Click here to switch to the map view.

The map label for this gene is mdeA [H]

Identifier: 138895911

GI number: 138895911

Start: 2386251

End: 2387462

Strand: Reverse

Name: mdeA [H]

Synonym: GTNG_2270

Alternate gene names: 138895911

Gene position: 2387462-2386251 (Counterclockwise)

Preceding gene: 138895912

Following gene: 138895910

Centisome position: 67.25

GC content: 42.49

Gene sequence:

>1212_bases
ATGGAGGGAAAGCATATGTTCAATGATATTAAAGTAGGAACAAAAGCAGTCTGGGCAGGAGAAAAAGAGTATTTAGTTCA
TGGAGCGACGCAAGTTCCGGTAGTTCTCAGTGTTTCTTATGGATACGACGATATTGATGAATGGTATGATGTAGCGATCG
GGAAGAAAAAAGGGCATATTTACGGACGCAATACGAATCCGACGGTTCAAGCATTTGAAGACAAAGTGAAAATTCTTGAA
GGAGCTGAAGCCGCTACTAGCTTTTCAACAGGGATGGCTGCAATTAGCAACACCTTAGCAACGTTTCTTGTTCCAGGTGA
CCGTATTGTCTCTATTAAGGACACGTATGGCGGCACAAATAAAATCTTTACCGAGTTTCTTCCACGCCAACAAATTGATG
TAGTTCTTTGCGAGACAGGCAACCACGAACAGATTGAAGAAGAAATTGCAAAAGGATGCAAAATCTTATATTTAGAAACA
CCTACGAACCCAACTGTAAAGATTACAGATATTGAACGTATGGCAAAAGCCGGCCATGAAGTTGGAGCATTGGTCATTAT
TGACAATACATTCGCCACGCCAATCAACCAAAATCCTCTTTCATTAGGTGCGGACTTAGTCATCCATAGTGCAACAAAGT
TTTTAGGGGGCCACGCAGATGCATTAGGTGGCGTTGTATGCGGGCCGAAAGATCTGATTGATAAGATTTATCACTATCGT
GAGATTAATGGAGCGACAATGGATCCGATGGCTGCATATCTCATTTTGCGCGGTATGAAAACTCTCCATCTTCGTGTGCA
GCGGCAATGCGAAAATGCGATGGCTCTTGCAAAATATTTGCAAACGAAAGATATTGTAGAAACTGTTTATTATCCTGGTC
TTGAAACGCATAAACATCATGACATTGCCAAAAAGCAAATGCGAAACTTCGGAGGAATGCTCAGTTTTGCGGTGAAAGGC
GGAATTGATACAGTGAGAGAATTTCTGCCAAAACTTCAATTTGCTAATCGAGCCGCGAACTTAGGTGCTGTAGAAACAAT
CGTTGGTCCGGCACGTACAACAAGTCATGTCGAGTGTACTCCGGAAGAGCGGGCGGCTATGGGAATTCCTGAAGGCCTCG
TTCGGGTTTCTTGCGGCATTGAAGATATTGAAGACATTATTGCCGATTTTGAGCAAGCATTTCAACATGCTGAAAGCCTT
TTGAAAATTTAA

Upstream 100 bases:

>100_bases
GGGTGCAAGATACCGCCATCGCTTTATTTGCTTACCATGAAATGAAAAAACGGAATTTGGGATTGAAGGTTGAGAATCAA
GTAGCCTTATTAAAACAATA

Downstream 100 bases:

>100_bases
CTGCAGTGCTTATCAGGGGAGTATAACAAAAAAGATGATGGAACAACTGAATGACGAACTTTTCCCATCCGTGATGGATG
GGAAAAGTTTTCAATGAGCA

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MEGKHMFNDIKVGTKAVWAGEKEYLVHGATQVPVVLSVSYGYDDIDEWYDVAIGKKKGHIYGRNTNPTVQAFEDKVKILE
GAEAATSFSTGMAAISNTLATFLVPGDRIVSIKDTYGGTNKIFTEFLPRQQIDVVLCETGNHEQIEEEIAKGCKILYLET
PTNPTVKITDIERMAKAGHEVGALVIIDNTFATPINQNPLSLGADLVIHSATKFLGGHADALGGVVCGPKDLIDKIYHYR
EINGATMDPMAAYLILRGMKTLHLRVQRQCENAMALAKYLQTKDIVETVYYPGLETHKHHDIAKKQMRNFGGMLSFAVKG
GIDTVREFLPKLQFANRAANLGAVETIVGPARTTSHVECTPEERAAMGIPEGLVRVSCGIEDIEDIIADFEQAFQHAESL
LKI

Sequences:

>Translated_403_residues
MEGKHMFNDIKVGTKAVWAGEKEYLVHGATQVPVVLSVSYGYDDIDEWYDVAIGKKKGHIYGRNTNPTVQAFEDKVKILE
GAEAATSFSTGMAAISNTLATFLVPGDRIVSIKDTYGGTNKIFTEFLPRQQIDVVLCETGNHEQIEEEIAKGCKILYLET
PTNPTVKITDIERMAKAGHEVGALVIIDNTFATPINQNPLSLGADLVIHSATKFLGGHADALGGVVCGPKDLIDKIYHYR
EINGATMDPMAAYLILRGMKTLHLRVQRQCENAMALAKYLQTKDIVETVYYPGLETHKHHDIAKKQMRNFGGMLSFAVKG
GIDTVREFLPKLQFANRAANLGAVETIVGPARTTSHVECTPEERAAMGIPEGLVRVSCGIEDIEDIIADFEQAFQHAESL
LKI
>Mature_403_residues
MEGKHMFNDIKVGTKAVWAGEKEYLVHGATQVPVVLSVSYGYDDIDEWYDVAIGKKKGHIYGRNTNPTVQAFEDKVKILE
GAEAATSFSTGMAAISNTLATFLVPGDRIVSIKDTYGGTNKIFTEFLPRQQIDVVLCETGNHEQIEEEIAKGCKILYLET
PTNPTVKITDIERMAKAGHEVGALVIIDNTFATPINQNPLSLGADLVIHSATKFLGGHADALGGVVCGPKDLIDKIYHYR
EINGATMDPMAAYLILRGMKTLHLRVQRQCENAMALAKYLQTKDIVETVYYPGLETHKHHDIAKKQMRNFGGMLSFAVKG
GIDTVREFLPKLQFANRAANLGAVETIVGPARTTSHVECTPEERAAMGIPEGLVRVSCGIEDIEDIIADFEQAFQHAESL
LKI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=388, Percent_Identity=34.020618556701, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI299473759, Length=388, Percent_Identity=30.6701030927835, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI25453487, Length=387, Percent_Identity=30.749354005168, Blast_Score=167, Evalue=1e-41,
Organism=Escherichia coli, GI1790375, Length=388, Percent_Identity=38.1443298969072, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI1789383, Length=399, Percent_Identity=25.5639097744361, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17538055, Length=390, Percent_Identity=34.8717948717949, Blast_Score=231, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI17560056, Length=389, Percent_Identity=34.7043701799486, Blast_Score=221, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI71980708, Length=369, Percent_Identity=31.9783197831978, Blast_Score=205, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6319307, Length=397, Percent_Identity=36.0201511335013, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6323333, Length=415, Percent_Identity=35.1807228915663, Blast_Score=199, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6321254, Length=397, Percent_Identity=28.9672544080605, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321904, Length=384, Percent_Identity=29.9479166666667, Blast_Score=139, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6321170, Length=295, Percent_Identity=28.135593220339, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322970, Length=257, Percent_Identity=26.0700389105058, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922554, Length=390, Percent_Identity=34.6153846153846, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 44219; Mature: 44219

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGKHMFNDIKVGTKAVWAGEKEYLVHGATQVPVVLSVSYGYDDIDEWYDVAIGKKKGHI
CCCCHHHHHHCCCCEEEECCCHHHEEECCCCCCEEEEEECCCCCHHHHHHHHCCCCCCEE
YGRNTNPTVQAFEDKVKILEGAEAATSFSTGMAAISNTLATFLVPGDRIVSIKDTYGGTN
ECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCCH
KIFTEFLPRQQIDVVLCETGNHEQIEEEIAKGCKILYLETPTNPTVKITDIERMAKAGHE
HHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCEEEECHHHHHHCCCC
VGALVIIDNTFATPINQNPLSLGADLVIHSATKFLGGHADALGGVVCGPKDLIDKIYHYR
CEEEEEEECCCCCCCCCCCHHHCHHHHHHHHHHHHCCCHHHHCCEEECCHHHHHHHHHHH
EINGATMDPMAAYLILRGMKTLHLRVQRQCENAMALAKYLQTKDIVETVYYPGLETHKHH
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
DIAKKQMRNFGGMLSFAVKGGIDTVREFLPKLQFANRAANLGAVETIVGPARTTSHVECT
HHHHHHHHHHCCCEEHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEECC
PEERAAMGIPEGLVRVSCGIEDIEDIIADFEQAFQHAESLLKI
CHHHHHCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEGKHMFNDIKVGTKAVWAGEKEYLVHGATQVPVVLSVSYGYDDIDEWYDVAIGKKKGHI
CCCCHHHHHHCCCCEEEECCCHHHEEECCCCCCEEEEEECCCCCHHHHHHHHCCCCCCEE
YGRNTNPTVQAFEDKVKILEGAEAATSFSTGMAAISNTLATFLVPGDRIVSIKDTYGGTN
ECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCCH
KIFTEFLPRQQIDVVLCETGNHEQIEEEIAKGCKILYLETPTNPTVKITDIERMAKAGHE
HHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCEEEECHHHHHHCCCC
VGALVIIDNTFATPINQNPLSLGADLVIHSATKFLGGHADALGGVVCGPKDLIDKIYHYR
CEEEEEEECCCCCCCCCCCHHHCHHHHHHHHHHHHCCCHHHHCCEEECCHHHHHHHHHHH
EINGATMDPMAAYLILRGMKTLHLRVQRQCENAMALAKYLQTKDIVETVYYPGLETHKHH
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
DIAKKQMRNFGGMLSFAVKGGIDTVREFLPKLQFANRAANLGAVETIVGPARTTSHVECT
HHHHHHHHHHCCCEEHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEECC
PEERAAMGIPEGLVRVSCGIEDIEDIIADFEQAFQHAESLLKI
CHHHHHCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]