Click here to switch to the map view.

The map label for this gene is tuf [H]

Identifier: 138893783

GI number: 138893783

Start: 123852

End: 125039

Strand: Direct

Name: tuf [H]

Synonym: GTNG_0104

Alternate gene names: 138893783

Gene position: 123852-125039 (Clockwise)

Preceding gene: 138893782

Following gene: 138893784

Centisome position: 3.49

GC content: 52.1

Gene sequence:

>1188_bases
ATGGCTAAAGCGAAATTTGAGCGTACGAAACCGCACGTCAACATTGGCACGATCGGCCACGTTGACCATGGGAAAACGAC
GTTGACAGCTGCGATCACGACAGTTCTTGCGAAACAAGGTAAAGCAGAAGCGAGAGCGTACGACCAAATCGACGCTGCTC
CGGAAGAGCGTGAACGCGGAATCACGATTTCGACGGCTCACGTTGAGTATGAAACAGAAAACCGTCACTATGCGCACGTT
GACTGCCCGGGCCACGCTGACTACGTGAAAAACATGATCACGGGCGCGGCGCAAATGGACGGCGCGATCCTTGTTGTATC
GGCTGCTGACGGTCCGATGCCGCAAACTCGCGAACACATTCTTCTTTCCCGCCAAGTCGGTGTTCCGTACATCGTTGTTT
TCTTGAACAAATGCGACATGGTGGACGACGAAGAATTGCTTGAACTCGTTGAAATGGAAGTTCGCGATCTTCTTTCTGAA
TATGACTTCCCGGGCGACGAAGTGCCGGTTATCAAAGGTTCGGCATTAAAAGCGCTCGAAGGCGATGCACAATGGGAAGA
AAAAATCGTTGAACTGATGAACGCGGTTGACGAGTACATCCCAACTCCGCAACGTGAAGTAGACAAACCGTTCATGATGC
CGGTTGAGGACGTCTTCTCGATCACGGGTCGTGGTACGGTTGCAACGGGCCGTGTTGAGCGCGGTACGTTAAAAGTTGGT
GACCCGGTTGAAATCATCGGTCTTTCGGACGAGCCGAAATCGACGACTGTTACGGGTGTAGAAATGTTCCGTAAGCTTCT
CGACCAAGCAGAAGCTGGTGACAACATCGGTGCGCTTCTCCGCGGTGTATCGCGTGACGAAGTTGAGCGCGGTCAAGTAT
TGGCGAAACCGGGCTCGATCACGCCACACACGAAATTTAAAGCACAAGTTTACGTTCTGACGAAAGAAGAAGGCGGACGC
CATACTCCGTTCTTCTCGAACTACCGTCCGCAATTCTACTTCCGTACAACGGACGTAACGGGCATCATCACGCTTCCAGA
AGGCGTTGAAATGGTTATGCCTGGCGACAACGTTGAAATGACGGTTGAACTGATCGCTCCGATCGCGATCGAAGAAGGTA
CGAAATTCTCGATCCGTGAAGGCGGCCGCACGGTTGGTGCTGGTTCCGTATCGGAAATCATTGAGTAA

Upstream 100 bases:

>100_bases
CTATAAATACTTATGTAAGACTTGGGAACATCTTGTTCCCAAGCATCCTATAATTACTTAACTATATCAATCCATATTTT
ATTTAAGGAGGATCTTTCTC

Downstream 100 bases:

>100_bases
TCAATGAAAAAAGGATGTCCAGTTGATGGACATCCTTTTTCTTTTAGCTAGACATTTCGTTTTATTAGAAGTCTAAGAAA
TTCTTAATCCGTGAAGGTGC

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITTVLAKQGKAEARAYDQIDAAPEERERGITISTAHVEYETENRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSE
YDFPGDEVPVIKGSALKALEGDAQWEEKIVELMNAVDEYIPTPQREVDKPFMMPVEDVFSITGRGTVATGRVERGTLKVG
DPVEIIGLSDEPKSTTVTGVEMFRKLLDQAEAGDNIGALLRGVSRDEVERGQVLAKPGSITPHTKFKAQVYVLTKEEGGR
HTPFFSNYRPQFYFRTTDVTGIITLPEGVEMVMPGDNVEMTVELIAPIAIEEGTKFSIREGGRTVGAGSVSEIIE

Sequences:

>Translated_395_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITTVLAKQGKAEARAYDQIDAAPEERERGITISTAHVEYETENRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSE
YDFPGDEVPVIKGSALKALEGDAQWEEKIVELMNAVDEYIPTPQREVDKPFMMPVEDVFSITGRGTVATGRVERGTLKVG
DPVEIIGLSDEPKSTTVTGVEMFRKLLDQAEAGDNIGALLRGVSRDEVERGQVLAKPGSITPHTKFKAQVYVLTKEEGGR
HTPFFSNYRPQFYFRTTDVTGIITLPEGVEMVMPGDNVEMTVELIAPIAIEEGTKFSIREGGRTVGAGSVSEIIE
>Mature_394_residues
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITTVLAKQGKAEARAYDQIDAAPEERERGITISTAHVEYETENRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSEY
DFPGDEVPVIKGSALKALEGDAQWEEKIVELMNAVDEYIPTPQREVDKPFMMPVEDVFSITGRGTVATGRVERGTLKVGD
PVEIIGLSDEPKSTTVTGVEMFRKLLDQAEAGDNIGALLRGVSRDEVERGQVLAKPGSITPHTKFKAQVYVLTKEEGGRH
TPFFSNYRPQFYFRTTDVTGIITLPEGVEMVMPGDNVEMTVELIAPIAIEEGTKFSIREGGRTVGAGSVSEIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=57.9746835443038, Blast_Score=477, Evalue=1e-135,
Organism=Homo sapiens, GI223555963, Length=443, Percent_Identity=32.0541760722348, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI5729864, Length=443, Percent_Identity=32.0541760722348, Blast_Score=172, Evalue=7e-43,
Organism=Homo sapiens, GI4503475, Length=354, Percent_Identity=32.4858757062147, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4503471, Length=447, Percent_Identity=28.8590604026846, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI194018522, Length=346, Percent_Identity=28.3236994219653, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI194097354, Length=346, Percent_Identity=28.6127167630058, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI194018520, Length=346, Percent_Identity=28.6127167630058, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI54607086, Length=333, Percent_Identity=30.6306306306306, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI46094014, Length=336, Percent_Identity=27.0833333333333, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI4503507, Length=274, Percent_Identity=29.1970802919708, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI157426893, Length=234, Percent_Identity=27.3504273504274, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=34.0909090909091, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI53729339, Length=227, Percent_Identity=30.8370044052863, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI53729337, Length=227, Percent_Identity=30.8370044052863, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI18390331, Length=147, Percent_Identity=30.6122448979592, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=75.8883248730965, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=75.8883248730965, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI2367247, Length=382, Percent_Identity=29.5811518324607, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI48994988, Length=277, Percent_Identity=33.2129963898917, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789108, Length=263, Percent_Identity=31.9391634980989, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788922, Length=331, Percent_Identity=25.3776435045317, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=52.75, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=45.0777202072539, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=454, Percent_Identity=31.2775330396476, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=454, Percent_Identity=31.2775330396476, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI32566303, Length=435, Percent_Identity=29.4252873563218, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32566629, Length=455, Percent_Identity=25.9340659340659, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=431, Percent_Identity=27.6102088167053, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=428, Percent_Identity=27.5700934579439, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=300, Percent_Identity=29, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=148, Percent_Identity=37.8378378378378, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=269, Percent_Identity=27.1375464684015, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=66.3316582914573, Blast_Score=546, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6325337, Length=348, Percent_Identity=32.7586206896552, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=348, Percent_Identity=32.7586206896552, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6320377, Length=346, Percent_Identity=29.1907514450867, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6322937, Length=347, Percent_Identity=25.3602305475504, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=272, Percent_Identity=27.9411764705882, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323098, Length=202, Percent_Identity=32.1782178217822, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=241, Percent_Identity=29.8755186721992, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281363316, Length=394, Percent_Identity=57.3604060913706, Blast_Score=477, Evalue=1e-135,
Organism=Drosophila melanogaster, GI17864358, Length=394, Percent_Identity=57.3604060913706, Blast_Score=477, Evalue=1e-135,
Organism=Drosophila melanogaster, GI19921738, Length=387, Percent_Identity=49.3540051679587, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45553807, Length=448, Percent_Identity=29.9107142857143, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI45553816, Length=448, Percent_Identity=29.9107142857143, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24651721, Length=448, Percent_Identity=29.9107142857143, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI17864154, Length=448, Percent_Identity=29.9107142857143, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24652838, Length=447, Percent_Identity=29.082774049217, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17137572, Length=447, Percent_Identity=29.082774049217, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=32.5657894736842, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI17137380, Length=337, Percent_Identity=29.673590504451, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45550900, Length=337, Percent_Identity=29.080118694362, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24647046, Length=267, Percent_Identity=28.4644194756554, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24647048, Length=267, Percent_Identity=28.4644194756554, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI78706572, Length=260, Percent_Identity=27.3076923076923, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582462, Length=165, Percent_Identity=32.1212121212121, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI28572034, Length=164, Percent_Identity=34.7560975609756, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 43324; Mature: 43193

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITTVLAKQGKAEARAYDQIDAAPEERERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCC
ITISTAHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
CEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSEYDFPGDEVPVIKGSALKALE
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHC
GDAQWEEKIVELMNAVDEYIPTPQREVDKPFMMPVEDVFSITGRGTVATGRVERGTLKVG
CCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHCCCCEEEECCEECCEEECC
DPVEIIGLSDEPKSTTVTGVEMFRKLLDQAEAGDNIGALLRGVSRDEVERGQVLAKPGSI
CCEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHCCCEEECCCCC
TPHTKFKAQVYVLTKEEGGRHTPFFSNYRPQFYFRTTDVTGIITLPEGVEMVMPGDNVEM
CCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCEE
TVELIAPIAIEEGTKFSIREGGRTVGAGSVSEIIE
EEEEEEEEEECCCCEEEECCCCCEECCCCHHHHCC
>Mature Secondary Structure 
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITTVLAKQGKAEARAYDQIDAAPEERERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCC
ITISTAHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
CEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSEYDFPGDEVPVIKGSALKALE
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHC
GDAQWEEKIVELMNAVDEYIPTPQREVDKPFMMPVEDVFSITGRGTVATGRVERGTLKVG
CCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHCCCCEEEECCEECCEEECC
DPVEIIGLSDEPKSTTVTGVEMFRKLLDQAEAGDNIGALLRGVSRDEVERGQVLAKPGSI
CCEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHCCCEEECCCCC
TPHTKFKAQVYVLTKEEGGRHTPFFSNYRPQFYFRTTDVTGIITLPEGVEMVMPGDNVEM
CCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCEE
TVELIAPIAIEEGTKFSIREGGRTVGAGSVSEIIE
EEEEEEEEEECCCCEEEECCCCCEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA