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The map label for this gene is shiA [H]

Identifier: 138894476

GI number: 138894476

Start: 878693

End: 879985

Strand: Direct

Name: shiA [H]

Synonym: GTNG_0806

Alternate gene names: 138894476

Gene position: 878693-879985 (Clockwise)

Preceding gene: 138894473

Following gene: 138894477

Centisome position: 24.75

GC content: 50.19

Gene sequence:

>1293_bases
ATGGATAAAGGGGTATCTAAACGGGTTTTAGTCGCGAGTCTCGTCGGCAGTTCAATTGAATGGTTTGACTACTTTCTGTA
TGGAACGGTCGCAGCACTTGTGTTCAATCAATTGTTTTTTCCAAGTGAAGACCCAACTGTCGGCTTGCTCTTATCATATG
CATCATTTGCCCTTTCATTTTTCATTCGCCCGTTAGGTGGCATTGTGTTTAGTCATATCGGCGATAAAATCGGGCGAAAG
AAGACGCTTGTTTTGACGTTAAGCCTCATGGGGATCGCCACGGCCGGCATGGGGCTGTTGCCAACGTATGAAACGATCGG
CATATGGGCGCCCGTGCTGCTTATTTTGCTTCGCCTCATCCAAGGGATTGGTCTCGGCGGTGAGTGGGGCGGGGCGCTGT
TGCTCGCGGTCGAATACGCACCGAAAGAAAAGCGCGGCTTATTCGGCAGCATCCCGCAAATGGGCGTGACGATCGGCTTG
CTTCTTGGCACCTTTGTAATGTCGCTCATGACGCTTATGCCGGACGAGACGTTTATGTCATGGGGGTGGCGCATTCCTTT
TCTGTTAAGTGCGTTGCTTGTCGTTTTTGGATTGTGGGTGCGCCATGGCATTGATGAAACACCTTCGTTTAAGAAGGTGC
AAGCAACTGGACGAGTGGCGAACGTGCCGCTTATTGAGACACTGCGAACTCAATGGAAGGAAGTATTGATCGCAGTCGGC
GGCAAAGTGGTTGAAACAGCGCCGTTTTACATTTTTGGAACGTTTATCGTTTCATATGCAACAACGAACTTAGGTTTCTC
GAAAACAGCAGCATTGAATGCAGTAACTGTGGCGACAGTCGTGACGACGATTCTGATTCCACTGATGGGTAAATGGTCGG
ACCGTTACGGCCGCAAGCCGCTTTATATAGCGGGGACGATCGGGATGATCGTTTATGCGTTCCCATATTTTTGGCTTTTG
CAACAAAAATCAGTCGCCTTGTTGATGATAGCGACGATCATTGGTCTTGGCATCATTTGGGCACCGATTACGGCTGTGCT
CGGCACGATGTTTTCGGAAATTTTCCCGTCCAATGTTCGATACACGGGCATTTCGCTCGGCTACCAAATCGGTGCTGCAT
TAGCGGGGGGAACGGCGCCTTTGATCGCTACAGCCTTGCTTGCGGCGTACGGGAACTCTTATGTTCCGGTCGCCTTGTAC
ATTATATTCGTATCGCTTGTTTCGCTGATCGCAGTTTGGTCGGTGCGCGACCGCAAACATGAGGAACTTGATGAGGAAGA
CGGAATCGCGTAA

Upstream 100 bases:

>100_bases
AAAGTTGATGAATAATCAGAATGTTTATTATTTACTGTCTGTATAATATTTTTATATAATTTATTATGGATAGACTGTCT
ATCAACAGGGGGGAGAACTG

Downstream 100 bases:

>100_bases
CGCGGTTCCGTTTCGTTCTAACGGAGGGAGAATCATGCTTATTTTGTCGGAGAAAGAAATTCAGCGTCTGTATACGATGG
AGGATGCTATTTCCGATGTA

Product: putative transport protein

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MDKGVSKRVLVASLVGSSIEWFDYFLYGTVAALVFNQLFFPSEDPTVGLLLSYASFALSFFIRPLGGIVFSHIGDKIGRK
KTLVLTLSLMGIATAGMGLLPTYETIGIWAPVLLILLRLIQGIGLGGEWGGALLLAVEYAPKEKRGLFGSIPQMGVTIGL
LLGTFVMSLMTLMPDETFMSWGWRIPFLLSALLVVFGLWVRHGIDETPSFKKVQATGRVANVPLIETLRTQWKEVLIAVG
GKVVETAPFYIFGTFIVSYATTNLGFSKTAALNAVTVATVVTTILIPLMGKWSDRYGRKPLYIAGTIGMIVYAFPYFWLL
QQKSVALLMIATIIGLGIIWAPITAVLGTMFSEIFPSNVRYTGISLGYQIGAALAGGTAPLIATALLAAYGNSYVPVALY
IIFVSLVSLIAVWSVRDRKHEELDEEDGIA

Sequences:

>Translated_430_residues
MDKGVSKRVLVASLVGSSIEWFDYFLYGTVAALVFNQLFFPSEDPTVGLLLSYASFALSFFIRPLGGIVFSHIGDKIGRK
KTLVLTLSLMGIATAGMGLLPTYETIGIWAPVLLILLRLIQGIGLGGEWGGALLLAVEYAPKEKRGLFGSIPQMGVTIGL
LLGTFVMSLMTLMPDETFMSWGWRIPFLLSALLVVFGLWVRHGIDETPSFKKVQATGRVANVPLIETLRTQWKEVLIAVG
GKVVETAPFYIFGTFIVSYATTNLGFSKTAALNAVTVATVVTTILIPLMGKWSDRYGRKPLYIAGTIGMIVYAFPYFWLL
QQKSVALLMIATIIGLGIIWAPITAVLGTMFSEIFPSNVRYTGISLGYQIGAALAGGTAPLIATALLAAYGNSYVPVALY
IIFVSLVSLIAVWSVRDRKHEELDEEDGIA
>Mature_430_residues
MDKGVSKRVLVASLVGSSIEWFDYFLYGTVAALVFNQLFFPSEDPTVGLLLSYASFALSFFIRPLGGIVFSHIGDKIGRK
KTLVLTLSLMGIATAGMGLLPTYETIGIWAPVLLILLRLIQGIGLGGEWGGALLLAVEYAPKEKRGLFGSIPQMGVTIGL
LLGTFVMSLMTLMPDETFMSWGWRIPFLLSALLVVFGLWVRHGIDETPSFKKVQATGRVANVPLIETLRTQWKEVLIAVG
GKVVETAPFYIFGTFIVSYATTNLGFSKTAALNAVTVATVVTTILIPLMGKWSDRYGRKPLYIAGTIGMIVYAFPYFWLL
QQKSVALLMIATIIGLGIIWAPITAVLGTMFSEIFPSNVRYTGISLGYQIGAALAGGTAPLIATALLAAYGNSYVPVALY
IIFVSLVSLIAVWSVRDRKHEELDEEDGIA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=418, Percent_Identity=39.9521531100478, Blast_Score=329, Evalue=2e-91,
Organism=Escherichia coli, GI1790550, Length=433, Percent_Identity=33.7182448036951, Blast_Score=271, Evalue=8e-74,
Organism=Escherichia coli, GI1789941, Length=426, Percent_Identity=41.0798122065728, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI1788942, Length=379, Percent_Identity=33.245382585752, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI87082231, Length=265, Percent_Identity=25.2830188679245, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082404, Length=368, Percent_Identity=23.0978260869565, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46615; Mature: 46615

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS00359 RIBOSOMAL_L11 ; PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKGVSKRVLVASLVGSSIEWFDYFLYGTVAALVFNQLFFPSEDPTVGLLLSYASFALSF
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
FIRPLGGIVFSHIGDKIGRKKTLVLTLSLMGIATAGMGLLPTYETIGIWAPVLLILLRLI
HHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
QGIGLGGEWGGALLLAVEYAPKEKRGLFGSIPQMGVTIGLLLGTFVMSLMTLMPDETFMS
HHCCCCCCCCCEEEEEEECCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
WGWRIPFLLSALLVVFGLWVRHGIDETPSFKKVQATGRVANVPLIETLRTQWKEVLIAVG
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
GKVVETAPFYIFGTFIVSYATTNLGFSKTAALNAVTVATVVTTILIPLMGKWSDRYGRKP
CHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
LYIAGTIGMIVYAFPYFWLLQQKSVALLMIATIIGLGIIWAPITAVLGTMFSEIFPSNVR
EEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
YTGISLGYQIGAALAGGTAPLIATALLAAYGNSYVPVALYIIFVSLVSLIAVWSVRDRKH
EEEEEHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
EELDEEDGIA
HCCCCCCCCC
>Mature Secondary Structure
MDKGVSKRVLVASLVGSSIEWFDYFLYGTVAALVFNQLFFPSEDPTVGLLLSYASFALSF
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
FIRPLGGIVFSHIGDKIGRKKTLVLTLSLMGIATAGMGLLPTYETIGIWAPVLLILLRLI
HHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
QGIGLGGEWGGALLLAVEYAPKEKRGLFGSIPQMGVTIGLLLGTFVMSLMTLMPDETFMS
HHCCCCCCCCCEEEEEEECCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
WGWRIPFLLSALLVVFGLWVRHGIDETPSFKKVQATGRVANVPLIETLRTQWKEVLIAVG
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
GKVVETAPFYIFGTFIVSYATTNLGFSKTAALNAVTVATVVTTILIPLMGKWSDRYGRKP
CHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
LYIAGTIGMIVYAFPYFWLLQQKSVALLMIATIIGLGIIWAPITAVLGTMFSEIFPSNVR
EEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
YTGISLGYQIGAALAGGTAPLIATALLAAYGNSYVPVALYIIFVSLVSLIAVWSVRDRKH
EEEEEHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
EELDEEDGIA
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]