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The map label for this gene is clpE [H]

Identifier: 138894526

GI number: 138894526

Start: 931807

End: 933942

Strand: Reverse

Name: clpE [H]

Synonym: GTNG_0856

Alternate gene names: 138894526

Gene position: 933942-931807 (Counterclockwise)

Preceding gene: 138894538

Following gene: 138894520

Centisome position: 26.31

GC content: 51.83

Gene sequence:

>2136_bases
ATGCGTTGTCAAGCATGCCAACAACGGGAAGCAACAGTGTTTGTCAACTTACAATGGAACGGTGAGAAAAAGCAACTTCA
TCTTTGCCATACATGCTATGAAAAGCAAAAACAACAATTGTCGATTCCGATAAACTTCGGCTTTTCGCCGTTTTCATTCG
ATGACTGGTTCACCGACCACTTTGCGACGGCCAACGCTCAAGCAGTCGGACCAGAAACAGCAGCCAAACGTCCGCAGCGC
CACGGCGGTGGATTTTTGGATCAATTCGGCCGCAACTTGACACAAATGGCTAAGGCGGGCTTGATTGACCCAGTCATCGG
CCGCGACAAAGAAATCGCACGTGTCATTGAAATTTTAAACCGCCGCAACAAAAACAACCCGGTTTTGATCGGAGAGCCGG
GCGTCGGAAAAACAGCCATCGTCGAAGGGCTTGCTCTGAAGATTGGCGAAGGGCAAGTGCCAGAAAAACTGTTGAACAAA
GAAGTGTACTTGCTTGATGTTGCATCGCTCGTCGCCAACACCGGCATCCGCGGTCAATTTGAAGAGCGGATGAAACGGCT
CATCACCGAACTGCAAGAGCGGAAAAACATCATCTTGTTCATCGACGAAATTCATCTGCTCGTCGGTGCCGGCGCTGCCG
AAGGGTCGATGGACGCCGGCAACATTTTGAAGCCGGCGCTCGCCCGTGGTGAGCTGCAAGTCGTCGGGGCGACAACATTG
AAAGAATACCGGCAAATTGAAAAAGACGCCGCCCTTGAGCGCCGTTTCCAACCGGTCATCGTCCACGAGCCGACCGTTGA
CGAAGCAATTGCCATCTTAAGAGGCATCCAGCCAAAATACGAACAATTCCATCATGTCCGCTATACGGATGAAGCGATCG
AAGCGTGCGTCAAACTTGCGCACCGCTATATCCAAGACCGCTTCCTCCCGGATAAGGCGATCGATTTGCTTGATGAAGCC
GGCTCGAAAGCGAACTTGCGTCTCGGACCGACCGATGAGAAACAATTGCAAGAGCGGTTGATGCAAATCGCGAAAGAAAA
GGATCAAGCAGCTAAAGAAGAAAACTATGAACTGGCGGCAAAACTGCGCGATGAAGAGCTGAAGCTTGAGAAACAACTCG
AACAAGGCGTCACCCAAGAGCACCCTGTTGTGGATGTCGCCGACATCGAGCGGATCATCGCTGACAAAACAGGCATCCCG
GTCGGCAAGCTGCAAGCTGATGAAAAAGAAAAAATGAAACATCTTGAAGACAATTTGGCGAAAAAAGTGATCGGCCAAGC
AGAAGCAGTGAAAAAAGTCGCCAAAGCGATTCGTCGCAGCCGCGCCGGCTTGAAAGCGAAACACCGCCCAGTCGGTTCGT
TCTTGTTCGTCGGTCCGACCGGCGTCGGGAAAACGGAGCTTGCCAAAACGCTCGCCGAGGAGCTGTTCGGCACAAAAGAT
AGCATGATACGCCTTGATATGAGTGAATACATGGAAAAACATTCGGTCTCAAAACTGATCGGCTCGCCGCCAGGCTATGT
CGGCTTTGAAGAAGCCGGCCAGCTGACGGAGAAAGTGCGCCGCAATCCATACAGCATCATCTTGCTTGACGAGATTGAAA
AAGCGCACCCGGATGTTCAGCACATCTTCCTGCAAATTTTAGAAGACGGCCGCTTGACCGACAGTCAAGGCCGCACCGTC
AGCTTCAAAGACACAGTCATCATCGCAACAAGCAACGCTGGTGTAACCGACAAAAAAATCACCGTCGGCTTTGAAAAACA
AAGCGGTGGCGCTTCAAGCATTCTCGACTCTCTGAGCGCCTACTTCAAGCCGGAATTCTTAAACCGCTTCGACGCCATTA
TCGAGTTCAAGCCGCTTGAAAAAGCGCATTTGCTTCAAATTGTCGACTTGATGCTCGATGACGTCAAAACAGCGATGCGC
GAACAAGGCATCGAACTCAAAGTGACCGAAGCGGCGAAAGAAAAACTGGCCGAACTCGGCTATCATCCAGCCTTCGGCGC
CCGTCCGCTCCGCCGCGTCATTCAAGAACATGTCGAAGACAACATCGCTGACTGCCTGCTTGACGCGAACCAACCGGTGC
ACACGATCCGCGTCGACGTCAACGACGGCGCCATTGTAGCGCAAATCGCATCATAA

Upstream 100 bases:

>100_bases
TTTTGTAAAAAATACTTGAAAGTCAAAAAAGGTCAAAGTATAATAAAATCAGAAAGTCAAAGAAAGTCAAAGTCAAACCG
TAAAAAAGGAGGTATGCGTT

Downstream 100 bases:

>100_bases
CCATCTTGTGAACACCGTCTCACCTCGTTTTCAAGGTGAGACGGTTTTTTGCATATTACATCTATAAATATTGTAGTCAA
CTACCTCTTCTTCACTCCTA

Product: ATP-dependent Clp protease-like protein

Products: NA

Alternate protein names: ATPase ClpE; Heat shock protein HSP1 [H]

Number of amino acids: Translated: 711; Mature: 711

Protein sequence:

>711_residues
MRCQACQQREATVFVNLQWNGEKKQLHLCHTCYEKQKQQLSIPINFGFSPFSFDDWFTDHFATANAQAVGPETAAKRPQR
HGGGFLDQFGRNLTQMAKAGLIDPVIGRDKEIARVIEILNRRNKNNPVLIGEPGVGKTAIVEGLALKIGEGQVPEKLLNK
EVYLLDVASLVANTGIRGQFEERMKRLITELQERKNIILFIDEIHLLVGAGAAEGSMDAGNILKPALARGELQVVGATTL
KEYRQIEKDAALERRFQPVIVHEPTVDEAIAILRGIQPKYEQFHHVRYTDEAIEACVKLAHRYIQDRFLPDKAIDLLDEA
GSKANLRLGPTDEKQLQERLMQIAKEKDQAAKEENYELAAKLRDEELKLEKQLEQGVTQEHPVVDVADIERIIADKTGIP
VGKLQADEKEKMKHLEDNLAKKVIGQAEAVKKVAKAIRRSRAGLKAKHRPVGSFLFVGPTGVGKTELAKTLAEELFGTKD
SMIRLDMSEYMEKHSVSKLIGSPPGYVGFEEAGQLTEKVRRNPYSIILLDEIEKAHPDVQHIFLQILEDGRLTDSQGRTV
SFKDTVIIATSNAGVTDKKITVGFEKQSGGASSILDSLSAYFKPEFLNRFDAIIEFKPLEKAHLLQIVDLMLDDVKTAMR
EQGIELKVTEAAKEKLAELGYHPAFGARPLRRVIQEHVEDNIADCLLDANQPVHTIRVDVNDGAIVAQIAS

Sequences:

>Translated_711_residues
MRCQACQQREATVFVNLQWNGEKKQLHLCHTCYEKQKQQLSIPINFGFSPFSFDDWFTDHFATANAQAVGPETAAKRPQR
HGGGFLDQFGRNLTQMAKAGLIDPVIGRDKEIARVIEILNRRNKNNPVLIGEPGVGKTAIVEGLALKIGEGQVPEKLLNK
EVYLLDVASLVANTGIRGQFEERMKRLITELQERKNIILFIDEIHLLVGAGAAEGSMDAGNILKPALARGELQVVGATTL
KEYRQIEKDAALERRFQPVIVHEPTVDEAIAILRGIQPKYEQFHHVRYTDEAIEACVKLAHRYIQDRFLPDKAIDLLDEA
GSKANLRLGPTDEKQLQERLMQIAKEKDQAAKEENYELAAKLRDEELKLEKQLEQGVTQEHPVVDVADIERIIADKTGIP
VGKLQADEKEKMKHLEDNLAKKVIGQAEAVKKVAKAIRRSRAGLKAKHRPVGSFLFVGPTGVGKTELAKTLAEELFGTKD
SMIRLDMSEYMEKHSVSKLIGSPPGYVGFEEAGQLTEKVRRNPYSIILLDEIEKAHPDVQHIFLQILEDGRLTDSQGRTV
SFKDTVIIATSNAGVTDKKITVGFEKQSGGASSILDSLSAYFKPEFLNRFDAIIEFKPLEKAHLLQIVDLMLDDVKTAMR
EQGIELKVTEAAKEKLAELGYHPAFGARPLRRVIQEHVEDNIADCLLDANQPVHTIRVDVNDGAIVAQIAS
>Mature_711_residues
MRCQACQQREATVFVNLQWNGEKKQLHLCHTCYEKQKQQLSIPINFGFSPFSFDDWFTDHFATANAQAVGPETAAKRPQR
HGGGFLDQFGRNLTQMAKAGLIDPVIGRDKEIARVIEILNRRNKNNPVLIGEPGVGKTAIVEGLALKIGEGQVPEKLLNK
EVYLLDVASLVANTGIRGQFEERMKRLITELQERKNIILFIDEIHLLVGAGAAEGSMDAGNILKPALARGELQVVGATTL
KEYRQIEKDAALERRFQPVIVHEPTVDEAIAILRGIQPKYEQFHHVRYTDEAIEACVKLAHRYIQDRFLPDKAIDLLDEA
GSKANLRLGPTDEKQLQERLMQIAKEKDQAAKEENYELAAKLRDEELKLEKQLEQGVTQEHPVVDVADIERIIADKTGIP
VGKLQADEKEKMKHLEDNLAKKVIGQAEAVKKVAKAIRRSRAGLKAKHRPVGSFLFVGPTGVGKTELAKTLAEELFGTKD
SMIRLDMSEYMEKHSVSKLIGSPPGYVGFEEAGQLTEKVRRNPYSIILLDEIEKAHPDVQHIFLQILEDGRLTDSQGRTV
SFKDTVIIATSNAGVTDKKITVGFEKQSGGASSILDSLSAYFKPEFLNRFDAIIEFKPLEKAHLLQIVDLMLDDVKTAMR
EQGIELKVTEAAKEKLAELGYHPAFGARPLRRVIQEHVEDNIADCLLDANQPVHTIRVDVNDGAIVAQIAS

Specific function: ATPase essential both for efficient CtsR-dependent gene derepression during heat stress and for rerepression. Together with ClpP, degrades the global regulator CtsR after heat shock. Is also involved in disaggregation of heat-denatured proteins. Has thus

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=304, Percent_Identity=36.8421052631579, Blast_Score=180, Evalue=4e-45,
Organism=Escherichia coli, GI1788943, Length=696, Percent_Identity=48.9942528735632, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI1787109, Length=626, Percent_Identity=43.7699680511182, Blast_Score=518, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6320464, Length=699, Percent_Identity=40.7725321888412, Blast_Score=504, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6323002, Length=682, Percent_Identity=40.9090909090909, Blast_Score=463, Evalue=1e-131,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR001943 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small; PF02151 UVR [H]

EC number: NA

Molecular weight: Translated: 79295; Mature: 79295

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50151 UVR ; PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCQACQQREATVFVNLQWNGEKKQLHLCHTCYEKQKQQLSIPINFGFSPFSFDDWFTDH
CCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHH
FATANAQAVGPETAAKRPQRHGGGFLDQFGRNLTQMAKAGLIDPVIGRDKEIARVIEILN
HCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
RRNKNNPVLIGEPGVGKTAIVEGLALKIGEGQVPEKLLNKEVYLLDVASLVANTGIRGQF
CCCCCCCEEEECCCCCHHHHHHHHHEECCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCHH
EERMKRLITELQERKNIILFIDEIHLLVGAGAAEGSMDAGNILKPALARGELQVVGATTL
HHHHHHHHHHHHHCCCEEEEEEEEHHHHCCCCCCCCCCCCCHHHHHHCCCCEEEEEHHHH
KEYRQIEKDAALERRFQPVIVHEPTVDEAIAILRGIQPKYEQFHHVRYTDEAIEACVKLA
HHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHH
HRYIQDRFLPDKAIDLLDEAGSKANLRLGPTDEKQLQERLMQIAKEKDQAAKEENYELAA
HHHHHHHCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KLRDEELKLEKQLEQGVTQEHPVVDVADIERIIADKTGIPVGKLQADEKEKMKHLEDNLA
HHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
KKVIGQAEAVKKVAKAIRRSRAGLKAKHRPVGSFLFVGPTGVGKTELAKTLAEELFGTKD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCC
SMIRLDMSEYMEKHSVSKLIGSPPGYVGFEEAGQLTEKVRRNPYSIILLDEIEKAHPDVQ
CEEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECHHHHCCHHH
HIFLQILEDGRLTDSQGRTVSFKDTVIIATSNAGVTDKKITVGFEKQSGGASSILDSLSA
HHHHHHHHCCCCCCCCCCEEEECCEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHH
YFKPEFLNRFDAIIEFKPLEKAHLLQIVDLMLDDVKTAMREQGIELKVTEAAKEKLAELG
HHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCC
YHPAFGARPLRRVIQEHVEDNIADCLLDANQPVHTIRVDVNDGAIVAQIAS
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECC
>Mature Secondary Structure
MRCQACQQREATVFVNLQWNGEKKQLHLCHTCYEKQKQQLSIPINFGFSPFSFDDWFTDH
CCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHH
FATANAQAVGPETAAKRPQRHGGGFLDQFGRNLTQMAKAGLIDPVIGRDKEIARVIEILN
HCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
RRNKNNPVLIGEPGVGKTAIVEGLALKIGEGQVPEKLLNKEVYLLDVASLVANTGIRGQF
CCCCCCCEEEECCCCCHHHHHHHHHEECCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCHH
EERMKRLITELQERKNIILFIDEIHLLVGAGAAEGSMDAGNILKPALARGELQVVGATTL
HHHHHHHHHHHHHCCCEEEEEEEEHHHHCCCCCCCCCCCCCHHHHHHCCCCEEEEEHHHH
KEYRQIEKDAALERRFQPVIVHEPTVDEAIAILRGIQPKYEQFHHVRYTDEAIEACVKLA
HHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHH
HRYIQDRFLPDKAIDLLDEAGSKANLRLGPTDEKQLQERLMQIAKEKDQAAKEENYELAA
HHHHHHHCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KLRDEELKLEKQLEQGVTQEHPVVDVADIERIIADKTGIPVGKLQADEKEKMKHLEDNLA
HHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
KKVIGQAEAVKKVAKAIRRSRAGLKAKHRPVGSFLFVGPTGVGKTELAKTLAEELFGTKD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCC
SMIRLDMSEYMEKHSVSKLIGSPPGYVGFEEAGQLTEKVRRNPYSIILLDEIEKAHPDVQ
CEEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECHHHHCCHHH
HIFLQILEDGRLTDSQGRTVSFKDTVIIATSNAGVTDKKITVGFEKQSGGASSILDSLSA
HHHHHHHHCCCCCCCCCCEEEECCEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHH
YFKPEFLNRFDAIIEFKPLEKAHLLQIVDLMLDDVKTAMREQGIELKVTEAAKEKLAELG
HHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCC
YHPAFGARPLRRVIQEHVEDNIADCLLDANQPVHTIRVDVNDGAIVAQIAS
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]