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The map label for this gene is livJ [H]

Identifier: 138895175

GI number: 138895175

Start: 1596222

End: 1597421

Strand: Direct

Name: livJ [H]

Synonym: GTNG_1513

Alternate gene names: 138895175

Gene position: 1596222-1597421 (Clockwise)

Preceding gene: 138895174

Following gene: 138895176

Centisome position: 44.96

GC content: 48.42

Gene sequence:

>1200_bases
ATGAAAAAAAGAAAATGGCCGATCGCCGCTATGATGACAGCGGTCATGGCGTTGATGCTTGCGGGGTGCAACAGCTCGAC
GACGAACAATCATGCCGACAGCGGCAACAATGACGACGGGAAGAACGTCGTCAATATTGGTTACAGCGGCCCGTTAAGCG
GACCAGCTGCCTACTACGGGGAGCGGACATTAAACGGCGTGAAAATGGCAGCGGAAGAAATCAATAAGAACGGCGGGTTT
GAAGTGAATGGAAAAACTTATCAAATTAATGTTGTATCATTAGACGACAAATATTTGCCGAACGAAACAGCGGCAAATGC
AAAACGGCTTGTCCAAGAACATCATACGCCAATCATCTTCACTCCGCACAGCGGCGGCGTCATGGCGATGCAAGTGTTCA
ACCAAACGGACAATTTCATTATCGCGGCCTATACAAGCGAGCCGAAAATTACAGAAACAGGGAACAAGATGACGATACGT
ATTCCGCCGCGGTATGATGGGTACATTCCAACATTTACCGACTATGTGATAAAGCGATTTGGCAAAAAGCTGGCTGCTTT
GCCGACGGCGACGCAATATGGGAAAGATTGGACGGAACAACTGTTACCATATTGGGAAAAACAAGGCGGAGAAGTTGTTT
ACAACACATCCATTGACTTCACCAAAGACACCGATTTCTTCACGATCATCACGAACGCATTGAAAGAAAAGCCGGACGTC
TTATTTATCGGTGGACCGTCGGAACCGACGGCGAAAGTGGTGAAACAGGCGCGTGAGCTCGGTTTTAAAGGCGGTTTTAT
TGTCATGGACCAAGCGAAGTTAGATGAAATGAAGCGGACCATCGGATCTTACGAGATGCTTGAGGGAGCGATCGGCGTCA
TGCCGCTTGTTGAGTCGAAAGAGGCGGGAGCCCCGCAATTTGCGGAAAACTATCGAGCGAAATACAATGCCGAACCGAGT
TCGGAATCTGCCTATAACTACTTGGCTCTTTACGCCTTTGTCGAAGCGATGAAAGCAGCTGGCACGGTTGATGATCCGGT
TGCTATCCGCGAACATATGAACGAAGGGTTGAAAAACATCCCGGAAGACAAGCAAGTGTATATCGTCCCGAGTGTTGGTG
AGGACGGCGGATTTGAATCGAAAATTACCGTTGGAGCGGTGGAAAAAGGCAAAGTGGTGCCGATTGAACTGAAGAAATAA

Upstream 100 bases:

>100_bases
GAACGCCAAAAGATGCTAATCCATCAACGATGAAAGGAGGTGAGCAACGAGTACTACCTTCATTCATCTGAATATTCGGT
ATAAAAATGGGGGGAGCAGA

Downstream 100 bases:

>100_bases
GCAAACCAACACACGCGGAAAACGGGATGATTTTTCCTGTGTTGCGAGGCGAGCGATGACCTGATCGCCCCATCTTTTCC
AATCGATTTGGAGCAAACAT

Product: branched-chain amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-BP [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MKKRKWPIAAMMTAVMALMLAGCNSSTTNNHADSGNNDDGKNVVNIGYSGPLSGPAAYYGERTLNGVKMAAEEINKNGGF
EVNGKTYQINVVSLDDKYLPNETAANAKRLVQEHHTPIIFTPHSGGVMAMQVFNQTDNFIIAAYTSEPKITETGNKMTIR
IPPRYDGYIPTFTDYVIKRFGKKLAALPTATQYGKDWTEQLLPYWEKQGGEVVYNTSIDFTKDTDFFTIITNALKEKPDV
LFIGGPSEPTAKVVKQARELGFKGGFIVMDQAKLDEMKRTIGSYEMLEGAIGVMPLVESKEAGAPQFAENYRAKYNAEPS
SESAYNYLALYAFVEAMKAAGTVDDPVAIREHMNEGLKNIPEDKQVYIVPSVGEDGGFESKITVGAVEKGKVVPIELKK

Sequences:

>Translated_399_residues
MKKRKWPIAAMMTAVMALMLAGCNSSTTNNHADSGNNDDGKNVVNIGYSGPLSGPAAYYGERTLNGVKMAAEEINKNGGF
EVNGKTYQINVVSLDDKYLPNETAANAKRLVQEHHTPIIFTPHSGGVMAMQVFNQTDNFIIAAYTSEPKITETGNKMTIR
IPPRYDGYIPTFTDYVIKRFGKKLAALPTATQYGKDWTEQLLPYWEKQGGEVVYNTSIDFTKDTDFFTIITNALKEKPDV
LFIGGPSEPTAKVVKQARELGFKGGFIVMDQAKLDEMKRTIGSYEMLEGAIGVMPLVESKEAGAPQFAENYRAKYNAEPS
SESAYNYLALYAFVEAMKAAGTVDDPVAIREHMNEGLKNIPEDKQVYIVPSVGEDGGFESKITVGAVEKGKVVPIELKK
>Mature_399_residues
MKKRKWPIAAMMTAVMALMLAGCNSSTTNNHADSGNNDDGKNVVNIGYSGPLSGPAAYYGERTLNGVKMAAEEINKNGGF
EVNGKTYQINVVSLDDKYLPNETAANAKRLVQEHHTPIIFTPHSGGVMAMQVFNQTDNFIIAAYTSEPKITETGNKMTIR
IPPRYDGYIPTFTDYVIKRFGKKLAALPTATQYGKDWTEQLLPYWEKQGGEVVYNTSIDFTKDTDFFTIITNALKEKPDV
LFIGGPSEPTAKVVKQARELGFKGGFIVMDQAKLDEMKRTIGSYEMLEGAIGVMPLVESKEAGAPQFAENYRAKYNAEPS
SESAYNYLALYAFVEAMKAAGTVDDPVAIREHMNEGLKNIPEDKQVYIVPSVGEDGGFESKITVGAVEKGKVVPIELKK

Specific function: This protein is a component of the leucine, isoleucine, valine, (threonine) transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=251, Percent_Identity=23.9043824701195, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789867, Length=313, Percent_Identity=21.7252396166134, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 43823; Mature: 43823

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRKWPIAAMMTAVMALMLAGCNSSTTNNHADSGNNDDGKNVVNIGYSGPLSGPAAYYG
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHC
ERTLNGVKMAAEEINKNGGFEVNGKTYQINVVSLDDKYLPNETAANAKRLVQEHHTPIIF
CHHHHHHHHHHHHHCCCCCEEECCEEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEE
TPHSGGVMAMQVFNQTDNFIIAAYTSEPKITETGNKMTIRIPPRYDGYIPTFTDYVIKRF
ECCCCCEEEEEEECCCCCEEEEEECCCCCEEECCCEEEEEECCCCCCCCCHHHHHHHHHH
GKKLAALPTATQYGKDWTEQLLPYWEKQGGEVVYNTSIDFTKDTDFFTIITNALKEKPDV
HHHHHHCCCHHHHCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCE
LFIGGPSEPTAKVVKQARELGFKGGFIVMDQAKLDEMKRTIGSYEMLEGAIGVMPLVESK
EEECCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCHHHHHHHHHCCCEEECCC
EAGAPQFAENYRAKYNAEPSSESAYNYLALYAFVEAMKAAGTVDDPVAIREHMNEGLKNI
CCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
PEDKQVYIVPSVGEDGGFESKITVGAVEKGKVVPIELKK
CCCCEEEEEECCCCCCCCCCEEEEEECCCCCEEEEEECC
>Mature Secondary Structure
MKKRKWPIAAMMTAVMALMLAGCNSSTTNNHADSGNNDDGKNVVNIGYSGPLSGPAAYYG
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHC
ERTLNGVKMAAEEINKNGGFEVNGKTYQINVVSLDDKYLPNETAANAKRLVQEHHTPIIF
CHHHHHHHHHHHHHCCCCCEEECCEEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEE
TPHSGGVMAMQVFNQTDNFIIAAYTSEPKITETGNKMTIRIPPRYDGYIPTFTDYVIKRF
ECCCCCEEEEEEECCCCCEEEEEECCCCCEEECCCEEEEEECCCCCCCCCHHHHHHHHHH
GKKLAALPTATQYGKDWTEQLLPYWEKQGGEVVYNTSIDFTKDTDFFTIITNALKEKPDV
HHHHHHCCCHHHHCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCE
LFIGGPSEPTAKVVKQARELGFKGGFIVMDQAKLDEMKRTIGSYEMLEGAIGVMPLVESK
EEECCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCHHHHHHHHHCCCEEECCC
EAGAPQFAENYRAKYNAEPSSESAYNYLALYAFVEAMKAAGTVDDPVAIREHMNEGLKNI
CCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
PEDKQVYIVPSVGEDGGFESKITVGAVEKGKVVPIELKK
CCCCEEEEEECCCCCCCCCCEEEEEECCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA