Click here to switch to the map view.

The map label for this gene is purR [H]

Identifier: 138894492

GI number: 138894492

Start: 895445

End: 896461

Strand: Direct

Name: purR [H]

Synonym: GTNG_0822

Alternate gene names: 138894492

Gene position: 895445-896461 (Clockwise)

Preceding gene: 138894490

Following gene: 138894493

Centisome position: 25.22

GC content: 47.79

Gene sequence:

>1017_bases
GTGACAACGATTAAAGATATTGCCAAAGCGGCAGGAGTTTCGATTACTACCGTTTCACGTGCGTTAAACGGTTATTCCGA
TGTGAATGAAAACACGAGACAAAAGATCATTGATATCGCTAGGCAACTAAACTACATCCCGAATACGCTCGCACGCGGGC
TTGTGATGAATAAATCGAAAACGATCGGATTGCTCGTTTCCGGACTGACGAAGGAAAGCGCCAAGGACAATTTTACGTTT
GAGGTGCTAGCAGGTGTCAACGAATATGTCAGTGAAGTCGATTATGATATGGTGCTGTTCAGTACGACATCGACGAAACA
GCGGGAAAAAACGTACACCCAACTTTGCCGGGAGCGCCGTGTCGACGGAGCGATTTTGCAGGGGATTCGCACCGATGACC
CATACTTGCACGAGGTCGTGGAGAGCGATATTCCGTGCGTTTTAATCGATATTCCAATCGAGTCGCGGACGGTTGGTTAT
GTCACAACAGATAATGTACTTGGGGCGAAAAAGGCGGTGCGCCATTTAATTGAACTCGGCCATAAACGAATTGCTATGAT
TAACGGCTATGAATACGCTTTTGTGAGCGAACAGCGATTGAAAGGATTCAAGGAGGCCCTGCTTGAAGCTGGGTTGCCGG
TGCGGGAATACTGGATTGCCAATGGAGCGTTTCGTGAAGAAATTGCTGAGCAGGAAGCGTTGAGGCTGCTTCAACACTAC
CCAGAAATAACGGCGTTCTTTTGCGCCAGCGATTTAATGGCGCTAGGCGTGATTAAAGCAGTAAAACGGCTTGGAAAGCG
AGTGCCGGATGATGTTGCGGTCATCGGTTATGACGACATTATTTTAGCCTCATATTCCTCGCCTTCGCTGTCAACCATCG
CACAGGATAAGTTCGCTATGGGGTATGAAGCTGCCAAGTTGCTCATCGCTATGCTGGAAGGAAAGGCGGATTCGCATATC
AAAATTTTACAAACGGAGCTAAAAGCTCGGGAATCGACAGTCAAGGGAATCAAGTAG

Upstream 100 bases:

>100_bases
TAAAGGGAGGATTTGTTGTGAAAAAATTATTGCAAACGTTTTCGGTTCGTGGTTATAATAAAATTAACAACAGGATCCGA
AACGTTTTGGAGGAATAGCA

Downstream 100 bases:

>100_bases
TCGCCGTGTTATTGGTGACGGAAGGAGGGAAAAGATTTTTTTTGCGAAAAACCGAAAACGTTTCGGATAAAAAAGGGAAT
AGATCCATTAATTAAAACGC

Product: transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MTTIKDIAKAAGVSITTVSRALNGYSDVNENTRQKIIDIARQLNYIPNTLARGLVMNKSKTIGLLVSGLTKESAKDNFTF
EVLAGVNEYVSEVDYDMVLFSTTSTKQREKTYTQLCRERRVDGAILQGIRTDDPYLHEVVESDIPCVLIDIPIESRTVGY
VTTDNVLGAKKAVRHLIELGHKRIAMINGYEYAFVSEQRLKGFKEALLEAGLPVREYWIANGAFREEIAEQEALRLLQHY
PEITAFFCASDLMALGVIKAVKRLGKRVPDDVAVIGYDDIILASYSSPSLSTIAQDKFAMGYEAAKLLIAMLEGKADSHI
KILQTELKARESTVKGIK

Sequences:

>Translated_338_residues
MTTIKDIAKAAGVSITTVSRALNGYSDVNENTRQKIIDIARQLNYIPNTLARGLVMNKSKTIGLLVSGLTKESAKDNFTF
EVLAGVNEYVSEVDYDMVLFSTTSTKQREKTYTQLCRERRVDGAILQGIRTDDPYLHEVVESDIPCVLIDIPIESRTVGY
VTTDNVLGAKKAVRHLIELGHKRIAMINGYEYAFVSEQRLKGFKEALLEAGLPVREYWIANGAFREEIAEQEALRLLQHY
PEITAFFCASDLMALGVIKAVKRLGKRVPDDVAVIGYDDIILASYSSPSLSTIAQDKFAMGYEAAKLLIAMLEGKADSHI
KILQTELKARESTVKGIK
>Mature_337_residues
TTIKDIAKAAGVSITTVSRALNGYSDVNENTRQKIIDIARQLNYIPNTLARGLVMNKSKTIGLLVSGLTKESAKDNFTFE
VLAGVNEYVSEVDYDMVLFSTTSTKQREKTYTQLCRERRVDGAILQGIRTDDPYLHEVVESDIPCVLIDIPIESRTVGYV
TTDNVLGAKKAVRHLIELGHKRIAMINGYEYAFVSEQRLKGFKEALLEAGLPVREYWIANGAFREEIAEQEALRLLQHYP
EITAFFCASDLMALGVIKAVKRLGKRVPDDVAVIGYDDIILASYSSPSLSTIAQDKFAMGYEAAKLLIAMLEGKADSHIK
ILQTELKARESTVKGIK

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=34.1317365269461, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1787948, Length=339, Percent_Identity=33.0383480825959, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1790194, Length=311, Percent_Identity=34.7266881028939, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787580, Length=314, Percent_Identity=28.6624203821656, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789068, Length=335, Percent_Identity=28.955223880597, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1788474, Length=318, Percent_Identity=29.559748427673, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1789202, Length=296, Percent_Identity=29.0540540540541, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1786540, Length=344, Percent_Identity=27.906976744186, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787906, Length=268, Percent_Identity=28.7313432835821, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48994940, Length=322, Percent_Identity=29.5031055900621, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790715, Length=324, Percent_Identity=25.9259259259259, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790689, Length=333, Percent_Identity=24.024024024024, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786268, Length=315, Percent_Identity=21.5873015873016, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37531; Mature: 37400

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIKDIAKAAGVSITTVSRALNGYSDVNENTRQKIIDIARQLNYIPNTLARGLVMNKSK
CCCHHHHHHHHCCHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCC
TIGLLVSGLTKESAKDNFTFEVLAGVNEYVSEVDYDMVLFSTTSTKQREKTYTQLCRERR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHC
VDGAILQGIRTDDPYLHEVVESDIPCVLIDIPIESRTVGYVTTDNVLGAKKAVRHLIELG
CCHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHC
HKRIAMINGYEYAFVSEQRLKGFKEALLEAGLPVREYWIANGAFREEIAEQEALRLLQHY
CCEEEEECCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHC
PEITAFFCASDLMALGVIKAVKRLGKRVPDDVAVIGYDDIILASYSSPSLSTIAQDKFAM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHH
GYEAAKLLIAMLEGKADSHIKILQTELKARESTVKGIK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTIKDIAKAAGVSITTVSRALNGYSDVNENTRQKIIDIARQLNYIPNTLARGLVMNKSK
CCHHHHHHHHCCHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCC
TIGLLVSGLTKESAKDNFTFEVLAGVNEYVSEVDYDMVLFSTTSTKQREKTYTQLCRERR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHC
VDGAILQGIRTDDPYLHEVVESDIPCVLIDIPIESRTVGYVTTDNVLGAKKAVRHLIELG
CCHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHC
HKRIAMINGYEYAFVSEQRLKGFKEALLEAGLPVREYWIANGAFREEIAEQEALRLLQHY
CCEEEEECCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHC
PEITAFFCASDLMALGVIKAVKRLGKRVPDDVAVIGYDDIILASYSSPSLSTIAQDKFAM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHH
GYEAAKLLIAMLEGKADSHIKILQTELKARESTVKGIK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA