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The map label for this gene is araD

Identifier: 13508237

GI number: 13508237

Start: 604897

End: 605625

Strand: Direct

Name: araD

Synonym: MPN498

Alternate gene names: 13508237

Gene position: 604897-605625 (Clockwise)

Preceding gene: 13508230

Following gene: 13508238

Centisome position: 74.09

GC content: 41.7

Gene sequence:

>729_bases
ATGGATCAAAAAATGATAAATGACCTCAAAGAACAGGTTTTTCAAACGAATTTATTACTACCCAAATATGGCTTAGTCAT
CCACACTTGGGGTAATGTTTCGATGATTGCCCCGAACCGTCAGTTCTTTGTCATTAAACCAAGTGGTGTGAGCTATGACA
AGATGCGTGCCCAAGATATGGTAGTGGTCGATTTGGACAACAATGTTCTAGATACCAACGGCCTCAAACCATCAAGTGAT
ACCCCAACCCATGCGTTAATGTACAAACATTGTCCAGATATTAAAGCTATTGTGCACACCCACTCTACTTTCGCGACATC
GTTTGCTCAAGCCGATAAGCCGATTCCTTGTCTTGGTACTACCCATGCTGATAACTTCTTTGGACCAATCCCGTGTACTA
GGGCTTTAAGTGACAGTGAAATTAATGGAGCTTATGAACATAATACTGGTTTAGTCATTCTGGAACACCTCAAAAACAAC
CAGGTTGATGTCAATGCTTGTGCGGCCATCTTAGTCAAAGAACACGGTTCGTTTGTTTGGTCCAATAAGAATGGCAAGGA
TGCAGTGGACCGGGCGTTAACTTTAGAACAAGTGGCCCAAATGGCCTTATATACCCAAATGATCAATCCCCACATGAAAG
AAGCAAACCCAGCGTTACAACAAAAGCACTATAACCGCAAACATGGTAAAGATGCTTACTATGGACAAGATACTAAGCAA
GAAGATTAA

Upstream 100 bases:

>100_bases
AAATGGTTTAAGTGTATTGAAATAACCCTAATAATGTGTAAGCTACTGGATAATTTGCCATTAACAGTTGTAAAGCTGGC
AACTATTTTTAGAATCAGCC

Downstream 100 bases:

>100_bases
AGTGAACTGGTTGGGCGGGGTGTTTTGGTTATTACCTAACTTACTCGATCTGTTTTCCGCTAGCAAACGTAAAGCGAGTG
TGCGACCCTACCAAAGCTTA

Product: L-ribulose-5-phosphate 4-epimerase

Products: NA

Alternate protein names: L-ascorbate utilization protein F; Phosphoribulose isomerase

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MDQKMINDLKEQVFQTNLLLPKYGLVIHTWGNVSMIAPNRQFFVIKPSGVSYDKMRAQDMVVVDLDNNVLDTNGLKPSSD
TPTHALMYKHCPDIKAIVHTHSTFATSFAQADKPIPCLGTTHADNFFGPIPCTRALSDSEINGAYEHNTGLVILEHLKNN
QVDVNACAAILVKEHGSFVWSNKNGKDAVDRALTLEQVAQMALYTQMINPHMKEANPALQQKHYNRKHGKDAYYGQDTKQ
ED

Sequences:

>Translated_242_residues
MDQKMINDLKEQVFQTNLLLPKYGLVIHTWGNVSMIAPNRQFFVIKPSGVSYDKMRAQDMVVVDLDNNVLDTNGLKPSSD
TPTHALMYKHCPDIKAIVHTHSTFATSFAQADKPIPCLGTTHADNFFGPIPCTRALSDSEINGAYEHNTGLVILEHLKNN
QVDVNACAAILVKEHGSFVWSNKNGKDAVDRALTLEQVAQMALYTQMINPHMKEANPALQQKHYNRKHGKDAYYGQDTKQ
ED
>Mature_242_residues
MDQKMINDLKEQVFQTNLLLPKYGLVIHTWGNVSMIAPNRQFFVIKPSGVSYDKMRAQDMVVVDLDNNVLDTNGLKPSSD
TPTHALMYKHCPDIKAIVHTHSTFATSFAQADKPIPCLGTTHADNFFGPIPCTRALSDSEINGAYEHNTGLVILEHLKNN
QVDVNACAAILVKEHGSFVWSNKNGKDAVDRALTLEQVAQMALYTQMINPHMKEANPALQQKHYNRKHGKDAYYGQDTKQ
ED

Specific function: Catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate. Is involved in the anaerobic L-ascorbate utilization

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily

Homologues:

Organism=Escherichia coli, GI1786247, Length=232, Percent_Identity=51.2931034482759, Blast_Score=242, Evalue=2e-65,
Organism=Escherichia coli, GI1790642, Length=231, Percent_Identity=51.9480519480519, Blast_Score=239, Evalue=1e-64,
Organism=Escherichia coli, GI1790008, Length=232, Percent_Identity=48.2758620689655, Blast_Score=224, Evalue=4e-60,
Organism=Escherichia coli, GI1789164, Length=210, Percent_Identity=29.0476190476191, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 136 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): ULAF_MYCPN (P75289)

Other databases:

- EMBL:   U00089
- PIR:   S73671
- RefSeq:   NP_110186.1
- ProteinModelPortal:   P75289
- SMR:   P75289
- GeneID:   877253
- GenomeReviews:   U00089_GR
- KEGG:   mpn:MPN498
- HOGENOM:   HBG541069
- OMA:   DSEINGA
- ProtClustDB:   PRK08193
- BioCyc:   MPNE272634:MPN498-MONOMER
- BRENDA:   5.1.3.4
- InterPro:   IPR001303
- Gene3D:   G3DSA:3.40.225.10

Pfam domain/function: PF00596 Aldolase_II; SSF53639 Aldolase_II_N

EC number: =5.1.3.4

Molecular weight: Translated: 27092; Mature: 27092

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQKMINDLKEQVFQTNLLLPKYGLVIHTWGNVSMIAPNRQFFVIKPSGVSYDKMRAQDM
CCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCEEEEECCCCCCHHHHCCCCE
VVVDLDNNVLDTNGLKPSSDTPTHALMYKHCPDIKAIVHTHSTFATSFAQADKPIPCLGT
EEEECCCCEECCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
THADNFFGPIPCTRALSDSEINGAYEHNTGLVILEHLKNNQVDVNACAAILVKEHGSFVW
CCCCCCCCCCCCHHCCCCCCCCCEEECCCCEEEEEECCCCCEEHHHHHEEEEECCCCEEE
SNKNGKDAVDRALTLEQVAQMALYTQMINPHMKEANPALQQKHYNRKHGKDAYYGQDTKQ
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHCCHHCCCCCCCCCCCCC
ED
CC
>Mature Secondary Structure
MDQKMINDLKEQVFQTNLLLPKYGLVIHTWGNVSMIAPNRQFFVIKPSGVSYDKMRAQDM
CCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCEEEEECCCCCCHHHHCCCCE
VVVDLDNNVLDTNGLKPSSDTPTHALMYKHCPDIKAIVHTHSTFATSFAQADKPIPCLGT
EEEECCCCEECCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
THADNFFGPIPCTRALSDSEINGAYEHNTGLVILEHLKNNQVDVNACAAILVKEHGSFVW
CCCCCCCCCCCCHHCCCCCCCCCEEECCCCEEEEEECCCCCEEHHHHHEEEEECCCCEEE
SNKNGKDAVDRALTLEQVAQMALYTQMINPHMKEANPALQQKHYNRKHGKDAYYGQDTKQ
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHCCHHCCCCCCCCCCCCC
ED
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633