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The map label for this gene is fmt [H]

Identifier: 13508282

GI number: 13508282

Start: 661848

End: 662783

Strand: Direct

Name: fmt [H]

Synonym: MPN543

Alternate gene names: 13508282

Gene position: 661848-662783 (Clockwise)

Preceding gene: 13508281

Following gene: 13508283

Centisome position: 81.07

GC content: 37.93

Gene sequence:

>936_bases
ATGATTAAAGTGGTTTTCTTTGGGACATCCACCCTTTCAAAGTGTTGTTTAGAAGCAATTTTTCAAGATCCTGAGTTTGT
GGTTTGCGGCGTAGTGACCCAGCCCGATAAGGTAAACGAGCGCAACAATAAAATTAACTTTTCTGCAGTTAAACAATTTT
GTATAGAAAACAACATTCCCTGTTTTCAACCAGAAAAAAACATTCAGATTAAAACAGAGTTAGCCCAACTCCAAGCTGAT
ATTGGCGTTTGTGTAGCCTTTGGCCAATACATCCACAACGATATTATTAACCTCTTTCCTTACAAGATTGCCAACCTACA
CCCCTCAAAATTACCACTACTGCGTGGTGGAGCACCATTGCACTGAACCATTATTAACGGTTTTACGACCTCTAGTTTAA
GTGTGATTGAACTCGTACAAAAAATGGATGCTGGTCCGATTTGAAAGCAAAAGGACTTTAAGGTGAATCCCAATTGGAAT
ACCGGTGATCTATTTGAATACGTGCAAACTCACGCACCGCAATTTCTGATCCAGTGCCTTAAGGAAATTGTAAGTGGTAA
GAGTCAATGAAAAGAGCAAATTAATCCACCTACTTTTGGTTTGAATATTAAAAAGGAACAAGAAAGACTTGACCTCAATT
TGGAACCAAAAGCATTCATCAATTGGGTGAAAGGGTTGGCACCTAAACCTGGTGGTTGACTGGAATTTGAAGGACAAAAC
ATTAAAATTCTCCAAGCAACTTACTTGGGCAAAATGACAAGTACAACCGCAGTTGGTCAAATCACTAAAATTTCTAAACA
AGGTATAGAAATCGCCTTAGCTAATGATGAAATTGTTCTACTCCAAATAATTCAAATTCCGGGAAAGCGGGCAATGGGAG
TGTCAGAAATAATTAACGGTAAACATCCTTTTGCTGAAGGTAAATTTTTTCATTAA

Upstream 100 bases:

>100_bases
GAAAAACGAAAGGAAAAAGAAGAGAAACGCAAGCGGGAATTGGCAGCACGTCAGTTAAAAAGACAAGAAAAGAAAAAACA
AAAAACTTCCAAATAACAAG

Downstream 100 bases:

>100_bases
TGCTCACCAAGGCCTTTTTTTTAAAAAACTTTGATCGCTCAAAGGAGTTGATTCCTCTGTCTTACGCTGATGTTTTTGGC
GCTGCTAGTCAATTGTTAAA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MIKVVFFGTSTLSKCCLEAIFQDPEFVVCGVVTQPDKVNERNNKINFSAVKQFCIENNIPCFQPEKNIQIKTELAQLQAD
IGVCVAFGQYIHNDIINLFPYKIANLHPSKLPLLRGGAPLHWTIINGFTTSSLSVIELVQKMDAGPIWKQKDFKVNPNWN
TGDLFEYVQTHAPQFLIQCLKEIVSGKSQWKEQINPPTFGLNIKKEQERLDLNLEPKAFINWVKGLAPKPGGWLEFEGQN
IKILQATYLGKMTSTTAVGQITKISKQGIEIALANDEIVLLQIIQIPGKRAMGVSEIINGKHPFAEGKFFH

Sequences:

>Translated_311_residues
MIKVVFFGTSTLSKCCLEAIFQDPEFVVCGVVTQPDKVNERNNKINFSAVKQFCIENNIPCFQPEKNIQIKTELAQLQAD
IGVCVAFGQYIHNDIINLFPYKIANLHPSKLPLLRGGAPLH*TIINGFTTSSLSVIELVQKMDAGPI*KQKDFKVNPNWN
TGDLFEYVQTHAPQFLIQCLKEIVSGKSQ*KEQINPPTFGLNIKKEQERLDLNLEPKAFINWVKGLAPKPGG*LEFEGQN
IKILQATYLGKMTSTTAVGQITKISKQGIEIALANDEIVLLQIIQIPGKRAMGVSEIINGKHPFAEGKFFH
>Mature_311_residues
MIKVVFFGTSTLSKCCLEAIFQDPEFVVCGVVTQPDKVNERNNKINFSAVKQFCIENNIPCFQPEKNIQIKTELAQLQAD
IGVCVAFGQYIHNDIINLFPYKIANLHPSKLPLLRGGAPLH*TIINGFTTSSLSVIELVQKMDAGPI*KQKDFKVNPNWN
TGDLFEYVQTHAPQFLIQCLKEIVSGKSQ*KEQINPPTFGLNIKKEQERLDLNLEPKAFINWVKGLAPKPGG*LEFEGQN
IKILQATYLGKMTSTTAVGQITKISKQGIEIALANDEIVLLQIIQIPGKRAMGVSEIINGKHPFAEGKFFH

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI164663775, Length=191, Percent_Identity=27.2251308900524, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1789683, Length=309, Percent_Identity=29.126213592233, Blast_Score=128, Evalue=6e-31,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 34088; Mature: 34088

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVVFFGTSTLSKCCLEAIFQDPEFVVCGVVTQPDKVNERNNKINFSAVKQFCIENNIP
CEEEEEECCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEECHHHHHHHHHHCCCC
CFQPEKNIQIKTELAQLQADIGVCVAFGQYIHNDIINLFPYKIANLHPSKLPLLRGGAPL
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEEECCCHH
HTIINGFTTSSLSVIELVQKMDAGPIKQKDFKVNPNWNTGDLFEYVQTHAPQFLIQCLKE
HHHHHCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHCCHHHHHHHHHH
IVSGKSQKEQINPPTFGLNIKKEQERLDLNLEPKAFINWVKGLAPKPGGLEFEGQNIKIL
HHCCCCCHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEEE
QATYLGKMTSTTAVGQITKISKQGIEIALANDEIVLLQIIQIPGKRAMGVSEIINGKHPF
EEEHHCCHHCHHHHHHHHHHHHCCEEEEEECCCEEEEEEECCCCCCCCCHHHHHCCCCCC
AEGKFFH
CCCCCCC
>Mature Secondary Structure
MIKVVFFGTSTLSKCCLEAIFQDPEFVVCGVVTQPDKVNERNNKINFSAVKQFCIENNIP
CEEEEEECCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEECHHHHHHHHHHCCCC
CFQPEKNIQIKTELAQLQADIGVCVAFGQYIHNDIINLFPYKIANLHPSKLPLLRGGAPL
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEEECCCHH
HTIINGFTTSSLSVIELVQKMDAGPIKQKDFKVNPNWNTGDLFEYVQTHAPQFLIQCLKE
HHHHHCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHCCHHHHHHHHHH
IVSGKSQKEQINPPTFGLNIKKEQERLDLNLEPKAFINWVKGLAPKPGGLEFEGQNIKIL
HHCCCCCHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEEE
QATYLGKMTSTTAVGQITKISKQGIEIALANDEIVLLQIIQIPGKRAMGVSEIINGKHPF
EEEHHCCHHCHHHHHHHHHHHHCCEEEEEECCCEEEEEEECCCCCCCCCHHHHHCCCCCC
AEGKFFH
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]