Click here to switch to the map view.

The map label for this gene is shiA [H]

Identifier: 138895662

GI number: 138895662

Start: 2136432

End: 2137724

Strand: Reverse

Name: shiA [H]

Synonym: GTNG_2018

Alternate gene names: 138895662

Gene position: 2137724-2136432 (Counterclockwise)

Preceding gene: 138895663

Following gene: 138895661

Centisome position: 60.21

GC content: 54.06

Gene sequence:

>1293_bases
ATGACAACGACGATGGATGCGTCAGAGTTAAAACGCCAAAAAAGGCGGGCCGCGATTTCGAGCGTGGTCGGCACGACGAT
TGAATGGTATGATTTCTTCCTTTATGGGACGATGGCTGCGCTAGTGTTTCCACAGCTCTTTTTCCCGCAAAGCGACCCAT
ACGTGGCGCTTATGCAATCGTTCACGACGTTCGCCTTAGGGTTTGTTGCCCGGCCGGTCGGAGCGGCCATTTTTGGCCAT
TTCGGCGATAAAATTGGCCGGAAGGCAACGCTGGTCGCCACTTTGTTGTTGATGGGGCTGGCGACGGCGTTGATCGGGTT
GATGCCGACGTACGAGCAAATTGGCTTATGGGCTCCGCTCTTGGTGACCGTGCTTCGTCTCATTCAAGGAATCGGGGTTG
GCGGAGAATGGGGCGGCGCCATTTTGATTGCGATGGAGTGGGAGGAAGGAAAGCGGCGTGGATTGATGGCTTCGCTGCCG
CAAATGGGCGTGCCGTTTGGGCTGCTCACTTCTTCACTTGTGACGACGCTCATGCTCGCAGTAGGCGGCGACAGCTTTTA
TGCATGGGGATGGCGCGTTCCTTTTCTGCTTAGTTTTCTGTTGATCGCTGTCGGATTATATATCCGACTGAAAGTGCTGG
AGTCGCCGCTATTCCAAGAGGCCCTGGCAAAACAAGAAACGAGCAAGATGCCGGTCGGCGAGGTGCTTGTCAAACATCCG
CGCGAAATTCTTTGGTCTGCGCTCGCACGGGTGATTGAAAACGGATCGTTCTATATTTTTGTCACATTTATCGTCAGCTA
TGGGACGACATTTTTGGAGATGGAAAAAAGCATTTTCGTCAATGCGACAATTGTCGCTGCGATCATCAATGCGCTTGCCA
TTGTGTATTCCGGTCATTTATCTGACCGGTGGGGGCGCCGCCGTGTCTACATGACGGGTACCATTTTGCTTATGCTTTGG
GCGTTCCCGTATTATTGGCTCGTCAACACGAAAAGCGTAGGCCTGATTATGTTGGCGACGATCATCGCCATGGTATTCCA
CGGCATGCTGTATGGCCCGCAGGCGGCGATGATCGCGGAAAACTTCCCGACACGCCTGCGCTATAGCGGCGCTTCGCTCG
GCTACCAGCTGGCCGCGATCATTGCCGGTGGACCAGCGCCGCTCATCAGCACGTGGCTGCTGCACAAATACGGCAGCGCT
ACCGCGATTTCCTTCTATATCGTGGTCATGGGATTGATTTCGCTCGTCGGTGTCAAAATGCTTCAAGACCGAGCCCGCCA
AGCGATTGATTAA

Upstream 100 bases:

>100_bases
AAAGCGTTAACATCGAAGCGAATAATCAACCGAGGTCGCTTTGGCTATTATTCATTTGTTATTTTTGCGTTTTCATATGA
TGAAAGGGGTGCTGGCGTCA

Downstream 100 bases:

>100_bases
TAATCGATCCGAACCCATAGGCGCCTCTGCATTCGTGCAGAGGCGTTTTTGGTGCTGAATCGGTTGTCTTTCATGTAAAA
AACAGAAAAACGTTAGATCC

Product: putative integral membrane transport protein

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTTTMDASELKRQKRRAAISSVVGTTIEWYDFFLYGTMAALVFPQLFFPQSDPYVALMQSFTTFALGFVARPVGAAIFGH
FGDKIGRKATLVATLLLMGLATALIGLMPTYEQIGLWAPLLVTVLRLIQGIGVGGEWGGAILIAMEWEEGKRRGLMASLP
QMGVPFGLLTSSLVTTLMLAVGGDSFYAWGWRVPFLLSFLLIAVGLYIRLKVLESPLFQEALAKQETSKMPVGEVLVKHP
REILWSALARVIENGSFYIFVTFIVSYGTTFLEMEKSIFVNATIVAAIINALAIVYSGHLSDRWGRRRVYMTGTILLMLW
AFPYYWLVNTKSVGLIMLATIIAMVFHGMLYGPQAAMIAENFPTRLRYSGASLGYQLAAIIAGGPAPLISTWLLHKYGSA
TAISFYIVVMGLISLVGVKMLQDRARQAID

Sequences:

>Translated_430_residues
MTTTMDASELKRQKRRAAISSVVGTTIEWYDFFLYGTMAALVFPQLFFPQSDPYVALMQSFTTFALGFVARPVGAAIFGH
FGDKIGRKATLVATLLLMGLATALIGLMPTYEQIGLWAPLLVTVLRLIQGIGVGGEWGGAILIAMEWEEGKRRGLMASLP
QMGVPFGLLTSSLVTTLMLAVGGDSFYAWGWRVPFLLSFLLIAVGLYIRLKVLESPLFQEALAKQETSKMPVGEVLVKHP
REILWSALARVIENGSFYIFVTFIVSYGTTFLEMEKSIFVNATIVAAIINALAIVYSGHLSDRWGRRRVYMTGTILLMLW
AFPYYWLVNTKSVGLIMLATIIAMVFHGMLYGPQAAMIAENFPTRLRYSGASLGYQLAAIIAGGPAPLISTWLLHKYGSA
TAISFYIVVMGLISLVGVKMLQDRARQAID
>Mature_429_residues
TTTMDASELKRQKRRAAISSVVGTTIEWYDFFLYGTMAALVFPQLFFPQSDPYVALMQSFTTFALGFVARPVGAAIFGHF
GDKIGRKATLVATLLLMGLATALIGLMPTYEQIGLWAPLLVTVLRLIQGIGVGGEWGGAILIAMEWEEGKRRGLMASLPQ
MGVPFGLLTSSLVTTLMLAVGGDSFYAWGWRVPFLLSFLLIAVGLYIRLKVLESPLFQEALAKQETSKMPVGEVLVKHPR
EILWSALARVIENGSFYIFVTFIVSYGTTFLEMEKSIFVNATIVAAIINALAIVYSGHLSDRWGRRRVYMTGTILLMLWA
FPYYWLVNTKSVGLIMLATIIAMVFHGMLYGPQAAMIAENFPTRLRYSGASLGYQLAAIIAGGPAPLISTWLLHKYGSAT
AISFYIVVMGLISLVGVKMLQDRARQAID

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=432, Percent_Identity=38.6574074074074, Blast_Score=304, Evalue=8e-84,
Organism=Escherichia coli, GI1789941, Length=437, Percent_Identity=37.2997711670481, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI1790550, Length=438, Percent_Identity=31.7351598173516, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1788942, Length=440, Percent_Identity=29.0909090909091, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI87082404, Length=430, Percent_Identity=23.2558139534884, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87082231, Length=478, Percent_Identity=21.7573221757322, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47291; Mature: 47160

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTMDASELKRQKRRAAISSVVGTTIEWYDFFLYGTMAALVFPQLFFPQSDPYVALMQS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
FTTFALGFVARPVGAAIFGHFGDKIGRKATLVATLLLMGLATALIGLMPTYEQIGLWAPL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHH
LVTVLRLIQGIGVGGEWGGAILIAMEWEEGKRRGLMASLPQMGVPFGLLTSSLVTTLMLA
HHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHH
VGGDSFYAWGWRVPFLLSFLLIAVGLYIRLKVLESPLFQEALAKQETSKMPVGEVLVKHP
HCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHCH
REILWSALARVIENGSFYIFVTFIVSYGTTFLEMEKSIFVNATIVAAIINALAIVYSGHL
HHHHHHHHHHHHHCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SDRWGRRRVYMTGTILLMLWAFPYYWLVNTKSVGLIMLATIIAMVFHGMLYGPQAAMIAE
CCCCCCEEEEEHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
NFPTRLRYSGASLGYQLAAIIAGGPAPLISTWLLHKYGSATAISFYIVVMGLISLVGVKM
CCCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LQDRARQAID
HHHHHHHCCC
>Mature Secondary Structure 
TTTMDASELKRQKRRAAISSVVGTTIEWYDFFLYGTMAALVFPQLFFPQSDPYVALMQS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
FTTFALGFVARPVGAAIFGHFGDKIGRKATLVATLLLMGLATALIGLMPTYEQIGLWAPL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHH
LVTVLRLIQGIGVGGEWGGAILIAMEWEEGKRRGLMASLPQMGVPFGLLTSSLVTTLMLA
HHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHH
VGGDSFYAWGWRVPFLLSFLLIAVGLYIRLKVLESPLFQEALAKQETSKMPVGEVLVKHP
HCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHCH
REILWSALARVIENGSFYIFVTFIVSYGTTFLEMEKSIFVNATIVAAIINALAIVYSGHL
HHHHHHHHHHHHHCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SDRWGRRRVYMTGTILLMLWAFPYYWLVNTKSVGLIMLATIIAMVFHGMLYGPQAAMIAE
CCCCCCEEEEEHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
NFPTRLRYSGASLGYQLAAIIAGGPAPLISTWLLHKYGSATAISFYIVVMGLISLVGVKM
CCCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LQDRARQAID
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]