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The map label for this gene is dacA [H]

Identifier: 138893689

GI number: 138893689

Start: 18607

End: 19965

Strand: Direct

Name: dacA [H]

Synonym: GTNG_0010

Alternate gene names: 138893689

Gene position: 18607-19965 (Clockwise)

Preceding gene: 138893688

Following gene: 138893690

Centisome position: 0.52

GC content: 47.61

Gene sequence:

>1359_bases
GTGGAGACAAAGAAACGAAACTGGTTTGTTTGGCTACTATCAATTTGTCTTTGTTTGACGCTATGGCCGTTCCAAGCAAC
GGTGAAAGCGGAGAGCGCGCCGCTTGATATTCGGGCCGATGCCGCTATTTTAGTGGACGCGCAAACAGGAAGAATTTTGT
ACGAAAAAAATATTGATGCGGTGCTTGGCATTGCCAGTATGACGAAAATGATGACTGAATACTTGCTCCTTGACGCTATT
AAAGCGAAGCGCGTCAAATGGGATCAAACGTATACGCCAAGCGATTACGTGTACCGGCTGTCGCAAGACCGATCGCTGTC
GAACGTCCCTCTGCGCAAGGACGGGCAATATACGGTTCGTGAGCTGTACGAGGCGATGGCCATTTATTCAGCGAATGCAG
CAACGGTCGCGATTGCCGAAATCATTGCCGGATCAGAGAAAAATTTTGTGCGAATGATGAATGAAAAGGGGAAAGAGCTT
GGGCTCAAAGACTATAAATTTGTCAATTCAACCGGGTTAAGCAACAGTGACTTAAAGGGATTCCATCCACAAGGAACAAG
CCAAGACGAGGAAAACGTCATGTCCGCACGGGCGATGGCGACCCTTGCCTACCGGCTGCTGAAAGATCACCCAGAAGTGC
TGGAAACAGCGAGCATTCCAAAGAAACTGTTCCGCGAAGGAACGGATGATGAAATTCGAATGGACAACTGGAACTGGATG
TTGCCAGGGCTTGTGTACGGATATGAAGGGGTAGACGGATTAAAAACTGGCTATACTGAATTTGCCGGCAATTGCTTCAC
TGGAACGGCGAAACGAAACGGTGTCCGCCTTATTTCTGTCGTTATGAACGCGAAAAATGCGAGCGGAAAAACGACGAAAG
AGGCGCGCTTCGAGGAGACGAAAAAGCTATTTAACTATGGATTCAATCAATACTCGCTCCAAACGCTTTATCCGAAGGGA
TATCAGCTGAAAGGAAAAGAGAAGCTTCCAGTCGCGAAAGGGAAAGAAAAAGAGGTTCGTGTCAAGACAGGTAAAAAACT
GTCGCTGTTAGTCAGAAACGGCGAGGAAAAACAATACAAGCCGGTATATGTATTGGACAAGAAAAAAATGACAAATGAAG
GAAAGCTGACTGCGCCGTTGAAAAAGGGAGAGACAGTCGGGTATATGACGCTCGAATATAAAGGAGACGACTCTCTCGCC
TTTTTAAGCCCGGACATGCAAAAAAACATCCGGGTGCCGCTTGTTACGACAGTCGAGGTAGAAAAAGCAAACTGGTTTGT
GCTCTCAATGCGTGCGATCGGTGGGCTGTTTGCCGACTTATGGACGAGCGCGGTCAAAGCGGTGAAAGGTTGGCTGTAA

Upstream 100 bases:

>100_bases
AGGTTGCGCCAATCGACCTAACTTTGACGGAGACATGTTCTCCGTCTATTTTTTTGGCTGTCCATATGTTACAATAACGG
TTGTGTAAGGAGGGTGCGAC

Downstream 100 bases:

>100_bases
AATGACTCCCCTGCCAGGGGAGTTTTTTTCCATTGTGATTTTTGTAAAAATTGAGTTAAAATAAAACAGTATAAATCATC
CTTTTGCGCATATGGCAAAG

Product: D-alanine carboxypeptidase

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
METKKRNWFVWLLSICLCLTLWPFQATVKAESAPLDIRADAAILVDAQTGRILYEKNIDAVLGIASMTKMMTEYLLLDAI
KAKRVKWDQTYTPSDYVYRLSQDRSLSNVPLRKDGQYTVRELYEAMAIYSANAATVAIAEIIAGSEKNFVRMMNEKGKEL
GLKDYKFVNSTGLSNSDLKGFHPQGTSQDEENVMSARAMATLAYRLLKDHPEVLETASIPKKLFREGTDDEIRMDNWNWM
LPGLVYGYEGVDGLKTGYTEFAGNCFTGTAKRNGVRLISVVMNAKNASGKTTKEARFEETKKLFNYGFNQYSLQTLYPKG
YQLKGKEKLPVAKGKEKEVRVKTGKKLSLLVRNGEEKQYKPVYVLDKKKMTNEGKLTAPLKKGETVGYMTLEYKGDDSLA
FLSPDMQKNIRVPLVTTVEVEKANWFVLSMRAIGGLFADLWTSAVKAVKGWL

Sequences:

