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The map label for this gene is tyrS

Identifier: 13508408

GI number: 13508408

Start: 791473

End: 792672

Strand: Direct

Name: tyrS

Synonym: MPN669

Alternate gene names: 13508408

Gene position: 791473-792672 (Clockwise)

Preceding gene: 13508406

Following gene: 13508413

Centisome position: 96.95

GC content: 43.42

Gene sequence:

>1200_bases
ATGACAAAAGATCTCCTAGCACTTTTAAAAGAACGGGGCTTATTTGTACAAGCTAACTTTGAAAAGGAATTAAAGCAATT
ATTAAACCAAGGATCATTTGCTTTCTATGTTGGCTTTGACCCCACAGCACCCTCACTCCATATTGGCAACTATGTGCTGC
TCCATGTAGCACAAATCTTCCAAGCAATGGGTCATATTCCCCACGTCTTGTTGGGTAGTGGGACGGCTTTAATAGGTGAT
CCTACTGGCAGAATGGAACTGCGCCAGATGATGAGTCGTGAAACAATTGCCGAAAATACGCGCAACATTAAAAAACAAAT
TAGGCGCTTTTTGGGTAGTAATGTAGTGTTTTGCCAAAACGAGACCTGGTTAAAGAAACTCAATTACATTGAGGTCATCA
GGGAATTGGGACCCTGCTTTTCGGTCAACAAGATGTTAGCGACCGATGCCTTTAGTGCTCGTTGGGAACGCGGTTTAACG
TTGATGGAATTGAACTACATGGTATTACAGGCCTATGACTTCTATTATTTAAACCAAAAGTATGGGGTGCAACTGCAAAT
AGGGGGCAGTGACCAGTGGGCTAATATCCTAGCGGGGGCTGATTTAATTAGACGCAAAACCCAAAAACAGGTGTACGGCA
TGACCACTAACTTATTAGTGAAAGCTAATGGGGAAAAGATGGGTAAGAGTGCTAGTGGAGCTTTATGGTTAGATCCACAA
AAGACCAGTCCGTATGACTTCTACCAGTATTGGATTAACCTTGATGATGCTAGTTTGCAAAAGGTTTTCCTTATGCTCAC
TAAATTAGATACCAAGGCAATTGAAACCCTCTGCAACTTGAAGGGCGCTGCCATTAAGGAGGCCAAGGCAAAACTAGCCT
TCGAGTTAACTGACGCTATTCATGGTACCAAGGCAGCTTTAGTCGCCCAAGCGAAAAGTGCCCGAATCTTTGCCTTTCAA
CCTGATACTGAAACCAAAACCGTGCGAGCTGGCACCAGGTTAGTGGATGTGATTGTTGATTTAGGTTTAGTTGTATCCAG
ATCAGAAGCGCGCCGGGTGATTCAGCAAGGTGGTTTAACGATTAACCAAGAAAAAGTGACTGATGTGGAAATGGTCCTAC
AAGCGAGTTCCCAACCACTAGTGATTGGTAAGGGCAAAAAGCGTTTTGTAACGGTGCAAGTGATAGCTAACACCAAATAA

Upstream 100 bases:

>100_bases
AAAACCATCAACGGTTTGGACAACGCCTTCCCTGCCGGCGCTAGCGTGTGCTGTTGTTTTGTAAATTACTGCCATTGTTA
CTGATATTATATTTAGAAAG

Downstream 100 bases:

>100_bases
AAAACCACCTTTGAGAGTGGTCTTTTAATGAACTTAGTGTTATTTCTTAGCCTTCTTCGCTTTAACTGGTTTGCGCTTGG
AATTGGTTGTGGGTTCAGTA

Product: tyrosyl tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTKDLLALLKERGLFVQANFEKELKQLLNQGSFAFYVGFDPTAPSLHIGNYVLLHVAQIFQAMGHIPHVLLGSGTALIGD
PTGRMELRQMMSRETIAENTRNIKKQIRRFLGSNVVFCQNETWLKKLNYIEVIRELGPCFSVNKMLATDAFSARWERGLT
LMELNYMVLQAYDFYYLNQKYGVQLQIGGSDQWANILAGADLIRRKTQKQVYGMTTNLLVKANGEKMGKSASGALWLDPQ
KTSPYDFYQYWINLDDASLQKVFLMLTKLDTKAIETLCNLKGAAIKEAKAKLAFELTDAIHGTKAALVAQAKSARIFAFQ
PDTETKTVRAGTRLVDVIVDLGLVVSRSEARRVIQQGGLTINQEKVTDVEMVLQASSQPLVIGKGKKRFVTVQVIANTK

Sequences:

>Translated_399_residues
MTKDLLALLKERGLFVQANFEKELKQLLNQGSFAFYVGFDPTAPSLHIGNYVLLHVAQIFQAMGHIPHVLLGSGTALIGD
PTGRMELRQMMSRETIAENTRNIKKQIRRFLGSNVVFCQNETWLKKLNYIEVIRELGPCFSVNKMLATDAFSARWERGLT
LMELNYMVLQAYDFYYLNQKYGVQLQIGGSDQWANILAGADLIRRKTQKQVYGMTTNLLVKANGEKMGKSASGALWLDPQ
KTSPYDFYQYWINLDDASLQKVFLMLTKLDTKAIETLCNLKGAAIKEAKAKLAFELTDAIHGTKAALVAQAKSARIFAFQ
PDTETKTVRAGTRLVDVIVDLGLVVSRSEARRVIQQGGLTINQEKVTDVEMVLQASSQPLVIGKGKKRFVTVQVIANTK
>Mature_398_residues
TKDLLALLKERGLFVQANFEKELKQLLNQGSFAFYVGFDPTAPSLHIGNYVLLHVAQIFQAMGHIPHVLLGSGTALIGDP
TGRMELRQMMSRETIAENTRNIKKQIRRFLGSNVVFCQNETWLKKLNYIEVIRELGPCFSVNKMLATDAFSARWERGLTL
MELNYMVLQAYDFYYLNQKYGVQLQIGGSDQWANILAGADLIRRKTQKQVYGMTTNLLVKANGEKMGKSASGALWLDPQK
TSPYDFYQYWINLDDASLQKVFLMLTKLDTKAIETLCNLKGAAIKEAKAKLAFELTDAIHGTKAALVAQAKSARIFAFQP
DTETKTVRAGTRLVDVIVDLGLVVSRSEARRVIQQGGLTINQEKVTDVEMVLQASSQPLVIGKGKKRFVTVQVIANTK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=412, Percent_Identity=32.2815533980583, Blast_Score=199, Evalue=4e-51,
Organism=Escherichia coli, GI1787925, Length=419, Percent_Identity=37.2315035799523, Blast_Score=272, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI25145802, Length=389, Percent_Identity=30.8483290488432, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI25145805, Length=329, Percent_Identity=28.5714285714286, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6325160, Length=405, Percent_Identity=32.8395061728395, Blast_Score=203, Evalue=4e-53,
Organism=Drosophila melanogaster, GI20130383, Length=440, Percent_Identity=28.4090909090909, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_MYCPN (P75122)

Other databases:

- EMBL:   U00089
- PIR:   S73499
- RefSeq:   NP_110358.1
- ProteinModelPortal:   P75122
- SMR:   P75122
- GeneID:   877033
- GenomeReviews:   U00089_GR
- KEGG:   mpn:MPN669
- HOGENOM:   HBG288125
- OMA:   HEVIILL
- ProtClustDB:   PRK05912
- BioCyc:   MPNE272634:MPN669-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 44651; Mature: 44519

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 36-36 BINDING 166-166 BINDING 170-170 BINDING 229-229

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKDLLALLKERGLFVQANFEKELKQLLNQGSFAFYVGFDPTAPSLHIGNYVLLHVAQIF
CCHHHHHHHHHCCEEEEECHHHHHHHHHCCCCEEEEEECCCCCCCEECCCHHHHHHHHHH
QAMGHIPHVLLGSGTALIGDPTGRMELRQMMSRETIAENTRNIKKQIRRFLGSNVVFCQN
HHHCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
ETWLKKLNYIEVIRELGPCFSVNKMLATDAFSARWERGLTLMELNYMVLQAYDFYYLNQK
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEHEEEEEEEEECCC
YGVQLQIGGSDQWANILAGADLIRRKTQKQVYGMTTNLLVKANGEKMGKSASGALWLDPQ
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCHHCCCCCCCCEEECCC
KTSPYDFYQYWINLDDASLQKVFLMLTKLDTKAIETLCNLKGAAIKEAKAKLAFELTDAI
CCCCCEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
HGTKAALVAQAKSARIFAFQPDTETKTVRAGTRLVDVIVDLGLVVSRSEARRVIQQGGLT
CCCHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEECHHHHHHHHHCCCE
INQEKVTDVEMVLQASSQPLVIGKGKKRFVTVQVIANTK
ECHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEEECCC
>Mature Secondary Structure 
TKDLLALLKERGLFVQANFEKELKQLLNQGSFAFYVGFDPTAPSLHIGNYVLLHVAQIF
CHHHHHHHHHCCEEEEECHHHHHHHHHCCCCEEEEEECCCCCCCEECCCHHHHHHHHHH
QAMGHIPHVLLGSGTALIGDPTGRMELRQMMSRETIAENTRNIKKQIRRFLGSNVVFCQN
HHHCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
ETWLKKLNYIEVIRELGPCFSVNKMLATDAFSARWERGLTLMELNYMVLQAYDFYYLNQK
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEHEEEEEEEEECCC
YGVQLQIGGSDQWANILAGADLIRRKTQKQVYGMTTNLLVKANGEKMGKSASGALWLDPQ
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCHHCCCCCCCCEEECCC
KTSPYDFYQYWINLDDASLQKVFLMLTKLDTKAIETLCNLKGAAIKEAKAKLAFELTDAI
CCCCCEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
HGTKAALVAQAKSARIFAFQPDTETKTVRAGTRLVDVIVDLGLVVSRSEARRVIQQGGLT
CCCHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEECHHHHHHHHHCCCE
INQEKVTDVEMVLQASSQPLVIGKGKKRFVTVQVIANTK
ECHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633