSERIRTYNFPQNRVTDHRI
GLTIQKLDLVLDGQLDEIIEALILDDQSKKLEQANDAS

Sequences:

>Translated_358_residues
MFDRLEAVEQRYEKLNELLMDPAVINDPKKLRDYSKEQADLTETVQTYREYKSVRSQLAEAKAMLEEKLEPELREMVKEE
IDELEEREEALVEKLKVLLLPKDPNDEKNVIMEIRAAAGGEEAALFAGDLYRMYTRYAESQGWKTEVIEASPTGLGGYKE
IIFMVNGKGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVACLPEMEEVEVEIHEKDIRVDTYASSGPGGQSVNTTMS
AVRLTHIPTGIVVTCQDEKSQIKNKEKAMKVLRARIYDKYQQEARAEYDQTRKQAVGTGDRSERIRTYNFPQNRVTDHRI
GLTIQKLDLVLDGQLDEIIEALILDDQSKKLEQANDAS
>Mature_358_residues
MFDRLEAVEQRYEKLNELLMDPAVINDPKKLRDYSKEQADLTETVQTYREYKSVRSQLAEAKAMLEEKLEPELREMVKEE
IDELEEREEALVEKLKVLLLPKDPNDEKNVIMEIRAAAGGEEAALFAGDLYRMYTRYAESQGWKTEVIEASPTGLGGYKE
IIFMVNGKGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVACLPEMEEVEVEIHEKDIRVDTYASSGPGGQSVNTTMS
AVRLTHIPTGIVVTCQDEKSQIKNKEKAMKVLRARIYDKYQQEARAEYDQTRKQAVGTGDRSERIRTYNFPQNRVTDHRI
GLTIQKLDLVLDGQLDEIIEALILDDQSKKLEQANDAS

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=288, Percent_Identity=45.4861111111111, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI34577120, Length=349, Percent_Identity=37.8223495702006, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI166795305, Length=193, Percent_Identity=43.5233160621762, Blast_Score=168, Evalue=6e-42,
Organism=Escherichia coli, GI1787462, Length=349, Percent_Identity=51.5759312320917, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=38.3480825958702, Blast_Score=221, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI17542784, Length=272, Percent_Identity=38.9705882352941, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=317, Percent_Identity=43.5331230283912, Blast_Score=235, Evalue=7e-63,
Organism=Drosophila melanogaster, GI19921226, Length=339, Percent_Identity=40.7079646017699, Blast_Score=244, Evalue=9e-65,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40737; Mature: 40737

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDRLEAVEQRYEKLNELLMDPAVINDPKKLRDYSKEQADLTETVQTYREYKSVRSQLAE
CCHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAMLEEKLEPELREMVKEEIDELEEREEALVEKLKVLLLPKDPNDEKNVIMEIRAAAGG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHEEHHHCCC
EEAALFAGDLYRMYTRYAESQGWKTEVIEASPTGLGGYKEIIFMVNGKGAYSKLKFENGA
CCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCEEEECCCH
HRVQRVPETESGGRIHTSTATVACLPEMEEVEVEIHEKDIRVDTYASSGPGGQSVNTTMS
HHHHCCCCCCCCCEEEECCEEEEECCCHHHHHEEEECCCEEEEECCCCCCCCCCHHHHHH
AVRLTHIPTGIVVTCQDEKSQIKNKEKAMKVLRARIYDKYQQEARAEYDQTRKQAVGTGD
HHHHEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSERIRTYNFPQNRVTDHRIGLTIQKLDLVLDGQLDEIIEALILDDQSKKLEQANDAS
CCCCCEECCCCCCCCCCHHHCEEEEHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCC
>Mature Secondary Structure
MFDRLEAVEQRYEKLNELLMDPAVINDPKKLRDYSKEQADLTETVQTYREYKSVRSQLAE
CCHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAMLEEKLEPELREMVKEEIDELEEREEALVEKLKVLLLPKDPNDEKNVIMEIRAAAGG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHEEHHHCCC
EEAALFAGDLYRMYTRYAESQGWKTEVIEASPTGLGGYKEIIFMVNGKGAYSKLKFENGA
CCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCEEEECCCH
HRVQRVPETESGGRIHTSTATVACLPEMEEVEVEIHEKDIRVDTYASSGPGGQSVNTTMS
HHHHCCCCCCCCCEEEECCEEEEECCCHHHHHEEEECCCEEEEECCCCCCCCCCHHHHHH
AVRLTHIPTGIVVTCQDEKSQIKNKEKAMKVLRARIYDKYQQEARAEYDQTRKQAVGTGD
HHHHEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSERIRTYNFPQNRVTDHRIGLTIQKLDLVLDGQLDEIIEALILDDQSKKLEQANDAS
CCCCCEECCCCCCCCCCHHHCEEEEHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA