BacMap

Definition	Geobacillus thermodenitrificans NG80-2 chromosome, complete genome.
Accession	NC_009328
Length	3,550,319

Click here to switch to the map view.

The map label for this gene is yngF [H]

Identifier: 138895113

GI number: 138895113

Start: 1536194

End: 1536976

Strand: Direct

Name: yngF [H]

Synonym: GTNG_1451

Alternate gene names: 138895113

Gene position: 1536194-1536976 (Clockwise)

Preceding gene: 138895112

Following gene: 138895114

Centisome position: 43.27

GC content: 55.68

Gene sequence:

>783_bases
GTGGGAACGTTCGTATCGTTTGACATGCAGGAAAACGGCATTGGTATTGTGACGTTAAACCGCCCCGAGGCGGCCAATGC
GCTTTCAAGAGCGCTTCTCCTAGAGCTTGGCAACTTGTTGCAAGAGATTAAGTTTCAAAAAGACGTGCGTGTCGTCATTT
TGACCGGGGCGGGAGAAAAAGTGTTTTGCGCCGGCGCCGACTTGAAGGAGCGGGCGGGAATGAATGAAACGGAAGTGCGG
CAGGCGGTCGCCTTGATCAGCAAAACAATCAATGAGGTCGAGAAAGTGCCGCAGCCGGTTATCGCCGCCCTAAACGGCTC
GGCGTTTGGCGGCGGGCTCGAGCTCGCTTTAGCATGCGACATTCGTTACGCTGCTGATGACATCCAACTCGGATTGACAG
AAACGTCACTTGGCATCATTCCAGGCGCGGGCGGCACGCAACGGTTGCCGCGTCTTGTCGGCATCGGAAAGGCGAAAGAG
TTGATTTTTGCCGCGAAACGGATCACGGCCAAGGAGGCGGAGCGAATCGGCCTCGTGGAATACGCTGTACCACGCGCCGA
ACTAATGGAGCGGGCGCTTGAATTGGCTCGGCAAATCGCCGATAATGCACCAATTGCCGTCCGCCAAGCGAAACGGGCGG
TGCAAAGCGTGTTTAACGTTGACTTGGAGACCGGATTGGCGATTGAGCAGCTCGCCTATGAGGCGACGATCCCGACAAAA
GACCGACTCGAAGGCTTGCAGGCGTTTAAAGAGAGACGGAAACCGGTATACAAAGGGGAATAA

Upstream 100 bases:

>100_bases
GCAGCCTTGTTTATTCGCGACAAAATTGGTCGTCCGCTCCCAAGCCGCTATTTACAAACGATTTCATCATAAGCAAATTC
ATTTTGGGAGGAAGAGGAAA

Downstream 100 bases:

>100_bases
GACGCTTCGACGCGGCCGGCTGAAAGCCCGGGGAACGCTACAGGCATAGGTTCAAATGGCCGTAAGCGCTTCAATACCAA
GGGGGGAAACCGACGCGGCC

Product: enoyl-CoA hydratase

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MGTFVSFDMQENGIGIVTLNRPEAANALSRALLLELGNLLQEIKFQKDVRVVILTGAGEKVFCAGADLKERAGMNETEVR
QAVALISKTINEVEKVPQPVIAALNGSAFGGGLELALACDIRYAADDIQLGLTETSLGIIPGAGGTQRLPRLVGIGKAKE
LIFAAKRITAKEAERIGLVEYAVPRAELMERALELARQIADNAPIAVRQAKRAVQSVFNVDLETGLAIEQLAYEATIPTK
DRLEGLQAFKERRKPVYKGE

Sequences:

>Translated_260_residues
MGTFVSFDMQENGIGIVTLNRPEAANALSRALLLELGNLLQEIKFQKDVRVVILTGAGEKVFCAGADLKERAGMNETEVR
QAVALISKTINEVEKVPQPVIAALNGSAFGGGLELALACDIRYAADDIQLGLTETSLGIIPGAGGTQRLPRLVGIGKAKE
LIFAAKRITAKEAERIGLVEYAVPRAELMERALELARQIADNAPIAVRQAKRAVQSVFNVDLETGLAIEQLAYEATIPTK
DRLEGLQAFKERRKPVYKGE
>Mature_259_residues
GTFVSFDMQENGIGIVTLNRPEAANALSRALLLELGNLLQEIKFQKDVRVVILTGAGEKVFCAGADLKERAGMNETEVRQ
AVALISKTINEVEKVPQPVIAALNGSAFGGGLELALACDIRYAADDIQLGLTETSLGIIPGAGGTQRLPRLVGIGKAKEL
IFAAKRITAKEAERIGLVEYAVPRAELMERALELARQIADNAPIAVRQAKRAVQSVFNVDLETGLAIEQLAYEATIPTKD
RLEGLQAFKERRKPVYKGE

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=50, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=41.4342629482072, Blast_Score=187, Evalue=8e-48,
Organism=Homo sapiens, GI150378541, Length=254, Percent_Identity=38.9763779527559, Blast_Score=167, Evalue=7e-42,
Organism=Homo sapiens, GI68989263, Length=211, Percent_Identity=35.0710900473934, Blast_Score=127, Evalue=7e-30,
Organism=Homo sapiens, GI70995211, Length=256, Percent_Identity=33.59375, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI20127408, Length=241, Percent_Identity=32.7800829875519, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI31542718, Length=258, Percent_Identity=31.7829457364341, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI157694516, Length=231, Percent_Identity=31.1688311688312, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI213417737, Length=231, Percent_Identity=31.1688311688312, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI261878539, Length=114, Percent_Identity=42.9824561403509, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI157694520, Length=203, Percent_Identity=28.0788177339901, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI37594471, Length=178, Percent_Identity=28.0898876404494, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI37594469, Length=178, Percent_Identity=28.0898876404494, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI25453481, Length=219, Percent_Identity=25.5707762557078, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4757966, Length=219, Percent_Identity=25.5707762557078, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI51538573, Length=219, Percent_Identity=25.5707762557078, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI51538581, Length=219, Percent_Identity=25.5707762557078, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI45643119, Length=210, Percent_Identity=26.1904761904762, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI260275230, Length=210, Percent_Identity=26.1904761904762, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI260274832, Length=210, Percent_Identity=26.1904761904762, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI4757968, Length=219, Percent_Identity=24.6575342465753, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI49355787, Length=219, Percent_Identity=24.6575342465753, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI116235487, Length=180, Percent_Identity=23.8888888888889, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI221136756, Length=218, Percent_Identity=22.9357798165138, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI221136753, Length=218, Percent_Identity=22.9357798165138, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI221307494, Length=180, Percent_Identity=24.4444444444444, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI62530384, Length=205, Percent_Identity=29.2682926829268, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787659, Length=252, Percent_Identity=39.2857142857143, Blast_Score=170, Evalue=7e-44,
Organism=Escherichia coli, GI221142681, Length=259, Percent_Identity=37.0656370656371, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1788597, Length=258, Percent_Identity=30.6201550387597, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1788682, Length=214, Percent_Identity=35.981308411215, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1790281, Length=200, Percent_Identity=36.5, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI87082183, Length=254, Percent_Identity=29.1338582677165, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1787660, Length=255, Percent_Identity=32.156862745098, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17540714, Length=224, Percent_Identity=45.0892857142857, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=41.3934426229508, Blast_Score=190, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=39.8406374501992, Blast_Score=184, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17534483, Length=222, Percent_Identity=31.981981981982, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17558304, Length=222, Percent_Identity=30.1801801801802, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=258, Percent_Identity=27.5193798449612, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17560910, Length=246, Percent_Identity=26.4227642276423, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=207, Percent_Identity=28.0193236714976, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25144276, Length=201, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17508953, Length=201, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17508951, Length=201, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=198, Percent_Identity=29.7979797979798, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=198, Percent_Identity=29.7979797979798, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI25141363, Length=199, Percent_Identity=28.643216080402, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=209, Percent_Identity=25.8373205741627, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=183, Percent_Identity=25.1366120218579, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24653139, Length=258, Percent_Identity=50, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=42.914979757085, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=42.914979757085, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI19920382, Length=212, Percent_Identity=31.1320754716981, Blast_Score=110, Evalue=6e-25,
Organism=Drosophila melanogaster, GI21357171, Length=254, Percent_Identity=27.1653543307087, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=27.3076923076923, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24654903, Length=211, Percent_Identity=24.6445497630332, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24650670, Length=248, Percent_Identity=28.2258064516129, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19921000, Length=180, Percent_Identity=33.8888888888889, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24583077, Length=180, Percent_Identity=33.8888888888889, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24583079, Length=180, Percent_Identity=33.8888888888889, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28571729, Length=141, Percent_Identity=31.9148936170213, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI28571730, Length=141, Percent_Identity=31.9148936170213, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI19921018, Length=201, Percent_Identity=31.3432835820896, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24583165, Length=197, Percent_Identity=28.4263959390863, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 28051; Mature: 27920

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTFVSFDMQENGIGIVTLNRPEAANALSRALLLELGNLLQEIKFQKDVRVVILTGAGEK
CCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCE
VFCAGADLKERAGMNETEVRQAVALISKTINEVEKVPQPVIAALNGSAFGGGLELALACD
EEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEEEE
IRYAADDIQLGLTETSLGIIPGAGGTQRLPRLVGIGKAKELIFAAKRITAKEAERIGLVE
EEECCCCCEECEEECCCCEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHH
YAVPRAELMERALELARQIADNAPIAVRQAKRAVQSVFNVDLETGLAIEQLAYEATIPTK
HHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHCCCCCH
DRLEGLQAFKERRKPVYKGE
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GTFVSFDMQENGIGIVTLNRPEAANALSRALLLELGNLLQEIKFQKDVRVVILTGAGEK
CCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCE
VFCAGADLKERAGMNETEVRQAVALISKTINEVEKVPQPVIAALNGSAFGGGLELALACD
EEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEEEE
IRYAADDIQLGLTETSLGIIPGAGGTQRLPRLVGIGKAKELIFAAKRITAKEAERIGLVE
EEECCCCCEECEEECCCCEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHH
YAVPRAELMERALELARQIADNAPIAVRQAKRAVQSVFNVDLETGLAIEQLAYEATIPTK
HHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHCCCCCH
DRLEGLQAFKERRKPVYKGE
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]