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The map label for this gene is dpnM [H]

Identifier: 138895695

GI number: 138895695

Start: 2177811

End: 2178653

Strand: Reverse

Name: dpnM [H]

Synonym: GTNG_2051

Alternate gene names: 138895695

Gene position: 2178653-2177811 (Counterclockwise)

Preceding gene: 138895696

Following gene: 138895694

Centisome position: 61.36

GC content: 31.55

Gene sequence:

>843_bases
GTGACAAAAAATAAACTAGCACAACCTTTTTTAAAGTGGGCAGGAGGTAAACGTCAGTTACTTCCAGAAATACGTAAATA
TATTCCTAAAAAGATAAACACTTATTACGAACCATTTTTGGGTGCTGGAGCAGTATTATTTGATATTCAACCTAAGAAGG
CTGTAATTAATGACATTAATACTGAACTTATCAATACTTATATCGCTATAAGAGACCACGTTGATGAGCTTATAAATGAT
TTAAAAAAACACAAGAATGAAAAAGAATACTTTTATGCAATTCGTGATTTAGACCGTAAAGAAGAATTTAAGAAGCTTTC
CTTGGTTGAGAGGGCATCACGAATTATTTACTTAAATAAAACGTGTTTTAATGGTCTATTTCGTGTAAACAGTCAAGGTC
ATTTTAATGTTCCTTTTGGTAAATATAAAAACCCTCAAATAGTGAATGAAATTGTTTTAAGAGCTGTTCATAACTATCTA
AATTCTAATGATATAACTATACTAAATGTTGATTTTGAAAAAGCTGTAGAGAACGCCAAAAAAGGAGATTTTATTTATTT
TGACCCTCCTTATGACCCTGTTTCAGATACTTCTTCTTTTACTGGTTACAGTTTATACGGATTTGATAAAGATGACCAGA
TTAGGTTGAGAGATTTGTTCGTTGAACTAGATAAACGAGGGTGCAAGGTCTTATTAAGTAACTCGGCGACTGATTTTATA
AAGGACTTATACAAAGATTTTCATATTGAGGTTGTTTCAGCGAATAGAAACATCAATGCAAATGCTTCAAGGAGAGGAAA
AATTGACGAAGTATTGGTGATGAATTATGAACCTGACAGATAA

Upstream 100 bases:

>100_bases
GATTTATTTGTTTTTGGATTAATGATATGATAAAGATATAGAATCCTTGTTCGGGTATAGTTAATTTAGGATTTGCGGTA
ATTAAGGGAGAGAAGTTACA

Downstream 100 bases:

>100_bases
AGCTTGGGAGAAGTTATTTGAGCGTTATAACATTCTCCAAGAAATTGAGAAAAATGGTTTTTATGAAATTGAAGCAAAGA
CAATAAAAGAAGAGCGAGAG

Product: putative adenine-specific DNA methyltransferase

Products: NA

Alternate protein names: M.DpnIIA; Adenine-specific methyltransferase DpnIIA; M.DpnII 1 [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MTKNKLAQPFLKWAGGKRQLLPEIRKYIPKKINTYYEPFLGAGAVLFDIQPKKAVINDINTELINTYIAIRDHVDELIND
LKKHKNEKEYFYAIRDLDRKEEFKKLSLVERASRIIYLNKTCFNGLFRVNSQGHFNVPFGKYKNPQIVNEIVLRAVHNYL
NSNDITILNVDFEKAVENAKKGDFIYFDPPYDPVSDTSSFTGYSLYGFDKDDQIRLRDLFVELDKRGCKVLLSNSATDFI
KDLYKDFHIEVVSANRNINANASRRGKIDEVLVMNYEPDR

Sequences:

>Translated_280_residues
MTKNKLAQPFLKWAGGKRQLLPEIRKYIPKKINTYYEPFLGAGAVLFDIQPKKAVINDINTELINTYIAIRDHVDELIND
LKKHKNEKEYFYAIRDLDRKEEFKKLSLVERASRIIYLNKTCFNGLFRVNSQGHFNVPFGKYKNPQIVNEIVLRAVHNYL
NSNDITILNVDFEKAVENAKKGDFIYFDPPYDPVSDTSSFTGYSLYGFDKDDQIRLRDLFVELDKRGCKVLLSNSATDFI
KDLYKDFHIEVVSANRNINANASRRGKIDEVLVMNYEPDR
>Mature_279_residues
TKNKLAQPFLKWAGGKRQLLPEIRKYIPKKINTYYEPFLGAGAVLFDIQPKKAVINDINTELINTYIAIRDHVDELINDL
KKHKNEKEYFYAIRDLDRKEEFKKLSLVERASRIIYLNKTCFNGLFRVNSQGHFNVPFGKYKNPQIVNEIVLRAVHNYLN
SNDITILNVDFEKAVENAKKGDFIYFDPPYDPVSDTSSFTGYSLYGFDKDDQIRLRDLFVELDKRGCKVLLSNSATDFIK
DLYKDFHIEVVSANRNINANASRRGKIDEVLVMNYEPDR

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the DpnII endonuclease [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=283, Percent_Identity=33.5689045936396, Blast_Score=152, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32607; Mature: 32475

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNKLAQPFLKWAGGKRQLLPEIRKYIPKKINTYYEPFLGAGAVLFDIQPKKAVINDIN
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
TELINTYIAIRDHVDELINDLKKHKNEKEYFYAIRDLDRKEEFKKLSLVERASRIIYLNK
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEH
TCFNGLFRVNSQGHFNVPFGKYKNPQIVNEIVLRAVHNYLNSNDITILNVDFEKAVENAK
HHHCCCEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHCCCC
KGDFIYFDPPYDPVSDTSSFTGYSLYGFDKDDQIRLRDLFVELDKRGCKVLLSNSATDFI
CCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHH
KDLYKDFHIEVVSANRNINANASRRGKIDEVLVMNYEPDR
HHHHHHHEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
TKNKLAQPFLKWAGGKRQLLPEIRKYIPKKINTYYEPFLGAGAVLFDIQPKKAVINDIN
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
TELINTYIAIRDHVDELINDLKKHKNEKEYFYAIRDLDRKEEFKKLSLVERASRIIYLNK
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEH
TCFNGLFRVNSQGHFNVPFGKYKNPQIVNEIVLRAVHNYLNSNDITILNVDFEKAVENAK
HHHCCCEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHCCCC
KGDFIYFDPPYDPVSDTSSFTGYSLYGFDKDDQIRLRDLFVELDKRGCKVLLSNSATDFI
CCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHH
KDLYKDFHIEVVSANRNINANASRRGKIDEVLVMNYEPDR
HHHHHHHEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2989823; 3019562; 2824782; 9862809 [H]