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The map label for this gene is dus1 [H]

Identifier: 138893752

GI number: 138893752

Start: 83502

End: 84503

Strand: Direct

Name: dus1 [H]

Synonym: GTNG_0073

Alternate gene names: 138893752

Gene position: 83502-84503 (Clockwise)

Preceding gene: 138893751

Following gene: 138893753

Centisome position: 2.35

GC content: 49.8

Gene sequence:

>1002_bases
ATGTTTCGCATTGGCGATGTTGAGATTAAAAATCGTGTCGTGCTTGCCCCCATGGCAGGCGTGTGCAACTCGGCGTTCCG
CTTGACTGTCAAAGAATTCGGCGCCGGGCTTGTATGCGCAGAGATGGTGAGCGATAAAGGAATCGTATACAACAATGAAA
AGACGTTAAATATGTTATATATCGATGAGCGGGAAAAGCCGCTCAGCCTGCAAATTTTCGGGGGCGAAAAGGAAACGCTT
GTCAAAGCAGCAAAATTTGTCGATAAAAATACGAATGCGGATATTATCGACATTAACATGGGCTGTCCGGTCCCAAAAGT
TACGAATTGCGACGCGGGAGCGAAATGGCTTCTTGATCCGAATAAAATTTATGACGTGGTTGCCTCCATTGTCGACGCCG
TTGAAAAACCGGTTACGGTCAAAATGCGAATCGGCTGGGACGAACATCATATTTACGCTGTTGAAAACGCGCAAGCAGTT
GAGCGCGCTGGTGGCAAAGCCGTCGCCGTCCATGGGCGGACAAGAGTACAAATGTACGAAGGAAAAGCGGATTGGAACAT
TATTAAACAAGTGAAAGAAGCGGTCAACATCCCAGTCATCGGCAACGGCGATGTCAAAACACCACAAGACGCCAAGCGGA
TGCTTGAAGAAACCGGGGTGGACGGTGTCATGATCGGACGGGCGGCGCTCGGTAACCCGTGGATGATTTATCGCACCGTG
CACTATTTAGAAACAGGGGAGCTTATCCCTGAACCAACGCCGACAGAGAAAATCGATGTGTGCCTGTTGCATTTAGACCG
TTTAATTGCGCTTAAAGGTGAGTATATTGCTGTCAAAGAAATGCGCAAACACGCCGCTTGGTATTTAAAAGGCATTCGCG
GTGCAGCGAAAATCCGCAACGCCATCAACGAGTGTGAGACACGGGACGAACTAGCCGCATTGCTGCTGAAAGTCGCACAG
GATGCAGAGAATCAGGCGGCAGCGAACGCCCGGGCCGTATAG

Upstream 100 bases:

>100_bases
CGATTCGCTCGTCGAGCAAATCGCCGAGCTGTTAAATATATCGGTCGAAGAACTGACGCCGCCGAAAATCGAATCAAACC
AGTAGAAAGGAGGCGTTTTT

Downstream 100 bases:

>100_bases
GAGCGGAATTTGATGAAGCGGATGACTGCCAGCACGCGCTGGCAGTATTTTTCGTAAAAAATCATTAAATGGAGTGAATG
AAGGATGAGCCATGAAGAAT

Product: nitrogen regulation transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MFRIGDVEIKNRVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKGIVYNNEKTLNMLYIDEREKPLSLQIFGGEKETL
VKAAKFVDKNTNADIIDINMGCPVPKVTNCDAGAKWLLDPNKIYDVVASIVDAVEKPVTVKMRIGWDEHHIYAVENAQAV
ERAGGKAVAVHGRTRVQMYEGKADWNIIKQVKEAVNIPVIGNGDVKTPQDAKRMLEETGVDGVMIGRAALGNPWMIYRTV
HYLETGELIPEPTPTEKIDVCLLHLDRLIALKGEYIAVKEMRKHAAWYLKGIRGAAKIRNAINECETRDELAALLLKVAQ
DAENQAAANARAV

Sequences:

