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The map label for this gene is paaF [C]

Identifier: 138895542

GI number: 138895542

Start: 2018708

End: 2019505

Strand: Reverse

Name: paaF [C]

Synonym: GTNG_1892

Alternate gene names: 138895542

Gene position: 2019505-2018708 (Counterclockwise)

Preceding gene: 138895543

Following gene: 138895541

Centisome position: 56.88

GC content: 52.26

Gene sequence:

>798_bases
ATGGTAGCTGTAGAAACGAAGAAACAATATTTGACGGTCGTTAAAGAAGAGGGGATTGCGGAAATTCACTTGCATATTAA
CAAGTCGAACTCGTATGATTTGGAATTTTACAAGGAGTTCAACGCGGCGATTGATGACATTCGCTTCGATCCGGACATCA
AAGTGGTCATTATCATGAGCGATGTGCCGAAGTTTTTCTCGGCTGGGGCGGACATTAACTTTTTACGTTCGGCCGATCCG
CGGTTCAAAACGCAATTTTGCCTGTTCTGCAACGAGACGCTCGATAAAATCGCCCGCTCGCCGCAAGTGTACATCGCCTG
CTTAGAAGGGCATACAGTCGGCGGGGGCTTGGAGATGGCGTTAGCGTGCGACTTGCGGTTTATGGGCGATGAAGCCGGGA
AAATCGGGTTGCCCGAAGTGACGCTCGGGGTGTTGGCCGGGACGGGAGGAACGCAGCGGCTGGCGCGGTTGGTCGGCTAT
TCGCGTGCGTTGGATATGAACATTACCGGGGAAACGATCACTCCACAAGAAGCGCTGGATATCGGGCTGGTGAACCGGGT
GTTCCCGCAGGCAGAAACGAGAGAGCGGACGCGGGAGTACGCGCGCAAACTTGTCAACAGCGCGACGTATGCGATTTCGA
ACATCAAGCTGTCGATCATGAACGGGAAAGAAATGCCGCTGAACGTGGCGATCCGCTATGAAGGCGAGCTGCAAAACTTG
TTGTTCCGTTCGGAAGACGCCAAAGAAGGATTGAGTGCATTTTTGGAAAAACGCCAACCGAATTGGAAAGGAGTTTAA

Upstream 100 bases:

>100_bases
TTCGGAAAGACAAAGATGGAAATGAACTTTCAAAAACGGGTTATTGGAAAGTTGCCTGCATCACAACATCAAAAAATTGA
TAGGGAAGGAGAATAAGAGA

Downstream 100 bases:

>100_bases
GCGACACTGTTATGAAGCCCTTGAAATGGGAAGCCGGTTCATCCATTTCCAAGGGCTTCCTCATGCATATTTATCAAGCA
GTCCAATTTTTATAGAAATG

Product: putative enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MVAVETKKQYLTVVKEEGIAEIHLHINKSNSYDLEFYKEFNAAIDDIRFDPDIKVVIIMSDVPKFFSAGADINFLRSADP
RFKTQFCLFCNETLDKIARSPQVYIACLEGHTVGGGLEMALACDLRFMGDEAGKIGLPEVTLGVLAGTGGTQRLARLVGY
SRALDMNITGETITPQEALDIGLVNRVFPQAETRERTREYARKLVNSATYAISNIKLSIMNGKEMPLNVAIRYEGELQNL
LFRSEDAKEGLSAFLEKRQPNWKGV

Sequences:

>Translated_265_residues
MVAVETKKQYLTVVKEEGIAEIHLHINKSNSYDLEFYKEFNAAIDDIRFDPDIKVVIIMSDVPKFFSAGADINFLRSADP
RFKTQFCLFCNETLDKIARSPQVYIACLEGHTVGGGLEMALACDLRFMGDEAGKIGLPEVTLGVLAGTGGTQRLARLVGY
SRALDMNITGETITPQEALDIGLVNRVFPQAETRERTREYARKLVNSATYAISNIKLSIMNGKEMPLNVAIRYEGELQNL
LFRSEDAKEGLSAFLEKRQPNWKGV
>Mature_265_residues
MVAVETKKQYLTVVKEEGIAEIHLHINKSNSYDLEFYKEFNAAIDDIRFDPDIKVVIIMSDVPKFFSAGADINFLRSADP
RFKTQFCLFCNETLDKIARSPQVYIACLEGHTVGGGLEMALACDLRFMGDEAGKIGLPEVTLGVLAGTGGTQRLARLVGY
SRALDMNITGETITPQEALDIGLVNRVFPQAETRERTREYARKLVNSATYAISNIKLSIMNGKEMPLNVAIRYEGELQNL
LFRSEDAKEGLSAFLEKRQPNWKGV

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=225, Percent_Identity=32.8888888888889, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI20127408, Length=199, Percent_Identity=33.1658291457286, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI31542718, Length=264, Percent_Identity=30.6818181818182, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI4502327, Length=258, Percent_Identity=32.5581395348837, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI68989263, Length=278, Percent_Identity=29.8561151079137, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI157694516, Length=185, Percent_Identity=30.2702702702703, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI213417737, Length=185, Percent_Identity=30.2702702702703, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI157694520, Length=185, Percent_Identity=30.2702702702703, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI70995211, Length=267, Percent_Identity=25.4681647940075, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI62530384, Length=243, Percent_Identity=25.9259259259259, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI150378541, Length=256, Percent_Identity=27.734375, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI261878539, Length=183, Percent_Identity=31.6939890710383, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI295842266, Length=242, Percent_Identity=24.7933884297521, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=240, Percent_Identity=36.6666666666667, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=36.3984674329502, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1788682, Length=204, Percent_Identity=35.7843137254902, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI87082183, Length=272, Percent_Identity=30.5147058823529, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1788597, Length=255, Percent_Identity=28.6274509803922, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1790281, Length=195, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787660, Length=255, Percent_Identity=25.8823529411765, Blast_Score=81, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17540714, Length=223, Percent_Identity=33.1838565022422, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=30.3149606299213, Blast_Score=126, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI25145438, Length=211, Percent_Identity=33.6492890995261, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17558304, Length=208, Percent_Identity=33.1730769230769, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=208, Percent_Identity=33.6538461538462, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17508951, Length=208, Percent_Identity=33.6538461538462, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17508953, Length=208, Percent_Identity=33.6538461538462, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17536985, Length=271, Percent_Identity=24.3542435424354, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=170, Percent_Identity=32.3529411764706, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=169, Percent_Identity=26.6272189349112, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17534483, Length=208, Percent_Identity=25.9615384615385, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24653139, Length=253, Percent_Identity=34.7826086956522, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI20129971, Length=223, Percent_Identity=33.1838565022422, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24653477, Length=223, Percent_Identity=33.1838565022422, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19921000, Length=184, Percent_Identity=34.7826086956522, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583077, Length=184, Percent_Identity=34.7826086956522, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583079, Length=184, Percent_Identity=34.7826086956522, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922422, Length=264, Percent_Identity=27.6515151515151, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24650670, Length=161, Percent_Identity=37.888198757764, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357171, Length=132, Percent_Identity=35.6060606060606, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29563; Mature: 29563

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAVETKKQYLTVVKEEGIAEIHLHINKSNSYDLEFYKEFNAAIDDIRFDPDIKVVIIMS
CCEECCCHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEC
DVPKFFSAGADINFLRSADPRFKTQFCLFCNETLDKIARSPQVYIACLEGHTVGGGLEMA
CCCHHHHCCCCEEEECCCCCCCCHHEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCEEEE
LACDLRFMGDEAGKIGLPEVTLGVLAGTGGTQRLARLVGYSRALDMNITGETITPQEALD
EEEEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHEEEEECCCCCCCHHHHHH
IGLVNRVFPQAETRERTREYARKLVNSATYAISNIKLSIMNGKEMPLNVAIRYEGELQNL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCEEEEEEECCCHHHH
LFRSEDAKEGLSAFLEKRQPNWKGV
HHCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVAVETKKQYLTVVKEEGIAEIHLHINKSNSYDLEFYKEFNAAIDDIRFDPDIKVVIIMS
CCEECCCHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEC
DVPKFFSAGADINFLRSADPRFKTQFCLFCNETLDKIARSPQVYIACLEGHTVGGGLEMA
CCCHHHHCCCCEEEECCCCCCCCHHEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCEEEE
LACDLRFMGDEAGKIGLPEVTLGVLAGTGGTQRLARLVGYSRALDMNITGETITPQEALD
EEEEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHEEEEECCCCCCCHHHHHH
IGLVNRVFPQAETRERTREYARKLVNSATYAISNIKLSIMNGKEMPLNVAIRYEGELQNL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCEEEEEEECCCHHHH
LFRSEDAKEGLSAFLEKRQPNWKGV
HHCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA