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The map label for this gene is asnB [H]

Identifier: 138894460

GI number: 138894460

Start: 862889

End: 864796

Strand: Direct

Name: asnB [H]

Synonym: GTNG_0790

Alternate gene names: 138894460

Gene position: 862889-864796 (Clockwise)

Preceding gene: 138894459

Following gene: 138894465

Centisome position: 24.3

GC content: 46.65

Gene sequence:

>1908_bases
ATGTGCGGCTTTATCGGTTGTGTTCACGATCATCCGCGAATAAGAAATAAAAAATGGAACGAAATGTTTCAACTAATGAA
CGGTCTTATTACCCATCGCGGTCCTGATGATGAAGGGTATTTTTTTGATGACTACGTGTCTTTCGGGTTTCGACGTTTAA
GCATTATCGATTTGGAAGCAGGGCATCAGCCGCTCTCTTACGAAAACGAACGCTACTGGATCATCTTTAACGGGGAAATC
TACAACTATATTGAATTGCGGGAAGAACTGTTGGCGAAGGGGCATCAATTCGCGACGCACTCTGATACTGAAGTGATTGT
TGCTCTTTATAGCGTGGAAAAAGAAAAAGCAGTCGAAAGGCTGCGCGGGATGTTTGCGTTTGTCATTTGGGACAAGCAAG
AGAAATCGGTGTTTGCGGCGCGGGACCCGTTTGGTATTAAGCCATTTTTTTATGCGGAACAAGAGGGTCGCACATTTTTT
GCTTCAGAAAAGAAAAGCATTTTGTTTGCGTTGGGGCATGAATCATTGGAGCATGATTCTCTACAACATTATTTAACCTT
CCAATACGTCCCTGAGCCATTGACGATGTCGTCTGGCATTTACAAGTTGGAACCAGGGCATTATATCAAGAAAAAAGTTG
GGGAACCGCTCACCGTTCACCGTTATTGGAAAGCATCCTTCCAGCCTGTTCGCCAATCGGAAGAGGAGCTTGTGAAACAA
ATTCGCGAGGCGCTGTTTGATTCGGTGAACGTGCATATGCGCAGCGATGTGCCGGTTGGAGCGTTTTTGTCAGGCGGGAT
TGACTCATCGTTGATCGTGGCCATCGCCAAGCAATTCCATCCGCATCTTAAGACATTTTCTGTCGGGTTTGAGCGGGAAG
GGTTCAGCGAAATTGATGTTGCCAAAGAAACGGCGGAGAAGCTCGGTGTGGACAATATCAGCTATGTCATCTCCCCGCAG
GAGTATATGGACGAGCTTCCGAAAATCATGTGGCATATGGATGACCCATTAGCTGATCCGGCAGCTGTGCCGCTCTATTT
TGTGGCCCGTGAGGCGAGAAAACATGTTACGGTCGTGCTTTCCGGTGAGGGGGCAGACGAGCTGTTTGGCGGTTACAATA
TTTATCGCGAACCGCAGTCACTGCGGCTGTTCGAGCGGATGCCAGACGTGGTGAAAGCTGTGTTGCGGATGATCGCGAAG
CTTCTTCCGGATGGAATGAAAGGAAAAAGTTTCTTAGAACGCGGGACGACGCCGCTCGAGGAACGGTATGTTGGCAATGC
GAAAATGTATAGCGAAAACGAAAAAGAGGCATTACTCAAAACATATCAAAAAGGGCGCGAGTATACAACGATTACGGCTC
CCTTTTATGAAGAAACGTCTCATTACCCTCCGGTCAACCGGATGCAATACATTGACATTCATACATGGCTAAGAGGCGAT
ATTTTATTAAAAGCGGATAAAATGACGATGGCTCATTCGCTCGAACTGCGGGTTCCATTTTTAGATCGAAAGGTGTTTGA
AGTCGCATCCAAAATTGCTCCTGACATGAAAACAGCCAACGGTACGACAAAATACATTTTGCGCAAGGCCGCTGAAGGCA
TCGTGCCGGATCATGTGTTAAACCGGAAGAAACTAGGGTTCCCGGTGCCGATTCGCCATTGGCTGAGAAATGAAATGTAT
GACTGGGCAAGACGACTTATCTATGAAAGTGAAACTGACCATTTGTTCCATAAGGATGTGCTATACCGTTTGCTCGATGA
GCACGCCCAGCAGAAAGCGGATCATAGTCGCAAGCTGTGGACCGTCTTGGCGTTTATGATCTGGCACCAAGTCTATGTAG
AAAAGAAATACGATTTTGCTTTATCTGTACAAGACGAAAAGTGGTCAACATCTGTTTTGGAGGCATAG

Upstream 100 bases:

>100_bases
TACATTCATCTTCTGTTGATTGGCCAAACTCTGCCTTTTATGAGCGTAAAGCAACAATCTTCTTTTAGAACGATGATACC
AAGGGGGAAGAATCAACATT

Downstream 100 bases:

>100_bases
CAGCGAACTGAAAAGGGGTCTCGGGAGTGTAGCCGGGACTCCTTTTTTGATGATTGCAAACAGAAAAGGAGTTCAGCAGT
GAAGGGGGGAGTCAGCTCCC

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MCGFIGCVHDHPRIRNKKWNEMFQLMNGLITHRGPDDEGYFFDDYVSFGFRRLSIIDLEAGHQPLSYENERYWIIFNGEI
YNYIELREELLAKGHQFATHSDTEVIVALYSVEKEKAVERLRGMFAFVIWDKQEKSVFAARDPFGIKPFFYAEQEGRTFF
ASEKKSILFALGHESLEHDSLQHYLTFQYVPEPLTMSSGIYKLEPGHYIKKKVGEPLTVHRYWKASFQPVRQSEEELVKQ
IREALFDSVNVHMRSDVPVGAFLSGGIDSSLIVAIAKQFHPHLKTFSVGFEREGFSEIDVAKETAEKLGVDNISYVISPQ
EYMDELPKIMWHMDDPLADPAAVPLYFVAREARKHVTVVLSGEGADELFGGYNIYREPQSLRLFERMPDVVKAVLRMIAK
LLPDGMKGKSFLERGTTPLEERYVGNAKMYSENEKEALLKTYQKGREYTTITAPFYEETSHYPPVNRMQYIDIHTWLRGD
ILLKADKMTMAHSLELRVPFLDRKVFEVASKIAPDMKTANGTTKYILRKAAEGIVPDHVLNRKKLGFPVPIRHWLRNEMY
DWARRLIYESETDHLFHKDVLYRLLDEHAQQKADHSRKLWTVLAFMIWHQVYVEKKYDFALSVQDEKWSTSVLEA

Sequences:

