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The map label for this gene is 138896797

Identifier: 138896797

GI number: 138896797

Start: 3260439

End: 3261923

Strand: Reverse

Name: 138896797

Synonym: GTNG_3160

Alternate gene names: NA

Gene position: 3261923-3260439 (Counterclockwise)

Preceding gene: 138896798

Following gene: 138896796

Centisome position: 91.88

GC content: 54.95

Gene sequence:

>1485_bases
ATGAGGATGGGGATCCGCACAGGTGCCCAGTACATCAGTGGATTGAAGTCCCGGAAGCCAGAAATCTGGTTGAGTGGCCG
TCGCGTGATCAACGTATGCGAAGAACCTGTGTTCAAACAACCAATCCGCGAAATCGCCAGACTCTATGATATGCAGCATG
ATCCCGAGTATCAGGACAAGATTACACACATTTGCACAGAAACCGGAGAGCGGGTGAGCAACGCGTTCCTGGTCCCTAAA
TCTCGGGAGGATCTGCTAGCCCGCCGTGCGTTGTTTGAAGTATGGGCCCGCGCCACGTTTGGTCTGATGGGGAGAACTCC
TGATTTTCTCAATGTCGTGCTCACCTCGCTGTACAGCAACGCTTCGTTTTTGGAAAAGTACAATCCGCAGTGGGCGGAAA
ACATCCGTGCGTACTACCGCTATGTGCGCGACAACGACCTGTTTTTGACGCACGCCATCATCAATCCGCAAAACGACCGG
AGCAAGCCGTCCCACGAACAGCAGGACACGTTTACGCACCTAGGAGTGGTAAGGGAGACGCCGGAGGGGTTGATTGTGAG
AGGTGCCAAGATGCTGGCCACACTGGCACCGATCACGGACGAGGTGATCATCTATACCTTCCCTGGATATAAGCCAGGTG
ATGAGCGGTACGCGGTCTCGTTTGCTATCCCGATCGACACGCCAGGGCTGCGCATTCTCTGCCGAGAACCAATGCAGGAT
GGCACACGCCCGCTGTTTGACCATCCGCTAGCATCCCGCTTCGAGGAAATGGATGCGCTCTTGGTGTTCAACGATGTGCT
GGTGCCATGGGACCGGGTGTTCATCTACAATAACGTGGAAGCGGCTAACCTGCTCTATCCGAAGACCGGTATCGCGCAGC
AACCTGCTCATCAAACAGGAGTGCGCGGTCTGATTAAGCTGCAGTTCGCCACCGAAGTAGCTATTCGTTTGGCCGATTCG
ATCGGGGTGGACGTCTATCTGAACGTGCAAAATGACCTAGGTGAGCTGCTCCAGTCGGTAGAAGCAATCCGCGCATTGCT
GCACCTAGCTGAACACGAACTGGAGGTGTTGCCCTCCGGCGAGGTGATGCCAGGATGGGTGCCGCTTGAGACGATTCGTG
GTCTCCTGCCCAAGCTGTATCCACGTGCTGTGGAAGTATTACAAATCATCGGTGCCGGTGGCCTGTTGATGTCCCCAACG
GGTGCCGACTTTGCTAATCCGGAACTAGCGGCAGACATGGAGAAATACTACGCTGGTCGCATTGGAGTGGGCGGAGAGGA
GAGGGTTCGGCTGTTCAAACTGGCTTGGGATTTATGTGGCGAAGCATTTGGCCAGAGACTCCTGCAGTATGAGCGTTTCT
ACACTGGCGACCCGATCCGCAAGCGGGCGATCTTCTATAACAACATCAAAAGGGAACGTACGCTCGTCATGGTGGATGAG
GCTCTGCGGATGCCGAATCAGCAGGAGAAAGTTGTGAATGCCTGA

Upstream 100 bases:

>100_bases
AAACAAAGGAAAAACGTTAATTAGAATACAATGTTCAGACTAGTCGGATTATTATAATGGTTCACAAAGGATGACTCTAA
TAATTTGATACAAGGAGGTT

Downstream 100 bases:

>100_bases
TGAAGGTGCATGAGGCAAATCAGGGGTTGACGCTACCCGGCTCGGTGGTGGCGATAGGGGCTTTCGACGGAGTGCACCAG
GGTCATCAGGCCGTGCTTCG

Product: putative aromatic compound hydroxylase

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-hydroxylase; 4-HPA 3-monooxygenase large component [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MRMGIRTGAQYISGLKSRKPEIWLSGRRVINVCEEPVFKQPIREIARLYDMQHDPEYQDKITHICTETGERVSNAFLVPK
SREDLLARRALFEVWARATFGLMGRTPDFLNVVLTSLYSNASFLEKYNPQWAENIRAYYRYVRDNDLFLTHAIINPQNDR
SKPSHEQQDTFTHLGVVRETPEGLIVRGAKMLATLAPITDEVIIYTFPGYKPGDERYAVSFAIPIDTPGLRILCREPMQD
GTRPLFDHPLASRFEEMDALLVFNDVLVPWDRVFIYNNVEAANLLYPKTGIAQQPAHQTGVRGLIKLQFATEVAIRLADS
IGVDVYLNVQNDLGELLQSVEAIRALLHLAEHELEVLPSGEVMPGWVPLETIRGLLPKLYPRAVEVLQIIGAGGLLMSPT
GADFANPELAADMEKYYAGRIGVGGEERVRLFKLAWDLCGEAFGQRLLQYERFYTGDPIRKRAIFYNNIKRERTLVMVDE
ALRMPNQQEKVVNA

Sequences:

>Translated_494_residues
MRMGIRTGAQYISGLKSRKPEIWLSGRRVINVCEEPVFKQPIREIARLYDMQHDPEYQDKITHICTETGERVSNAFLVPK
SREDLLARRALFEVWARATFGLMGRTPDFLNVVLTSLYSNASFLEKYNPQWAENIRAYYRYVRDNDLFLTHAIINPQNDR
SKPSHEQQDTFTHLGVVRETPEGLIVRGAKMLATLAPITDEVIIYTFPGYKPGDERYAVSFAIPIDTPGLRILCREPMQD
GTRPLFDHPLASRFEEMDALLVFNDVLVPWDRVFIYNNVEAANLLYPKTGIAQQPAHQTGVRGLIKLQFATEVAIRLADS
IGVDVYLNVQNDLGELLQSVEAIRALLHLAEHELEVLPSGEVMPGWVPLETIRGLLPKLYPRAVEVLQIIGAGGLLMSPT
GADFANPELAADMEKYYAGRIGVGGEERVRLFKLAWDLCGEAFGQRLLQYERFYTGDPIRKRAIFYNNIKRERTLVMVDE
ALRMPNQQEKVVNA
>Mature_494_residues
MRMGIRTGAQYISGLKSRKPEIWLSGRRVINVCEEPVFKQPIREIARLYDMQHDPEYQDKITHICTETGERVSNAFLVPK
SREDLLARRALFEVWARATFGLMGRTPDFLNVVLTSLYSNASFLEKYNPQWAENIRAYYRYVRDNDLFLTHAIINPQNDR
SKPSHEQQDTFTHLGVVRETPEGLIVRGAKMLATLAPITDEVIIYTFPGYKPGDERYAVSFAIPIDTPGLRILCREPMQD
GTRPLFDHPLASRFEEMDALLVFNDVLVPWDRVFIYNNVEAANLLYPKTGIAQQPAHQTGVRGLIKLQFATEVAIRLADS
IGVDVYLNVQNDLGELLQSVEAIRALLHLAEHELEVLPSGEVMPGWVPLETIRGLLPKLYPRAVEVLQIIGAGGLLMSPT
GADFANPELAADMEKYYAGRIGVGGEERVRLFKLAWDLCGEAFGQRLLQYERFYTGDPIRKRAIFYNNIKRERTLVMVDE
ALRMPNQQEKVVNA

Specific function: Utilizes FADH(2) supplied by hpaC, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA) [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012687 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: 1.14.13.3

Molecular weight: Translated: 56164; Mature: 56164

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMGIRTGAQYISGLKSRKPEIWLSGRRVINVCEEPVFKQPIREIARLYDMQHDPEYQDK
CCCCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHH
ITHICTETGERVSNAFLVPKSREDLLARRALFEVWARATFGLMGRTPDFLNVVLTSLYSN
HHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
ASFLEKYNPQWAENIRAYYRYVRDNDLFLTHAIINPQNDRSKPSHEQQDTFTHLGVVRET
HHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
PEGLIVRGAKMLATLAPITDEVIIYTFPGYKPGDERYAVSFAIPIDTPGLRILCREPMQD
CCCCEEHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCC
GTRPLFDHPLASRFEEMDALLVFNDVLVPWDRVFIYNNVEAANLLYPKTGIAQQPAHQTG
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEECCCCCCCCCCCHHHC
VRGLIKLQFATEVAIRLADSIGVDVYLNVQNDLGELLQSVEAIRALLHLAEHELEVLPSG
CCCEEEEHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EVMPGWVPLETIRGLLPKLYPRAVEVLQIIGAGGLLMSPTGADFANPELAADMEKYYAGR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHCCC
IGVGGEERVRLFKLAWDLCGEAFGQRLLQYERFYTGDPIRKRAIFYNNIKRERTLVMVDE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEHH
ALRMPNQQEKVVNA
HHCCCCHHHHHCCC
>Mature Secondary Structure
MRMGIRTGAQYISGLKSRKPEIWLSGRRVINVCEEPVFKQPIREIARLYDMQHDPEYQDK
CCCCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHH
ITHICTETGERVSNAFLVPKSREDLLARRALFEVWARATFGLMGRTPDFLNVVLTSLYSN
HHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
ASFLEKYNPQWAENIRAYYRYVRDNDLFLTHAIINPQNDRSKPSHEQQDTFTHLGVVRET
HHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
PEGLIVRGAKMLATLAPITDEVIIYTFPGYKPGDERYAVSFAIPIDTPGLRILCREPMQD
CCCCEEHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCC
GTRPLFDHPLASRFEEMDALLVFNDVLVPWDRVFIYNNVEAANLLYPKTGIAQQPAHQTG
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEECCCCCCCCCCCHHHC
VRGLIKLQFATEVAIRLADSIGVDVYLNVQNDLGELLQSVEAIRALLHLAEHELEVLPSG
CCCEEEEHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EVMPGWVPLETIRGLLPKLYPRAVEVLQIIGAGGLLMSPTGADFANPELAADMEKYYAGR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHCCC
IGVGGEERVRLFKLAWDLCGEAFGQRLLQYERFYTGDPIRKRAIFYNNIKRERTLVMVDE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEHH
ALRMPNQQEKVVNA
HHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA