| Definition | Geobacillus thermodenitrificans NG80-2 chromosome, complete genome. |
|---|---|
| Accession | NC_009328 |
| Length | 3,550,319 |
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The map label for this gene is idnO [C]
Identifier: 138894542
GI number: 138894542
Start: 946009
End: 946758
Strand: Direct
Name: idnO [C]
Synonym: GTNG_0872
Alternate gene names: 138894542
Gene position: 946009-946758 (Clockwise)
Preceding gene: 138894541
Following gene: 138894543
Centisome position: 26.65
GC content: 54.27
Gene sequence:
>750_bases ATGGTCGGAGAACGGAAAACGGTAATTGTCACCGGCGGAGCGAACGGAATCGGCAAGGCGATTGCGGAGATGTTCGCAAA ACAGGGAGCGAATGTTGTGATTGCGGACGTTGATGCGAAAAATGGGGAAGCGATCGTTGAGCGGCTGCGAGCCGACGGAC GGAATGCTGTATTTGTGGAGGCAGACGTGCGGAATGAGGCTGACATTAAGCGATTGATAGAGGAAGCTGCCCGCCGCTTT GGCCGCATCGATAATTTAATCAACAATGTGGGCGTCTCGCGTTGGAAATCGCCGTACGAGTTGACCGTGGATGAGTGGGA TGACGTGCTGGCAACGAATTTGCGCAGCGCCTTTTTGGCTTCCCGTGAAGCGGCAAAATATATGCGACGCAACCCTAAAG GTGGGGCGATCGTCAACATCGCCTCGACAAGAGCACTTATGTCCGAGCCGAATTCGGAGGCGTACGCGGCATCCAAAGGC GGCCTCATCGCTCTCACCCATGCGTTGGCCGTGTCGCTTTCCGACGATCGCATTCGTGTCAACTGCATCAGCCCTGGCTG GATTGAAACGGGCGATTACGCGAAATTGCGGGATGTTGACCATCGACAGCACCCGGCCGGCCGCGTCGGCAAGCCAGACG ATATCGCTCGCGCCTGCCTTTATTTATGTGATGAAGAAAACGATTTTATTACCGGAGTAAACCTCGTCATCGATGGAGGG ATGACGAGGAAAATGATTTATATTGAGTAG
Upstream 100 bases:
>100_bases TTCCGTTACTGTTGGTGGTATAATAAAGAGGCCATTTTCGTGTGAAACGAAGAAAGAATATTAGAACATGATAAAGAGGA GAGAAAGGGGAATGGACAAA
Downstream 100 bases:
>100_bases GCGTTGGGTGTTTGCTAAAATAAATAAAATGGAGACGGCTTATTAGCGAAAAGGTGGGGATGGAACGATGAAAATCCGTA AAACGCCGCTAGTTGACGGA
Product: Short chain alcohol dehydrogenase
Products: D-Gluconate; Nicotinamide adenine dinucleotide phosphate [C]
Alternate protein names: NA
Number of amino acids: Translated: 249; Mature: 249
Protein sequence:
>249_residues MVGERKTVIVTGGANGIGKAIAEMFAKQGANVVIADVDAKNGEAIVERLRADGRNAVFVEADVRNEADIKRLIEEAARRF GRIDNLINNVGVSRWKSPYELTVDEWDDVLATNLRSAFLASREAAKYMRRNPKGGAIVNIASTRALMSEPNSEAYAASKG GLIALTHALAVSLSDDRIRVNCISPGWIETGDYAKLRDVDHRQHPAGRVGKPDDIARACLYLCDEENDFITGVNLVIDGG MTRKMIYIE
Sequences:
>Translated_249_residues MVGERKTVIVTGGANGIGKAIAEMFAKQGANVVIADVDAKNGEAIVERLRADGRNAVFVEADVRNEADIKRLIEEAARRF GRIDNLINNVGVSRWKSPYELTVDEWDDVLATNLRSAFLASREAAKYMRRNPKGGAIVNIASTRALMSEPNSEAYAASKG GLIALTHALAVSLSDDRIRVNCISPGWIETGDYAKLRDVDHRQHPAGRVGKPDDIARACLYLCDEENDFITGVNLVIDGG MTRKMIYIE >Mature_249_residues MVGERKTVIVTGGANGIGKAIAEMFAKQGANVVIADVDAKNGEAIVERLRADGRNAVFVEADVRNEADIKRLIEEAARRF GRIDNLINNVGVSRWKSPYELTVDEWDDVLATNLRSAFLASREAAKYMRRNPKGGAIVNIASTRALMSEPNSEAYAASKG GLIALTHALAVSLSDDRIRVNCISPGWIETGDYAKLRDVDHRQHPAGRVGKPDDIARACLYLCDEENDFITGVNLVIDGG MTRKMIYIE