>Translated_635_residues
MCGFIGCVHDHPRIRNKKWNEMFQLMNGLITHRGPDDEGYFFDDYVSFGFRRLSIIDLEAGHQPLSYENERYWIIFNGEI
YNYIELREELLAKGHQFATHSDTEVIVALYSVEKEKAVERLRGMFAFVIWDKQEKSVFAARDPFGIKPFFYAEQEGRTFF
ASEKKSILFALGHESLEHDSLQHYLTFQYVPEPLTMSSGIYKLEPGHYIKKKVGEPLTVHRYWKASFQPVRQSEEELVKQ
IREALFDSVNVHMRSDVPVGAFLSGGIDSSLIVAIAKQFHPHLKTFSVGFEREGFSEIDVAKETAEKLGVDNISYVISPQ
EYMDELPKIMWHMDDPLADPAAVPLYFVAREARKHVTVVLSGEGADELFGGYNIYREPQSLRLFERMPDVVKAVLRMIAK
LLPDGMKGKSFLERGTTPLEERYVGNAKMYSENEKEALLKTYQKGREYTTITAPFYEETSHYPPVNRMQYIDIHTWLRGD
ILLKADKMTMAHSLELRVPFLDRKVFEVASKIAPDMKTANGTTKYILRKAAEGIVPDHVLNRKKLGFPVPIRHWLRNEMY
DWARRLIYESETDHLFHKDVLYRLLDEHAQQKADHSRKLWTVLAFMIWHQVYVEKKYDFALSVQDEKWSTSVLEA
>Mature_635_residues
MCGFIGCVHDHPRIRNKKWNEMFQLMNGLITHRGPDDEGYFFDDYVSFGFRRLSIIDLEAGHQPLSYENERYWIIFNGEI
YNYIELREELLAKGHQFATHSDTEVIVALYSVEKEKAVERLRGMFAFVIWDKQEKSVFAARDPFGIKPFFYAEQEGRTFF
ASEKKSILFALGHESLEHDSLQHYLTFQYVPEPLTMSSGIYKLEPGHYIKKKVGEPLTVHRYWKASFQPVRQSEEELVKQ
IREALFDSVNVHMRSDVPVGAFLSGGIDSSLIVAIAKQFHPHLKTFSVGFEREGFSEIDVAKETAEKLGVDNISYVISPQ
EYMDELPKIMWHMDDPLADPAAVPLYFVAREARKHVTVVLSGEGADELFGGYNIYREPQSLRLFERMPDVVKAVLRMIAK
LLPDGMKGKSFLERGTTPLEERYVGNAKMYSENEKEALLKTYQKGREYTTITAPFYEETSHYPPVNRMQYIDIHTWLRGD
ILLKADKMTMAHSLELRVPFLDRKVFEVASKIAPDMKTANGTTKYILRKAAEGIVPDHVLNRKKLGFPVPIRHWLRNEMY
DWARRLIYESETDHLFHKDVLYRLLDEHAQQKADHSRKLWTVLAFMIWHQVYVEKKYDFALSVQDEKWSTSVLEA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=370, Percent_Identity=28.6486486486486, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI168229252, Length=370, Percent_Identity=28.6486486486486, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI168229250, Length=370, Percent_Identity=28.6486486486486, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI296010848, Length=362, Percent_Identity=29.0055248618785, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI296010852, Length=290, Percent_Identity=27.9310344827586, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI296010850, Length=290, Percent_Identity=27.9310344827586, Blast_Score=98, Evalue=3e-20,
Organism=Escherichia coli, GI1786889, Length=408, Percent_Identity=32.843137254902, Blast_Score=180, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI25147557, Length=367, Percent_Identity=32.9700272479564, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71993933, Length=367, Percent_Identity=32.9700272479564, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17560178, Length=374, Percent_Identity=29.6791443850267, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25147560, Length=288, Percent_Identity=31.5972222222222, Blast_Score=119, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6321563, Length=501, Percent_Identity=27.1457085828343, Blast_Score=150, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6325403, Length=499, Percent_Identity=26.8537074148297, Blast_Score=150, Evalue=8e-37,
Organism=Drosophila melanogaster, GI45553209, Length=353, Percent_Identity=32.8611898016997, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 73915; Mature: 73915

