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The map label for this gene is aspA [H]

Identifier: 138895916

GI number: 138895916

Start: 2392437

End: 2393873

Strand: Reverse

Name: aspA [H]

Synonym: GTNG_2275

Alternate gene names: 138895916

Gene position: 2393873-2392437 (Counterclockwise)

Preceding gene: 138895917

Following gene: 138895915

Centisome position: 67.43

GC content: 41.54

Gene sequence:

>1437_bases
ATGGCAACCGCTAATACCAATAGTAAGGTTCGGATTGAAAGGGATTTCCTAGGGGATAAAGAAGTTCCGATAGATGCTTA
TTATGGGATACAAACATTAAGAGCCCTAGAGAACTTTCCTATCACCGGATATCGAATTCACAAGGAATTGATTAAAGCTT
TGGCGATGGTGAAGAAAGCAGCTGCCTTAGCCAATATGGACACAAAACGATTAAAAGCTGAAATAGGCGAGGCCATCGTT
AAAGCTGCAGATGAAATCATTGCAGGAAAATGGCATGAGCACTTTATCGTTGATCCTATACAAGGCGGAGCGGGTACATC
GATAAATATGAATGCAAATGAAGTCATCGCCAATCGTGCTCTCGAATTGCTTGGATATGAAAAAGGAGAATATGGTTATT
TAAGTCCAAATAATCATGTGAATATGTCTCAGTCAACCAACGATGTTTTCCCAACAGCGGTTCATATTGCCTTACTAAAC
TTACTAGACAAACTGCTCCATACGATGCGATATATGCATGCAGTGATGAAGGAAAAAGCCCGGCAATTTGACCATGTCAT
TAAAATAGGCCGCACCCATCTTCAAGATGCTGTACCCATTCGTTTAGGGCAAGAGTTTGAAGCATATAGCTGCGTGTTAG
AGCGTGATATCCAACGCATCGATCGCTCCCGTCAATATTTATATGAAATCAATATGGGAGCCACCGCAGTCGGGACGGGG
TTAAATGCTGATCCCCGATATATTGAGGGTGTTGTCAAATATTTATCAGAAATTAGCGGGTTGCCATTAGTTAGAGCTGA
ACATTTGGTGGATGCAACACAAAATACGGATGCATATACAGAAGTATCGTCCGCTCTAAAAGTGTGTATGATCAATATGT
CTAAAATAGCCAATGACTTGCGTTTAATGGCGTCAGGACCTAGAGCCGGTTTGGGAGAAATTACATTGCCGCCCCGTCAG
CCTGGCTCGTCTATTATGCCAGGTAAAGTTAATCCTGTTATGGCCGAGGTGATTAACCAAGTCGCTTTCCAAGTGATAGG
CAATGACCATACGATTTGTCTTGCTTCTGAGGCAGGTCAACTGGAACTAAATGTAATGGAACCTGTGCTCGTCTTTAATC
TGCTTCAATCGATTACGATAATGACGAATGCGTTTCATGTGTTTACAAATTATTGTTTAAAGGGTATTGAAGCCAACGAG
GAACGTTTGAGACATTATGTAGAAAAAAGTGTCAGTGTGGTTACAGCTGTTAGCCCATATATCGGATATGAAACGGCCGC
ACGTATTGCTCAACAGGCGATCTTAGAAGGGGAATCCATCCGTAAACTATGTTTGCAATACGGTTTGTTAACGGAAAAAG
AATTGGATCATATTTTAGATCCTTATAAAATGACGAGTCCAGGCATCCTCAGTGTTATTTCTTTTGACAAGCCCTAA

Upstream 100 bases:

>100_bases
GATCAGTGGACATAATGTAGATCTTTCTGTGCTTTTCCAATTGATTCAGCGTTACAACGATTTGTCTCATTAAACCACTA
ACAATAAGAGGTGGGACTCG

Downstream 100 bases:

>100_bases
TTTTTTCTATTTGTAATGTGGTATTATATATTTGATACCTAGGCTTGAACAGTCAAAAGGAGGATAGCATGCTAACAGTA
GGGGATGTGCTTAAATTTGA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MATANTNSKVRIERDFLGDKEVPIDAYYGIQTLRALENFPITGYRIHKELIKALAMVKKAAALANMDTKRLKAEIGEAIV
KAADEIIAGKWHEHFIVDPIQGGAGTSINMNANEVIANRALELLGYEKGEYGYLSPNNHVNMSQSTNDVFPTAVHIALLN
LLDKLLHTMRYMHAVMKEKARQFDHVIKIGRTHLQDAVPIRLGQEFEAYSCVLERDIQRIDRSRQYLYEINMGATAVGTG
LNADPRYIEGVVKYLSEISGLPLVRAEHLVDATQNTDAYTEVSSALKVCMINMSKIANDLRLMASGPRAGLGEITLPPRQ
PGSSIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLLQSITIMTNAFHVFTNYCLKGIEANE
ERLRHYVEKSVSVVTAVSPYIGYETAARIAQQAILEGESIRKLCLQYGLLTEKELDHILDPYKMTSPGILSVISFDKP

Sequences:

