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The map label for this gene is malE [C]

Identifier: 138895666

GI number: 138895666

Start: 2140975

End: 2142294

Strand: Reverse

Name: malE [C]

Synonym: GTNG_2022

Alternate gene names: 138895666

Gene position: 2142294-2140975 (Counterclockwise)

Preceding gene: 138895667

Following gene: 138895665

Centisome position: 60.34

GC content: 46.74

Gene sequence:

>1320_bases
ATGAAAAAGAAAAAAGCGTTTCAATCATCAGCTATTTTGTTAGCGCTTACGATGGGACTTACGGCCTGCTCCGGCGGTGA
AAATGCTGACGGTGATGGCAATAGCAAAACAGAAGGCAATAAAGAAAGCAAATCCAGCGAAGTGGTTAAGATTGTCTATG
CCCGTGGCCAAGACTCGACGAAAGCAACCGAAAAGTTGATCGAAGCATTTGAAAAAGCACATCCGAACATCGATGTTGAA
CTGCGGGAAATGCCGGCTGACACCGGCAAGCAGCATGATGCTTACGTTACGATGTTAAACGCCCAGTCGTCGGAAATCGA
TGTTATGGACCTTGATGTGATCTGGCCGGCTGAATTCGCCCAAGCAGGCTATACGTTGCCATTGGACCGTTTCATTGAGA
AAGATGGCATCGATTTAAGCAAATACAACCAAGGGGCGTTAGCCGCTGGAAACTTCAACGGTAAGCAATGGGCGATGCCG
AAATTTATTGATACGGGCATGTTGTTTTATCGGACGGATTTAGTGCCTGAAGATAAAGTGCCGAAAACATGGGATGAACT
GTTAAAAACAGCAAAGGAACTGAAAGGGCAAGGCGGTACGAAATTCGGTTATTTAATGCAAGCGAAACAATATGAAGGGC
TTGTTTGTAACGCTGTTGAATTTATTGCTTCCTACGGGGGACAAATCGTTGACAAAGACGGAAACGTTGTCGTCAACAGT
CCGGAAGCCATTAAAGGTTTGAAAAAAATGGTGGAGATCGTTCAATCGGATGTTGTGCCAAGCAACGTGACGACCTTTAT
GGAACCTGAGTCTCACACCGCATTCATCGAAGGACAAGCACCGTTTATTCGCAACTGGCCGTATCAATATGCCTTGGCGA
ACGATCAGGAACAATCAAAAATTGTCGGCAAAGTTAGCGTTGCTCCGCTCCCGGCTGGCGATAAAGGGTCAGCTGCAGCG
CTTGGTGGCTGGATGACGGCAATCAACAAATATTCGAAACACCCGAAAGAAGCATGGGAATTCGTCAAATTTATGACCGG
GCCGGAAGGGCAAAAAATCTCGGCCGTTTATGGCGGTTTAGCCCCGACATTGCCGGAGTTGTACGAGGATGAAGAGGTGT
TGAAAGCCAATCCGTTCTTCGCTGAAAAAGGGTTTGTCAACGCCTTGAATGCAGCAGTGCCGCGTCCAGTGGTGCCAAAC
TATCCGGAAATTTCCGAAATCATCCAAATTAACGTATCGAAGGCATTGGCCGGAGAGCTTACTGTTGAACAAGCGGTAGC
AAACATGGAAAAAGAAATGAAGGCAGCGATGAATAAGTAA

Upstream 100 bases:

>100_bases
CATTTCAGCCTAGGAGGTGATCCGGACCGCGCGAGAGATGTGTTGTTCGGTTTCACTCAAGCTTTATCATACAAAAAACA
AGTAGGGGGATGGATAAGAA

Downstream 100 bases:

>100_bases
AGCGGCTGTGTTGCTCGGCGCGGTGCAGCCCAAATGAGCAAGAAAAAATAACGGAGGAAGCCATTCGCTATGTATTAGCC
TTCAGGCCGCTCTCTATCCG

Product: ABC transporter substrate-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKKKKAFQSSAILLALTMGLTACSGGENADGDGNSKTEGNKESKSSEVVKIVYARGQDSTKATEKLIEAFEKAHPNIDVE
LREMPADTGKQHDAYVTMLNAQSSEIDVMDLDVIWPAEFAQAGYTLPLDRFIEKDGIDLSKYNQGALAAGNFNGKQWAMP
KFIDTGMLFYRTDLVPEDKVPKTWDELLKTAKELKGQGGTKFGYLMQAKQYEGLVCNAVEFIASYGGQIVDKDGNVVVNS
PEAIKGLKKMVEIVQSDVVPSNVTTFMEPESHTAFIEGQAPFIRNWPYQYALANDQEQSKIVGKVSVAPLPAGDKGSAAA
LGGWMTAINKYSKHPKEAWEFVKFMTGPEGQKISAVYGGLAPTLPELYEDEEVLKANPFFAEKGFVNALNAAVPRPVVPN
YPEISEIIQINVSKALAGELTVEQAVANMEKEMKAAMNK

Sequences:

>Translated_439_residues
MKKKKAFQSSAILLALTMGLTACSGGENADGDGNSKTEGNKESKSSEVVKIVYARGQDSTKATEKLIEAFEKAHPNIDVE
LREMPADTGKQHDAYVTMLNAQSSEIDVMDLDVIWPAEFAQAGYTLPLDRFIEKDGIDLSKYNQGALAAGNFNGKQWAMP
KFIDTGMLFYRTDLVPEDKVPKTWDELLKTAKELKGQGGTKFGYLMQAKQYEGLVCNAVEFIASYGGQIVDKDGNVVVNS
PEAIKGLKKMVEIVQSDVVPSNVTTFMEPESHTAFIEGQAPFIRNWPYQYALANDQEQSKIVGKVSVAPLPAGDKGSAAA
LGGWMTAINKYSKHPKEAWEFVKFMTGPEGQKISAVYGGLAPTLPELYEDEEVLKANPFFAEKGFVNALNAAVPRPVVPN
YPEISEIIQINVSKALAGELTVEQAVANMEKEMKAAMNK
>Mature_439_residues
MKKKKAFQSSAILLALTMGLTACSGGENADGDGNSKTEGNKESKSSEVVKIVYARGQDSTKATEKLIEAFEKAHPNIDVE
LREMPADTGKQHDAYVTMLNAQSSEIDVMDLDVIWPAEFAQAGYTLPLDRFIEKDGIDLSKYNQGALAAGNFNGKQWAMP
KFIDTGMLFYRTDLVPEDKVPKTWDELLKTAKELKGQGGTKFGYLMQAKQYEGLVCNAVEFIASYGGQIVDKDGNVVVNS
PEAIKGLKKMVEIVQSDVVPSNVTTFMEPESHTAFIEGQAPFIRNWPYQYALANDQEQSKIVGKVSVAPLPAGDKGSAAA
LGGWMTAINKYSKHPKEAWEFVKFMTGPEGQKISAVYGGLAPTLPELYEDEEVLKANPFFAEKGFVNALNAAVPRPVVPN
YPEISEIIQINVSKALAGELTVEQAVANMEKEMKAAMNK

Specific function: Probably part of a binding-protein-dependent transport system [H]

COG id: COG1653

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 47914; Mature: 47914

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKKAFQSSAILLALTMGLTACSGGENADGDGNSKTEGNKESKSSEVVKIVYARGQDST
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCH
KATEKLIEAFEKAHPNIDVELREMPADTGKQHDAYVTMLNAQSSEIDVMDLDVIWPAEFA
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCEEEEEEEECCHHHH
QAGYTLPLDRFIEKDGIDLSKYNQGALAAGNFNGKQWAMPKFIDTGMLFYRTDLVPEDKV
HCCCCCCHHHHHHHCCCCHHHCCCCCEEECCCCCCCCCCCHHHHCCEEEEEECCCCCCCC
PKTWDELLKTAKELKGQGGTKFGYLMQAKQYEGLVCNAVEFIASYGGQIVDKDGNVVVNS
CHHHHHHHHHHHHHCCCCCCCHHHHEEHHHCCCCHHHHHHHHHHCCCEEEECCCCEEECC
PEAIKGLKKMVEIVQSDVVPSNVTTFMEPESHTAFIEGQAPFIRNWPYQYALANDQEQSK
CHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCEEEEECCCCHHHCCCEEEEECCCCHHHH
IVGKVSVAPLPAGDKGSAAALGGWMTAINKYSKHPKEAWEFVKFMTGPEGQKISAVYGGL
HEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEHHHHCCC
APTLPELYEDEEVLKANPFFAEKGFVNALNAAVPRPVVPNYPEISEIIQINVSKALAGEL
CCCCHHHHCCCHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHEEHHHHHHHHHH
TVEQAVANMEKEMKAAMNK
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKKKAFQSSAILLALTMGLTACSGGENADGDGNSKTEGNKESKSSEVVKIVYARGQDST
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCH
KATEKLIEAFEKAHPNIDVELREMPADTGKQHDAYVTMLNAQSSEIDVMDLDVIWPAEFA
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCEEEEEEEECCHHHH
QAGYTLPLDRFIEKDGIDLSKYNQGALAAGNFNGKQWAMPKFIDTGMLFYRTDLVPEDKV
HCCCCCCHHHHHHHCCCCHHHCCCCCEEECCCCCCCCCCCHHHHCCEEEEEECCCCCCCC
PKTWDELLKTAKELKGQGGTKFGYLMQAKQYEGLVCNAVEFIASYGGQIVDKDGNVVVNS
CHHHHHHHHHHHHHCCCCCCCHHHHEEHHHCCCCHHHHHHHHHHCCCEEEECCCCEEECC
PEAIKGLKKMVEIVQSDVVPSNVTTFMEPESHTAFIEGQAPFIRNWPYQYALANDQEQSK
CHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCEEEEECCCCHHHCCCEEEEECCCCHHHH
IVGKVSVAPLPAGDKGSAAALGGWMTAINKYSKHPKEAWEFVKFMTGPEGQKISAVYGGL
HEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEHHHHCCC
APTLPELYEDEEVLKANPFFAEKGFVNALNAAVPRPVVPNYPEISEIIQINVSKALAGEL
CCCCHHHHCCCHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHEEHHHHHHHHHH
TVEQAVANMEKEMKAAMNK
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]