>Translated_333_residues
MFRIGDVEIKNRVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKGIVYNNEKTLNMLYIDEREKPLSLQIFGGEKETL
VKAAKFVDKNTNADIIDINMGCPVPKVTNCDAGAKWLLDPNKIYDVVASIVDAVEKPVTVKMRIGWDEHHIYAVENAQAV
ERAGGKAVAVHGRTRVQMYEGKADWNIIKQVKEAVNIPVIGNGDVKTPQDAKRMLEETGVDGVMIGRAALGNPWMIYRTV
HYLETGELIPEPTPTEKIDVCLLHLDRLIALKGEYIAVKEMRKHAAWYLKGIRGAAKIRNAINECETRDELAALLLKVAQ
DAENQAAANARAV
>Mature_333_residues
MFRIGDVEIKNRVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKGIVYNNEKTLNMLYIDEREKPLSLQIFGGEKETL
VKAAKFVDKNTNADIIDINMGCPVPKVTNCDAGAKWLLDPNKIYDVVASIVDAVEKPVTVKMRIGWDEHHIYAVENAQAV
ERAGGKAVAVHGRTRVQMYEGKADWNIIKQVKEAVNIPVIGNGDVKTPQDAKRMLEETGVDGVMIGRAALGNPWMIYRTV
HYLETGELIPEPTPTEKIDVCLLHLDRLIALKGEYIAVKEMRKHAAWYLKGIRGAAKIRNAINECETRDELAALLLKVAQ
DAENQAAANARAV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=235, Percent_Identity=31.4893617021277, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI239788462, Length=231, Percent_Identity=31.6017316017316, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI8923374, Length=247, Percent_Identity=29.1497975708502, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI40807366, Length=233, Percent_Identity=33.0472103004292, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=31.4159292035398, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1789660, Length=311, Percent_Identity=39.2282958199357, Blast_Score=224, Evalue=6e-60,
Organism=Escherichia coli, GI1788462, Length=296, Percent_Identity=33.7837837837838, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI145693211, Length=313, Percent_Identity=26.517571884984, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17543114, Length=228, Percent_Identity=32.0175438596491, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17507177, Length=228, Percent_Identity=31.140350877193, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17510279, Length=247, Percent_Identity=29.9595141700405, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=215, Percent_Identity=29.7674418604651, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323560, Length=232, Percent_Identity=28.8793103448276, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=227, Percent_Identity=25.9911894273128, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323433, Length=284, Percent_Identity=24.6478873239437, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585320, Length=233, Percent_Identity=32.1888412017167, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24580595, Length=256, Percent_Identity=30.859375, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI19920448, Length=256, Percent_Identity=30.859375, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI45549423, Length=260, Percent_Identity=30.3846153846154, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921524, Length=227, Percent_Identity=29.5154185022026, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36749; Mature: 36749

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRIGDVEIKNRVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKGIVYNNEKTLNMLY
CCEECCEEECCEEEEECHHHHCCHHHEEEHHHHCCCCEEHHHHCCCCEEECCCCEEEEEE
IDEREKPLSLQIFGGEKETLVKAAKFVDKNTNADIIDINMGCPVPKVTNCDAGAKWLLDP
EECCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECH
NKIYDVVASIVDAVEKPVTVKMRIGWDEHHIYAVENAQAVERAGGKAVAVHGRTRVQMYE
HHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCHHHHHHCCCCEEEEECCEEEEEEC
GKADWNIIKQVKEAVNIPVIGNGDVKTPQDAKRMLEETGVDGVMIGRAALGNPWMIYRTV
CCCCHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHCCCCEEEECCHHCCCCEEEEEEE
HYLETGELIPEPTPTEKIDVCLLHLDRLIALKGEYIAVKEMRKHAAWYLKGIRGAAKIRN
EHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
AINECETRDELAALLLKVAQDAENQAAANARAV
HHHHHCCHHHHHHHHHHHHHCCCHHHCCCCCCC
>Mature Secondary Structure
MFRIGDVEIKNRVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKGIVYNNEKTLNMLY
CCEECCEEECCEEEEECHHHHCCHHHEEEHHHHCCCCEEHHHHCCCCEEECCCCEEEEEE
IDEREKPLSLQIFGGEKETLVKAAKFVDKNTNADIIDINMGCPVPKVTNCDAGAKWLLDP
EECCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECH
NKIYDVVASIVDAVEKPVTVKMRIGWDEHHIYAVENAQAVERAGGKAVAVHGRTRVQMYE
HHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCHHHHHHCCCCEEEEECCEEEEEEC
GKADWNIIKQVKEAVNIPVIGNGDVKTPQDAKRMLEETGVDGVMIGRAALGNPWMIYRTV
CCCCHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHCCCCEEEECCHHCCCCEEEEEEE
HYLETGELIPEPTPTEKIDVCLLHLDRLIALKGEYIAVKEMRKHAAWYLKGIRGAAKIRN
EHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
AINECETRDELAALLLKVAQDAENQAAANARAV
HHHHHCCHHHHHHHHHHHHHCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]