>Translated_478_residues
MATANTNSKVRIERDFLGDKEVPIDAYYGIQTLRALENFPITGYRIHKELIKALAMVKKAAALANMDTKRLKAEIGEAIV
KAADEIIAGKWHEHFIVDPIQGGAGTSINMNANEVIANRALELLGYEKGEYGYLSPNNHVNMSQSTNDVFPTAVHIALLN
LLDKLLHTMRYMHAVMKEKARQFDHVIKIGRTHLQDAVPIRLGQEFEAYSCVLERDIQRIDRSRQYLYEINMGATAVGTG
LNADPRYIEGVVKYLSEISGLPLVRAEHLVDATQNTDAYTEVSSALKVCMINMSKIANDLRLMASGPRAGLGEITLPPRQ
PGSSIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLLQSITIMTNAFHVFTNYCLKGIEANE
ERLRHYVEKSVSVVTAVSPYIGYETAARIAQQAILEGESIRKLCLQYGLLTEKELDHILDPYKMTSPGILSVISFDKP
>Mature_477_residues
ATANTNSKVRIERDFLGDKEVPIDAYYGIQTLRALENFPITGYRIHKELIKALAMVKKAAALANMDTKRLKAEIGEAIVK
AADEIIAGKWHEHFIVDPIQGGAGTSINMNANEVIANRALELLGYEKGEYGYLSPNNHVNMSQSTNDVFPTAVHIALLNL
LDKLLHTMRYMHAVMKEKARQFDHVIKIGRTHLQDAVPIRLGQEFEAYSCVLERDIQRIDRSRQYLYEINMGATAVGTGL
NADPRYIEGVVKYLSEISGLPLVRAEHLVDATQNTDAYTEVSSALKVCMINMSKIANDLRLMASGPRAGLGEITLPPRQP
GSSIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLLQSITIMTNAFHVFTNYCLKGIEANEE
RLRHYVEKSVSVVTAVSPYIGYETAARIAQQAILEGESIRKLCLQYGLLTEKELDHILDPYKMTSPGILSVISFDKP

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=470, Percent_Identity=43.4042553191489, Blast_Score=383, Evalue=1e-106,
Organism=Escherichia coli, GI87082375, Length=464, Percent_Identity=49.3534482758621, Blast_Score=443, Evalue=1e-125,
Organism=Escherichia coli, GI1787896, Length=456, Percent_Identity=43.640350877193, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI1790398, Length=303, Percent_Identity=23.1023102310231, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17553882, Length=463, Percent_Identity=44.7084233261339, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=43.1192660550459, Blast_Score=287, Evalue=7e-78,
Organism=Saccharomyces cerevisiae, GI6324993, Length=464, Percent_Identity=43.9655172413793, Blast_Score=395, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24640179, Length=463, Percent_Identity=42.7645788336933, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24640177, Length=463, Percent_Identity=42.7645788336933, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24662684, Length=464, Percent_Identity=43.1034482758621, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI78710009, Length=462, Percent_Identity=41.3419913419913, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24583245, Length=463, Percent_Identity=39.524838012959, Blast_Score=343, Evalue=2e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 53010; Mature: 52879

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATANTNSKVRIERDFLGDKEVPIDAYYGIQTLRALENFPITGYRIHKELIKALAMVKKA
CCCCCCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AALANMDTKRLKAEIGEAIVKAADEIIAGKWHEHFIVDPIQGGAGTSINMNANEVIANRA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEECCCHHHHHHHHH
LELLGYEKGEYGYLSPNNHVNMSQSTNDVFPTAVHIALLNLLDKLLHTMRYMHAVMKEKA
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQFDHVIKIGRTHLQDAVPIRLGQEFEAYSCVLERDIQRIDRSRQYLYEINMGATAVGTG
HHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEECCC
LNADPRYIEGVVKYLSEISGLPLVRAEHLVDATQNTDAYTEVSSALKVCMINMSKIANDL
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RLMASGPRAGLGEITLPPRQPGSSIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQ
HHHCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCC
LELNVMEPVLVFNLLQSITIMTNAFHVFTNYCLKGIEANEERLRHYVEKSVSVVTAVSPY
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IGYETAARIAQQAILEGESIRKLCLQYGLLTEKELDHILDPYKMTSPGILSVISFDKP
HCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
ATANTNSKVRIERDFLGDKEVPIDAYYGIQTLRALENFPITGYRIHKELIKALAMVKKA
CCCCCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AALANMDTKRLKAEIGEAIVKAADEIIAGKWHEHFIVDPIQGGAGTSINMNANEVIANRA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEECCCHHHHHHHHH
LELLGYEKGEYGYLSPNNHVNMSQSTNDVFPTAVHIALLNLLDKLLHTMRYMHAVMKEKA
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQFDHVIKIGRTHLQDAVPIRLGQEFEAYSCVLERDIQRIDRSRQYLYEINMGATAVGTG
HHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEECCC
LNADPRYIEGVVKYLSEISGLPLVRAEHLVDATQNTDAYTEVSSALKVCMINMSKIANDL
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RLMASGPRAGLGEITLPPRQPGSSIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQ
HHHCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCC
LELNVMEPVLVFNLLQSITIMTNAFHVFTNYCLKGIEANEERLRHYVEKSVSVVTAVSPY
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IGYETAARIAQQAILEGESIRKLCLQYGLLTEKELDHILDPYKMTSPGILSVISFDKP
HCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]