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGFIGCVHDHPRIRNKKWNEMFQLMNGLITHRGPDDEGYFFDDYVSFGFRRLSIIDLEA
CCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEHHHHHCCCEEEEEEEECC
GHQPLSYENERYWIIFNGEIYNYIELREELLAKGHQFATHSDTEVIVALYSVEKEKAVER
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHH
LRGMFAFVIWDKQEKSVFAARDPFGIKPFFYAEQEGRTFFASEKKSILFALGHESLEHDS
HHCEEEEEEECCCCCCEEEECCCCCCCCEEEECCCCCEEEECCCCEEEEEECCCCCCHHH
LQHYLTFQYVPEPLTMSSGIYKLEPGHYIKKKVGEPLTVHRYWKASFQPVRQSEEELVKQ
HHHEEEEEECCCCCCCCCCEEEECCCHHHHHHCCCCEEEEHHHHHCCCHHHCCHHHHHHH
IREALFDSVNVHMRSDVPVGAFLSGGIDSSLIVAIAKQFHPHLKTFSVGFEREGFSEIDV
HHHHHHHCCCEEEECCCCCCHHHCCCCCHHHHHHHHHHHCCCHHHEECCCCCCCCCHHHH
AKETAEKLGVDNISYVISPQEYMDELPKIMWHMDDPLADPAAVPLYFVAREARKHVTVVL
HHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEEE
SGEGADELFGGYNIYREPQSLRLFERMPDVVKAVLRMIAKLLPDGMKGKSFLERGTTPLE
ECCCHHHHHCCCCEEECCHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHH
ERYVGNAKMYSENEKEALLKTYQKGREYTTITAPFYEETSHYPPVNRMQYIDIHTWLRGD
HHHCCCCHHCCCCHHHHHHHHHHCCCCEEEEECCHHHCCCCCCCCCCEEEEEEEHEECCC
ILLKADKMTMAHSLELRVPFLDRKVFEVASKIAPDMKTANGTTKYILRKAAEGIVPDHVL
EEEEECCHHHEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHH
NRKKLGFPVPIRHWLRNEMYDWARRLIYESETDHLFHKDVLYRLLDEHAQQKADHSRKLW
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVLAFMIWHQVYVEKKYDFALSVQDEKWSTSVLEA
HHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCC
>Mature Secondary Structure
MCGFIGCVHDHPRIRNKKWNEMFQLMNGLITHRGPDDEGYFFDDYVSFGFRRLSIIDLEA
CCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEHHHHHCCCEEEEEEEECC
GHQPLSYENERYWIIFNGEIYNYIELREELLAKGHQFATHSDTEVIVALYSVEKEKAVER
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHH
LRGMFAFVIWDKQEKSVFAARDPFGIKPFFYAEQEGRTFFASEKKSILFALGHESLEHDS
HHCEEEEEEECCCCCCEEEECCCCCCCCEEEECCCCCEEEECCCCEEEEEECCCCCCHHH
LQHYLTFQYVPEPLTMSSGIYKLEPGHYIKKKVGEPLTVHRYWKASFQPVRQSEEELVKQ
HHHEEEEEECCCCCCCCCCEEEECCCHHHHHHCCCCEEEEHHHHHCCCHHHCCHHHHHHH
IREALFDSVNVHMRSDVPVGAFLSGGIDSSLIVAIAKQFHPHLKTFSVGFEREGFSEIDV
HHHHHHHCCCEEEECCCCCCHHHCCCCCHHHHHHHHHHHCCCHHHEECCCCCCCCCHHHH
AKETAEKLGVDNISYVISPQEYMDELPKIMWHMDDPLADPAAVPLYFVAREARKHVTVVL
HHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEEE
SGEGADELFGGYNIYREPQSLRLFERMPDVVKAVLRMIAKLLPDGMKGKSFLERGTTPLE
ECCCHHHHHCCCCEEECCHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHH
ERYVGNAKMYSENEKEALLKTYQKGREYTTITAPFYEETSHYPPVNRMQYIDIHTWLRGD
HHHCCCCHHCCCCHHHHHHHHHHCCCCEEEEECCHHHCCCCCCCCCCEEEEEEEHEECCC
ILLKADKMTMAHSLELRVPFLDRKVFEVASKIAPDMKTANGTTKYILRKAAEGIVPDHVL
EEEEECCHHHEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHH
NRKKLGFPVPIRHWLRNEMYDWARRLIYESETDHLFHKDVLYRLLDEHAQQKADHSRKLW
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVLAFMIWHQVYVEKKYDFALSVQDEKWSTSVLEA
HHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]