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The map label for this gene is dacB [H]

Identifier: 138895855

GI number: 138895855

Start: 2338298

End: 2339638

Strand: Reverse

Name: dacB [H]

Synonym: GTNG_2214

Alternate gene names: 138895855

Gene position: 2339638-2338298 (Counterclockwise)

Preceding gene: 138895856

Following gene: 138895854

Centisome position: 65.9

GC content: 52.5

Gene sequence:

>1341_bases
ATGCTAGCTTCTGTAGCCATATTTTTCATCATATTTGTCCGATCGGCGCATACACTTGTACAAAGCATGGCCTCCATGTC
CAGCCTGTTTCCGTCGCGATGTTCCCTCTGTTGCGCTCATTTCAGCCTAATTGGAAAGGAGGAACAGGCGATTCCTCCCT
GCTTACGGAAGGAGGGGGATCCTCGCCGATATCGGATGAAGAGGTGGAAGCGACTCGTTTGGCTCATTGCGCTCACTGCA
GGGATCATGTCATGTCTTCCAGGAAAGCTGCACGCCATGAACGGGGTGAGCGCAAAAAGCGCGATTTTAATAGAGCAACA
TTCCGGACGGATTTTGTTTGCTAAAGATGCTCATACAAAACGGCGCATCGCCAGCATTACGAAAATTATGACCGCCATTT
TGGCGATTGAGTCCGGGAAGCTTGATGATACGGTGACAGTTAGTGCCCGCGCCGTCCGGACGGAGGGGTCATCGATTTAC
TTACGCCAAGGGGAGAAAATGAAACTGCGCGATCTTGTATACGGACTGATGCTTCGTTCCGGCAATGACGCCGCAGTCGC
CATCGCCGAACATGTCGGCGGCAGTGTCGAGGGGTTTGTGTTTTTAATGAACGAAAAGGCGGCCGAGATCGGGATGCGCG
ATACGGAATTTGCCAATCCGCACGGCTTGGACGATGCGGAAAGCCATTATTCGACCGCTTACGACATGGCGTTGTTGATG
CAATATGCAATGAAAAATAAAGAGTTTCGCCGCATTTCCGGAACGAAAGTATACCGCGCCCCTGCCCCGCCGGGCGAAGA
CGGAGCGCGTGTATGGCGGAATAAAAATAAGTTGCTGACAAGCCTTTATGAATATTGCACCGGCGGCAAAACCGGCTATA
CGAAACGGGCACACCGCACACTTGTGACGACAGCGTCGAAAGACGGGATTGATTTGGTCGCTGTGACATTGGACGCTTCG
GATGACTGGAACGATCATATCGCCATGTATGAGTATGGCTTTTCCAACTACCGTATTGCCAAAGTCAGCGGCAAGCGCAT
CGTGAAGAGAATTTCCGACCCGTTTTACAAGGGGCGGTTGCAGGCAGCCCGCGATTTGCGATATCCGGTGACTGAAAAGG
AGATGGGAGAGCTGCGGGTGAAAGTGTATTTGCTGAAGCCGCGCGATGAATGGAAGGAAAACCCAAACCGCGCTCCGACG
GCAGTCGGGAAAGCGGTGCTTTATTTGCGCAAACAGGCGGTCGATGCGGTGCCGCTTTTCTATGAGCCGGAGAAGGACGA
ACGCAGCCGGGGGCTCTTTTCGCAAGCATTTCGATTTTTAGGTGTGAGAAGCGATGGTTAA

Upstream 100 bases:

>100_bases
GGCAGGGGTCTCCCCGCCTGTTTTTTTTTGAGCGGGCAAAAGCCGCCTCCCTTGCCTAGAGAGGTTTCGCTGAAAACATA
ATGCAATTGATAGGGGGCCT

Downstream 100 bases:

>100_bases
CCTCATTTGGGTGATGATGGCGCTTATCGGTATCGTCTTTGCTGCGGCCAATGGCACGATGGAGGAAGTGAATAAGGCGG
TGTTCGAAAGCGCCAAAGAA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MLASVAIFFIIFVRSAHTLVQSMASMSSLFPSRCSLCCAHFSLIGKEEQAIPPCLRKEGDPRRYRMKRWKRLVWLIALTA
GIMSCLPGKLHAMNGVSAKSAILIEQHSGRILFAKDAHTKRRIASITKIMTAILAIESGKLDDTVTVSARAVRTEGSSIY
LRQGEKMKLRDLVYGLMLRSGNDAAVAIAEHVGGSVEGFVFLMNEKAAEIGMRDTEFANPHGLDDAESHYSTAYDMALLM
QYAMKNKEFRRISGTKVYRAPAPPGEDGARVWRNKNKLLTSLYEYCTGGKTGYTKRAHRTLVTTASKDGIDLVAVTLDAS
DDWNDHIAMYEYGFSNYRIAKVSGKRIVKRISDPFYKGRLQAARDLRYPVTEKEMGELRVKVYLLKPRDEWKENPNRAPT
AVGKAVLYLRKQAVDAVPLFYEPEKDERSRGLFSQAFRFLGVRSDG

Sequences:

>Translated_446_residues
MLASVAIFFIIFVRSAHTLVQSMASMSSLFPSRCSLCCAHFSLIGKEEQAIPPCLRKEGDPRRYRMKRWKRLVWLIALTA
GIMSCLPGKLHAMNGVSAKSAILIEQHSGRILFAKDAHTKRRIASITKIMTAILAIESGKLDDTVTVSARAVRTEGSSIY
LRQGEKMKLRDLVYGLMLRSGNDAAVAIAEHVGGSVEGFVFLMNEKAAEIGMRDTEFANPHGLDDAESHYSTAYDMALLM
QYAMKNKEFRRISGTKVYRAPAPPGEDGARVWRNKNKLLTSLYEYCTGGKTGYTKRAHRTLVTTASKDGIDLVAVTLDAS
DDWNDHIAMYEYGFSNYRIAKVSGKRIVKRISDPFYKGRLQAARDLRYPVTEKEMGELRVKVYLLKPRDEWKENPNRAPT
AVGKAVLYLRKQAVDAVPLFYEPEKDERSRGLFSQAFRFLGVRSDG
>Mature_446_residues
MLASVAIFFIIFVRSAHTLVQSMASMSSLFPSRCSLCCAHFSLIGKEEQAIPPCLRKEGDPRRYRMKRWKRLVWLIALTA
GIMSCLPGKLHAMNGVSAKSAILIEQHSGRILFAKDAHTKRRIASITKIMTAILAIESGKLDDTVTVSARAVRTEGSSIY
LRQGEKMKLRDLVYGLMLRSGNDAAVAIAEHVGGSVEGFVFLMNEKAAEIGMRDTEFANPHGLDDAESHYSTAYDMALLM
QYAMKNKEFRRISGTKVYRAPAPPGEDGARVWRNKNKLLTSLYEYCTGGKTGYTKRAHRTLVTTASKDGIDLVAVTLDAS
DDWNDHIAMYEYGFSNYRIAKVSGKRIVKRISDPFYKGRLQAARDLRYPVTEKEMGELRVKVYLLKPRDEWKENPNRAPT
AVGKAVLYLRKQAVDAVPLFYEPEKDERSRGLFSQAFRFLGVRSDG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=263, Percent_Identity=36.5019011406844, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87082022, Length=241, Percent_Identity=34.0248962655602, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1786851, Length=257, Percent_Identity=33.852140077821, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082054, Length=234, Percent_Identity=26.0683760683761, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 50043; Mature: 50043

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLASVAIFFIIFVRSAHTLVQSMASMSSLFPSRCSLCCAHFSLIGKEEQAIPPCLRKEGD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCC
PRRYRMKRWKRLVWLIALTAGIMSCLPGKLHAMNGVSAKSAILIEQHSGRILFAKDAHTK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEEECCCEEEEEECCHHH
RRIASITKIMTAILAIESGKLDDTVTVSARAVRTEGSSIYLRQGEKMKLRDLVYGLMLRS
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCEEEEECCCCCHHHHHHHHHHHCC
GNDAAVAIAEHVGGSVEGFVFLMNEKAAEIGMRDTEFANPHGLDDAESHYSTAYDMALLM
CCCCEEEEHHHCCCCCCEEEEEEECHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QYAMKNKEFRRISGTKVYRAPAPPGEDGARVWRNKNKLLTSLYEYCTGGKTGYTKRAHRT
HHHHCCCHHHCCCCCEEEECCCCCCCCHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHE
LVTTASKDGIDLVAVTLDASDDWNDHIAMYEYGFSNYRIAKVSGKRIVKRISDPFYKGRL
EEEECCCCCCEEEEEEEECCCCCCCCEEEEEECCCCEEEEEECHHHHHHHHCCHHHHHHH
QAARDLRYPVTEKEMGELRVKVYLLKPRDEWKENPNRAPTAVGKAVLYLRKQAVDAVPLF
HHHHHCCCCCCHHHHCCEEEEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEE
YEPEKDERSRGLFSQAFRFLGVRSDG
ECCCCCHHHCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLASVAIFFIIFVRSAHTLVQSMASMSSLFPSRCSLCCAHFSLIGKEEQAIPPCLRKEGD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCC
PRRYRMKRWKRLVWLIALTAGIMSCLPGKLHAMNGVSAKSAILIEQHSGRILFAKDAHTK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEEECCCEEEEEECCHHH
RRIASITKIMTAILAIESGKLDDTVTVSARAVRTEGSSIYLRQGEKMKLRDLVYGLMLRS
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCEEEEECCCCCHHHHHHHHHHHCC
GNDAAVAIAEHVGGSVEGFVFLMNEKAAEIGMRDTEFANPHGLDDAESHYSTAYDMALLM
CCCCEEEEHHHCCCCCCEEEEEEECHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QYAMKNKEFRRISGTKVYRAPAPPGEDGARVWRNKNKLLTSLYEYCTGGKTGYTKRAHRT
HHHHCCCHHHCCCCCEEEECCCCCCCCHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHE
LVTTASKDGIDLVAVTLDASDDWNDHIAMYEYGFSNYRIAKVSGKRIVKRISDPFYKGRL
EEEECCCCCCEEEEEEEECCCCCCCCEEEEEECCCCEEEEEECHHHHHHHHCCHHHHHHH
QAARDLRYPVTEKEMGELRVKVYLLKPRDEWKENPNRAPTAVGKAVLYLRKQAVDAVPLF
HHHHHCCCCCCHHHHCCEEEEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEE
YEPEKDERSRGLFSQAFRFLGVRSDG
ECCCCCHHHCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]