Specific function: Catalyzes A Reversible Reduction Of 5-Ketoglutanate To Form D-Gluconate. Dependent On Nadp, Almost Inactive With NAD. [C]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI59889578, Length=227, Percent_Identity=35.2422907488987, Blast_Score=128, Evalue=4e-30, Organism=Homo sapiens, GI32483357, Length=248, Percent_Identity=30.6451612903226, Blast_Score=123, Evalue=1e-28, Organism=Homo sapiens, GI40254992, Length=237, Percent_Identity=31.2236286919831, Blast_Score=123, Evalue=2e-28, Organism=Homo sapiens, GI15277342, Length=245, Percent_Identity=31.0204081632653, Blast_Score=117, Evalue=1e-26, Organism=Homo sapiens, GI19923817, Length=253, Percent_Identity=30.8300395256917, Blast_Score=115, Evalue=5e-26, Organism=Homo sapiens, GI4758504, Length=250, Percent_Identity=32.8, Blast_Score=112, Evalue=2e-25, Organism=Homo sapiens, GI5031737, Length=246, Percent_Identity=29.2682926829268, Blast_Score=107, Evalue=8e-24, Organism=Homo sapiens, GI126723750, Length=251, Percent_Identity=27.0916334661355, Blast_Score=99, Evalue=3e-21, Organism=Homo sapiens, GI83715985, Length=245, Percent_Identity=31.4285714285714, Blast_Score=99, Evalue=4e-21, Organism=Homo sapiens, GI31542939, Length=191, Percent_Identity=31.413612565445, Blast_Score=99, Evalue=4e-21, Organism=Homo sapiens, GI10190704, Length=245, Percent_Identity=30.6122448979592, Blast_Score=96, Evalue=3e-20, Organism=Homo sapiens, GI109715829, Length=228, Percent_Identity=30.7017543859649, Blast_Score=96, Evalue=4e-20, Organism=Homo sapiens, GI33667109, Length=192, Percent_Identity=31.25, Blast_Score=91, Evalue=8e-19, Organism=Homo sapiens, GI4504505, Length=231, Percent_Identity=30.3030303030303, Blast_Score=88, Evalue=9e-18, Organism=Homo sapiens, GI126723191, Length=179, Percent_Identity=30.7262569832402, Blast_Score=84, Evalue=1e-16, Organism=Homo sapiens, GI224922801, Length=172, Percent_Identity=29.6511627906977, Blast_Score=83, Evalue=2e-16, Organism=Homo sapiens, GI7705925, Length=241, Percent_Identity=26.9709543568465, Blast_Score=83, Evalue=3e-16, Organism=Homo sapiens, GI66933014, Length=246, Percent_Identity=28.4552845528455, Blast_Score=82, Evalue=4e-16, Organism=Homo sapiens, GI40254986, Length=241, Percent_Identity=30.2904564315353, Blast_Score=79, Evalue=3e-15, Organism=Homo sapiens, GI20149619, Length=192, Percent_Identity=30.7291666666667, Blast_Score=78, Evalue=7e-15, Organism=Homo sapiens, GI4503301, Length=226, Percent_Identity=25.2212389380531, Blast_Score=70, Evalue=1e-12, Organism=Homo sapiens, GI4557649, Length=186, Percent_Identity=30.1075268817204, Blast_Score=69, Evalue=3e-12, Organism=Homo sapiens, GI209862901, Length=195, Percent_Identity=28.2051282051282, Blast_Score=66, Evalue=3e-11, Organism=Homo sapiens, GI19923983, Length=195, Percent_Identity=28.2051282051282, Blast_Score=66, Evalue=3e-11, Organism=Escherichia coli, GI1790717, Length=242, Percent_Identity=33.4710743801653, Blast_Score=143, Evalue=1e-35, Organism=Escherichia coli, GI1787335, Length=243, Percent_Identity=35.8024691358025, Blast_Score=142, Evalue=3e-35, Organism=Escherichia coli, GI1789208, Length=243, Percent_Identity=32.5102880658436, Blast_Score=139, Evalue=2e-34, Organism=Escherichia coli, GI87082160, Length=243, Percent_Identity=34.156378600823, Blast_Score=127, Evalue=5e-31, Organism=Escherichia coli, GI1788459, Length=242, Percent_Identity=32.2314049586777, Blast_Score=125, Evalue=3e-30, Organism=Escherichia coli, GI87082100, Length=249, Percent_Identity=29.718875502008, Blast_Score=120, Evalue=8e-29, Organism=Escherichia coli, GI1787905, Length=245, Percent_Identity=32.6530612244898, Blast_Score=114, Evalue=7e-27, Organism=Escherichia coli, GI2367175, Length=242, Percent_Identity=30.5785123966942, Blast_Score=108, Evalue=3e-25, Organism=Escherichia coli, GI1789378, Length=236, Percent_Identity=29.6610169491525, Blast_Score=102, Evalue=2e-23, Organism=Escherichia coli, GI2367365, Length=239, Percent_Identity=32.2175732217573, Blast_Score=101, Evalue=4e-23, Organism=Escherichia coli, GI1786812, Length=251, Percent_Identity=28.2868525896414, Blast_Score=94, Evalue=7e-21, Organism=Escherichia coli, GI1789057, Length=183, Percent_Identity=25.1366120218579, Blast_Score=73, Evalue=2e-14, Organism=Escherichia coli, GI1787526, Length=234, Percent_Identity=24.7863247863248, Blast_Score=71, Evalue=6e-14, Organism=Escherichia coli, GI1787820, Length=192, Percent_Identity=27.6041666666667, Blast_Score=71, Evalue=8e-14, Organism=Caenorhabditis elegans, GI17561402, Length=253, Percent_Identity=33.596837944664, Blast_Score=132, Evalue=2e-31, Organism=Caenorhabditis elegans, GI25147288, Length=239, Percent_Identity=32.6359832635983, Blast_Score=122, Evalue=2e-28, Organism=Caenorhabditis elegans, GI17555706, Length=240, Percent_Identity=32.0833333333333, Blast_Score=122, Evalue=2e-28, Organism=Caenorhabditis elegans, GI17559104, Length=262, Percent_Identity=31.6793893129771, Blast_Score=118, Evalue=4e-27, Organism=Caenorhabditis elegans, GI17560220, Length=262, Percent_Identity=29.3893129770992, Blast_Score=114, Evalue=5e-26, Organism=Caenorhabditis elegans, GI17560676, Length=244, Percent_Identity=31.1475409836066, Blast_Score=112, Evalue=2e-25, Organism=Caenorhabditis elegans, GI17562904, Length=260, Percent_Identity=32.6923076923077, Blast_Score=110, Evalue=6e-25, Organism=Caenorhabditis elegans, GI17562908, Length=259, Percent_Identity=30.1158301158301, Blast_Score=108, Evalue=2e-24, Organism=Caenorhabditis elegans, GI17562906, Length=262, Percent_Identity=29.0076335877863, Blast_Score=107, Evalue=5e-24, Organism=Caenorhabditis elegans, GI17563726, Length=257, Percent_Identity=32.295719844358, Blast_Score=107, Evalue=5e-24, Organism=Caenorhabditis elegans, GI17562910, Length=261, Percent_Identity=32.5670498084291, Blast_Score=107, Evalue=7e-24, Organism=Caenorhabditis elegans, GI72000259, Length=260, Percent_Identity=31.5384615384615, Blast_Score=106, Evalue=1e-23, Organism=Caenorhabditis elegans, GI17551412, Length=261, Percent_Identity=28.3524904214559, Blast_Score=106, Evalue=1e-23, Organism=Caenorhabditis elegans, GI193204405, Length=262, Percent_Identity=27.4809160305344, Blast_Score=104, Evalue=4e-23, Organism=Caenorhabditis elegans, GI17560332, Length=260, Percent_Identity=29.6153846153846, Blast_Score=103, Evalue=1e-22, Organism=Caenorhabditis elegans, GI17562990, Length=264, Percent_Identity=30.3030303030303, Blast_Score=102, Evalue=2e-22, Organism=Caenorhabditis elegans, GI71994600, Length=246, Percent_Identity=26.0162601626016, Blast_Score=102, Evalue=3e-22, Organism=Caenorhabditis elegans, GI71994604, Length=245, Percent_Identity=27.3469387755102, Blast_Score=101, Evalue=3e-22, Organism=Caenorhabditis elegans, GI17538480, Length=260, Percent_Identity=27.6923076923077, Blast_Score=101, Evalue=4e-22, Organism=Caenorhabditis elegans, GI17531453, Length=262, Percent_Identity=29.0076335877863, Blast_Score=100, Evalue=1e-21, Organism=Caenorhabditis elegans, GI17538486, Length=258, Percent_Identity=28.6821705426357, Blast_Score=98, Evalue=3e-21, Organism=Caenorhabditis elegans, GI17560150, Length=269, Percent_Identity=28.2527881040892, Blast_Score=96, Evalue=2e-20, Organism=Caenorhabditis elegans, GI17544670, Length=271, Percent_Identity=28.0442804428044, Blast_Score=94, Evalue=5e-20, Organism=Caenorhabditis elegans, GI17538182, Length=249, Percent_Identity=26.9076305220884, Blast_Score=94, Evalue=5e-20, Organism=Caenorhabditis elegans, GI17536651, Length=249, Percent_Identity=28.5140562248996, Blast_Score=92, Evalue=4e-19, Organism=Caenorhabditis elegans, GI115534694, Length=248, Percent_Identity=29.0322580645161, Blast_Score=89, Evalue=2e-18, Organism=Caenorhabditis elegans, GI17536025, Length=246, Percent_Identity=27.6422764227642, Blast_Score=87, Evalue=7e-18, Organism=Caenorhabditis elegans, GI17565030, Length=260, Percent_Identity=29.6153846153846, Blast_Score=87, Evalue=9e-18, Organism=Caenorhabditis elegans, GI17568967, Length=189, Percent_Identity=30.6878306878307, Blast_Score=78, Evalue=4e-15, Organism=Caenorhabditis elegans, GI17564282, Length=194, Percent_Identity=30.9278350515464, Blast_Score=77, Evalue=6e-15, Organism=Caenorhabditis elegans, GI71982365, Length=191, Percent_Identity=29.8429319371728, Blast_Score=77, Evalue=6e-15, Organism=Caenorhabditis elegans, GI17508651, Length=255, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=4e-14, Organism=Caenorhabditis elegans, GI17508895, Length=192, Percent_Identity=21.875, Blast_Score=72, Evalue=3e-13, Organism=Caenorhabditis elegans, GI193203115, Length=190, Percent_Identity=25.7894736842105, Blast_Score=71, Evalue=7e-13, Organism=Caenorhabditis elegans, GI17561272, Length=236, Percent_Identity=26.271186440678, Blast_Score=70, Evalue=8e-13, Organism=Caenorhabditis elegans, GI193203117, Length=190, Percent_Identity=25.7894736842105, Blast_Score=70, Evalue=9e-13, Organism=Caenorhabditis elegans, GI17567345, Length=170, Percent_Identity=28.2352941176471, Blast_Score=64, Evalue=8e-11, Organism=Saccharomyces cerevisiae, GI6322861, Length=189, Percent_Identity=34.3915343915344, Blast_Score=96, Evalue=5e-21, Organism=Saccharomyces cerevisiae, GI6324126, Length=253, Percent_Identity=27.2727272727273, Blast_Score=85, Evalue=1e-17, Organism=Saccharomyces cerevisiae, GI6323882, Length=192, Percent_Identity=30.7291666666667, Blast_Score=67, Evalue=3e-12, Organism=Drosophila melanogaster, GI23397609, Length=245, Percent_Identity=33.0612244897959, Blast_Score=133, Evalue=1e-31, Organism=Drosophila melanogaster, GI24639444, Length=242, Percent_Identity=30.5785123966942, Blast_Score=124, Evalue=5e-29, Organism=Drosophila melanogaster, GI24644339, Length=246, Percent_Identity=30.8943089430894, Blast_Score=122, Evalue=2e-28, Organism=Drosophila melanogaster, GI21355319, Length=248, Percent_Identity=32.6612903225806, Blast_Score=119, Evalue=2e-27, Organism=Drosophila melanogaster, GI21357041, Length=247, Percent_Identity=30.7692307692308, Blast_Score=116, Evalue=1e-26, Organism=Drosophila melanogaster, GI28571526, Length=249, Percent_Identity=30.5220883534137, Blast_Score=114, Evalue=7e-26, Organism=Drosophila melanogaster, GI17737361, Length=249, Percent_Identity=31.7269076305221, Blast_Score=108, Evalue=4e-24, Organism=Drosophila melanogaster, GI24644337, Length=246, Percent_Identity=30.0813008130081, Blast_Score=106, Evalue=1e-23, Organism=Drosophila melanogaster, GI18859837, Length=230, Percent_Identity=28.2608695652174, Blast_Score=89, Evalue=2e-18, Organism=Drosophila melanogaster, GI24643142, Length=244, Percent_Identity=26.6393442622951, Blast_Score=84, Evalue=8e-17, Organism=Drosophila melanogaster, GI21357673, Length=190, Percent_Identity=27.8947368421053, Blast_Score=73, Evalue=2e-13, Organism=Drosophila melanogaster, GI24584752, Length=184, Percent_Identity=32.0652173913043, Blast_Score=72, Evalue=3e-13, Organism=Drosophila melanogaster, GI21355631, Length=198, Percent_Identity=28.7878787878788, Blast_Score=72, Evalue=3e-13, Organism=Drosophila melanogaster, GI28571387, Length=232, Percent_Identity=26.7241379310345, Blast_Score=71, Evalue=6e-13, Organism=Drosophila melanogaster, GI281364892, Length=189, Percent_Identity=28.042328042328, Blast_Score=71, Evalue=8e-13, Organism=Drosophila melanogaster, GI116007236, Length=224, Percent_Identity=25.8928571428571, Blast_Score=68, Evalue=5e-12, Organism=Drosophila melanogaster, GI24665243, Length=197, Percent_Identity=27.9187817258883, Blast_Score=68, Evalue=7e-12, Organism=Drosophila melanogaster, GI221331218, Length=197, Percent_Identity=27.9187817258883, Blast_Score=67, Evalue=7e-12, Organism=Drosophila melanogaster, GI21358495, Length=189, Percent_Identity=25.3968253968254, Blast_Score=67, Evalue=8e-12, Organism=Drosophila melanogaster, GI24649181, Length=203, Percent_Identity=25.615763546798, Blast_Score=66, Evalue=2e-11, Organism=Drosophila melanogaster, GI28574402, Length=190, Percent_Identity=28.9473684210526, Blast_Score=66, Evalue=3e-11, Organism=Drosophila melanogaster, GI24584744, Length=190, Percent_Identity=28.9473684210526, Blast_Score=65, Evalue=3e-11, Organism=Drosophila melanogaster, GI24584748, Length=184, Percent_Identity=28.2608695652174, Blast_Score=65, Evalue=5e-11, Organism=Drosophila melanogaster, GI24581726, Length=196, Percent_Identity=27.5510204081633, Blast_Score=65, Evalue=6e-11, Organism=Drosophila melanogaster, GI161076933, Length=184, Percent_Identity=28.2608695652174, Blast_Score=65, Evalue=6e-11, Organism=Drosophila melanogaster, GI24665247, Length=198, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=6e-11, Organism=Drosophila melanogaster, GI221331216, Length=198, Percent_Identity=27.7777777777778, Blast_Score=64, Evalue=7e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.69 [C]
Molecular weight: Translated: 27202; Mature: 27202
Theoretical pI: Translated: 6.30; Mature: 6.30
Prosite motif: PS00061 ADH_SHORT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 3.6 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 3.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MVGERKTVIVTGGANGIGKAIAEMFAKQGANVVIADVDAKNGEAIVERLRADGRNAVFVE CCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEE ADVRNEADIKRLIEEAARRFGRIDNLINNVGVSRWKSPYELTVDEWDDVLATNLRSAFLA ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEHHHHHHHHHHHHHHHHHH SREAAKYMRRNPKGGAIVNIASTRALMSEPNSEAYAASKGGLIALTHALAVSLSDDRIRV HHHHHHHHHCCCCCCEEEEEHHHHHHHCCCCCCCEECCCCCEEEEEEEHEEEECCCEEEE NCISPGWIETGDYAKLRDVDHRQHPAGRVGKPDDIARACLYLCDEENDFITGVNLVIDGG EEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEECC MTRKMIYIE CCEEEEEEC >Mature Secondary Structure MVGERKTVIVTGGANGIGKAIAEMFAKQGANVVIADVDAKNGEAIVERLRADGRNAVFVE CCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEE ADVRNEADIKRLIEEAARRFGRIDNLINNVGVSRWKSPYELTVDEWDDVLATNLRSAFLA ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEHHHHHHHHHHHHHHHHHH SREAAKYMRRNPKGGAIVNIASTRALMSEPNSEAYAASKGGLIALTHALAVSLSDDRIRV HHHHHHHHHCCCCCCEEEEEHHHHHHHCCCCCCCEECCCCCEEEEEEEHEEEECCCEEEE NCISPGWIETGDYAKLRDVDHRQHPAGRVGKPDDIARACLYLCDEENDFITGVNLVIDGG EEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEECC MTRKMIYIE CCEEEEEEC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NAD+; NADH; NADP+; NADPH [C]
Metal ions: Mn2+ [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 20 {D-gluconate}} 0.006 {NADPH}} 0.9 {5-keto-D-gluconate}} 0.02 {NADP+}} [C]
Substrates: 5-Dehydro-D-gluconate; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]
Specific reaction: 5-Dehydro-D-gluconate + H+ + Nicotinamide adenine dinucleotide phosphate - reduced <==> D-Gluconate + Nicotinamide adenine dinucleotide phosphate [C]
General reaction: Redox reaction [C]
Inhibitor: Cd2+; Cs2+; Cu2+; Divalent metal ions; Hg2+; Ni2+; p-Chloromercuribenzoate; Sn2+; Sulfhydryl reagents [C]
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 8550425; 10360571 [H]