>Translated_452_residues
METKKRNWFVWLLSICLCLTLWPFQATVKAESAPLDIRADAAILVDAQTGRILYEKNIDAVLGIASMTKMMTEYLLLDAI
KAKRVKWDQTYTPSDYVYRLSQDRSLSNVPLRKDGQYTVRELYEAMAIYSANAATVAIAEIIAGSEKNFVRMMNEKGKEL
GLKDYKFVNSTGLSNSDLKGFHPQGTSQDEENVMSARAMATLAYRLLKDHPEVLETASIPKKLFREGTDDEIRMDNWNWM
LPGLVYGYEGVDGLKTGYTEFAGNCFTGTAKRNGVRLISVVMNAKNASGKTTKEARFEETKKLFNYGFNQYSLQTLYPKG
YQLKGKEKLPVAKGKEKEVRVKTGKKLSLLVRNGEEKQYKPVYVLDKKKMTNEGKLTAPLKKGETVGYMTLEYKGDDSLA
FLSPDMQKNIRVPLVTTVEVEKANWFVLSMRAIGGLFADLWTSAVKAVKGWL
>Mature_452_residues
METKKRNWFVWLLSICLCLTLWPFQATVKAESAPLDIRADAAILVDAQTGRILYEKNIDAVLGIASMTKMMTEYLLLDAI
KAKRVKWDQTYTPSDYVYRLSQDRSLSNVPLRKDGQYTVRELYEAMAIYSANAATVAIAEIIAGSEKNFVRMMNEKGKEL
GLKDYKFVNSTGLSNSDLKGFHPQGTSQDEENVMSARAMATLAYRLLKDHPEVLETASIPKKLFREGTDDEIRMDNWNWM
LPGLVYGYEGVDGLKTGYTEFAGNCFTGTAKRNGVRLISVVMNAKNASGKTTKEARFEETKKLFNYGFNQYSLQTLYPKG
YQLKGKEKLPVAKGKEKEVRVKTGKKLSLLVRNGEEKQYKPVYVLDKKKMTNEGKLTAPLKKGETVGYMTLEYKGDDSLA
FLSPDMQKNIRVPLVTTVEVEKANWFVLSMRAIGGLFADLWTSAVKAVKGWL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=433, Percent_Identity=30.9468822170901, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1786851, Length=397, Percent_Identity=32.2418136020151, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87082022, Length=429, Percent_Identity=29.6037296037296, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI87082054, Length=284, Percent_Identity=26.7605633802817, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 50976; Mature: 50976

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKKRNWFVWLLSICLCLTLWPFQATVKAESAPLDIRADAAILVDAQTGRILYEKNIDA
CCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEECCEEEEEECCCCCEEEECCCHH
VLGIASMTKMMTEYLLLDAIKAKRVKWDQTYTPSDYVYRLSQDRSLSNVPLRKDGQYTVR
HHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCHHHEEEECCCCCCCCCCCCCCCCHHHH
ELYEAMAIYSANAATVAIAEIIAGSEKNFVRMMNEKGKELGLKDYKFVNSTGLSNSDLKG
HHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCC
FHPQGTSQDEENVMSARAMATLAYRLLKDHPEVLETASIPKKLFREGTDDEIRMDNWNWM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEECCCCEE
LPGLVYGYEGVDGLKTGYTEFAGNCFTGTAKRNGVRLISVVMNAKNASGKTTKEARFEET
CCHHEECCCCCCCHHCCHHHHCCCCEECCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHH
KKLFNYGFNQYSLQTLYPKGYQLKGKEKLPVAKGKEKEVRVKTGKKLSLLVRNGEEKQYK
HHHHHCCCCCEEEEEECCCCCEECCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCC
PVYVLDKKKMTNEGKLTAPLKKGETVGYMTLEYKGDDSLAFLSPDMQKNIRVPLVTTVEV
CEEEEECCCCCCCCCEEECCCCCCEEEEEEEEEECCCCEEEECCCHHCCCCCEEEEEEEE
EKANWFVLSMRAIGGLFADLWTSAVKAVKGWL
ECCCEEEEEHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
METKKRNWFVWLLSICLCLTLWPFQATVKAESAPLDIRADAAILVDAQTGRILYEKNIDA
CCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEECCEEEEEECCCCCEEEECCCHH
VLGIASMTKMMTEYLLLDAIKAKRVKWDQTYTPSDYVYRLSQDRSLSNVPLRKDGQYTVR
HHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCHHHEEEECCCCCCCCCCCCCCCCHHHH
ELYEAMAIYSANAATVAIAEIIAGSEKNFVRMMNEKGKELGLKDYKFVNSTGLSNSDLKG
HHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCC
FHPQGTSQDEENVMSARAMATLAYRLLKDHPEVLETASIPKKLFREGTDDEIRMDNWNWM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEECCCCEE
LPGLVYGYEGVDGLKTGYTEFAGNCFTGTAKRNGVRLISVVMNAKNASGKTTKEARFEET
CCHHEECCCCCCCHHCCHHHHCCCCEECCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHH
KKLFNYGFNQYSLQTLYPKGYQLKGKEKLPVAKGKEKEVRVKTGKKLSLLVRNGEEKQYK
HHHHHCCCCCEEEEEECCCCCEECCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCC
PVYVLDKKKMTNEGKLTAPLKKGETVGYMTLEYKGDDSLAFLSPDMQKNIRVPLVTTVEV
CEEEEECCCCCCCCCEEECCCCCCEEEEEEEEEECCCCEEEECCCHHCCCCCEEEEEEEE
EKANWFVLSMRAIGGLFADLWTSAVKAVKGWL
ECCCEEEEEHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]