Click here to switch to the map view.

The map label for this gene is pykA [H]

Identifier: 138896300

GI number: 138896300

Start: 2771815

End: 2773578

Strand: Reverse

Name: pykA [H]

Synonym: GTNG_2663

Alternate gene names: 138896300

Gene position: 2773578-2771815 (Counterclockwise)

Preceding gene: 138896301

Following gene: 138896298

Centisome position: 78.12

GC content: 53.51

Gene sequence:

>1764_bases
ATGAAGCGGAAAACGAAAATCGTCTGTACCATTGGGCCGGCAAGTGAAAGTGTGGACAAGCTTGAACAACTGATTGAAGC
GGGAATGAACGTAGCACGCCTGAATTTTTCGCATGGCGATCACGAGGAACATGGACGGCGCATTGCCAACATTCGTGAGG
CAGCGCAGCGAACGGGCAAAACGGTCGCCATTTTGCTTGATACGAAAGGCCCGGAAATCCGGACGCACAATATGGAAAAC
GGCGCGATCGAACTGAGAGAAGGAGCGAAGCTGGTCATTTCGATGAGCGAAGTGCTCGGCACGCCAGAAAAGATCTCGGT
CACTTATCCGGGATTGATCGATGACGTATCTGTTGGTTCGAAAATTTTGCTCGACGATGGGTTGATCGCCCTTGAGGTCA
ATGCAGTTGACAAGCAAGCCGGAGAAATTATCACGACCGTTTTAAACGGCGGCGTGTTGAAAAACAAAAAAGGGGTCAAC
GTCCCGGGGGTGCGCGTCAACTTGCCGGGGATTACCGAGAAAGACCGGGCCGATATTTTGTTTGGCATTCGTCAAGGAAT
CGACTTTATCGCTGCCTCGTTCGTTCGTCGAGCGTCCGATGTGCTCGAAATTCGCGAGCTGCTTGAAGCGAACGATGCCC
TCCATATCCAAATTATCGCCAAAATTGAGAATGAAGAAGGCGTCGACAACATCGACGAAATTTTAGAAGCAGCCGACGGC
CTCATGGTGGCCCGCGGTGATTTAGGCGTCGAAATTCCGGCTGAGGAAGTGCCGCTCATCCAAAAGTTGTTGATCAAAAA
GTGCAATATGCTCGGTAAGCCGGTCATTACAGCGACGCAAATGCTTGATTCGATGCAGCGCAACCCGCGGCCGACGCGGG
CGGAAGCAAGCGACGTCGCCAACGCCATTTTTGACGGCACAGATGCTGTCATGCTTTCTGGGGAAACGGCGGCTGGTCAA
TATCCAGTTGAAGCGGTCAGAACGATGCACCAAATTGCACTCCGCACCGAACAGGCGCTTGAGCACCACGATATTTTGTC
CCAGCGCACAAAAGAAAGCGCGACGACGATTACCGATGCCATCGGACAGTCAGTCGCTCATACAGCCTTAAATTTAGATG
TAGCAGCGATCGTCACGCCGACGGTGAGCGGGAAAACGCCGCAAATGGTGGCGAAATACCGCCCGAAAGCACCGATTATT
GCTGTCACCTCAAACGAAGCGGTATCGCGCCGGTTGGCGCTCGTATGGGGCGTCTATACGAAAGAAGCGCGGCATGTGAA
CACGACGGATGAAATGCTCGATGTCGCCGTCGATGCCGCGGTGCGTTCCGGTTTGGTGAAGCACGGTGATTTAGTCGTCA
TCACCGCCGGGGTTCCGGTCGGTGAGACCGGTTCGACGAACTTAATGAAAGTGCACGTCATCAGCGACTTGATCGCGAAA
GGACAAGGCATCGGCCGCAAGTCGGCGTTTGGCAAGGCCGTTGTGGCGAAAACAGCGGAAGAAGCGTGTCAAAAAATGGT
CGAGGGTGGCATTTTAGTCACCATCAACACCGATGCCGATATGATGCCGGCAATTGAAAAGGCGTCGGCGATCATTACCG
AGGAAGGCGGATTGACAAGCCATGCCGCGGTTGTTGGCCTAAGCCTTGGCATTCCTGTCGTTGTCGGGGTGAACAATGCA
ACAAGCTTGTTTAAAGACGGGCAAGAAATTACGGTCGACGGAGGGTTTGGTGCTGTCTACCGCGGCCACGCAAGCGTCTT
GTAA

Upstream 100 bases:

>100_bases
TGCCGAAGCGTTAGCGCAGAAACATACCGTCGATCAGCGGATGTACTTACTGTCGAAAGAATTGTCCATCTAATTCCCGA
GGGGAAAGGAAGAGGAAACG

Downstream 100 bases:

>100_bases
TATCGTTTTTTATGATCGAAAAAACAGCCTTGGCCATAAAGCCAAGGCTGTTTTTATGAGGAGGGTTTACCGATGATAAA
ACGCCAAATGTCGTGAAAGA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MKRKTKIVCTIGPASESVDKLEQLIEAGMNVARLNFSHGDHEEHGRRIANIREAAQRTGKTVAILLDTKGPEIRTHNMEN
GAIELREGAKLVISMSEVLGTPEKISVTYPGLIDDVSVGSKILLDDGLIALEVNAVDKQAGEIITTVLNGGVLKNKKGVN
VPGVRVNLPGITEKDRADILFGIRQGIDFIAASFVRRASDVLEIRELLEANDALHIQIIAKIENEEGVDNIDEILEAADG
LMVARGDLGVEIPAEEVPLIQKLLIKKCNMLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAVMLSGETAAGQ
YPVEAVRTMHQIALRTEQALEHHDILSQRTKESATTITDAIGQSVAHTALNLDVAAIVTPTVSGKTPQMVAKYRPKAPII
AVTSNEAVSRRLALVWGVYTKEARHVNTTDEMLDVAVDAAVRSGLVKHGDLVVITAGVPVGETGSTNLMKVHVISDLIAK
GQGIGRKSAFGKAVVAKTAEEACQKMVEGGILVTINTDADMMPAIEKASAIITEEGGLTSHAAVVGLSLGIPVVVGVNNA
TSLFKDGQEITVDGGFGAVYRGHASVL

Sequences:

>Translated_587_residues
MKRKTKIVCTIGPASESVDKLEQLIEAGMNVARLNFSHGDHEEHGRRIANIREAAQRTGKTVAILLDTKGPEIRTHNMEN
GAIELREGAKLVISMSEVLGTPEKISVTYPGLIDDVSVGSKILLDDGLIALEVNAVDKQAGEIITTVLNGGVLKNKKGVN
VPGVRVNLPGITEKDRADILFGIRQGIDFIAASFVRRASDVLEIRELLEANDALHIQIIAKIENEEGVDNIDEILEAADG
LMVARGDLGVEIPAEEVPLIQKLLIKKCNMLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAVMLSGETAAGQ
YPVEAVRTMHQIALRTEQALEHHDILSQRTKESATTITDAIGQSVAHTALNLDVAAIVTPTVSGKTPQMVAKYRPKAPII
AVTSNEAVSRRLALVWGVYTKEARHVNTTDEMLDVAVDAAVRSGLVKHGDLVVITAGVPVGETGSTNLMKVHVISDLIAK
GQGIGRKSAFGKAVVAKTAEEACQKMVEGGILVTINTDADMMPAIEKASAIITEEGGLTSHAAVVGLSLGIPVVVGVNNA
TSLFKDGQEITVDGGFGAVYRGHASVL
>Mature_587_residues
MKRKTKIVCTIGPASESVDKLEQLIEAGMNVARLNFSHGDHEEHGRRIANIREAAQRTGKTVAILLDTKGPEIRTHNMEN
GAIELREGAKLVISMSEVLGTPEKISVTYPGLIDDVSVGSKILLDDGLIALEVNAVDKQAGEIITTVLNGGVLKNKKGVN
VPGVRVNLPGITEKDRADILFGIRQGIDFIAASFVRRASDVLEIRELLEANDALHIQIIAKIENEEGVDNIDEILEAADG
LMVARGDLGVEIPAEEVPLIQKLLIKKCNMLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAVMLSGETAAGQ
YPVEAVRTMHQIALRTEQALEHHDILSQRTKESATTITDAIGQSVAHTALNLDVAAIVTPTVSGKTPQMVAKYRPKAPII
AVTSNEAVSRRLALVWGVYTKEARHVNTTDEMLDVAVDAAVRSGLVKHGDLVVITAGVPVGETGSTNLMKVHVISDLIAK
GQGIGRKSAFGKAVVAKTAEEACQKMVEGGILVTINTDADMMPAIEKASAIITEEGGLTSHAAVVGLSLGIPVVVGVNNA
TSLFKDGQEITVDGGFGAVYRGHASVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=493, Percent_Identity=45.0304259634888, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI10835121, Length=493, Percent_Identity=45.0304259634888, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI33286422, Length=490, Percent_Identity=46.9387755102041, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI33286420, Length=490, Percent_Identity=46.9387755102041, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI33286418, Length=490, Percent_Identity=46.9387755102041, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=51.3793103448276, Blast_Score=268, Evalue=8e-72,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=51.3793103448276, Blast_Score=268, Evalue=8e-72,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=47.8991596638655, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=47.8991596638655, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI310128738, Length=217, Percent_Identity=46.5437788018433, Blast_Score=173, Evalue=4e-43,
Organism=Escherichia coli, GI1787965, Length=474, Percent_Identity=51.6877637130802, Blast_Score=475, Evalue=1e-135,
Organism=Escherichia coli, GI1788160, Length=476, Percent_Identity=41.3865546218487, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1787994, Length=113, Percent_Identity=41.5929203539823, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17544584, Length=485, Percent_Identity=47.0103092783505, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI71984413, Length=485, Percent_Identity=45.1546391752577, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=45.1546391752577, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17506829, Length=485, Percent_Identity=45.1546391752577, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17506831, Length=485, Percent_Identity=45.1546391752577, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17564524, Length=129, Percent_Identity=34.8837209302326, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319279, Length=487, Percent_Identity=42.0944558521561, Blast_Score=345, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6324923, Length=486, Percent_Identity=41.7695473251029, Blast_Score=330, Evalue=3e-91,
Organism=Drosophila melanogaster, GI24648964, Length=488, Percent_Identity=45.0819672131148, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI28571814, Length=488, Percent_Identity=45.0819672131148, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24648966, Length=407, Percent_Identity=46.6830466830467, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24581235, Length=487, Percent_Identity=36.9609856262834, Blast_Score=301, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24646914, Length=265, Percent_Identity=44.1509433962264, Blast_Score=212, Evalue=7e-55,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 62334; Mature: 62334

