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The map label for this gene is nudF [H]

Identifier: 138895892

GI number: 138895892

Start: 2368205

End: 2368759

Strand: Reverse

Name: nudF [H]

Synonym: GTNG_2251

Alternate gene names: 138895892

Gene position: 2368759-2368205 (Counterclockwise)

Preceding gene: 138895894

Following gene: 138895891

Centisome position: 66.72

GC content: 52.43

Gene sequence:

>555_bases
ATGGATCAGTTGTATGAAAAAACGATCCGCCAAGAAAAATTATTCAGCGGACGCATCATCGAACTGTATGTTGAAGAGGT
GGAGCTGCCAAACGGGAAAACGAGTCGGCGCGAGGTGATTAAACATCCGGGGGCGGTAGCGGTGTTGCCGCTCTTGCCCG
ATGGGAAGATCGTCCTCGTCCGCCAGTATCGAAAAGCGCTCGAGCGCGCATTGGTTGAAATTCCGGCGGGCAAGCTAGAA
CATGGGGAAGAGCCGCTCGCTTCCGCACACCGCGAACTTGAAGAAGAAACCGGCTACCGCGCCCAATCGATGCACCACCT
CATTTCGTTTTATACATCGCCCGGATTTGCCGACGAGCTCATCCATTTGTACGTCGCCGAGGGGTTGGAAAAAGTCGAAG
ATGGTGCCGGTTTAGATGAAGATGAATTTGTTGAACTGCTGGAAGTGACGTTAGAGGAGGCGCTGGATATGCTTGAACGA
CGTGACATTTATGATGCTAAAACGGCGTATGCCTTGCAATATTTGCAGCTGCGCCGCGCGCTTGAGAAACGATGA

Upstream 100 bases:

>100_bases
ACGCTCCCTTCTTAATGATCATTGTACAACAGGGGCGAAAAAATGGCCAAATATGTAGTACAATGGAAAAGAATTTATCA
ATGGATAGGGGAGAATAAGA

Downstream 100 bases:

>100_bases
CTAACGGAAAAAACGATGGCGTCCTCGGCCGCTATTACGCTGATTTACATATTCATATCGGCCGCACCGCTTCCGGCCGT
CCGGTGAAAATTACTGGGGC

Product: ADP-ribose pyrophosphatase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MDQLYEKTIRQEKLFSGRIIELYVEEVELPNGKTSRREVIKHPGAVAVLPLLPDGKIVLVRQYRKALERALVEIPAGKLE
HGEEPLASAHRELEEETGYRAQSMHHLISFYTSPGFADELIHLYVAEGLEKVEDGAGLDEDEFVELLEVTLEEALDMLER
RDIYDAKTAYALQYLQLRRALEKR

Sequences:

>Translated_184_residues
MDQLYEKTIRQEKLFSGRIIELYVEEVELPNGKTSRREVIKHPGAVAVLPLLPDGKIVLVRQYRKALERALVEIPAGKLE
HGEEPLASAHRELEEETGYRAQSMHHLISFYTSPGFADELIHLYVAEGLEKVEDGAGLDEDEFVELLEVTLEEALDMLER
RDIYDAKTAYALQYLQLRRALEKR
>Mature_184_residues
MDQLYEKTIRQEKLFSGRIIELYVEEVELPNGKTSRREVIKHPGAVAVLPLLPDGKIVLVRQYRKALERALVEIPAGKLE
HGEEPLASAHRELEEETGYRAQSMHHLISFYTSPGFADELIHLYVAEGLEKVEDGAGLDEDEFVELLEVTLEEALDMLER
RDIYDAKTAYALQYLQLRRALEKR

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI37594464, Length=169, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1788810, Length=155, Percent_Identity=36.1290322580645, Blast_Score=79, Evalue=1e-16,
Organism=Escherichia coli, GI1789412, Length=181, Percent_Identity=33.7016574585635, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 21131; Mature: 21131

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQLYEKTIRQEKLFSGRIIELYVEEVELPNGKTSRREVIKHPGAVAVLPLLPDGKIVLV
CCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCCEEHH
RQYRKALERALVEIPAGKLEHGEEPLASAHRELEEETGYRAQSMHHLISFYTSPGFADEL
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHH
IHLYVAEGLEKVEDGAGLDEDEFVELLEVTLEEALDMLERRDIYDAKTAYALQYLQLRRA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
LEKR
HHCC
>Mature Secondary Structure
MDQLYEKTIRQEKLFSGRIIELYVEEVELPNGKTSRREVIKHPGAVAVLPLLPDGKIVLV
CCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCCEEHH
RQYRKALERALVEIPAGKLEHGEEPLASAHRELEEETGYRAQSMHHLISFYTSPGFADEL
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHH
IHLYVAEGLEKVEDGAGLDEDEFVELLEVTLEEALDMLERRDIYDAKTAYALQYLQLRRA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
LEKR
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]