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The map label for this gene is phoA [H]

Identifier: 138896265

GI number: 138896265

Start: 2737090

End: 2738364

Strand: Direct

Name: phoA [H]

Synonym: GTNG_2628

Alternate gene names: 138896265

Gene position: 2737090-2738364 (Clockwise)

Preceding gene: 138896262

Following gene: 138896273

Centisome position: 77.09

GC content: 51.76

Gene sequence:

>1275_bases
ATGTTCAAATCGAAACGCTGCGCTGCTCTACTTATCGCTACGCTAAGTGTCGGACTCACGCTCAACCATGGCGGACAAGC
AATATCGGCTGCACCGTCCAAGCCCGCAAAGCCACCTGTCGTGAAAAATGTCATCATGCTCGTCGGCGACGGTATGGGAA
CAGCCCAGCGCAATGCCATTCGCCTAGCGACGAAAGGGATAGACGGGGAACTAGAAATGGACAGCATGCCATACAGCGGG
CTCATTCATACAAACTCCGCCGACCCCAAATCATTCATCACCGACTCCGCTGCGGCTGCCAGCGCGATAGCGACAGGTGT
AAAAACGTATAACGGCGCCATCTCCGTTGACTTACAAGGAAAACCGGTGAAAACGATTTTGGAACAAGCGAAAAAAGCCG
GGAAAGCGACCGGGCTTGTCACGACTGCCCAAGTAACCGATGCTACCCCAGCGGCATTCGCTGCTCACACCGCCAATCGC
TCCGCACAAAGCGACATCGCCAAGCAATACATCGAAAAAACCAAAGTTGACGTCATTTTGGGCGGCGGGGAAGACTACTG
GTATCCAGCTGGACATCCTGGCTATTACCCAGATACGGCTGAAGATGCAGAAGAAGGAAGCAAAGGCAAGCAAGGAAACT
TAGTTGAACGCGCCAAGAAGCTCGGTTACACCTATGTCCGCACCGCCGATGAGCTGAAACGGGCAAAGGGGCGCAAACTG
CTTGGATTATTCGCCAATGAAGAAATGTTCCAAAAACGTTCCGAAGGAGAAGGTAAGTACAACCCCGTCGTATCGCTGCC
TGATATGACAAAAAAAGCGATTGATGTGTTGTCGAAAAACAAAAAAGGCTTTTTCCTTGTCGTCGAAGAGGAAGCGATTG
ATGAAATGAGCCATGACAATAACGGTTCGCTCATGATCAAAGCGGGGCAACAGTTTGATCAAGCAGTCGCCGTGGCGAAA
CGGTACGCCAAGCACCATCCAGACACACTCGTTCTTGTGCTCGCCGACCACGAATCCGGCGGCCTGACGATTGAAACGCC
GGGCGACGCAGATGAATCAGAAGACAGTAACACGCTCTCTGACGAAAACGGCCCGTTTGCCGTCGCCCATTCTAAACAAA
CATTCACCTTAAACTGGACGACAACAGGCCACACTGCTGCCGGTGTGCCACTGACCGCCATGGGTCCGGGAGCGAAACGG
TTTGCTGGCGTGTATGAAAATACGCACATTCATGACATTCTTGAGACGTTATTGTTTGGAAAAAGAGAAAGATAA

Upstream 100 bases:

>100_bases
GGTTTATTTGCAAAACGATTACATAGAATTCACATTCTCTTCATATCGAGCTTGTACGATAAACATGACAAAATGAATTT
GAAAAAAGGAGAGGAGAACG

Downstream 100 bases:

>100_bases
CCGATGCGTTATTTCCATCGTTCCCCTTCATCCGCTCTCTCTTCCCCGCCAATGTGTCGTTGACGGGGAGAGAGTTTTTG
TCGATTTTTCCAGTCTTCGA

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MFKSKRCAALLIATLSVGLTLNHGGQAISAAPSKPAKPPVVKNVIMLVGDGMGTAQRNAIRLATKGIDGELEMDSMPYSG
LIHTNSADPKSFITDSAAAASAIATGVKTYNGAISVDLQGKPVKTILEQAKKAGKATGLVTTAQVTDATPAAFAAHTANR
SAQSDIAKQYIEKTKVDVILGGGEDYWYPAGHPGYYPDTAEDAEEGSKGKQGNLVERAKKLGYTYVRTADELKRAKGRKL
LGLFANEEMFQKRSEGEGKYNPVVSLPDMTKKAIDVLSKNKKGFFLVVEEEAIDEMSHDNNGSLMIKAGQQFDQAVAVAK
RYAKHHPDTLVLVLADHESGGLTIETPGDADESEDSNTLSDENGPFAVAHSKQTFTLNWTTTGHTAAGVPLTAMGPGAKR
FAGVYENTHIHDILETLLFGKRER

Sequences:

>Translated_424_residues
MFKSKRCAALLIATLSVGLTLNHGGQAISAAPSKPAKPPVVKNVIMLVGDGMGTAQRNAIRLATKGIDGELEMDSMPYSG
LIHTNSADPKSFITDSAAAASAIATGVKTYNGAISVDLQGKPVKTILEQAKKAGKATGLVTTAQVTDATPAAFAAHTANR
SAQSDIAKQYIEKTKVDVILGGGEDYWYPAGHPGYYPDTAEDAEEGSKGKQGNLVERAKKLGYTYVRTADELKRAKGRKL
LGLFANEEMFQKRSEGEGKYNPVVSLPDMTKKAIDVLSKNKKGFFLVVEEEAIDEMSHDNNGSLMIKAGQQFDQAVAVAK
RYAKHHPDTLVLVLADHESGGLTIETPGDADESEDSNTLSDENGPFAVAHSKQTFTLNWTTTGHTAAGVPLTAMGPGAKR
FAGVYENTHIHDILETLLFGKRER
>Mature_424_residues
MFKSKRCAALLIATLSVGLTLNHGGQAISAAPSKPAKPPVVKNVIMLVGDGMGTAQRNAIRLATKGIDGELEMDSMPYSG
LIHTNSADPKSFITDSAAAASAIATGVKTYNGAISVDLQGKPVKTILEQAKKAGKATGLVTTAQVTDATPAAFAAHTANR
SAQSDIAKQYIEKTKVDVILGGGEDYWYPAGHPGYYPDTAEDAEEGSKGKQGNLVERAKKLGYTYVRTADELKRAKGRKL
LGLFANEEMFQKRSEGEGKYNPVVSLPDMTKKAIDVLSKNKKGFFLVVEEEAIDEMSHDNNGSLMIKAGQQFDQAVAVAK
RYAKHHPDTLVLVLADHESGGLTIETPGDADESEDSNTLSDENGPFAVAHSKQTFTLNWTTTGHTAAGVPLTAMGPGAKR
FAGVYENTHIHDILETLLFGKRER

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266292, Length=445, Percent_Identity=31.685393258427, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI157266296, Length=437, Percent_Identity=31.8077803203661, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI94721246, Length=437, Percent_Identity=31.350114416476, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI116734717, Length=340, Percent_Identity=33.5294117647059, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI294660770, Length=334, Percent_Identity=32.6347305389222, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI294660772, Length=303, Percent_Identity=32.6732673267327, Blast_Score=108, Evalue=9e-24,
Organism=Escherichia coli, GI48994877, Length=343, Percent_Identity=37.9008746355685, Blast_Score=162, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6320689, Length=363, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21355981, Length=451, Percent_Identity=29.7117516629712, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI21355151, Length=332, Percent_Identity=31.6265060240964, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI18859923, Length=415, Percent_Identity=29.1566265060241, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21355149, Length=441, Percent_Identity=29.4784580498866, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI19921912, Length=446, Percent_Identity=29.372197309417, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI85725200, Length=448, Percent_Identity=28.5714285714286, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI85815833, Length=448, Percent_Identity=28.5714285714286, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24585213, Length=365, Percent_Identity=32.3287671232877, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI21358067, Length=463, Percent_Identity=28.5097192224622, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24651554, Length=462, Percent_Identity=27.7056277056277, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24657835, Length=339, Percent_Identity=28.9085545722714, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24657842, Length=344, Percent_Identity=28.7790697674419, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24651556, Length=426, Percent_Identity=27.6995305164319, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24657827, Length=343, Percent_Identity=31.7784256559767, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 45232; Mature: 45232

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKSKRCAALLIATLSVGLTLNHGGQAISAAPSKPAKPPVVKNVIMLVGDGMGTAQRNAI
CCCCHHHHHHHHHHHHHCEEECCCCCEEECCCCCCCCCHHHHHEEEEEECCCCCCCCCEE
RLATKGIDGELEMDSMPYSGLIHTNSADPKSFITDSAAAASAIATGVKTYNGAISVDLQG
EEEECCCCCCEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
KPVKTILEQAKKAGKATGLVTTAQVTDATPAAFAAHTANRSAQSDIAKQYIEKTKVDVIL
CCHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHCCEEEEE
GGGEDYWYPAGHPGYYPDTAEDAEEGSKGKQGNLVERAKKLGYTYVRTADELKRAKGRKL
ECCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHCCCCEEECHHHHHHHCCCEE
LGLFANEEMFQKRSEGEGKYNPVVSLPDMTKKAIDVLSKNKKGFFLVVEEEAIDEMSHDN
EEEECCHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHCCCC
NGSLMIKAGQQFDQAVAVAKRYAKHHPDTLVLVLADHESGGLTIETPGDADESEDSNTLS
CCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCCCCCCCCCCCC
DENGPFAVAHSKQTFTLNWTTTGHTAAGVPLTAMGPGAKRFAGVYENTHIHDILETLLFG
CCCCCEEEEECCEEEEEEEEECCCCCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHCC
KRER
CCCC
>Mature Secondary Structure
MFKSKRCAALLIATLSVGLTLNHGGQAISAAPSKPAKPPVVKNVIMLVGDGMGTAQRNAI
CCCCHHHHHHHHHHHHHCEEECCCCCEEECCCCCCCCCHHHHHEEEEEECCCCCCCCCEE
RLATKGIDGELEMDSMPYSGLIHTNSADPKSFITDSAAAASAIATGVKTYNGAISVDLQG
EEEECCCCCCEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
KPVKTILEQAKKAGKATGLVTTAQVTDATPAAFAAHTANRSAQSDIAKQYIEKTKVDVIL
CCHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHCCEEEEE
GGGEDYWYPAGHPGYYPDTAEDAEEGSKGKQGNLVERAKKLGYTYVRTADELKRAKGRKL
ECCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHCCCCEEECHHHHHHHCCCEE
LGLFANEEMFQKRSEGEGKYNPVVSLPDMTKKAIDVLSKNKKGFFLVVEEEAIDEMSHDN
EEEECCHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHCCCC
NGSLMIKAGQQFDQAVAVAKRYAKHHPDTLVLVLADHESGGLTIETPGDADESEDSNTLS
CCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCCCCCCCCCCCC
DENGPFAVAHSKQTFTLNWTTTGHTAAGVPLTAMGPGAKRFAGVYENTHIHDILETLLFG
CCCCCEEEEECCEEEEEEEEECCCCCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHCC
KRER
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]