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKTKIVCTIGPASESVDKLEQLIEAGMNVARLNFSHGDHEEHGRRIANIREAAQRTGK
CCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
TVAILLDTKGPEIRTHNMENGAIELREGAKLVISMSEVLGTPEKISVTYPGLIDDVSVGS
EEEEEEECCCCCEEEECCCCCCEEECCCCEEEEEEHHHHCCCCEEEEECCCCCCCCCCCC
KILLDDGLIALEVNAVDKQAGEIITTVLNGGVLKNKKGVNVPGVRVNLPGITEKDRADIL
EEEEECCEEEEEEECCHHHHHHHHHHHHCCCEEECCCCCCCCCEEEECCCCCCCCHHHHH
FGIRQGIDFIAASFVRRASDVLEIRELLEANDALHIQIIAKIENEEGVDNIDEILEAADG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHCCC
LMVARGDLGVEIPAEEVPLIQKLLIKKCNMLGKPVITATQMLDSMQRNPRPTRAEASDVA
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHH
NAIFDGTDAVMLSGETAAGQYPVEAVRTMHQIALRTEQALEHHDILSQRTKESATTITDA
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGQSVAHTALNLDVAAIVTPTVSGKTPQMVAKYRPKAPIIAVTSNEAVSRRLALVWGVYT
HHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHCCCCCCEEEEECCHHHHHHHHHHEEHHH
KEARHVNTTDEMLDVAVDAAVRSGLVKHGDLVVITAGVPVGETGSTNLMKVHVISDLIAK
HCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEHHHHHHHHC
GQGIGRKSAFGKAVVAKTAEEACQKMVEGGILVTINTDADMMPAIEKASAIITEEGGLTS
CCCCCCHHHHCHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHEEEECCCCCCH
HAAVVGLSLGIPVVVGVNNATSLFKDGQEITVDGGFGAVYRGHASVL
HHHHEEEECCCEEEEECCCHHHHHCCCCEEEEECCCCEEECCCCCCC
>Mature Secondary Structure
MKRKTKIVCTIGPASESVDKLEQLIEAGMNVARLNFSHGDHEEHGRRIANIREAAQRTGK
CCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
TVAILLDTKGPEIRTHNMENGAIELREGAKLVISMSEVLGTPEKISVTYPGLIDDVSVGS
EEEEEEECCCCCEEEECCCCCCEEECCCCEEEEEEHHHHCCCCEEEEECCCCCCCCCCCC
KILLDDGLIALEVNAVDKQAGEIITTVLNGGVLKNKKGVNVPGVRVNLPGITEKDRADIL
EEEEECCEEEEEEECCHHHHHHHHHHHHCCCEEECCCCCCCCCEEEECCCCCCCCHHHHH
FGIRQGIDFIAASFVRRASDVLEIRELLEANDALHIQIIAKIENEEGVDNIDEILEAADG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHCCC
LMVARGDLGVEIPAEEVPLIQKLLIKKCNMLGKPVITATQMLDSMQRNPRPTRAEASDVA
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHH
NAIFDGTDAVMLSGETAAGQYPVEAVRTMHQIALRTEQALEHHDILSQRTKESATTITDA
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGQSVAHTALNLDVAAIVTPTVSGKTPQMVAKYRPKAPIIAVTSNEAVSRRLALVWGVYT
HHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHCCCCCCEEEEECCHHHHHHHHHHEEHHH
KEARHVNTTDEMLDVAVDAAVRSGLVKHGDLVVITAGVPVGETGSTNLMKVHVISDLIAK
HCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEHHHHHHHHC
GQGIGRKSAFGKAVVAKTAEEACQKMVEGGILVTINTDADMMPAIEKASAIITEEGGLTS
CCCCCCHHHHCHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHEEEECCCCCCH
HAAVVGLSLGIPVVVGVNNATSLFKDGQEITVDGGFGAVYRGHASVL
HHHHEEEECCCEEEEECCCHHHHHCCCCEEEEECCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436141; 1447787 [H]