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The map label for this gene is yhaR [H]

Identifier: 138895477

GI number: 138895477

Start: 1941538

End: 1942302

Strand: Reverse

Name: yhaR [H]

Synonym: GTNG_1825

Alternate gene names: 138895477

Gene position: 1942302-1941538 (Counterclockwise)

Preceding gene: 138895478

Following gene: 138895475

Centisome position: 54.71

GC content: 50.85

Gene sequence:

>765_bases
ATGGAAACGGTAGTACTGACGTTTGAGCAAAACAAAGCGATTTTGGAGCTGAACCGCCCGCAGGCGCTCAATGCGATGGA
TATGCAAATGTTAACTGAACTTGTCGATGCGCTGCGGAAAATCAAGGAAAGTGACACTTCGATCGTTGTCATCCGCGGCA
AGGGGAGAGGATTTTCCGCCGGCGGCGACATTAAGACGATGCTTTCAGTTGATGATCCAAGCCAATTCCGTACGGTAATG
AAAACGATTCAAGAGATGATCATGCTATTGTATACGATGCCGAAAATCACCGTTTCGTTCATCCATGGACCTGCTGCTGG
TCTAGGGTTTAGTTTCGCCTTGGCGAGCGATTATCTTATCGCCACGAACGAAGCGCGCCTGGCCATGAACTTTATCGGCG
TTGGTCTTGTCCCTGACGGTGGTGGGCATTTCCTCTTGGCGCAGCGCGTCGGCACGCCAAAAGCGAAACAGATCATTTGG
GAAGGAAAAACGATGAGCGCTGACGAAGCGAAACAGCTTGGCCTCGTCGACCTTGTTGTCGATAGTGAGGAGCAAGCGGA
ACAATGGCTCGCTTCCTTGCAAGAAAAACCGCTCCAAGCCATGATTGCCACAAAATTGTTGTATGCTGAGCAAACAAAGG
CGGACTTGCTTCGCGTCTTGGAGCTTGAAACTGACGCCCAACAGCGCATGCGGCAAACCGAAGACCACCGCGAAGGCATC
CGCGCCTTTTTAGAAAAAAGGAAGCCGGTGTTTCGAGGAAAATAA

Upstream 100 bases:

>100_bases
TTTATAAAAAGAGCGTCCATTTTTTTTCGTGCAGGAAAAGGAGTTTGCCCCTCCGTTAGGGAAATGAAATAAATGAACAA
TCATTCAGAGGGGGAGAGCG

Downstream 100 bases:

>100_bases
GGATGAAACGGTTAACTCCTGCCTGATTTACACTCATGTGCACCTTGGCAGGAGTTTTCTTTTCAGTGCGAATATTCGCT
GTACATGGCACTGTTAATCC

Product: enoyl-CoA hydratase

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
METVVLTFEQNKAILELNRPQALNAMDMQMLTELVDALRKIKESDTSIVVIRGKGRGFSAGGDIKTMLSVDDPSQFRTVM
KTIQEMIMLLYTMPKITVSFIHGPAAGLGFSFALASDYLIATNEARLAMNFIGVGLVPDGGGHFLLAQRVGTPKAKQIIW
EGKTMSADEAKQLGLVDLVVDSEEQAEQWLASLQEKPLQAMIATKLLYAEQTKADLLRVLELETDAQQRMRQTEDHREGI
RAFLEKRKPVFRGK

Sequences:

>Translated_254_residues
METVVLTFEQNKAILELNRPQALNAMDMQMLTELVDALRKIKESDTSIVVIRGKGRGFSAGGDIKTMLSVDDPSQFRTVM
KTIQEMIMLLYTMPKITVSFIHGPAAGLGFSFALASDYLIATNEARLAMNFIGVGLVPDGGGHFLLAQRVGTPKAKQIIW
EGKTMSADEAKQLGLVDLVVDSEEQAEQWLASLQEKPLQAMIATKLLYAEQTKADLLRVLELETDAQQRMRQTEDHREGI
RAFLEKRKPVFRGK
>Mature_254_residues
METVVLTFEQNKAILELNRPQALNAMDMQMLTELVDALRKIKESDTSIVVIRGKGRGFSAGGDIKTMLSVDDPSQFRTVM
KTIQEMIMLLYTMPKITVSFIHGPAAGLGFSFALASDYLIATNEARLAMNFIGVGLVPDGGGHFLLAQRVGTPKAKQIIW
EGKTMSADEAKQLGLVDLVVDSEEQAEQWLASLQEKPLQAMIATKLLYAEQTKADLLRVLELETDAQQRMRQTEDHREGI
RAFLEKRKPVFRGK

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=29.3877551020408, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI45643119, Length=255, Percent_Identity=24.7058823529412, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI260275230, Length=255, Percent_Identity=24.7058823529412, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI260274832, Length=255, Percent_Identity=24.7058823529412, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI4502327, Length=260, Percent_Identity=28.8461538461538, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI31542718, Length=242, Percent_Identity=27.6859504132231, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI157694516, Length=200, Percent_Identity=25, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI213417737, Length=200, Percent_Identity=25, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI68989263, Length=192, Percent_Identity=31.7708333333333, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI37594469, Length=223, Percent_Identity=29.1479820627803, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI37594471, Length=223, Percent_Identity=29.1479820627803, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=27.4436090225564, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI20127408, Length=174, Percent_Identity=28.735632183908, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI157694520, Length=163, Percent_Identity=25.1533742331288, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI62530384, Length=192, Percent_Identity=29.1666666666667, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787660, Length=264, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=32.046332046332, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI87082183, Length=262, Percent_Identity=27.4809160305344, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI221142681, Length=270, Percent_Identity=28.1481481481481, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=28.9156626506024, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790281, Length=191, Percent_Identity=28.7958115183246, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788682, Length=175, Percent_Identity=25.1428571428571, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=29.8804780876494, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI25145438, Length=246, Percent_Identity=31.7073170731707, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=242, Percent_Identity=28.099173553719, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI25141363, Length=191, Percent_Identity=26.1780104712042, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=182, Percent_Identity=29.6703296703297, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI25144157, Length=186, Percent_Identity=31.1827956989247, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144160, Length=186, Percent_Identity=31.1827956989247, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=244, Percent_Identity=25.8196721311475, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=172, Percent_Identity=26.7441860465116, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17536985, Length=222, Percent_Identity=27.4774774774775, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=192, Percent_Identity=28.125, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653139, Length=255, Percent_Identity=29.0196078431373, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI20129971, Length=250, Percent_Identity=28.4, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24653477, Length=250, Percent_Identity=28.4, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922422, Length=245, Percent_Identity=26.530612244898, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24654903, Length=237, Percent_Identity=24.8945147679325, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571730, Length=152, Percent_Identity=30.2631578947368, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571729, Length=152, Percent_Identity=30.2631578947368, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920382, Length=262, Percent_Identity=26.7175572519084, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 28283; Mature: 28283

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METVVLTFEQNKAILELNRPQALNAMDMQMLTELVDALRKIKESDTSIVVIRGKGRGFSA
CCEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
GGDIKTMLSVDDPSQFRTVMKTIQEMIMLLYTMPKITVSFIHGPAAGLGFSFALASDYLI
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHCCHHHHHHCCEEE
ATNEARLAMNFIGVGLVPDGGGHFLLAQRVGTPKAKQIIWEGKTMSADEAKQLGLVDLVV
EECCHHEEEEEEEEEEEECCCCCEEEEECCCCCHHHHEEECCCCCCHHHHHHCCEEEEEE
DSEEQAEQWLASLQEKPLQAMIATKLLYAEQTKADLLRVLELETDAQQRMRQTEDHREGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RAFLEKRKPVFRGK
HHHHHHCCCCCCCC
>Mature Secondary Structure
METVVLTFEQNKAILELNRPQALNAMDMQMLTELVDALRKIKESDTSIVVIRGKGRGFSA
CCEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
GGDIKTMLSVDDPSQFRTVMKTIQEMIMLLYTMPKITVSFIHGPAAGLGFSFALASDYLI
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHCCHHHHHHCCEEE
ATNEARLAMNFIGVGLVPDGGGHFLLAQRVGTPKAKQIIWEGKTMSADEAKQLGLVDLVV
EECCHHEEEEEEEEEEEECCCCCEEEEECCCCCHHHHEEECCCCCCHHHHHHCCEEEEEE
DSEEQAEQWLASLQEKPLQAMIATKLLYAEQTKADLLRVLELETDAQQRMRQTEDHREGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RAFLEKRKPVFRGK
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 9579061 [H]