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The map label for this gene is mtnU [H]

Identifier: 138894509

GI number: 138894509

Start: 914295

End: 915110

Strand: Reverse

Name: mtnU [H]

Synonym: GTNG_0839

Alternate gene names: 138894509

Gene position: 915110-914295 (Counterclockwise)

Preceding gene: 138894515

Following gene: 138894508

Centisome position: 25.78

GC content: 52.33

Gene sequence:

>816_bases
ATGACCATCCGCATCGCTTGCCTCCAATTGGATATCGCGTTTGGTGACCCGCAAAAAAACGAACAGCAAGCCGAAAAAGC
CGTGGAACTCGCCGTCAAAGAAGGAGCCGATATCATCGTTCTTCCGGAGCTGTGGACGACCGGTTATGACTTGACGCGCT
TAGATGAAATCGCTGATGACAATGCCGAACGGGCGAAAACGTTCGCCTCCCGATTGGCACAAACATACGGCATCCATTTT
GTGGCCGGCTCAGTCGCTAAAAAAACGGACGCCGGCGTAACGAACACAATGATTGTCGCCAATCGAAACGGCCAAATCAT
CAAGGAATATAGCAAAGTTCATTTGTTTCAGCTGATGGATGAGCATTTGCATTTGCAGTCAGGAAACGAGCCAGGCTTGT
TTTCGCTTGATGAGTTGTCGTGTGCCGGGGTCATTTGCTATGACATCCGCTTTCCGGAATGGATTCGCACCCATGCCTTG
TCTGGAGCCGAATTGTTGTTTGTTGTCGCCGAATGGCCGCTTCCTCGCCTTCACCATTGGAGGACGTTGCTGATGGCGCG
GGCGATCGAAAACCAATGCTATGTCGTCGCCTGCAATCGAGCCGGAAGCGACCCGAACAACGTCTTTGCTGGCCATTCGC
TCATCATCGATCCATGGGGGGAAATCCTCGCTGAGGCGGATGAGGACCCAGGCATCCTTCTAGCCGACATCGACCCGGCA
CTCGTCAGAGATGTTCGTTCGCGCATCCCCGTATTTGCTGACCGCCGGCCGCGCCTTTATGAAGAGACGACAAAAAAATT
TTTTAAAACGTATTGA

Upstream 100 bases:

>100_bases
TCGCCCACACCTTCTCAAAAATTTCTTGAAATTCTAGTGCAAAATCATATATCGTAAAAAGCAAAATGTAAAGTAAATTT
TTTCATTGAGGTGAAAAACA

Downstream 100 bases:

>100_bases
CAATCGGCCTGCTTAAGCTGTATTATTCGAATCAAGCAACCGAACAATTGCCAACATCACAAAAATTCCAAAAACTAAAT
AAGCAGATTCGGTACTCTTA

Product: Nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTIRIACLQLDIAFGDPQKNEQQAEKAVELAVKEGADIIVLPELWTTGYDLTRLDEIADDNAERAKTFASRLAQTYGIHF
VAGSVAKKTDAGVTNTMIVANRNGQIIKEYSKVHLFQLMDEHLHLQSGNEPGLFSLDELSCAGVICYDIRFPEWIRTHAL
SGAELLFVVAEWPLPRLHHWRTLLMARAIENQCYVVACNRAGSDPNNVFAGHSLIIDPWGEILAEADEDPGILLADIDPA
LVRDVRSRIPVFADRRPRLYEETTKKFFKTY

Sequences:

>Translated_271_residues
MTIRIACLQLDIAFGDPQKNEQQAEKAVELAVKEGADIIVLPELWTTGYDLTRLDEIADDNAERAKTFASRLAQTYGIHF
VAGSVAKKTDAGVTNTMIVANRNGQIIKEYSKVHLFQLMDEHLHLQSGNEPGLFSLDELSCAGVICYDIRFPEWIRTHAL
SGAELLFVVAEWPLPRLHHWRTLLMARAIENQCYVVACNRAGSDPNNVFAGHSLIIDPWGEILAEADEDPGILLADIDPA
LVRDVRSRIPVFADRRPRLYEETTKKFFKTY
>Mature_270_residues
TIRIACLQLDIAFGDPQKNEQQAEKAVELAVKEGADIIVLPELWTTGYDLTRLDEIADDNAERAKTFASRLAQTYGIHFV
AGSVAKKTDAGVTNTMIVANRNGQIIKEYSKVHLFQLMDEHLHLQSGNEPGLFSLDELSCAGVICYDIRFPEWIRTHALS
GAELLFVVAEWPLPRLHHWRTLLMARAIENQCYVVACNRAGSDPNNVFAGHSLIIDPWGEILAEADEDPGILLADIDPAL
VRDVRSRIPVFADRRPRLYEETTKKFFKTY

Specific function: May be involved in a regulatory step in the methylthioribose (MTR) recycling pathway [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=274, Percent_Identity=30.2919708029197, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI297632348, Length=272, Percent_Identity=31.9852941176471, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI297632350, Length=272, Percent_Identity=31.9852941176471, Blast_Score=114, Evalue=7e-26,
Organism=Homo sapiens, GI5031947, Length=272, Percent_Identity=31.9852941176471, Blast_Score=114, Evalue=7e-26,
Organism=Escherichia coli, GI1786412, Length=255, Percent_Identity=35.6862745098039, Blast_Score=152, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17556280, Length=281, Percent_Identity=32.7402135231317, Blast_Score=120, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6323383, Length=282, Percent_Identity=31.9148936170213, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6322335, Length=304, Percent_Identity=27.9605263157895, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21355835, Length=275, Percent_Identity=32, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17933642, Length=261, Percent_Identity=28.735632183908, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 30462; Mature: 30331

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRIACLQLDIAFGDPQKNEQQAEKAVELAVKEGADIIVLPELWTTGYDLTRLDEIADD
CEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCC
NAERAKTFASRLAQTYGIHFVAGSVAKKTDAGVTNTMIVANRNGQIIKEYSKVHLFQLMD
CHHHHHHHHHHHHHHHCEEEEECCHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
EHLHLQSGNEPGLFSLDELSCAGVICYDIRFPEWIRTHALSGAELLFVVAEWPLPRLHHW
HHHEECCCCCCCEEEECCCCCCCEEEEECCCHHHHHHHCCCCCEEEEEEECCCCCHHHHH
RTLLMARAIENQCYVVACNRAGSDPNNVFAGHSLIIDPWGEILAEADEDPGILLADIDPA
HHHHHHHHHCCCEEEEEECCCCCCCCCEEECCEEEECCHHHHHHCCCCCCCEEEECCCHH
LVRDVRSRIPVFADRRPRLYEETTKKFFKTY
HHHHHHHHCCEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIRIACLQLDIAFGDPQKNEQQAEKAVELAVKEGADIIVLPELWTTGYDLTRLDEIADD
EEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCC
NAERAKTFASRLAQTYGIHFVAGSVAKKTDAGVTNTMIVANRNGQIIKEYSKVHLFQLMD
CHHHHHHHHHHHHHHHCEEEEECCHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
EHLHLQSGNEPGLFSLDELSCAGVICYDIRFPEWIRTHALSGAELLFVVAEWPLPRLHHW
HHHEECCCCCCCEEEECCCCCCCEEEEECCCHHHHHHHCCCCCEEEEEEECCCCCHHHHH
RTLLMARAIENQCYVVACNRAGSDPNNVFAGHSLIIDPWGEILAEADEDPGILLADIDPA
HHHHHHHHHCCCEEEEEECCCCCCCCCEEECCEEEECCHHHHHHCCCCCCCEEEECCCHH
LVRDVRSRIPVFADRRPRLYEETTKKFFKTY
HHHHHHHHCCEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 12022921 [H]